##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921310_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1359075
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.585104574802717	33.0	31.0	34.0	27.0	34.0
2	30.586520979342566	33.0	31.0	34.0	27.0	34.0
3	30.74476243032945	33.0	31.0	34.0	27.0	34.0
4	34.17750823170171	37.0	35.0	37.0	31.0	37.0
5	34.18867465003771	37.0	35.0	37.0	30.0	37.0
6	34.25156080422346	37.0	35.0	37.0	31.0	37.0
7	34.13986203851884	37.0	35.0	37.0	30.0	37.0
8	34.11907952099774	37.0	35.0	37.0	30.0	37.0
9	35.71909718006732	39.0	37.0	39.0	30.0	39.0
10-11	35.77256148483343	39.0	37.0	39.0	30.0	39.0
12-13	35.724529551349264	39.0	37.0	39.0	30.0	39.0
14-15	37.053497047624305	41.0	38.0	41.0	31.0	41.0
16-17	37.0429545830804	41.0	38.0	41.0	31.0	41.0
18-19	37.02736714309365	41.0	38.0	41.0	30.0	41.0
20-21	37.07008332873462	41.0	38.0	41.0	30.0	41.0
22-23	37.024885675919286	41.0	38.0	41.0	30.0	41.0
24-25	37.016837554954655	41.0	38.0	41.0	30.0	41.0
26-27	36.9954973051524	40.5	38.0	41.0	30.0	41.0
28-29	36.934885859867926	40.0	38.0	41.0	30.0	41.0
30-31	36.859631734819644	40.0	38.0	41.0	30.0	41.0
32-33	36.783934293545244	40.0	38.0	41.0	30.0	41.0
34-35	36.67410554973051	40.0	38.0	41.0	30.0	41.0
36-37	36.56286518404062	40.0	37.0	41.0	30.0	41.0
38-39	36.45739344774938	40.0	37.0	41.0	29.0	41.0
40-41	36.35399002998363	40.0	37.0	41.0	29.0	41.0
42-43	36.14977429501683	40.0	36.5	41.0	28.0	41.0
44-45	35.89324283060169	40.0	36.0	41.0	27.5	41.0
46-47	35.67209830215404	40.0	35.5	41.0	27.0	41.0
48-49	35.459495612824895	40.0	35.0	41.0	26.0	41.0
50-51	35.275412688777294	39.0	35.0	41.0	26.0	41.0
52-53	35.07911447123963	39.0	35.0	41.0	26.0	41.0
54-55	34.87725143939812	39.0	35.0	41.0	25.5	41.0
56-57	34.62899766385225	38.5	34.5	40.0	25.0	41.0
58-59	34.35769622721337	38.0	34.0	40.0	24.0	41.0
60-61	34.06299541967883	37.5	34.0	40.0	23.0	41.0
62-63	33.73380534554752	37.0	34.0	40.0	22.5	41.0
64-65	33.38831558228942	36.5	33.5	39.5	22.0	41.0
66-67	33.048094108125014	36.0	33.0	39.0	21.5	41.0
68-69	32.69489910417012	35.5	33.0	39.0	20.5	40.0
70-71	32.64609936905616	35.0	33.0	38.5	21.0	40.0
72-73	32.35340249802255	35.0	33.0	37.0	20.0	39.5
74-75	32.02121332523959	35.0	33.0	37.0	20.0	39.0
76-77	31.721406103415926	35.0	33.0	36.5	18.5	39.0
78-79	31.43087210050954	35.0	33.0	36.0	18.0	37.5
80-81	31.154404282324375	35.0	33.0	35.5	16.5	37.0
82-83	30.927315637474017	35.0	32.0	35.0	14.0	37.0
84-85	30.70970844140316	35.0	32.0	35.0	11.0	36.0
86-87	30.537199565881206	35.0	32.0	35.0	7.5	36.0
88-89	30.39309162481835	35.0	32.0	35.0	7.0	36.0
90-91	30.26368154811177	35.0	32.0	35.0	6.0	35.0
92-93	30.147954675054727	35.0	32.0	35.0	2.0	35.0
94-95	30.02061328477089	35.0	31.5	35.0	2.0	35.0
96-97	29.918541287272593	35.0	31.0	35.0	2.0	35.0
98-99	29.832704964773836	35.0	31.0	35.0	2.0	35.0
100-101	29.741609182716186	35.0	31.0	35.0	2.0	35.0
102-103	28.888428158858048	33.5	29.5	34.5	2.0	35.0
104-105	29.493178448577158	34.0	31.0	35.0	2.0	35.0
106-107	29.568771774920442	34.0	31.0	35.0	2.0	35.0
108-109	29.50100509537737	34.0	31.0	35.0	2.0	35.0
110-111	29.419004469951986	34.0	31.0	35.0	2.0	35.0
112-113	29.328049224656475	34.0	31.0	35.0	2.0	35.0
114-115	29.19691407758954	34.0	31.0	35.0	2.0	35.0
116-117	29.057680775527473	34.0	30.0	35.0	2.0	35.0
118-119	29.061702628626087	34.0	30.5	35.0	2.0	35.0
120-121	29.239135809282047	35.0	31.0	35.0	2.0	35.0
122-123	29.096211025881573	35.0	31.0	35.0	2.0	35.0
124-125	28.495179809797108	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45070.0
3	6661.0
4	4351.0
5	3639.0
6	4101.0
7	4520.0
8	4613.0
9	4738.0
10	5428.0
11	5836.0
12	5896.0
13	5692.0
14	5778.0
15	5323.0
16	4604.0
17	4278.0
18	3986.0
19	3667.0
20	3795.0
21	3870.0
22	4292.0
23	5590.0
24	7951.0
25	10091.0
26	10852.0
27	11735.0
28	14002.0
29	17106.0
30	20978.0
31	26368.0
32	34292.0
33	47427.0
34	75502.0
35	124731.0
36	263610.0
37	435065.0
38	113566.0
39	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.595357329198535	17.313832860024448	11.698664996655317	25.392144814121703
2	16.724518427324966	19.279979218990192	38.319328473147685	25.676173880537156
3	20.542983628368074	22.902182295560642	28.269616817863387	28.28521725820789
4	13.65566053356038	14.33157039948959	34.47118239705528	37.54158666989474
5	14.354200776320388	38.324546073327326	32.545558233227254	14.77569491712504
6	35.14122006778563	33.470204865698335	16.479121509978327	14.909453556537708
7	30.906790923848813	31.926143312762807	18.73188795071315	18.43517781267523
8	27.995010707110847	35.42508333885247	18.42329514095856	18.156610813078128
9	28.05554226930053	16.1624471564757	17.764990268329207	38.01702030589456
10-11	27.094755114437646	25.615879361708473	25.849107210857625	21.440258312996264
12-13	26.359721028527026	22.8027832393421	26.467815851748945	24.36967988038193
14-15	23.895500988411687	24.241307109610496	23.56555213269183	28.297639769285986
16-17	24.47377367153882	27.40405558139124	24.20298951594389	23.91918123112605
18-19	24.4019041935599	26.808452241187545	25.65249240158484	23.13715116366771
20-21	25.08720518466602	25.66773914051247	25.59003537508071	23.655020299740805
22-23	25.074613247309802	25.05787309566978	25.537745178836328	24.32976847818409
24-25	24.51831398396986	25.286230157781166	25.62736271640413	24.568093141844844
26-27	24.209319951141264	25.659188238583685	25.73541964062339	24.39607216965166
28-29	24.275771028192114	26.228792263706247	25.429195207936438	24.066241500165194
30-31	25.07843045636647	25.623534545272598	25.46065820877236	23.83737678958857
32-33	24.558019881976747	25.575040499437257	25.513339335430263	24.35360028315573
34-35	24.555951115990617	25.862894894081702	25.76583623503648	23.815317754891197
36-37	24.45179553518737	25.93654662092655	25.672783009635154	23.93887483425093
38-39	24.604326552146997	26.06372939126058	25.554634521981733	23.777309534610687
40-41	24.58682186172256	25.803618601625633	25.6076993152207	24.001860221431105
42-43	24.254545574956044	25.683367715573336	25.5264125140316	24.53567419543903
44-45	24.061044177888906	25.85374504316831	25.745171205232147	24.340039573710634
46-47	24.01460383080131	25.729630448611935	25.691145093834727	24.56462062675203
48-49	23.992912268721557	26.062828558141177	25.71627882334242	24.227980349794844
50-51	24.116114790286975	25.960779985283295	25.73440029433407	24.188704930095657
52-53	24.288964565225708	25.91191784571642	25.5565472949307	24.24257029412717
54-55	23.813951092625263	25.571055134391347	26.025029479450843	24.58996429353255
56-57	24.080164564660468	25.85259748215481	25.530149645377286	24.53708830780744
58-59	24.04038322859792	26.0834596535637	25.68639715080428	24.1897599670341
60-61	24.546233185407353	25.86958329316938	25.73735497747262	23.846828543950647
62-63	24.35439709021553	25.882909578432983	25.7404887855123	24.022204545839188
64-65	24.146129063233634	25.534912178268126	25.757129249763526	24.56182950873471
66-67	24.127525482469935	25.881506744737486	25.549981696403112	24.440986076389464
68-69	23.91805838444965	25.864758585775338	25.675500665198914	24.541682364576097
70-71	23.957314672759313	25.68117349698127	25.85931722607918	24.502194604180232
72-73	24.063587183668183	26.08549126721098	25.50963344066589	24.341288108454943
74-75	23.860121984331435	26.098745646701648	25.593825582567526	24.447306786399388
76-77	23.8276636716097	25.640444527183988	25.72075993162705	24.811131869579263
78-79	23.751413684837132	25.6168890177383	25.98365607411719	24.648041223307384
80-81	24.107205598684654	25.90554243144117	25.49440466331404	24.49284730656014
82-83	24.167625702352606	25.963499739515118	25.644552922581536	24.22432163555074
84-85	24.154074270303287	26.01226677851732	25.428877335894125	24.404781615285273
86-87	23.760712942802247	26.278764991865266	25.58802990757086	24.372492157761627
88-89	23.807014994288153	25.839623110077685	25.652318678751378	24.701043216882784
90-91	24.168410890431595	25.578776307303226	25.774433512054184	24.478379290210995
92-93	24.1786984416886	25.74929855015302	25.66519079509423	24.40681221306415
94-95	24.15110139468418	25.666979278231526	25.63059173358872	24.55132759349557
96-97	23.840307176301863	25.646800234591332	25.711003790401328	24.80188879870548
98-99	23.680842100615717	25.6831489735887	25.67751976202784	24.958489163767744
100-101	23.638287554756584	25.901510630485312	25.751212867990365	24.708988946767736
102-103	23.521172620460153	25.921085792388933	25.81895243403734	24.738789153113576
104-105	23.606840248268046	25.907145984407826	25.693388226031356	24.79262554129277
106-107	23.64518172780675	25.756282455336088	25.88991179433444	24.708624022522724
108-109	23.54506786108355	25.81230392140631	25.85097235850608	24.79165585900406
110-111	23.418013296006304	26.261875179132577	25.72474419224131	24.59536733261981
112-113	23.447139384021288	26.34186112223033	25.655036614942667	24.55596287880572
114-115	23.47607199317462	26.43653211022	25.594102625703112	24.49329327090226
116-117	23.35024110778237	26.220650687489215	25.677362578497537	24.75174562623088
118-119	23.421045359331906	26.04201555271841	25.784498497602243	24.75244059034744
120-121	23.25680908798234	26.165699305523617	25.946851860368852	24.63063974612519
122-123	23.358944379488893	26.27826516924702	25.686233535361858	24.67655691590222
124-125	23.26014151750353	26.155168906029893	25.667256169638524	24.917433406828053
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	473.0
1	416.0
2	335.0
3	262.5
4	187.5
5	153.0
6	156.0
7	179.0
8	224.5
9	260.5
10	262.0
11	272.5
12	297.5
13	340.0
14	387.5
15	417.5
16	445.0
17	480.5
18	539.5
19	614.0
20	661.0
21	705.0
22	828.0
23	1052.0
24	1280.0
25	1569.5
26	2151.5
27	2953.5
28	3753.0
29	4796.0
30	6275.5
31	7992.5
32	10050.5
33	12323.5
34	14588.5
35	17012.5
36	20030.0
37	22860.5
38	25325.0
39	27936.5
40	30627.5
41	33130.0
42	35950.5
43	39997.5
44	43816.0
45	47390.0
46	50555.5
47	53693.5
48	57813.5
49	60952.5
50	62445.0
51	63452.5
52	63016.0
53	61414.5
54	60814.0
55	59033.5
56	54742.5
57	49650.5
58	45135.0
59	40131.5
60	34020.5
61	28370.0
62	23168.5
63	17773.0
64	13035.5
65	9690.5
66	7302.0
67	5595.0
68	4194.0
69	3094.5
70	2345.0
71	1771.0
72	1247.5
73	890.0
74	676.0
75	508.0
76	337.5
77	202.0
78	132.5
79	85.0
80	63.0
81	47.5
82	31.5
83	21.5
84	13.5
85	12.0
86	8.5
87	5.5
88	9.0
89	10.5
90	8.5
91	6.5
92	6.0
93	4.0
94	1.5
95	1.5
96	1.5
97	1.5
98	2.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014863050236374003
2	0.01044828283943123
3	0.010227544469584092
4	0.01221418979820834
5	0.0073579456615712885
6	0.0279601935139709
7	0.01272924599451833
8	0.012140610341592627
9	0.008093740227728418
10-11	0.007615473759726284
12-13	0.00610709489910417
14-15	0.005628828431102037
16-17	0.004341187940327061
18-19	0.00548166951787061
20-21	0.00496661332156062
22-23	0.004929823593252764
24-25	0.005334510604639185
26-27	0.004046870113864209
28-29	0.005739197616025605
30-31	0.005040192778176333
32-33	0.00776263267295771
34-35	0.007578684031418428
36-37	0.0068428894652612995
38-39	0.006953258650184868
40-41	0.0071372072917241505
42-43	0.006511781910490591
44-45	0.006769310008645587
46-47	0.008572006695730551
48-49	0.00787300185788128
50-51	0.005518459246178467
52-53	0.005224141419715615
54-55	0.005702407887717749
56-57	0.007026838106800582
58-59	0.006990048378492725
60-61	0.0045619263101742
62-63	0.005444879789562754
64-65	0.003200706362783511
66-67	0.003862921472324927
68-69	0.00610709489910417
70-71	0.004414767396942774
72-73	0.004341187940327061
74-75	0.0035318139175542188
76-77	0.004267608483711348
78-79	0.00279601935139709
80-81	0.003053547449552085
82-83	0.005224141419715615
84-85	0.008829534793885548
86-87	0.006474992182182734
88-89	0.004230818755403491
90-91	0.004819454408329194
92-93	0.007652263488034141
94-95	0.006879679193569155
96-97	0.008535216967422696
98-99	0.006806099736953442
100-101	0.007799422401265566
102-103	0.00496661332156062
104-105	0.004414767396942774
106-107	0.006769310008645587
108-109	0.0062910435406434515
110-111	0.007026838106800582
112-113	0.004929823593252764
114-115	0.0021705939701635304
116-117	0.0026120707098578077
118-119	0.15286132111914352
120-121	0.010117175284660523
122-123	0.0019130658720085353
124-125	0.0015083788606221143
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1359075.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.08434825602652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.12102396134934	52.626722986806094
2	9.741154145353201	12.485110293329017
3	3.1957470328580504	6.143920973755161
4	1.4870659609790882	3.811906116922666
5	0.8438807490650293	2.703977390481993
6	0.5669087257389142	2.179798572577968
7	0.3924839978783222	1.7606456843466383
8	0.28424033445300695	1.457228526519676
9	0.19945361865215758	1.1503669642766616
>10	1.1134960312608995	12.444790513165168
>50	0.0415587508833622	1.7763247861640918
>100	0.012641923164086601	1.299023836110214
>500	3.447683644829364E-4	0.16018335554459256
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.357945661571289E-5
2	0.0	0.0	0.0	0.0	7.357945661571289E-5
3	0.0	0.0	0.0	0.0	7.357945661571289E-5
4	0.0	0.0	0.0	0.0	7.357945661571289E-5
5	0.0	0.0	0.0	0.0	7.357945661571289E-5
6	0.0	0.0	0.0	0.0	7.357945661571289E-5
7	0.0	0.0	0.0	0.0	7.357945661571289E-5
8	0.0	0.0	0.0	0.0	7.357945661571289E-5
9	0.0	0.0	0.0	0.0	1.4715891323142577E-4
10-11	0.0	0.0	0.0	0.0	1.4715891323142577E-4
12-13	0.0	0.0	0.0	0.0	2.9431782646285155E-4
14-15	0.0	0.0	0.0	0.0	3.3110755477070797E-4
16-17	0.0	0.0	0.0	0.0	3.6789728307856445E-4
18-19	0.0	0.0	0.0	0.0	3.6789728307856445E-4
20-21	0.0	0.0	0.0	0.0	4.7826646800213377E-4
22-23	0.0	0.0	0.0	1.1036918492356932E-4	5.886356529257032E-4
24-25	0.0	0.0	0.0	5.886356529257032E-4	8.461637510806983E-4
26-27	0.0	0.0	0.0	0.0010301123926199805	8.829534793885547E-4
28-29	0.0	0.0	0.0	0.0050034030498684766	8.829534793885547E-4
30-31	0.0	0.0	0.0	0.01810054632746537	8.829534793885547E-4
32-33	0.0	0.0	0.0	0.04278645402203705	8.829534793885547E-4
34-35	0.0	0.0	0.0	0.07243897503816935	9.565329360042676E-4
36-37	0.0	0.0	0.0	0.11739602303036992	9.565329360042676E-4
38-39	0.0	0.0	0.0	0.19288854551809134	0.0010301123926199805
40-41	0.0	0.0	0.0	0.27805676655077904	0.0010301123926199805
42-43	0.0	0.0	0.0	0.3585894818166768	0.0010301123926199805
44-45	0.0	0.0	0.0	0.4465905119290694	0.001066902120927837
46-47	0.0	0.0	0.0	0.5351801776943877	0.0011036918492356934
48-49	0.0	0.0	0.0	0.6368669867373029	0.0011036918492356934
50-51	0.0	0.0	0.0	0.7407979692069975	0.0011036918492356934
52-53	0.0	3.678972830785644E-5	0.0	0.8487758217905561	0.0012508507624671194
54-55	0.0	7.357945661571289E-5	0.0	0.9663925831907731	0.0014347994040064013
56-57	0.0	7.357945661571289E-5	0.0	1.0910729724260988	0.0014715891323142578
58-59	0.0	7.357945661571289E-5	0.0	1.224950793738388	0.0014715891323142578
60-61	0.0	7.357945661571289E-5	0.0	1.3628386954362341	0.0014715891323142578
62-63	0.0	7.357945661571289E-5	0.0	1.5183856667218514	0.0016187480455456837
64-65	0.0	7.357945661571289E-5	0.0	1.6741533763773155	0.0016187480455456837
66-67	0.0	7.357945661571289E-5	0.0	1.8449680849106929	0.0016187480455456837
68-69	0.0	7.357945661571289E-5	0.0	2.0360171440133916	0.0016187480455456837
70-71	0.0	7.357945661571289E-5	0.0	2.242149991722311	0.0017659069587771094
72-73	0.0	7.357945661571289E-5	0.0	2.4333462097382412	0.0020970145135478175
74-75	0.0	7.357945661571289E-5	0.0	2.63554255651822	0.002133804241855674
76-77	0.0	7.357945661571289E-5	0.0	2.871327925243272	0.002133804241855674
78-79	0.0	7.357945661571289E-5	0.0	3.1344112723727537	0.002133804241855674
80-81	0.0	7.357945661571289E-5	0.0	3.3919025807994405	0.002133804241855674
82-83	0.0	1.4715891323142577E-4	0.0	3.6612033920129496	0.002133804241855674
84-85	0.0	1.4715891323142577E-4	0.0	3.9446682486249838	0.002133804241855674
86-87	0.0	1.4715891323142577E-4	0.0	4.253297279399591	0.0021705939701635304
88-89	0.0	1.4715891323142577E-4	0.0	4.598936776851903	0.0022809631550871
90-91	0.0	1.4715891323142577E-4	0.0	4.968121700421243	0.0022809631550871
92-93	0.0	1.4715891323142577E-4	0.0	5.336386880782886	0.0022809631550871
94-95	0.0	1.4715891323142577E-4	0.0	5.7040634254916025	0.0023545426117028124
96-97	0.0	1.4715891323142577E-4	0.0	6.098927579419826	0.0024281220683185253
98-99	0.0	1.4715891323142577E-4	0.0	6.549344223092913	0.0025017015249342383
100-101	0.0	1.4715891323142577E-4	0.0	7.042731269429575	0.0025752809815499512
102-103	0.0	1.4715891323142577E-4	0.0	7.544028107352427	0.002648860438165664
104-105	7.357945661571289E-5	1.4715891323142577E-4	0.0	8.061181318175965	0.002648860438165664
106-107	7.357945661571289E-5	1.4715891323142577E-4	0.0	8.610893438551956	0.0026856501664735207
108-109	7.357945661571289E-5	1.4715891323142577E-4	0.0	9.18970623401946	0.0028695988080128026
110-111	7.357945661571289E-5	1.4715891323142577E-4	0.0	9.815646671449331	0.0030167577212442285
112-113	7.357945661571289E-5	1.4715891323142577E-4	0.0	10.439894781377038	0.0030167577212442285
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1220	0.0	59.997707	1
GTATCAA	2085	0.0	51.66089	1
GTACATG	7040	0.0	42.011974	1
TCAACGC	2545	0.0	41.152695	4
CAACGCA	2655	0.0	39.44623	5
TACATGG	7365	0.0	39.187035	2
ACATGGG	7305	0.0	38.694283	3
AACGCAG	2870	0.0	36.488514	6
TATCAAC	2955	0.0	36.248363	2
CATGGGG	6660	0.0	35.74038	4
ATCAACG	2965	0.0	35.524006	3
ATGGGGG	4255	0.0	35.101974	5
TGGGGGG	3070	0.0	30.041304	6
GAGTACA	4745	0.0	28.845694	1
ACGCAGA	3630	0.0	28.844799	7
AGTACAT	4705	0.0	28.45747	2
CGCAGAG	3820	0.0	27.25337	8
GTATAGG	460	0.0	27.167572	1
GCAGAGT	4375	0.0	23.252174	9
AGAGTAC	3895	0.0	21.541159	5
>>END_MODULE