FastQCFastQC Report
Mon 15 Aug 2016
SRR2921309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038764
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.20351109587933353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.1654851342557116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16220.15614711330003736No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC12070.11619578653091558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.063.8186451
GTATCAA30550.050.865251
GTACATG62450.039.5254361
TACATGG64400.038.3102762
ACATGGG68650.035.9385533
CATGGGG57450.035.8911064
TCAACGC42900.035.145044
ATGGGGG39550.034.8069765
TATCAAC44800.034.5856632
ATCAACG44500.034.149233
CAACGCA44350.033.7272455
AACGCAG46100.032.6912846
TGGGGGG32900.030.7797746
GTATACG800.00524601129.8833351
ACGCAGA55300.026.7075637
TAACGCA900.00945568726.4861164
CGCAGAG58050.025.5437058
TATAGGG1401.0445865E-425.5401822
AGAGTAC58600.024.3385510-11
GTGGTAT8900.024.1752821
Produced by FastQC (version 0.11.5)