FastQCFastQC Report
Mon 15 Aug 2016
SRR2921310_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921310_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359075
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.059.9977071
GTATCAA20850.051.660891
GTACATG70400.042.0119741
TCAACGC25450.041.1526954
CAACGCA26550.039.446235
TACATGG73650.039.1870352
ACATGGG73050.038.6942833
AACGCAG28700.036.4885146
TATCAAC29550.036.2483632
CATGGGG66600.035.740384
ATCAACG29650.035.5240063
ATGGGGG42550.035.1019745
TGGGGGG30700.030.0413046
GAGTACA47450.028.8456941
ACGCAGA36300.028.8447997
AGTACAT47050.028.457472
CGCAGAG38200.027.253378
GTATAGG4600.027.1675721
GCAGAGT43750.023.2521749
AGAGTAC38950.021.5411595
Produced by FastQC (version 0.11.5)