FastQCFastQC Report
Mon 15 Aug 2016
SRR2921308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182095
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16220.13721401410208148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.10362957291926622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.069.687441
GTATCAA28800.049.797481
GTGGTAT7300.040.1108471
GTACATG63450.037.1067771
TACATGG66900.034.9240342
ACATGGG68400.033.983883
TCAACGC40050.033.7821924
TATCAAC42550.033.4782522
TGGTATC8300.033.032712
CATGGGG59350.032.7386934
CAACGCA40950.032.6030775
ATCAACG44050.032.067633
AACGCAG42300.031.4189856
ATGGGGG37600.029.4842135
GTAAGTA4750.026.3505782
GTATAGG2506.675691E-1026.293071
ACGCAGA52500.024.5138597
CGCAGAG54600.023.455938
GTACACT9700.023.349434
TACACTG13300.022.4069525
Produced by FastQC (version 0.11.5)