##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921309_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038764
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.630934456719718	31.0	31.0	34.0	25.0	34.0
2	29.713459457586133	31.0	31.0	34.0	25.0	34.0
3	29.94039550850819	31.0	31.0	34.0	25.0	34.0
4	33.24595577051188	37.0	35.0	37.0	27.0	37.0
5	33.3467447851485	37.0	35.0	37.0	27.0	37.0
6	33.39553353793547	37.0	35.0	37.0	27.0	37.0
7	33.31561259342834	37.0	35.0	37.0	27.0	37.0
8	33.318843356142494	37.0	35.0	37.0	27.0	37.0
9	34.8162749190384	39.0	37.0	39.0	27.0	39.0
10-11	34.80574798510538	39.0	37.0	39.0	27.0	39.0
12-13	34.71172903566161	39.0	37.0	39.0	26.0	39.0
14-15	35.914372754542896	40.0	37.0	41.0	25.5	41.0
16-17	35.882524808329904	40.0	37.0	41.0	25.0	41.0
18-19	35.88849729101124	40.0	37.0	41.0	25.0	41.0
20-21	35.92250116484591	40.0	37.0	41.0	25.0	41.0
22-23	35.87742451605947	40.0	37.0	41.0	25.0	41.0
24-25	35.86777458595023	40.0	37.0	41.0	25.0	41.0
26-27	35.84390342753503	40.0	36.5	41.0	26.0	41.0
28-29	35.76673527384469	40.0	36.0	41.0	26.0	41.0
30-31	35.67734297684556	40.0	36.0	41.0	25.0	41.0
32-33	35.57558983561232	40.0	36.0	41.0	25.0	41.0
34-35	35.45325742902142	40.0	36.0	41.0	24.5	41.0
36-37	35.33689846779441	40.0	35.0	41.0	24.0	41.0
38-39	35.22407207026812	40.0	35.0	41.0	23.5	41.0
40-41	35.10913162181208	40.0	35.0	41.0	23.0	41.0
42-43	34.90498082336315	40.0	35.0	41.0	21.5	41.0
44-45	34.659829374140806	40.0	35.0	41.0	19.5	41.0
46-47	34.42120587544428	39.0	35.0	41.0	18.5	41.0
48-49	34.205995298258316	39.0	34.0	41.0	15.5	41.0
50-51	34.02320931414643	39.0	34.0	41.0	14.5	41.0
52-53	33.82266039254345	38.5	34.0	40.5	12.0	41.0
54-55	33.61295491564975	38.0	34.0	40.0	8.5	41.0
56-57	33.36749829605185	38.0	33.0	40.0	8.0	41.0
58-59	33.09860468787906	37.5	33.0	40.0	6.5	41.0
60-61	32.80505052158142	37.0	33.0	40.0	2.0	41.0
62-63	32.464815395989845	36.5	32.0	40.0	2.0	41.0
64-65	32.12688685784259	36.0	32.0	39.0	2.0	41.0
66-67	31.793849228506186	35.5	32.0	39.0	2.0	40.5
68-69	31.45194481133347	35.0	31.0	38.5	2.0	40.0
70-71	31.409460185374158	35.0	32.0	38.0	2.0	40.0
72-73	31.12046287703463	35.0	32.0	37.0	2.0	39.5
74-75	30.79643258719016	35.0	31.0	37.0	2.0	39.0
76-77	30.4985656029666	35.0	31.0	36.0	2.0	39.0
78-79	30.214717683708713	35.0	31.0	36.0	2.0	37.5
80-81	29.940838342491652	35.0	31.0	35.0	2.0	37.0
82-83	29.721128186960655	35.0	31.0	35.0	2.0	36.5
84-85	29.50387046528374	35.0	31.0	35.0	2.0	36.0
86-87	29.32691256146728	35.0	30.0	35.0	2.0	36.0
88-89	29.181812712030833	35.0	30.0	35.0	2.0	35.5
90-91	29.05421202506055	34.0	30.0	35.0	2.0	35.0
92-93	28.933551798098506	34.0	30.0	35.0	2.0	35.0
94-95	28.80258990492547	34.0	30.0	35.0	2.0	35.0
96-97	28.709113427111454	34.0	30.0	35.0	2.0	35.0
98-99	28.61897456977716	34.0	29.5	35.0	2.0	35.0
100-101	28.52667641543219	34.0	29.0	35.0	2.0	35.0
102-103	27.702916639390658	33.5	28.0	34.5	2.0	35.0
104-105	28.289237497641427	34.0	29.0	35.0	2.0	35.0
106-107	28.360815834973103	34.0	29.0	35.0	2.0	35.0
108-109	28.292036978562983	34.0	29.0	35.0	2.0	35.0
110-111	28.214741269431748	34.0	29.0	35.0	2.0	35.0
112-113	28.114550562014088	34.0	29.0	35.0	2.0	35.0
114-115	27.981839955947645	34.0	29.0	35.0	2.0	35.0
116-117	27.84248154537508	34.0	29.0	35.0	2.0	35.0
118-119	27.83281188027309	34.0	29.0	35.0	2.0	35.0
120-121	27.997975959890795	34.0	29.0	35.0	2.0	35.0
122-123	27.856629609805502	34.0	29.0	35.0	2.0	35.0
124-125	27.262951931333777	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50061.0
3	7221.0
4	4501.0
5	3697.0
6	3710.0
7	4291.0
8	4511.0
9	4578.0
10	5282.0
11	5756.0
12	5707.0
13	5776.0
14	5999.0
15	5240.0
16	4356.0
17	3935.0
18	3640.0
19	3439.0
20	3395.0
21	3362.0
22	3819.0
23	5021.0
24	7318.0
25	8913.0
26	9444.0
27	10478.0
28	12370.0
29	14705.0
30	18039.0
31	22144.0
32	28641.0
33	39433.0
34	61742.0
35	97715.0
36	192344.0
37	296514.0
38	71620.0
39	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.462764687305025	17.622070049220863	12.127569845096787	24.78759541837733
2	16.14653035995217	19.4072429470133	39.33718966569681	25.109037027337727
3	19.61249288732659	24.85146427199441	28.438080988678454	27.09796185200055
4	13.60334754460761	15.145405599010989	34.86229698867335	36.38894986770806
5	14.246777128443105	38.691499706355245	32.16343978356937	14.898283381632279
6	33.45113858616363	34.01044069115411	17.47607221237302	15.062348510309242
7	29.53583085410589	32.933961083350184	19.40604450092911	18.12416356161482
8	27.72483420499272	35.561392292939175	18.996815041323586	17.716958460744518
9	28.718031170691695	16.28297011172001	17.939899948780553	37.05909876880774
10-11	26.70370741212852	26.04825758946967	26.234408034844357	21.013626963557453
12-13	25.958035521735113	23.605307849902953	26.59041983733325	23.846236791028684
14-15	23.895293311202938	24.746411833344244	23.9867544950765	27.37154036037632
16-17	24.12693599046897	27.619865939805287	24.516167852028353	23.737030217697388
18-19	24.163699405938114	27.099686963420016	25.804554073302448	22.932059557339414
20-21	25.148743621834985	25.995523250216618	25.542119957639358	23.31361317030904
22-23	25.013658893973766	25.21862654345502	25.663172396662947	24.104542165908267
24-25	24.443005680177144	25.205786078752286	25.69505150669106	24.656156734379515
26-27	23.95565071641817	25.690448431658915	25.84626685626099	24.507633995661923
28-29	24.195157878015493	26.5469975618503	25.48128786292448	23.776556697209728
30-31	25.33273515057021	25.697036155238056	25.529471171457825	23.44075752273391
32-33	24.845801363562188	25.560701527376427	25.53412925006005	24.059367859001334
34-35	24.58362978360013	26.03494438609041	25.714392993407976	23.667032836901488
36-37	24.45420144423066	26.04977738668479	25.743957862084205	23.752063307000345
38-39	24.53503932838479	26.213499696733383	25.46269820639459	23.788762768487228
40-41	24.620109513207776	25.791443528491488	25.710090859859196	23.878356098441543
42-43	24.233718596109505	25.8998156322658	25.367989332665825	24.49847643895888
44-45	23.81076851462738	26.037621589980798	25.92979299316299	24.221816902228827
46-47	23.786169643807025	25.654385992782075	25.751241137056287	24.80820322635461
48-49	23.91981526490683	25.99736299030545	25.7075229871861	24.37529875760162
50-51	23.942790081072417	26.120413863084757	25.834431660314184	24.102364395528646
52-53	24.17971215132682	26.026337590394967	25.584056308549442	24.209893949728773
54-55	23.736549809929492	25.557298664139132	26.172157619162917	24.533993906768455
56-57	24.064102391299745	25.848238440639193	25.504966871477507	24.582692296583556
58-59	24.03795748195922	26.08197741671348	25.776303482709206	24.103761618618094
60-61	24.660779201774517	25.96191985397187	25.74997304333092	23.627327900922687
62-63	24.35120824107057	26.118272841051315	25.617887744295754	23.912631173582362
64-65	24.14336876155268	25.66918900184843	25.63544554836722	24.551996688231668
66-67	24.075340767800384	25.91062754610119	25.519136283533445	24.494895402564982
68-69	23.632657933905463	25.778622000319633	25.95851136138266	24.630208704392246
70-71	23.784187871675318	25.650109896034408	26.187700308748646	24.37800192354163
72-73	23.86875905866428	26.284627503873775	25.49427634419627	24.35233709326567
74-75	23.773556750407955	26.18023230626303	25.6655579249361	24.380653018392916
76-77	23.624603414140335	25.496252802154984	25.86988951193765	25.009254271767034
78-79	23.567911907640884	25.632985728698948	26.137593287174315	24.661509076485856
80-81	23.934456531313945	25.89396383373743	25.630324708863366	24.541254926085255
82-83	24.096110060122943	25.98220861553569	25.793752797955143	24.12792852638622
84-85	24.05062072438143	26.20636769007924	25.416074761316327	24.326936824223004
86-87	23.499017027282548	26.535401459151274	25.578663811777574	24.386917701788605
88-89	23.348550941601545	25.880492033861263	25.902057265316852	24.86889975922034
90-91	24.038792478914882	25.486891629156073	25.97567262744577	24.498643264483277
92-93	24.086688916070838	25.74658496677753	25.789138998122134	24.3775871190295
94-95	23.961959703009953	25.81468159832404	25.608074802057978	24.615283896608027
96-97	23.461635479286933	25.6696076128272	25.890708099219385	24.978048808666482
98-99	23.248600040146705	25.801237230917195	25.69100227642402	25.25916045251208
100-101	23.29525925769031	26.04178199767589	25.851780678690297	24.811178065943505
102-103	23.064414886242858	25.913349712718926	25.993930898504292	25.028304502533928
104-105	23.433871694451184	25.964708079652798	25.76754005938146	24.833880166514554
106-107	23.460385006041292	25.774849930440897	25.98819661398788	24.776568449529933
108-109	23.292190222024537	25.858881101847853	26.00777035337249	24.84115832275512
110-111	23.058486223626996	26.418229667351987	25.961400064793743	24.561884044227266
112-113	23.051851152975246	26.45359024322617	25.907140051448728	24.587418552349853
114-115	23.171012888241734	26.627660982683306	25.70779431762115	24.49353181145381
116-117	23.000090976612274	26.405985779729736	25.814397121095645	24.779526122562345
118-119	22.93483768602118	26.064732742282537	26.103350803867976	24.897078767828308
120-121	22.942103294512165	26.1257505525193	26.322303486780697	24.609842666187838
122-123	22.806065816340777	26.341265707747567	26.00836206207719	24.844306413834467
124-125	22.595652364359612	26.23873825088556	25.879170944651182	25.28643844010364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	483.0
1	375.0
2	228.5
3	192.0
4	174.5
5	149.5
6	146.0
7	183.5
8	241.0
9	296.0
10	349.0
11	374.0
12	397.5
13	439.5
14	458.5
15	472.0
16	537.0
17	597.5
18	620.0
19	643.0
20	723.0
21	834.0
22	929.0
23	1060.0
24	1295.5
25	1672.5
26	2306.5
27	3018.5
28	3573.0
29	4436.0
30	5760.0
31	7120.5
32	8661.5
33	10252.5
34	12005.5
35	13921.5
36	15830.5
37	17805.5
38	19327.0
39	21040.0
40	23081.0
41	24730.0
42	26382.5
43	29006.0
44	31973.5
45	34536.0
46	37086.5
47	39937.0
48	43475.0
49	46460.0
50	48464.5
51	49711.0
52	48972.0
53	47832.5
54	46721.0
55	44490.5
56	41560.0
57	37751.0
58	33847.0
59	29337.5
60	24442.0
61	20689.0
62	17185.5
63	13137.0
64	9610.0
65	7224.5
66	5433.0
67	4191.0
68	3258.0
69	2424.0
70	1849.0
71	1395.0
72	1038.5
73	806.0
74	623.0
75	439.5
76	296.5
77	196.5
78	125.5
79	91.5
80	62.5
81	41.0
82	31.5
83	26.0
84	19.0
85	15.5
86	15.5
87	13.0
88	9.5
89	6.0
90	6.0
91	5.5
92	2.5
93	0.5
94	2.0
95	2.5
96	1.0
97	1.5
98	1.5
99	0.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0173282863094986
2	0.012129800416649019
3	0.012033532159374026
4	0.014632775105798815
5	0.009049216183849266
6	0.03147972012892245
7	0.012899946474848956
8	0.01270740996029897
9	0.009241752698399252
10-11	0.008471606640199313
12-13	0.007316387552899407
14-15	0.006931314523799438
16-17	0.004957815249662098
18-19	0.006498107366061974
20-21	0.0061611684655995015
22-23	0.006113034336962004
24-25	0.0061611684655995015
26-27	0.005294754150124571
28-29	0.006401839108786982
30-31	0.006016766079687013
32-33	0.008423472511561819
34-35	0.008182801868374338
36-37	0.0076533264533618795
38-39	0.007123851038349423
40-41	0.007364521681536903
42-43	0.007605192324724384
44-45	0.007557058196086888
46-47	0.009578691598861724
48-49	0.008423472511561819
50-51	0.006064900208324509
52-53	0.005631693050587043
54-55	0.00678691213788695
56-57	0.00789399709654936
58-59	0.007268253424261912
60-61	0.005776095436499532
62-63	0.0061611684655995015
64-65	0.004235803320099657
66-67	0.004187669191462161
68-69	0.0067387780092494545
70-71	0.005294754150124571
72-73	0.004669010477837122
74-75	0.004717144606474618
76-77	0.005246620021487076
78-79	0.003465657261899719
80-81	0.003224986618712239
82-83	0.00567982717922454
84-85	0.009482423341586731
86-87	0.007508924067449393
88-89	0.005294754150124571
90-91	0.005535424793312052
92-93	0.008134667739736841
94-95	0.007316387552899407
96-97	0.009241752698399252
98-99	0.007075716909711927
100-101	0.008375338382924322
102-103	0.005776095436499532
104-105	0.005391022407399563
106-107	0.007605192324724384
108-109	0.006979448652436935
110-111	0.00803839948246185
112-113	0.005776095436499532
114-115	0.002551108817787293
116-117	0.0034175231332622233
118-119	0.16216387937972435
120-121	0.010830178943436623
122-123	0.002406706431874805
124-125	0.001492157987762379
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1038764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.53885940340724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0517307408799	57.6080975586221
2	8.91518256218802	12.220728883710253
3	2.8179418045178357	5.794155514404949
4	1.3521796362394232	3.7070739990545665
5	0.7674698497058384	2.630075406267127
6	0.48243951767774057	1.9839512563657358
7	0.3155038979056021	1.5136994109845312
8	0.23783650544370238	1.3040834246082893
9	0.17969247665869376	1.1084325654204226
>10	0.8461317593689801	9.728319545894449
>50	0.025880795887408416	1.1931375763025474
>100	0.007448316979318582	0.8950106102712135
>500	4.216024106566431E-4	0.1939894759104677
>1k	1.405341368855477E-4	0.11924477218329642
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1238	0.11918010250644034	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.62682572749922E-5
7	0.0	0.0	0.0	0.0	9.62682572749922E-5
8	0.0	0.0	0.0	0.0	9.62682572749922E-5
9	0.0	0.0	0.0	0.0	9.62682572749922E-5
10-11	0.0	0.0	0.0	0.0	9.62682572749922E-5
12-13	0.0	0.0	0.0	0.0	9.62682572749922E-5
14-15	0.0	0.0	0.0	0.0	9.62682572749922E-5
16-17	0.0	0.0	0.0	9.62682572749922E-5	9.62682572749922E-5
18-19	0.0	0.0	0.0	9.62682572749922E-5	9.62682572749922E-5
20-21	0.0	0.0	0.0	1.925365145499844E-4	9.62682572749922E-5
22-23	0.0	0.0	0.0	3.369389004624727E-4	9.62682572749922E-5
24-25	0.0	0.0	0.0	8.182801868374338E-4	9.62682572749922E-5
26-27	0.0	0.0	0.0	0.0011552190872999063	9.62682572749922E-5
28-29	0.0	0.0	0.0	0.0033693890046247272	9.62682572749922E-5
30-31	0.0	0.0	0.0	0.01333315363258642	1.444023859124883E-4
32-33	0.0	0.0	0.0	0.03720768143678449	1.925365145499844E-4
34-35	0.0	0.0	0.0	0.06796538963614449	1.925365145499844E-4
36-37	0.0	0.0	0.0	0.10762791163344129	1.925365145499844E-4
38-39	0.0	0.0	0.0	0.18006977523287293	1.925365145499844E-4
40-41	0.0	0.0	0.0	0.271861558544578	1.925365145499844E-4
42-43	0.0	0.0	0.0	0.3530638335560339	2.8880477182497663E-4
44-45	0.0	0.0	0.0	0.4311373902060526	2.8880477182497663E-4
46-47	0.0	0.0	0.0	0.5141687621057334	2.8880477182497663E-4
48-49	0.0	0.0	0.0	0.6089929955216007	2.8880477182497663E-4
50-51	0.0	0.0	0.0	0.7140697983372546	2.8880477182497663E-4
52-53	0.0	0.0	0.0	0.827618207793108	2.8880477182497663E-4
54-55	0.0	0.0	0.0	0.9479053952582107	3.369389004624727E-4
56-57	0.0	0.0	0.0	1.0671336318932885	3.850730290999688E-4
58-59	0.0	0.0	0.0	1.2007058388623402	3.850730290999688E-4
60-61	0.0	0.0	0.0	1.343904871558891	3.850730290999688E-4
62-63	0.0	0.0	0.0	1.5030363008344532	4.81341286374961E-4
64-65	0.0	0.0	0.0	1.647871893904679	4.81341286374961E-4
66-67	0.0	0.0	0.0	1.8119130043012657	4.81341286374961E-4
68-69	0.0	0.0	0.0	2.0019465441621005	4.81341286374961E-4
70-71	0.0	0.0	0.0	2.199152069189922	5.294754150124571E-4
72-73	9.62682572749922E-5	0.0	0.0	2.3831207088424318	7.701460581999376E-4
74-75	9.62682572749922E-5	0.0	0.0	2.5732986510891793	7.701460581999376E-4
76-77	9.62682572749922E-5	0.0	0.0	2.8022245668891106	7.701460581999376E-4
78-79	9.62682572749922E-5	0.0	0.0	3.0493451833140153	7.701460581999376E-4
80-81	9.62682572749922E-5	0.0	0.0	3.2797632571017097	7.701460581999376E-4
82-83	9.62682572749922E-5	0.0	0.0	3.526354398111602	7.701460581999376E-4
84-85	9.62682572749922E-5	0.0	0.0	3.7904182278169056	7.701460581999376E-4
86-87	9.62682572749922E-5	0.0	0.0	4.075901744765895	8.182801868374338E-4
88-89	9.62682572749922E-5	0.0	0.0	4.403598892529969	8.664143154749298E-4
90-91	9.62682572749922E-5	0.0	0.0	4.742366889880666	8.664143154749298E-4
92-93	9.62682572749922E-5	0.0	0.0	5.046863387641466	8.664143154749298E-4
94-95	9.62682572749922E-5	0.0	0.0	5.359350150756091	9.62682572749922E-4
96-97	9.62682572749922E-5	0.0	0.0	5.719730371865024	0.001010816701387418
98-99	9.62682572749922E-5	0.0	0.0	6.142973764974528	0.0010589508300249142
100-101	9.62682572749922E-5	0.0	0.0	6.576999202898829	0.0010589508300249142
102-103	9.62682572749922E-5	0.0	0.0	7.041012202964293	0.0010589508300249142
104-105	9.62682572749922E-5	0.0	0.0	7.511523310395816	0.0010589508300249142
106-107	9.62682572749922E-5	0.0	0.0	8.007208567104751	0.0010589508300249142
108-109	9.62682572749922E-5	0.0	0.0	8.522003072882772	0.0010589508300249142
110-111	9.62682572749922E-5	0.0	0.0	9.098890604603163	0.0010589508300249142
112-113	9.62682572749922E-5	0.0	0.0	9.649448767958843	0.0010589508300249142
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1195	0.0	53.778255	1
GTATCAA	2035	0.0	44.445732	1
GTACATG	6285	0.0	41.84734	1
TACATGG	6335	0.0	40.855614	2
ACATGGG	6665	0.0	38.56494	3
ATGGGGG	3575	0.0	37.94616	5
CATGGGG	5550	0.0	37.843456	4
TCAACGC	2485	0.0	34.717678	4
TATCAAC	2670	0.0	33.6492	2
CAACGCA	2575	0.0	33.504246	5
TGGGGGG	2935	0.0	32.6351	6
ATCAACG	2740	0.0	32.1381	3
AACGCAG	2720	0.0	31.933844	6
GTATAGG	265	4.7293724E-11	26.9455	1
ACGCAGA	3265	0.0	26.236427	7
GAGTACA	4220	0.0	26.08616	1
AGTACAT	4200	0.0	26.06619	2
CGCAGAG	3460	0.0	24.757788	8
CCTATAC	230	2.3636494E-7	23.282185	3
CCCTATA	190	3.3056996E-5	21.920652	2
>>END_MODULE