##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921308_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1182095
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.252503394397234	33.0	30.0	34.0	19.0	34.0
2	30.00680909740757	33.0	31.0	34.0	26.0	34.0
3	30.248760040436682	33.0	31.0	34.0	26.0	34.0
4	32.42207098414256	37.0	35.0	37.0	19.0	37.0
5	32.109153663622635	37.0	33.0	37.0	19.0	37.0
6	32.26208638053625	37.0	35.0	37.0	17.0	37.0
7	32.473140483632875	37.0	35.0	37.0	17.0	37.0
8	32.71827729581802	37.0	35.0	37.0	17.0	37.0
9	34.44826600230946	39.0	37.0	39.0	17.0	39.0
10-11	34.636646377829194	39.0	37.0	39.0	24.0	39.0
12-13	34.67652515237777	39.0	37.0	39.0	25.0	39.0
14-15	36.06758382363516	41.0	38.0	41.0	26.0	41.0
16-17	36.091859368324876	41.0	38.0	41.0	26.0	41.0
18-19	36.112989226754195	41.0	38.0	41.0	26.0	41.0
20-21	36.10535447658606	41.0	38.0	41.0	26.0	41.0
22-23	36.06353507966788	41.0	38.0	41.0	26.0	41.0
24-25	36.008959939767955	41.0	38.0	41.0	25.0	41.0
26-27	35.91819354620398	40.5	38.0	41.0	25.0	41.0
28-29	35.83516341749183	40.0	37.5	41.0	24.5	41.0
30-31	35.728037509675616	40.0	37.0	41.0	24.0	41.0
32-33	35.58896070112809	40.0	37.0	41.0	23.0	41.0
34-35	35.495289718677434	40.0	36.5	41.0	22.5	41.0
36-37	35.40849339520089	40.0	36.0	41.0	21.5	41.0
38-39	35.30136748738468	40.0	36.0	41.0	19.5	41.0
40-41	35.161674400111664	40.0	35.5	41.0	17.5	41.0
42-43	35.00668897169855	40.0	35.0	41.0	15.5	41.0
44-45	34.841400225870174	40.0	35.0	41.0	11.5	41.0
46-47	34.60269140805096	40.0	35.0	41.0	7.5	41.0
48-49	34.37365524767468	40.0	35.0	41.0	2.0	41.0
50-51	34.17554342079106	39.5	35.0	41.0	2.0	41.0
52-53	33.978699258519825	39.0	35.0	41.0	2.0	41.0
54-55	33.765781515022056	39.0	35.0	41.0	2.0	41.0
56-57	33.512871215934425	38.5	34.0	41.0	2.0	41.0
58-59	33.427568850219316	38.0	34.5	41.0	2.0	41.0
60-61	33.1718791636882	37.5	34.0	40.5	2.0	41.0
62-63	32.87147479686489	37.0	34.0	40.0	2.0	41.0
64-65	32.56173657785541	36.0	34.0	39.5	2.0	41.0
66-67	32.23621155660078	36.0	34.0	39.0	2.0	41.0
68-69	31.90226546935737	35.0	33.5	39.0	2.0	41.0
70-71	31.580501567132927	35.0	33.0	37.5	2.0	40.0
72-73	31.248815027557008	35.0	33.0	37.0	2.0	39.0
74-75	30.92776342002969	35.0	33.0	36.5	2.0	39.0
76-77	30.647810455166464	35.0	33.0	36.0	2.0	38.5
78-79	30.40991925352869	35.0	33.0	36.0	2.0	37.0
80-81	30.199970391550593	35.0	32.5	35.0	2.0	37.0
82-83	30.012792542054573	35.0	32.0	35.0	2.0	36.5
84-85	29.85612070095889	35.0	32.0	35.0	2.0	36.0
86-87	29.729943447861636	35.0	32.0	35.0	2.0	36.0
88-89	29.614900240674395	35.0	32.0	35.0	2.0	35.5
90-91	29.525088085136982	35.0	32.0	35.0	2.0	35.0
92-93	29.45987124554287	35.0	32.0	35.0	2.0	35.0
94-95	29.392906661478136	35.0	32.0	35.0	2.0	35.0
96-97	29.331393838904656	35.0	32.0	35.0	2.0	35.0
98-99	29.281144916440727	35.0	31.5	35.0	2.0	35.0
100-101	29.222817540045426	35.0	31.0	35.0	2.0	35.0
102-103	28.54288572407463	34.0	29.5	35.0	2.0	35.0
104-105	29.087310664540496	35.0	31.0	35.0	2.0	35.0
106-107	29.146591855984504	35.0	31.0	35.0	2.0	35.0
108-109	29.213390632732562	35.0	31.5	35.0	2.0	35.0
110-111	29.324165570449075	35.0	32.0	35.0	2.0	35.0
112-113	29.279955079752472	35.0	32.0	35.0	2.0	35.0
114-115	29.21358181872015	35.0	32.0	35.0	2.0	35.0
116-117	29.146086397455363	35.0	32.0	35.0	2.0	35.0
118-119	29.05557844335692	35.0	32.0	35.0	2.0	35.0
120-121	28.949322600975385	35.0	32.0	35.0	2.0	35.0
122-123	28.831008506084537	35.0	32.0	35.0	2.0	35.0
124-125	28.297072147331644	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77435.0
3	4868.0
4	3752.0
5	3499.0
6	3751.0
7	4042.0
8	4124.0
9	4056.0
10	4199.0
11	4273.0
12	4847.0
13	5995.0
14	7305.0
15	6143.0
16	3957.0
17	3374.0
18	2893.0
19	2844.0
20	2755.0
21	2717.0
22	3074.0
23	3973.0
24	4993.0
25	6533.0
26	7745.0
27	8934.0
28	10734.0
29	12832.0
30	15604.0
31	19387.0
32	25138.0
33	33492.0
34	51239.0
35	88096.0
36	201663.0
37	405295.0
38	126322.0
39	212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.449641952093316	17.294032251902728	11.186291031186462	27.070034764817496
2	17.029764951209504	19.465863572724697	36.15648488488658	27.347886591179222
3	19.611621739369507	22.74571840672704	28.126165832695342	29.51649402120811
4	14.312499629827997	15.28027732017467	30.13301655070764	40.2742064992897
5	14.891141201586883	37.53524594401499	31.606384484411272	15.967228369986856
6	35.35327032244904	33.0841844486068	15.862187460060992	15.700357768883164
7	31.30517634853894	31.27276758175598	18.12843179646956	19.293624273235523
8	29.280844806844	33.883813678195736	17.53023270766956	19.305108807290704
9	28.60542614869329	15.316784783975187	17.254574172791084	38.823214894540435
10-11	27.717690153713402	25.02216450802406	24.744008392058	22.51613694620454
12-13	27.317263923051012	22.506047612356937	25.61483353619003	24.561854928402024
14-15	24.99130780521024	23.645053908526812	23.005722890292233	28.35791539597071
16-17	24.98107174127291	26.613808534847028	23.68963577377453	24.715483950105533
18-19	24.940212081093314	25.592866901560363	25.558563398034845	23.908357619311477
20-21	25.640325016178906	24.418215118074265	25.735495032125165	24.205964833621664
22-23	25.60724814841447	23.92447307534504	25.507086993854134	24.961191782386358
24-25	25.11913171107229	24.339202855946436	25.47946654033728	25.062198892643995
26-27	24.56101243977853	24.75981202864406	25.768825686598795	24.91034984497862
28-29	24.681391935504337	24.962460715932306	25.7278391330646	24.628308215498755
30-31	25.23959580236783	24.61646483573655	25.619345314885862	24.524594047009757
32-33	24.987797089066447	24.6415474221615	25.4958780808649	24.874777407907146
34-35	25.05936494105804	24.82308951480211	25.68913665991312	24.428408884226734
36-37	24.964913987454477	24.885901725326644	25.761085191968498	24.388099095250382
38-39	24.851555924016257	25.072815636645107	25.69116695358664	24.38446148575199
40-41	24.88839729463368	24.824400746132923	25.687021770669872	24.600180188563524
42-43	24.716879777006078	24.774447062207354	25.42223763741493	25.08643552337164
44-45	24.545193068239016	24.89795659401317	25.630173547811303	24.92667678993651
46-47	24.607540003130033	24.927607341203544	25.260702397015468	25.204150258650955
48-49	24.582753501199143	25.242345158384055	25.118920222147967	25.05598111826884
50-51	24.523282815678943	25.293144798006928	25.331974164513007	24.851598221801126
52-53	24.813741704346942	24.98014119000588	25.19852465326391	25.00759245238327
54-55	24.37278729712925	24.793227278687414	25.44254057415013	25.391444850033203
56-57	24.498623207102643	25.139434647807494	25.030517851780104	25.331424293309755
58-59	24.537748658102775	25.250762417572194	25.199328311176345	25.012160613148687
60-61	24.849314141418414	25.044814503064472	25.440721769400938	24.665149586116176
62-63	24.669633151311864	25.065963395496976	25.31818508664701	24.946218366544144
64-65	24.515923001112434	24.79166226064741	25.29678240750532	25.39563233073484
66-67	24.529373696699505	25.085251185395418	25.105131144281973	25.2802439736231
68-69	24.249150871968837	25.117270608538234	25.291156802118277	25.342421717374663
70-71	24.269242319779714	24.980098892221015	25.447362521624743	25.30329626637453
72-73	24.328713005299914	25.302534906246958	25.080556131275404	25.288195957177724
74-75	24.14865133512958	25.368307961712045	25.167478079172994	25.315562623985382
76-77	24.251350356781813	24.863906877196843	25.11807426645067	25.766668499570677
78-79	24.02201176724375	25.025484415381165	25.32237256734865	25.630131250026437
80-81	24.255157157419667	25.336246240784373	24.956962003899854	25.451634597896106
82-83	24.243482968796922	25.17031203075895	25.218404612150465	25.367800388293666
84-85	24.223814498834695	25.173738151333012	24.92726895892462	25.675178390907664
86-87	23.890338762958983	25.487460821676766	25.002812802693526	25.619387612670725
88-89	23.84038507903341	25.20051264915256	25.11591707942255	25.843185192391477
90-91	24.076626829118702	24.93768465665758	25.28008594928062	25.7056025649431
92-93	24.079301780233685	24.998477275880386	25.248246329388913	25.67397461449701
94-95	24.082824243598328	24.95276388881252	25.132741191792356	25.831670675796797
96-97	23.78588130196296	24.821587490652167	25.198419412363844	26.19411179502103
98-99	23.64371038017688	24.932186047731378	25.13449643831352	26.28960713377822
100-101	23.714718360199477	25.18786561147793	25.149501520605366	25.94791450771723
102-103	23.432211455086097	24.980014296651284	25.302450310677234	26.285323937585385
104-105	23.4805578231868	25.084701314192177	25.251523777699763	26.18321708492126
106-107	23.62250685859742	24.88666289369787	25.2442489719506	26.246581275754107
108-109	23.560739587417725	24.923832038382816	25.197161133465556	26.3182672407339
110-111	23.399626847837073	25.39883756883456	25.148814622218328	26.052720961110033
112-113	23.252404536546965	25.403977790156993	25.203696677533028	26.139920995763006
114-115	23.175386020975516	25.416454296089352	25.18153190211426	26.226627780820877
116-117	22.992421470624684	25.307812987087697	25.27469366744227	26.425071874845347
118-119	23.167434020603295	25.091775812806528	25.22543709565876	26.51535307093142
120-121	23.06690500078674	25.22371317340245	25.303317671860277	26.40606415395053
122-123	22.89100925316518	25.2997676980108	25.15929520963859	26.649927839185438
124-125	22.596764724294346	25.334279676573946	25.061797115965394	27.007158483166315
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	35.5
2	39.5
3	49.0
4	49.0
5	58.5
6	146.0
7	320.0
8	448.0
9	545.5
10	547.5
11	451.0
12	451.0
13	520.5
14	566.5
15	615.0
16	685.0
17	731.0
18	795.5
19	863.5
20	937.0
21	1010.0
22	1039.5
23	1103.5
24	1228.5
25	1436.0
26	1737.0
27	2133.5
28	2552.5
29	3260.5
30	4276.0
31	5351.0
32	6678.0
33	8127.0
34	9486.5
35	10985.5
36	13012.0
37	15232.0
38	17258.0
39	19489.5
40	21952.0
41	24224.5
42	26474.0
43	29282.0
44	32660.5
45	35971.5
46	39568.0
47	43296.0
48	47111.0
49	50714.0
50	53231.5
51	55651.0
52	56954.0
53	57001.0
54	57038.5
55	56614.0
56	54216.0
57	50051.0
58	45765.5
59	40936.5
60	35421.0
61	30181.0
62	25087.0
63	19316.0
64	14174.5
65	10630.5
66	7930.0
67	6036.0
68	4713.0
69	3539.0
70	2732.5
71	2129.5
72	1618.0
73	1285.0
74	979.0
75	709.5
76	530.0
77	367.0
78	258.0
79	174.5
80	126.0
81	125.5
82	115.0
83	93.5
84	97.0
85	92.5
86	71.0
87	68.5
88	59.5
89	53.0
90	57.5
91	58.5
92	56.5
93	48.5
94	42.0
95	43.0
96	33.5
97	29.0
98	22.0
99	8.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3536094814714553
2	0.0
3	0.0
4	0.017934260782762806
5	0.18374157745358874
6	0.05126491525638802
7	0.02673220003468418
8	0.008205770263811285
9	0.006598454438941032
10-11	0.002114889243250331
12-13	0.053887377918018425
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.1148892432503312E-4
92-93	2.5378670919003974E-4
94-95	1.2689335459501987E-4
96-97	2.5378670919003974E-4
98-99	4.2297784865006624E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.691911394600265E-4
108-109	2.9608449405504634E-4
110-111	2.9608449405504634E-4
112-113	5.498712032450861E-4
114-115	2.1148892432503312E-4
116-117	4.6527563351507283E-4
118-119	2.1148892432503312E-4
120-121	8.459556973001325E-5
122-123	0.0010997424064901722
124-125	8.459556973001325E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1182095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.73772448596863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.60752558918624	55.797760077246835
2	8.939327703261576	11.931807787001155
3	2.9054501114152345	5.817093871300703
4	1.3528011062377088	3.6113147004962314
5	0.7987483340199102	2.665332312472361
6	0.5379160151397158	2.153957448899069
7	0.3791187822241031	1.7711067384869696
8	0.2573962242293653	1.3742430637078389
9	0.20555651381366594	1.2346536586673378
>10	0.978031499297411	11.170088485091227
>50	0.031530819724306984	1.4162070382503245
>100	0.005836080265932776	0.5479897699250424
>500	3.8061059240001216E-4	0.16942523596306225
>1k	3.8061059240001216E-4	0.3390198124918047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1622	0.13721401410208148	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1225	0.10362957291926622	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	8.459556973001323E-5	2.114889243250331E-4
14-15	0.0	0.0	0.0	8.459556973001323E-5	4.229778486500662E-4
16-17	0.0	0.0	0.0	8.459556973001323E-5	5.921689881100927E-4
18-19	0.0	0.0	0.0	8.459556973001323E-5	5.921689881100927E-4
20-21	0.0	0.0	0.0	1.2689335459501984E-4	6.344667729750993E-4
22-23	0.0	0.0	0.0	2.114889243250331E-4	7.613601275701192E-4
24-25	0.0	0.0	0.0	5.921689881100927E-4	9.305512670301457E-4
26-27	0.0	0.0	0.0	0.0016919113946002647	0.001015146836760159
28-29	0.0	0.0	0.0	0.005498712032450861	0.001015146836760159
30-31	0.0	0.0	0.0	0.01992225667141812	0.001015146836760159
32-33	0.0	0.0	0.0	0.0485578570250276	0.0010997424064901722
34-35	0.0	0.0	0.0	0.08493395200893329	0.0010997424064901722
36-37	0.0	0.0	0.0	0.13256125776693073	0.0010997424064901722
38-39	0.0	0.0	0.0	0.20865497273907765	0.0012689335459501985
40-41	0.0	0.0	0.0	0.29625368519450634	0.0013535291156802117
42-43	0.0	0.0	0.0	0.37979181030289444	0.0013535291156802117
44-45	0.0	0.0	0.0	0.48266002309459055	0.0013535291156802117
46-47	0.0	0.0	0.0	0.5917460102614427	0.0015227202551402383
48-49	0.0	0.0	0.0	0.697067494575309	0.0016073158248702515
50-51	0.0	0.0	0.0	0.8026850633832306	0.0016073158248702515
52-53	0.0	0.0	0.0	0.9119825394744077	0.0017342091794652712
54-55	0.0	0.0	0.0	1.0462357086359386	0.0018188047491952846
56-57	0.0	0.0	0.0	1.187594905654791	0.0018611025340602913
58-59	0.0	0.0	0.0	1.3341144324271736	0.0019456981037903045
60-61	0.0	0.0	0.0	1.4745853759638607	0.0019456981037903045
62-63	0.0	0.0	0.0	1.6239811521070642	0.0019456981037903045
64-65	0.0	0.0	0.0	1.782301760856784	0.0019456981037903045
66-67	0.0	0.0	0.0	1.9731916639525588	0.001987995888655311
68-69	0.0	0.0	0.0	2.17317559079431	0.002030293673520318
70-71	0.0	0.0	0.0	2.3689297391495607	0.002030293673520318
72-73	0.0	0.0	0.0	2.5530942944517996	0.002114889243250331
74-75	0.0	0.0	0.0	2.7488484428070503	0.002114889243250331
76-77	0.0	0.0	0.0	2.9929489592630034	0.002114889243250331
78-79	0.0	0.0	0.0	3.2672500941125717	0.0021994848129803443
80-81	0.0	0.0	0.0	3.525647261852897	0.0022840803827103573
82-83	0.0	0.0	0.0	3.7815065625013218	0.002453271522170384
84-85	0.0	0.0	0.0	4.054919443868725	0.002453271522170384
86-87	0.0	0.0	0.0	4.369995643328159	0.002453271522170384
88-89	0.0	0.0	0.0	4.733883486521811	0.002537867091900397
90-91	0.0	0.0	0.0	5.0959102271814025	0.002537867091900397
92-93	0.0	0.0	0.0	5.4383107956636305	0.0026224626616304105
94-95	0.0	0.0	0.0	5.785871693899391	0.002791653801090437
96-97	0.0	0.0	0.0	6.185331974164513	0.0028339515859554434
98-99	0.0	0.0	0.0	6.648027442802821	0.0028762493708204503
100-101	0.0	0.0	0.0	7.116940685816283	0.0028762493708204503
102-103	0.0	0.0	0.0	7.5768445006535	0.0029608449405504637
104-105	0.0	0.0	0.0	8.060858052863772	0.0030454405102804767
106-107	0.0	0.0	0.0	8.588903599118513	0.003087738295145483
108-109	0.0	0.0	0.0	9.179592164758333	0.003214631649740503
110-111	0.0	0.0	0.0	9.794263574416608	0.0032992272194705165
112-113	0.0	0.0	0.0	10.38567120239913	0.0032992272194705165
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1715	0.0	69.68744	1
GTATCAA	2880	0.0	49.79748	1
GTGGTAT	730	0.0	40.110847	1
GTACATG	6345	0.0	37.106777	1
TACATGG	6690	0.0	34.924034	2
ACATGGG	6840	0.0	33.98388	3
TCAACGC	4005	0.0	33.782192	4
TATCAAC	4255	0.0	33.478252	2
TGGTATC	830	0.0	33.03271	2
CATGGGG	5935	0.0	32.738693	4
CAACGCA	4095	0.0	32.603077	5
ATCAACG	4405	0.0	32.06763	3
AACGCAG	4230	0.0	31.418985	6
ATGGGGG	3760	0.0	29.484213	5
GTAAGTA	475	0.0	26.350578	2
GTATAGG	250	6.675691E-10	26.29307	1
ACGCAGA	5250	0.0	24.513859	7
CGCAGAG	5460	0.0	23.45593	8
GTACACT	970	0.0	23.34943	4
TACACTG	1330	0.0	22.406952	5
>>END_MODULE