FastQCFastQC Report
Mon 15 Aug 2016
SRR2921309_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921309_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038764
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC12380.11918010250644034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.053.7782551
GTATCAA20350.044.4457321
GTACATG62850.041.847341
TACATGG63350.040.8556142
ACATGGG66650.038.564943
ATGGGGG35750.037.946165
CATGGGG55500.037.8434564
TCAACGC24850.034.7176784
TATCAAC26700.033.64922
CAACGCA25750.033.5042465
TGGGGGG29350.032.63516
ATCAACG27400.032.13813
AACGCAG27200.031.9338446
GTATAGG2654.7293724E-1126.94551
ACGCAGA32650.026.2364277
GAGTACA42200.026.086161
AGTACAT42000.026.066192
CGCAGAG34600.024.7577888
CCTATAC2302.3636494E-723.2821853
CCCTATA1903.3056996E-521.9206522
Produced by FastQC (version 0.11.5)