FastQCFastQC Report
Mon 15 Aug 2016
SRR2921310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359075
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.16857053510659822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.13259018082151464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15730.11574048525651638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18100.067.684351
GTATCAA33450.053.2393951
GTACATG66100.040.32241
TCAACGC42750.039.7300954
CAACGCA43050.039.1763655
TATCAAC44900.038.6240232
ATCAACG44200.038.426733
GTGGTAT10350.038.1080361
TACATGG68500.037.932092
AACGCAG45050.037.6878136
ACATGGG68950.037.3387953
TGGTATC10100.037.1732062
CATGGGG62550.035.061574
ATGGGGG39100.034.1414645
TATACCG1254.8303504E-528.605675
ACGCAGA58750.028.488487
CGCAGAG60400.027.3117628
TACGGCG1401.0485856E-425.5266637
TGGGGGG30000.025.0207376
AGAGTAC61400.023.8768835
Produced by FastQC (version 0.11.5)