FastQCFastQC Report
Mon 15 Aug 2016
SRR2921308_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921308_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182095
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.051.150661
GTATCAA20850.046.8138771
GTACATG65300.041.2878071
TACATGG69050.038.6958432
ACATGGG71400.037.3388983
TCAACGC26050.037.007434
CAACGCA26600.036.242245
CATGGGG62000.035.80134
TAGTACG1209.277919E-734.7135052
AACGCAG28300.034.273976
ATCAACG29400.032.993013
TATCAAC29600.032.7700842
ATGGGGG37450.032.5749475
GTGTACG2803.6379788E-1227.6326241
GTATATG3450.027.601821
ATGGGGA21450.026.6333395
ACGCAGA36250.026.5864327
GCGTAAA900.00951625926.4517421
TGTACGC1859.247269E-725.7335642
AGTACAT45300.025.6163982
Produced by FastQC (version 0.11.5)