FastQCFastQC Report
Mon 15 Aug 2016
SRR2921311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070130
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.126433237083345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10810.10101576443983441No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.068.8687741
GTATCAA19450.051.9064141
GTACATG44200.037.3027421
TCAACGC25450.036.5182954
TACATGG46750.035.682072
ACATGGG48050.034.8406753
CAACGCA26950.034.70685
TATCAAC28200.034.0134282
AACGCAG28100.033.927226
TATACCG1251.2651726E-633.3627365
ATCAACG29250.031.9777163
CATGGGG44150.031.5761244
ATGGGGG25200.028.84255
ATACCGA1254.8351867E-528.6006476
TATACAG4750.026.3390035
ACGCAGA35550.026.3095477
CGCAGAG37650.025.0003138
CCCTATA2052.2377972E-623.2492942
TGGGGGG17100.022.9975976
CTATACT3455.2750693E-1122.4490474
Produced by FastQC (version 0.11.5)