FastQCFastQC Report
Mon 15 Aug 2016
SRR2921307_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921307_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893429
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10700.11976329400545538No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8980.10051162431485881No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.066.3684841
GTATCAA20100.053.8945851
GTACATG65500.038.5295871
TCAACGC27700.038.028984
CAACGCA28150.037.6324775
TACATGG66250.036.833542
TATCAAC29300.036.7668532
ATCAACG29450.035.9732933
AACGCAG29650.035.321266
ACATGGG69600.034.8896333
CATGGGG59000.032.178064
ATGGGGG38050.031.2821455
ACGCAGA36900.028.213877
GTAAGTA7500.027.7748852
CGCAGAG38500.026.8853198
TGGGGGG30800.026.2746166
ATATGTA5000.024.9987961
AGTACAT45350.024.6732312
GCAGAGT41100.024.6055899
GAGTACA47600.024.3835811
Produced by FastQC (version 0.11.5)