##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1038764
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.90827271642067	31.0	30.0	34.0	16.0	34.0
2	29.719404985155435	33.0	31.0	34.0	25.0	34.0
3	29.96063109618739	33.0	31.0	34.0	26.0	34.0
4	31.99364629501985	37.0	33.0	37.0	19.0	37.0
5	31.669015291249984	37.0	33.0	37.0	19.0	37.0
6	31.828597255969594	37.0	33.0	37.0	17.0	37.0
7	32.06076548667455	37.0	35.0	37.0	17.0	37.0
8	32.3242728858528	37.0	35.0	37.0	17.0	37.0
9	34.03172712955012	39.0	35.0	39.0	17.0	39.0
10-11	34.2408574036066	39.0	37.0	39.0	17.0	39.0
12-13	34.27419365707706	39.0	37.0	39.0	17.5	39.0
14-15	35.64176415432186	40.0	37.0	41.0	19.0	41.0
16-17	35.68037157621943	40.0	37.5	41.0	21.5	41.0
18-19	35.710943005340965	40.5	38.0	41.0	21.5	41.0
20-21	35.707935103642406	41.0	38.0	41.0	21.5	41.0
22-23	35.664352056867585	40.5	37.5	41.0	21.5	41.0
24-25	35.607558117146915	40.5	37.0	41.0	19.0	41.0
26-27	35.5104638782245	40.0	37.0	41.0	18.5	41.0
28-29	35.42615358252692	40.0	37.0	41.0	18.0	41.0
30-31	35.30690031614496	40.0	36.5	41.0	17.0	41.0
32-33	35.14751666403534	40.0	36.0	41.0	16.0	41.0
34-35	35.06170217681783	40.0	35.5	41.0	15.0	41.0
36-37	34.979912665437	40.0	35.0	41.0	11.5	41.0
38-39	34.87237765267183	40.0	35.0	41.0	9.0	41.0
40-41	34.724589030809696	40.0	35.0	41.0	8.0	41.0
42-43	34.56729247451779	40.0	35.0	41.0	2.0	41.0
44-45	34.39462187753907	40.0	35.0	41.0	2.0	41.0
46-47	34.1426637811861	40.0	35.0	41.0	2.0	41.0
48-49	33.89274560920478	40.0	35.0	41.0	2.0	41.0
50-51	33.69069682815346	39.5	35.0	41.0	2.0	41.0
52-53	33.51258900000386	39.0	34.5	41.0	2.0	41.0
54-55	33.31748212298463	39.0	34.0	41.0	2.0	41.0
56-57	33.08145016577394	38.5	34.0	41.0	2.0	41.0
58-59	33.01651963294839	38.5	34.0	41.0	2.0	41.0
60-61	32.77583551220489	37.5	34.0	41.0	2.0	41.0
62-63	32.4892425035908	37.0	34.0	40.0	2.0	41.0
64-65	32.19499857522979	36.5	33.5	40.0	2.0	41.0
66-67	31.883410957638116	36.0	33.0	39.0	2.0	41.0
68-69	31.554186513972375	35.5	33.0	39.0	2.0	41.0
70-71	31.227052054172074	35.0	33.0	38.5	2.0	40.5
72-73	30.89041495469616	35.0	33.0	37.0	2.0	39.5
74-75	30.556702966217543	35.0	32.0	37.0	2.0	39.0
76-77	30.265359600448228	35.0	32.0	36.0	2.0	39.0
78-79	30.01054907563219	35.0	32.0	36.0	2.0	37.5
80-81	29.784385577474765	35.0	32.0	35.5	2.0	37.0
82-83	29.58527682900062	35.0	32.0	35.0	2.0	37.0
84-85	29.415325328948633	35.0	31.5	35.0	2.0	36.0
86-87	29.27188514426761	35.0	31.0	35.0	2.0	36.0
88-89	29.14759271595858	35.0	31.0	35.0	2.0	36.0
90-91	29.04961040236281	35.0	31.0	35.0	2.0	35.5
92-93	28.978917732998063	35.0	31.0	35.0	2.0	35.0
94-95	28.907496794267033	35.0	31.0	35.0	2.0	35.0
96-97	28.842428116492293	35.0	31.0	35.0	2.0	35.0
98-99	28.789318844318824	35.0	31.0	35.0	2.0	35.0
100-101	28.73428420699986	35.0	31.0	35.0	2.0	35.0
102-103	28.074395146539544	34.0	29.5	35.0	2.0	35.0
104-105	28.599421524042036	35.0	30.5	35.0	2.0	35.0
106-107	28.649025187626833	35.0	31.0	35.0	2.0	35.0
108-109	28.714167029277103	35.0	31.0	35.0	2.0	35.0
110-111	28.82157015452981	35.0	31.5	35.0	2.0	35.0
112-113	28.78152785425756	35.0	31.5	35.0	2.0	35.0
114-115	28.711945639240483	35.0	31.0	35.0	2.0	35.0
116-117	28.638084781528818	35.0	31.0	35.0	2.0	35.0
118-119	28.55456292285832	35.0	31.0	35.0	2.0	35.0
120-121	28.452345287283734	35.0	31.0	35.0	2.0	35.0
122-123	28.337180052446946	35.0	31.0	35.0	2.0	35.0
124-125	27.822592523422067	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76812.0
3	4393.0
4	3313.0
5	3424.0
6	3346.0
7	3687.0
8	3694.0
9	3751.0
10	3972.0
11	4118.0
12	4746.0
13	6050.0
14	7716.0
15	6478.0
16	4012.0
17	3519.0
18	3066.0
19	2720.0
20	2493.0
21	2538.0
22	2886.0
23	3547.0
24	4705.0
25	6171.0
26	7025.0
27	7934.0
28	9209.0
29	11190.0
30	13553.0
31	16975.0
32	21507.0
33	28937.0
34	44731.0
35	75493.0
36	159399.0
37	347216.0
38	124173.0
39	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44869893683311	18.042922767035545	12.052380653357355	26.455997642773994
2	16.059181873842373	19.897974900939964	38.48265823613448	25.56018498908318
3	19.100199852902104	24.212525655490563	28.89896068789446	27.78831380371287
4	13.922284552234926	16.234432742321562	30.95262854842965	38.89065415701386
5	14.122788235816063	38.514547057314424	31.63538899851665	15.727275708352865
6	33.42502643882086	34.46273811794122	16.670037736721355	15.442197706516566
7	29.9858643089207	32.629180765445746	18.636772440409587	18.748182485223968
8	28.372067050939037	35.81358136776445	17.495111550988618	18.319240030307903
9	28.938470573510866	16.186638939433326	17.489915181623005	37.3849753054328
10-11	27.40403614965282	25.80315045909096	25.0985932442027	21.694220147053514
12-13	26.72816741792689	23.442642137005013	26.05716256262977	23.772027882438334
14-15	24.596732270275055	24.395868551470787	23.89517734538355	27.112221832870603
16-17	24.16294750299394	27.266154776253316	24.44905676361522	24.121840957137522
18-19	24.193657077064664	26.275602543022288	26.384193137228472	23.146547242684576
20-21	25.122790162154253	25.290345063941377	26.400366204450677	23.186498569453697
22-23	24.808426168022766	24.556973480020485	26.404938946671237	24.229661405285512
24-25	24.284823116704082	24.849821518651012	26.354542513987777	24.510812850657125
26-27	23.770173013311975	25.158265014960087	26.68902657388974	24.3825353978382
28-29	23.984369885748833	25.800566827498834	26.372544678098205	23.84251860865413
30-31	25.051166578741658	25.1688063891317	26.35049924718223	23.429527784944415
32-33	24.60530014517253	25.185508931768908	26.43011309594865	23.779077827109912
34-35	24.420128152304084	25.618812357763648	26.510930297931	23.450129192001263
36-37	24.41117520437751	25.480908079217222	26.601711264541322	23.506205451863945
38-39	24.281646264214007	25.70439483848112	26.35357983141503	23.66037906588985
40-41	24.289973468468297	25.4594402578449	26.438632836717485	23.811953436969322
42-43	24.08569222653076	25.465505158053226	26.116759918518547	24.332042696897467
44-45	23.60107781940845	25.704057899580658	26.60782429887828	24.08703998213261
46-47	23.623508323353523	25.58093079852594	26.03156251083018	24.76399836729036
48-49	23.943311474021048	25.932646876480124	25.713058981635868	24.41098266786296
50-51	23.907403414057477	26.022946501804068	25.93495731465472	24.13469276948373
52-53	24.095992930059186	25.859579269208403	25.740976776245617	24.303451024486794
54-55	23.66456673508131	25.43912765555988	26.277142835138683	24.61916277422013
56-57	23.955681945080883	25.761289378530638	25.70954519024533	24.573483486143147
58-59	23.89252996830849	25.79430939077596	26.01909577151307	24.294064869402483
60-61	24.502437512274202	25.661555463993746	26.124028171942808	23.711978851789244
62-63	24.227350967110915	25.72932831711534	25.97832616455711	24.064994551216635
64-65	23.905911256069714	25.345891848389044	26.036279655436655	24.711917240104587
66-67	23.878185997974516	25.638739887019575	25.820590625012034	24.66248348999388
68-69	23.507360670951247	25.610581421766636	26.143426225783724	24.738631681498397
70-71	23.653736556137872	25.551087638770692	26.26217312113242	24.533002683959012
72-73	23.680450997531683	26.2310784740326	25.561436476427758	24.527034052007963
74-75	23.68300210634947	26.072187715400226	25.68307141949471	24.561738758755595
76-77	23.541006426868854	25.402016242380366	25.84340620198621	25.213571128764574
78-79	23.42688040786935	25.653228259739457	26.10053871716771	24.81935261522348
80-81	23.746346619636412	25.81683616297831	25.670797216692147	24.766020000693132
82-83	23.80271168427092	25.912815615481477	25.77264903288909	24.511823667358517
84-85	23.716166520980703	26.123546830656437	25.360476489366206	24.799810158996653
86-87	23.322621885240537	26.32527696377618	25.530486231713844	24.82161491926944
88-89	23.097161626702505	25.935246119426548	25.80379181411755	25.1638004397534
90-91	23.691219118903504	25.564120768703148	25.8041660148494	24.940494097543954
92-93	23.70843726323957	25.82008758511342	25.641509452126137	24.829965699520873
94-95	23.678537029843653	25.839375372738836	25.391535224331623	25.090552373085885
96-97	23.189261052349867	25.61898261486521	25.633856103569542	25.55790022921538
98-99	23.036560758747896	25.684659845739745	25.517393748724444	25.761385646787915
100-101	23.092348213838754	26.07868582276629	25.52961981739837	25.299346145996587
102-103	22.895190822939572	25.830361853125446	25.672000569908082	25.602446754026904
104-105	23.20974735358561	25.934042766210613	25.373280167583783	25.48292971262
106-107	23.2505393434306	25.611568760150295	25.64993169760571	25.487960198813397
108-109	23.149068938860818	25.70297691191718	25.563531938530616	25.584422210691393
110-111	22.82327424376325	26.355507806408013	25.541413721333665	25.279804228495074
112-113	22.875625085137692	26.362816766360996	25.474352315769256	25.287205832732056
114-115	22.90592070175844	26.319647811529496	25.33982760247294	25.43460388423912
116-117	22.711138964860954	26.17560954947201	25.41133217618331	25.701919309483724
118-119	22.686947208818182	25.848690148132995	25.65856006546251	25.805802577586313
120-121	22.683577814948073	25.97783420175449	25.802914525697645	25.53567345759979
122-123	22.537891915621174	26.209922752863076	25.437162573261556	25.815022758254198
124-125	22.2556807898618	26.14371503055554	25.225171453766205	26.37543272581645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	42.0
2	66.5
3	71.5
4	54.0
5	84.5
6	173.0
7	432.5
8	656.0
9	735.0
10	727.0
11	657.5
12	675.5
13	749.5
14	824.5
15	882.0
16	908.5
17	982.0
18	1097.0
19	1144.0
20	1200.0
21	1292.0
22	1367.0
23	1482.0
24	1609.0
25	1879.0
26	2462.0
27	3110.0
28	3680.0
29	4364.0
30	5396.5
31	6687.0
32	8147.0
33	9589.5
34	11085.5
35	12682.0
36	14630.0
37	16752.0
38	18444.5
39	20118.0
40	22171.5
41	24028.5
42	25834.5
43	28289.0
44	30909.0
45	33388.0
46	35955.0
47	38924.5
48	42522.5
49	45900.0
50	48531.0
51	50143.0
52	49814.5
53	48665.5
54	47460.5
55	45176.5
56	41985.0
57	38366.5
58	34712.0
59	30264.0
60	25228.5
61	21121.0
62	17322.0
63	13314.5
64	9723.5
65	7254.0
66	5515.5
67	4215.0
68	3313.5
69	2527.0
70	1938.0
71	1481.0
72	1128.0
73	928.0
74	726.0
75	535.0
76	427.0
77	309.5
78	214.5
79	163.0
80	133.5
81	117.0
82	108.0
83	102.5
84	96.5
85	87.5
86	82.0
87	81.0
88	72.5
89	72.0
90	69.5
91	58.5
92	55.0
93	59.0
94	52.5
95	37.5
96	35.5
97	29.5
98	18.0
99	8.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35128287079644654
2	0.0
3	0.0
4	0.019831260998648393
5	0.185027590482535
6	0.04986695726844596
7	0.02483721037694799
8	0.00876041141202429
9	0.007123851038349423
10-11	0.002551108817787293
12-13	0.059638185381857674
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.4440238591248832E-4
92-93	2.8880477182497663E-4
94-95	4.8134128637496106E-5
96-97	2.8880477182497663E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	9.626825727499221E-5
108-109	2.8880477182497663E-4
110-111	1.9253651454998442E-4
112-113	5.294754150124571E-4
114-115	1.9253651454998442E-4
116-117	4.81341286374961E-4
118-119	1.4440238591248832E-4
120-121	1.4440238591248832E-4
122-123	0.001925365145499844
124-125	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1038764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.21947420459789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.1597384341667	58.947123178141055
2	8.244433398637208	11.41350689936987
3	2.6715285884779956	5.547654126509953
4	1.2329015564864607	3.4136318994409263
5	0.7046543956778989	2.438790338239142
6	0.4366218978141536	1.8133642915745631
7	0.3063617897101167	1.4844341400079706
8	0.2234775015901001	1.2375196125299135
9	0.16106880088236022	1.0034187937057844
>10	0.8224873491642699	9.605231810950992
>50	0.026438135262450344	1.2569449292083699
>100	0.009453978451597778	1.0519466963328568
>500	2.780578929017203E-4	0.1453150096454708
>1k	5.561157858034406E-4	0.6411182743431302
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2114	0.20351109587933353	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1719	0.1654851342557116	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1622	0.15614711330003736	No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1207	0.11619578653091558	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	9.62682572749922E-5	0.0	0.0	0.0	0.0
4	9.62682572749922E-5	0.0	0.0	0.0	0.0
5	9.62682572749922E-5	0.0	0.0	0.0	0.0
6	9.62682572749922E-5	0.0	0.0	0.0	0.0
7	9.62682572749922E-5	0.0	0.0	0.0	0.0
8	9.62682572749922E-5	0.0	0.0	0.0	9.62682572749922E-5
9	9.62682572749922E-5	0.0	0.0	0.0	9.62682572749922E-5
10-11	9.62682572749922E-5	0.0	0.0	0.0	9.62682572749922E-5
12-13	9.62682572749922E-5	0.0	0.0	0.0	9.62682572749922E-5
14-15	9.62682572749922E-5	0.0	0.0	0.0	9.62682572749922E-5
16-17	9.62682572749922E-5	0.0	0.0	0.0	9.62682572749922E-5
18-19	9.62682572749922E-5	0.0	0.0	0.0	9.62682572749922E-5
20-21	9.62682572749922E-5	0.0	0.0	0.0	9.62682572749922E-5
22-23	9.62682572749922E-5	0.0	0.0	1.444023859124883E-4	9.62682572749922E-5
24-25	9.62682572749922E-5	0.0	0.0	6.257436722874492E-4	9.62682572749922E-5
26-27	9.62682572749922E-5	0.0	0.0	9.62682572749922E-4	9.62682572749922E-5
28-29	9.62682572749922E-5	0.0	0.0	0.002936181846887262	9.62682572749922E-5
30-31	9.62682572749922E-5	0.0	0.0	0.012659275831661473	9.62682572749922E-5
32-33	9.62682572749922E-5	0.0	0.0	0.035763657577659605	9.62682572749922E-5
34-35	9.62682572749922E-5	0.0	0.0	0.06531801256108222	9.62682572749922E-5
36-37	9.62682572749922E-5	0.0	0.0	0.10310330354151664	9.62682572749922E-5
38-39	9.62682572749922E-5	0.0	0.0	0.17188697336449857	9.62682572749922E-5
40-41	9.62682572749922E-5	0.0	0.0	0.2591060144556415	9.62682572749922E-5
42-43	9.62682572749922E-5	0.0	0.0	0.33549487660334787	9.62682572749922E-5
44-45	9.62682572749922E-5	0.0	0.0	0.4097177029623668	1.444023859124883E-4
46-47	9.62682572749922E-5	0.0	0.0	0.4871173818114606	1.925365145499844E-4
48-49	9.62682572749922E-5	0.0	0.0	0.5762136539194658	1.925365145499844E-4
50-51	1.444023859124883E-4	0.0	0.0	0.673348325509933	1.925365145499844E-4
52-53	1.925365145499844E-4	0.0	0.0	0.7807355665001867	1.925365145499844E-4
54-55	1.925365145499844E-4	0.0	0.0	0.8961130728442649	1.925365145499844E-4
56-57	1.925365145499844E-4	0.0	0.0	1.0114424450597057	1.925365145499844E-4
58-59	1.925365145499844E-4	0.0	0.0	1.1413083241236701	1.925365145499844E-4
60-61	1.925365145499844E-4	0.0	0.0	1.2785387248691715	1.925365145499844E-4
62-63	1.925365145499844E-4	0.0	0.0	1.4304981689777465	1.925365145499844E-4
64-65	1.925365145499844E-4	0.0	0.0	1.568113642752348	2.8880477182497663E-4
66-67	1.925365145499844E-4	0.0	0.0	1.7237794147660104	2.8880477182497663E-4
68-69	1.925365145499844E-4	0.0	0.0	1.9037529217416083	2.8880477182497663E-4
70-71	3.850730290999688E-4	0.0	0.0	2.091812962328306	2.8880477182497663E-4
72-73	3.850730290999688E-4	0.0	0.0	2.2666361175396914	3.369389004624727E-4
74-75	3.850730290999688E-4	0.0	0.0	2.447187234058939	3.850730290999688E-4
76-77	3.850730290999688E-4	0.0	0.0	2.663694544670397	3.850730290999688E-4
78-79	3.850730290999688E-4	0.0	0.0	2.89988871389459	3.850730290999688E-4
80-81	3.850730290999688E-4	0.0	0.0	3.1163478903774102	3.850730290999688E-4
82-83	3.850730290999688E-4	0.0	0.0	3.348065585638316	3.850730290999688E-4
84-85	3.850730290999688E-4	0.0	0.0	3.5974003719805463	3.850730290999688E-4
86-87	3.850730290999688E-4	0.0	0.0	3.868491784466924	3.850730290999688E-4
88-89	3.850730290999688E-4	0.0	0.0	4.179630791979699	3.850730290999688E-4
90-91	3.850730290999688E-4	0.0	0.0	4.5019850514650095	3.850730290999688E-4
92-93	3.850730290999688E-4	0.0	0.0	4.788720055758574	3.850730290999688E-4
94-95	3.850730290999688E-4	0.0	0.0	5.0838785325637	3.850730290999688E-4
96-97	3.850730290999688E-4	0.0	0.0	5.428085686450435	4.81341286374961E-4
98-99	3.850730290999688E-4	0.0	0.0	5.838477267213727	5.776095436499533E-4
100-101	3.850730290999688E-4	0.0	0.0	6.257196052231306	5.776095436499533E-4
102-103	3.850730290999688E-4	0.0	0.0	6.698586011837145	6.738778009249454E-4
104-105	3.850730290999688E-4	0.0	0.0	7.150180406714133	6.738778009249454E-4
106-107	3.850730290999688E-4	0.0	0.0	7.626130670681695	6.738778009249454E-4
108-109	3.850730290999688E-4	0.0	0.0	8.119264818572843	6.738778009249454E-4
110-111	3.850730290999688E-4	0.0	0.0	8.668860299355774	6.738778009249454E-4
112-113	3.850730290999688E-4	0.0	0.0	9.196362215094092	6.738778009249454E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1770	0.0	63.818645	1
GTATCAA	3055	0.0	50.86525	1
GTACATG	6245	0.0	39.525436	1
TACATGG	6440	0.0	38.310276	2
ACATGGG	6865	0.0	35.938553	3
CATGGGG	5745	0.0	35.891106	4
TCAACGC	4290	0.0	35.14504	4
ATGGGGG	3955	0.0	34.806976	5
TATCAAC	4480	0.0	34.585663	2
ATCAACG	4450	0.0	34.14923	3
CAACGCA	4435	0.0	33.727245	5
AACGCAG	4610	0.0	32.691284	6
TGGGGGG	3290	0.0	30.779774	6
GTATACG	80	0.005246011	29.883335	1
ACGCAGA	5530	0.0	26.707563	7
TAACGCA	90	0.009455687	26.486116	4
CGCAGAG	5805	0.0	25.543705	8
TATAGGG	140	1.0445865E-4	25.540182	2
AGAGTAC	5860	0.0	24.33855	10-11
GTGGTAT	890	0.0	24.175282	1
>>END_MODULE