##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921308_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1182095
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.95623701986727	31.0	31.0	34.0	26.0	34.0
2	30.0484411151388	31.0	31.0	34.0	26.0	34.0
3	30.053845080133154	31.0	31.0	34.0	25.0	34.0
4	33.49147741932755	37.0	35.0	37.0	28.0	37.0
5	33.58053878918361	37.0	35.0	37.0	28.0	37.0
6	33.50414391398323	37.0	35.0	37.0	28.0	37.0
7	33.54787305588806	37.0	35.0	37.0	28.0	37.0
8	33.533196570495605	37.0	35.0	37.0	28.0	37.0
9	35.048918234152076	39.0	37.0	39.0	27.0	39.0
10-11	35.0829070421582	39.0	37.0	39.0	27.0	39.0
12-13	35.004879049484174	39.0	37.0	39.0	27.0	39.0
14-15	36.24469226246622	40.0	38.0	41.0	27.0	41.0
16-17	36.21627153485972	40.0	37.0	41.0	27.0	41.0
18-19	36.187909178196335	40.0	37.0	41.0	27.0	41.0
20-21	36.214399434901594	40.0	37.5	41.0	27.0	41.0
22-23	36.15213286580182	40.0	37.0	41.0	27.0	41.0
24-25	36.12786535769122	40.0	37.0	41.0	27.0	41.0
26-27	36.091726130302554	40.0	37.0	41.0	27.0	41.0
28-29	36.02068657764393	40.0	37.0	41.0	27.0	41.0
30-31	35.93700802388979	40.0	37.0	41.0	26.5	41.0
32-33	35.84974642477974	40.0	36.5	41.0	26.0	41.0
34-35	35.71843422059987	40.0	36.0	41.0	25.0	41.0
36-37	35.59671430807168	40.0	36.0	41.0	25.0	41.0
38-39	35.48211522762553	40.0	36.0	41.0	24.0	41.0
40-41	35.36276314509409	40.0	35.0	41.0	24.0	41.0
42-43	35.144211759630146	40.0	35.0	41.0	23.5	41.0
44-45	34.85003447269467	40.0	35.0	41.0	22.5	41.0
46-47	34.598994581653756	39.0	35.0	41.0	20.5	41.0
48-49	34.36651665052301	39.0	34.5	41.0	19.5	41.0
50-51	34.1728981173256	39.0	34.0	40.5	18.0	41.0
52-53	33.96022527800219	38.5	34.0	40.0	17.0	41.0
54-55	33.73347700480926	38.0	34.0	40.0	14.5	41.0
56-57	33.46499266133432	38.0	33.5	40.0	12.5	41.0
58-59	33.17377114360521	37.0	33.0	40.0	9.0	41.0
60-61	32.874856081786994	37.0	33.0	40.0	8.0	41.0
62-63	32.53763318515009	36.0	33.0	39.5	7.0	41.0
64-65	32.18007520546149	36.0	32.0	39.0	4.0	41.0
66-67	31.83788527994789	35.0	32.0	39.0	2.0	40.0
68-69	31.488552104526285	35.0	31.5	38.0	2.0	40.0
70-71	31.448975759139493	35.0	32.0	37.0	2.0	40.0
72-73	31.176303089007227	35.0	32.0	37.0	2.0	39.0
74-75	30.86650057736476	35.0	32.0	36.5	2.0	39.0
76-77	30.589232675884766	35.0	31.5	36.0	2.0	38.5
78-79	30.31670931693307	35.0	31.0	35.5	2.0	37.0
80-81	30.064480858137458	35.0	31.0	35.0	2.0	37.0
82-83	29.856715407814093	35.0	31.0	35.0	2.0	36.0
84-85	29.661453605674673	35.0	31.0	35.0	2.0	36.0
86-87	29.504699283898503	35.0	31.0	35.0	2.0	36.0
88-89	29.364813318726497	34.5	31.0	35.0	2.0	35.0
90-91	29.240640980631845	34.0	30.5	35.0	2.0	35.0
92-93	29.126381128420306	34.0	30.0	35.0	2.0	35.0
94-95	29.005667480194063	34.0	30.0	35.0	2.0	35.0
96-97	28.915174753298167	34.0	30.0	35.0	2.0	35.0
98-99	28.826261848666988	34.0	30.0	35.0	2.0	35.0
100-101	28.725384592608883	34.0	30.0	35.0	2.0	35.0
102-103	27.85487630012816	33.5	28.0	34.5	2.0	35.0
104-105	28.467084286795902	34.0	29.0	35.0	2.0	35.0
106-107	28.55701403017524	34.0	29.0	35.0	2.0	35.0
108-109	28.490658957190412	34.0	29.0	35.0	2.0	35.0
110-111	28.401725749622493	34.0	29.0	35.0	2.0	35.0
112-113	28.298098291592467	34.0	29.0	35.0	2.0	35.0
114-115	28.1606296448255	34.0	29.0	35.0	2.0	35.0
116-117	28.021575254103944	34.0	29.0	35.0	2.0	35.0
118-119	28.016329905802834	34.0	29.0	35.0	2.0	35.0
120-121	28.202292539939684	34.0	30.0	35.0	2.0	35.0
122-123	28.048229626214475	34.0	30.0	35.0	2.0	35.0
124-125	27.42417022320541	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55381.0
3	7431.0
4	4665.0
5	4017.0
6	4259.0
7	4658.0
8	4901.0
9	5129.0
10	5585.0
11	5812.0
12	5802.0
13	5706.0
14	5880.0
15	5190.0
16	4355.0
17	4014.0
18	3934.0
19	3737.0
20	3730.0
21	3798.0
22	4341.0
23	5533.0
24	7574.0
25	9144.0
26	10132.0
27	11651.0
28	13923.0
29	16674.0
30	20820.0
31	25968.0
32	33819.0
33	46552.0
34	72772.0
35	118914.0
36	237364.0
37	329978.0
38	68931.0
39	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.56248514031351	17.235205674983014	11.413846816410002	25.78846236829347
2	17.285589433938	19.25906936960489	37.38156455350503	26.07377664295208
3	20.3277049149815	23.474887011433378	27.72818106597585	28.469227007609277
4	14.252717966073014	14.368458902660858	33.37027793053851	38.00854520072761
5	15.013303666228145	37.54823372151556	32.207224165718415	15.231238446537873
6	35.128326421051476	33.33821325482583	16.36446412961523	15.168996194507464
7	30.90958008902306	31.567142522342483	18.594329158562058	18.928948230072397
8	29.149410505564983	33.5230488728325	18.498999530435004	18.828541091167526
9	28.615409866023235	15.39827545947702	17.50564299709982	38.480671677399926
10-11	27.278384094754653	25.112351945854485	25.562140439932318	22.047123519458545
12-13	26.854371084027484	22.476518970100287	26.07083817674519	24.598271769127038
14-15	24.551595019724658	23.92021982651776	23.070490168127016	28.457694985630567
16-17	25.063290817977514	26.833064942195445	23.797601357310455	24.30604288251659
18-19	24.98608321644363	26.202173217848003	25.101689810545626	23.710053755162747
20-21	25.795654173052217	24.954009223958266	25.044869823579003	24.205466779410514
22-23	25.856448894784574	24.371421416679077	24.972293474288342	24.799836214248007
24-25	25.32465335059178	24.61938360278504	24.97512753483414	25.08083551178904
26-27	24.791864945919993	25.092065022905217	25.175902778659022	24.940167252515767
28-29	24.914511186725168	25.488652533869814	25.079016874417313	24.517819404987705
30-31	25.546771232540628	24.948393864791925	25.007444819505427	24.497390083162017
32-33	25.169701417721196	24.91957504319999	24.93230767278411	24.978415866294696
34-35	25.168633243739652	25.238853031675895	25.103827993668375	24.488685730916078
36-37	25.03609307338706	25.369761005267705	25.097640593783403	24.496505327561827
38-39	25.132919378670376	25.428220574468902	25.068157004974985	24.37070304188573
40-41	25.1128375503063	25.10894587097053	25.130307806454944	24.64790877226823
42-43	24.87327767407056	25.077420858560412	24.948995929403512	25.10030553796551
44-45	24.67608224275029	25.14109404888102	25.156660713545453	25.02616299482324
46-47	24.68364711287011	25.11981942271785	25.12756062460924	25.068972839802804
48-49	24.566826212296505	25.426257558676905	25.147197235200437	24.859718993826156
50-51	24.61904520189843	25.348341412654502	25.307817906482917	24.724795478964154
52-53	24.87576478470972	25.126730917606295	25.126815517684804	24.87068877999919
54-55	24.409065452028976	24.934519206853317	25.339628739130948	25.316786601986756
56-57	24.63115763077946	25.234664033860994	24.916223142124974	25.21795519323457
58-59	24.68079850289168	25.444031025065733	25.115396459596013	24.759774012446574
60-61	24.99473366738943	25.291128788692742	25.243626045546797	24.470511498371035
62-63	24.85056422026692	25.217433236649338	25.17073389454324	24.761268648540497
64-65	24.625060064836184	24.959392786805363	25.218348369281994	25.19719877907646
66-67	24.740112956113606	25.232900075461995	24.949748403623527	25.077238564800876
68-69	24.442754304287835	25.277512459613593	25.162581988952798	25.117151247145774
70-71	24.390164461439547	25.0614615410646	25.445416398768234	25.10295759872762
72-73	24.47620570399848	25.491223706651507	25.037477327485856	24.995093261864156
74-75	24.298264500319995	25.421777308349085	25.212733962944935	25.06722422838599
76-77	24.45180647210779	24.972473470686097	25.090362732611332	25.48535732459478
78-79	24.21006681544623	25.111034784977438	25.386401051721485	25.292497347854848
80-81	24.484005273837276	25.386792480763482	25.08803475961276	25.041167485786488
82-83	24.486827149991942	25.20406570541359	25.421022331431388	24.88808481316308
84-85	24.48806586868902	25.299749236032852	25.046574076894178	25.165610818383943
86-87	24.18715006759649	25.534511435566753	25.135108382811033	25.143230114025723
88-89	24.223283003783706	25.18052438892849	25.302897315451855	25.293295291835953
90-91	24.459025713304356	25.001184399095116	25.398930995216716	25.140858892383804
92-93	24.50681132519234	25.02406933321602	25.425506894235372	25.043612447356267
94-95	24.54148213009141	24.944395798699677	25.306235538466094	25.20788653274282
96-97	24.190825399336628	24.917987988915318	25.39688314171464	25.494303470033415
98-99	24.015475242741854	25.074893169895184	25.282969703481424	25.626661883881535
100-101	24.082233502538074	25.251565143824028	25.353807106598985	25.312394247038917
102-103	23.773726011728915	25.181551154110366	25.55319804134561	25.491524792815106
104-105	23.833838110385436	25.236624818111064	25.462843896991643	25.46669317451186
106-107	24.044224612027886	25.016507876615414	25.52327113568692	25.41599637566978
108-109	23.93573865688903	25.11376739928444	25.456528967011472	25.493964976815054
110-111	23.725471782715147	25.59902403202691	25.447036647868117	25.228467537389825
112-113	23.64850602922334	25.66016474138445	25.49037274292755	25.200956486464648
114-115	23.61001310835521	25.67097309339841	25.41464320444508	25.304370593801302
116-117	23.54613228995929	25.57601649658965	25.500851266324748	25.376999947126315
118-119	23.582811689068244	25.390401745522333	25.586052768156925	25.440733797252495
120-121	23.476682798064232	25.484068834816746	25.70674980540797	25.33249856171106
122-123	23.342392281353302	25.631168984981883	25.589293380905147	25.437145352759664
124-125	23.267099745448487	25.5104354277506	25.513184820980662	25.709280005820258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	333.0
1	275.0
2	206.5
3	162.0
4	126.5
5	118.0
6	118.0
7	155.0
8	215.5
9	258.5
10	285.0
11	285.0
12	296.5
13	350.0
14	379.5
15	394.5
16	421.0
17	467.0
18	522.0
19	545.5
20	592.0
21	663.5
22	721.5
23	834.0
24	964.0
25	1189.5
26	1539.5
27	1990.0
28	2544.0
29	3276.5
30	4356.0
31	5534.5
32	6964.0
33	8558.0
34	10137.0
35	11826.0
36	14008.5
37	16451.0
38	18488.5
39	20643.0
40	22975.5
41	25178.5
42	27445.0
43	30349.5
44	33816.0
45	37222.5
46	40613.5
47	44011.5
48	47826.0
49	51145.0
50	52844.5
51	54787.5
52	56131.0
53	56278.5
54	56675.0
55	56045.5
56	53520.5
57	49351.5
58	44840.0
59	40016.5
60	34531.0
61	29178.5
62	24275.0
63	18973.5
64	13893.0
65	10437.0
66	7874.5
67	5978.0
68	4654.0
69	3554.0
70	2736.0
71	2120.0
72	1540.5
73	1195.0
74	952.5
75	677.0
76	467.0
77	289.5
78	173.5
79	121.5
80	85.5
81	66.0
82	49.5
83	31.0
84	26.0
85	25.5
86	17.5
87	10.0
88	12.5
89	10.5
90	6.5
91	6.5
92	4.0
93	2.5
94	4.5
95	5.0
96	2.5
97	1.0
98	1.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01742668736438273
2	0.010320659507061616
3	0.00989768165841155
4	0.01226635761085192
5	0.007613601275701191
6	0.03231550763686506
7	0.013027717738422038
8	0.012689335459501986
9	0.00837496140327131
10-11	0.008036579124351257
12-13	0.006513858869211019
14-15	0.006090881020560953
16-17	0.004356671841095682
18-19	0.0058370943113709136
20-21	0.005456414247585854
22-23	0.005329520892990834
24-25	0.005498712032450861
26-27	0.004229778486500662
28-29	0.0058370943113709136
30-31	0.005498712032450861
32-33	0.007825090200026225
34-35	0.007782792415161218
36-37	0.006894538932996079
38-39	0.006894538932996079
40-41	0.007275218996781139
42-43	0.006894538932996079
44-45	0.006936836717861086
46-47	0.00913632153084143
48-49	0.007825090200026225
50-51	0.005498712032450861
52-53	0.005329520892990834
54-55	0.0058370943113709136
56-57	0.007275218996781139
58-59	0.007021432287591099
60-61	0.004906543044340768
62-63	0.005541009817315867
64-65	0.003299227219470516
66-67	0.0036376094983905697
68-69	0.006429263299481006
70-71	0.0046527563351507276
72-73	0.003975991777310622
74-75	0.003679907283255576
76-77	0.004525862980555708
78-79	0.002791653801090437
80-81	0.0028339515859554434
82-83	0.0052026275383958145
84-85	0.009051725961111416
86-87	0.006852241148131073
88-89	0.004441267410825695
90-91	0.005160329753530808
92-93	0.007867387984891231
94-95	0.00676764557840106
96-97	0.00875564146705637
98-99	0.006598454438941032
100-101	0.008036579124351257
102-103	0.004991138614070781
104-105	0.0046527563351507276
106-107	0.007063730072456105
108-109	0.006598454438941032
110-111	0.007529005705971178
112-113	0.005118031968665801
114-115	0.0020725914583853244
116-117	0.0027493560162254304
118-119	0.16348093850325057
120-121	0.011420401913551786
122-123	0.0019034003189252978
124-125	0.001353529115680212
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1182095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.58048159483879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.83513363213743	55.152030902005365
2	9.463235049048706	12.601334940216413
3	3.0304216778546023	6.053008042409965
4	1.3997532823817633	3.727849906197367
5	0.8456186159119746	2.8150847346490138
6	0.5541292960835801	2.2136517239432267
7	0.35121744124978316	1.6368958468042292
8	0.2708585929052594	1.4427116447786044
9	0.20965129315735914	1.256281565885793
>10	1.008128950070767	11.25101981234186
>50	0.027019531635114075	1.2142358548130723
>100	0.004578290797865637	0.5241271269047857
>500	2.5434676580148084E-4	0.11176789905040495
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5378670919003974E-4
2	0.0	0.0	0.0	0.0	2.5378670919003974E-4
3	0.0	0.0	0.0	0.0	2.5378670919003974E-4
4	0.0	0.0	0.0	0.0	2.5378670919003974E-4
5	0.0	0.0	0.0	0.0	2.5378670919003974E-4
6	0.0	0.0	0.0	0.0	2.5378670919003974E-4
7	0.0	0.0	0.0	0.0	2.5378670919003974E-4
8	0.0	0.0	0.0	0.0	2.5378670919003974E-4
9	8.459556973001323E-5	0.0	0.0	0.0	3.3838227892005293E-4
10-11	8.459556973001323E-5	0.0	0.0	0.0	3.3838227892005293E-4
12-13	8.459556973001323E-5	0.0	0.0	8.459556973001323E-5	3.3838227892005293E-4
14-15	8.459556973001323E-5	0.0	0.0	8.459556973001323E-5	4.229778486500662E-4
16-17	8.459556973001323E-5	0.0	0.0	8.459556973001323E-5	5.075734183800795E-4
18-19	8.459556973001323E-5	0.0	0.0	8.459556973001323E-5	5.075734183800795E-4
20-21	8.459556973001323E-5	0.0	0.0	1.2689335459501984E-4	5.075734183800795E-4
22-23	8.459556973001323E-5	0.0	0.0	2.114889243250331E-4	5.498712032450861E-4
24-25	8.459556973001323E-5	0.0	0.0	5.921689881100927E-4	5.921689881100927E-4
26-27	8.459556973001323E-5	0.0	0.0	0.0016496136097352582	5.921689881100927E-4
28-29	8.459556973001323E-5	0.0	0.0	0.005456414247585854	5.921689881100927E-4
30-31	8.459556973001323E-5	0.0	0.0	0.02114889243250331	5.921689881100927E-4
32-33	8.459556973001323E-5	0.0	0.0	0.05139180861098304	6.767645578401059E-4
34-35	8.459556973001323E-5	0.0	0.0	0.09034806847165414	8.459556973001324E-4
36-37	8.459556973001323E-5	0.0	0.0	0.14161298372804215	8.459556973001324E-4
38-39	8.459556973001323E-5	0.0	0.0	0.22210566832614975	8.459556973001324E-4
40-41	8.459556973001323E-5	0.0	0.0	0.31461092382591926	9.305512670301457E-4
42-43	8.459556973001323E-5	0.0	0.0	0.4030978897635131	9.728490518951523E-4
44-45	8.459556973001323E-5	0.0	0.0	0.510830347814685	0.0010574446216251657
46-47	8.459556973001323E-5	0.0	0.0	0.6270646606237231	0.0010997424064901722
48-49	8.459556973001323E-5	0.0	0.0	0.7380963458943655	0.0012689335459501985
50-51	8.459556973001323E-5	0.0	0.0	0.8489588400255479	0.0012689335459501985
52-53	8.459556973001323E-5	0.0	0.0	0.964389494922151	0.0014804224702752316
54-55	8.459556973001323E-5	0.0	0.0	1.104479758395053	0.0016496136097352582
56-57	8.459556973001323E-5	0.0	0.0	1.252860387701496	0.0017765069643302781
58-59	8.459556973001323E-5	0.0	0.0	1.4058091777733601	0.0018611025340602913
60-61	8.459556973001323E-5	0.0	0.0	1.552667086824663	0.0018611025340602913
62-63	8.459556973001323E-5	0.0	0.0	1.7073500860759923	0.0019456981037903045
64-65	8.459556973001323E-5	0.0	0.0	1.8729882116073582	0.0019456981037903045
66-67	8.459556973001323E-5	0.0	0.0	2.073860391931275	0.0019456981037903045
68-69	8.459556973001323E-5	0.0	0.0	2.2839534893557625	0.0019456981037903045
70-71	8.459556973001323E-5	0.0	0.0	2.48998599943321	0.0019456981037903045
72-73	8.459556973001323E-5	0.0	0.0	2.6865438057008957	0.0025801648767654036
74-75	8.459556973001323E-5	0.0	0.0	2.895029587300513	0.0026224626616304105
76-77	8.459556973001323E-5	0.0	0.0	3.1522424170646186	0.0026224626616304105
78-79	8.459556973001323E-5	0.0	0.0	3.438344633891523	0.0026224626616304105
80-81	8.459556973001323E-5	0.0	0.0	3.706850972214585	0.0026224626616304105
82-83	8.459556973001323E-5	0.0	0.0	3.975230417183052	0.0027493560162254304
84-85	8.459556973001323E-5	0.0	0.0	4.262939949834827	0.002791653801090437
86-87	8.459556973001323E-5	0.0	0.0	4.594554583176478	0.002791653801090437
88-89	8.459556973001323E-5	0.0	0.0	4.976757367216679	0.002791653801090437
90-91	8.459556973001323E-5	0.0	0.0	5.354645777200648	0.002791653801090437
92-93	8.459556973001323E-5	0.0	0.0	5.715699668808345	0.0028762493708204503
94-95	8.459556973001323E-5	0.0	0.0	6.080856445547947	0.0029185471556854568
96-97	8.459556973001323E-5	0.0	0.0	6.499519920141783	0.00300314272541547
98-99	8.459556973001323E-5	0.0	0.0	6.981630072033128	0.0031300360800104897
100-101	8.459556973001323E-5	0.0	0.0	7.468731362538543	0.0031300360800104897
102-103	8.459556973001323E-5	0.0	0.0	7.946611735943389	0.003172333864875496
104-105	8.459556973001323E-5	0.0	0.0	8.448348060012098	0.00325692943460551
106-107	8.459556973001323E-5	0.0	0.0	8.992424466730677	0.003341525004335523
108-109	8.459556973001323E-5	0.0	0.0	9.603162182396508	0.0035107161437955493
110-111	8.459556973001323E-5	0.0	0.0	10.242070222782433	0.0037222050681205827
112-113	8.459556973001323E-5	0.0	0.0	10.849424115659062	0.0037222050681205827
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1245	0.0	51.15066	1
GTATCAA	2085	0.0	46.813877	1
GTACATG	6530	0.0	41.287807	1
TACATGG	6905	0.0	38.695843	2
ACATGGG	7140	0.0	37.338898	3
TCAACGC	2605	0.0	37.00743	4
CAACGCA	2660	0.0	36.24224	5
CATGGGG	6200	0.0	35.8013	4
TAGTACG	120	9.277919E-7	34.713505	2
AACGCAG	2830	0.0	34.27397	6
ATCAACG	2940	0.0	32.99301	3
TATCAAC	2960	0.0	32.770084	2
ATGGGGG	3745	0.0	32.574947	5
GTGTACG	280	3.6379788E-12	27.632624	1
GTATATG	345	0.0	27.60182	1
ATGGGGA	2145	0.0	26.633339	5
ACGCAGA	3625	0.0	26.586432	7
GCGTAAA	90	0.009516259	26.451742	1
TGTACGC	185	9.247269E-7	25.733564	2
AGTACAT	4530	0.0	25.616398	2
>>END_MODULE