##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921307_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893429
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.927620437662085	31.0	31.0	34.0	26.0	34.0
2	30.071860214969515	31.0	31.0	34.0	26.0	34.0
3	30.14109683030213	33.0	31.0	34.0	25.0	34.0
4	33.527202497344504	37.0	35.0	37.0	28.0	37.0
5	33.53556466154557	37.0	35.0	37.0	28.0	37.0
6	33.64781532723921	37.0	35.0	37.0	28.0	37.0
7	33.580233012360246	37.0	35.0	37.0	28.0	37.0
8	33.599287688221445	37.0	35.0	37.0	28.0	37.0
9	35.06954106034167	39.0	37.0	39.0	27.0	39.0
10-11	35.0845394541704	39.0	37.0	39.0	27.0	39.0
12-13	34.97811577640753	39.0	37.0	39.0	27.0	39.0
14-15	36.199127183021815	40.0	37.0	41.0	27.0	41.0
16-17	36.176292128417586	40.0	37.0	41.0	27.0	41.0
18-19	36.17236400430252	40.0	37.0	41.0	27.0	41.0
20-21	36.20498439159687	40.0	37.0	41.0	27.0	41.0
22-23	36.164300129053345	40.0	37.0	41.0	27.0	41.0
24-25	36.162212666031664	40.0	37.0	41.0	27.0	41.0
26-27	36.134461160316036	40.0	37.0	41.0	27.0	41.0
28-29	36.060718311136085	40.0	37.0	41.0	27.0	41.0
30-31	35.97509706982872	40.0	36.0	41.0	27.0	41.0
32-33	35.870906921534896	40.0	36.0	41.0	27.0	41.0
34-35	35.745426888986145	40.0	36.0	41.0	26.0	41.0
36-37	35.627832206028685	40.0	36.0	41.0	25.0	41.0
38-39	35.5148360977761	40.0	35.0	41.0	25.0	41.0
40-41	35.39237029467367	40.0	35.0	41.0	24.5	41.0
42-43	35.18805467474192	40.0	35.0	41.0	24.0	41.0
44-45	34.944288242266595	40.0	35.0	41.0	23.0	41.0
46-47	34.70953987390156	39.5	35.0	41.0	22.5	41.0
48-49	34.496353375590004	39.0	35.0	41.0	21.0	41.0
50-51	34.310587075190085	39.0	34.0	41.0	20.0	41.0
52-53	34.10959852433713	39.0	34.0	41.0	18.5	41.0
54-55	33.896333676207064	38.0	34.0	40.0	17.5	41.0
56-57	33.65177031414919	38.0	34.0	40.0	14.5	41.0
58-59	33.37302348591774	37.5	33.0	40.0	12.5	41.0
60-61	33.067490533663	37.0	33.0	40.0	8.5	41.0
62-63	32.72551987902788	36.5	33.0	40.0	7.5	41.0
64-65	32.385303141044226	36.0	32.5	39.0	6.5	41.0
66-67	32.04339180841455	35.5	32.0	39.0	2.0	40.5
68-69	31.68401350303158	35.0	32.0	38.5	2.0	40.0
70-71	31.62909755559759	35.0	32.0	37.5	2.0	40.0
72-73	31.334701470402237	35.0	32.0	37.0	2.0	39.0
74-75	31.009940913044012	35.0	32.0	37.0	2.0	39.0
76-77	30.717791788715164	35.0	32.0	36.0	2.0	38.5
78-79	30.438280490111694	35.0	31.5	36.0	2.0	37.0
80-81	30.16113087889469	35.0	31.0	35.0	2.0	37.0
82-83	29.942430232284828	35.0	31.0	35.0	2.0	36.5
84-85	29.73628570373247	35.0	31.0	35.0	2.0	36.0
86-87	29.570346384547626	35.0	31.0	35.0	2.0	36.0
88-89	29.424630272802876	35.0	31.0	35.0	2.0	35.5
90-91	29.29283636416548	35.0	31.0	35.0	2.0	35.0
92-93	29.172372398925937	34.0	30.5	35.0	2.0	35.0
94-95	29.03898575040658	34.0	30.0	35.0	2.0	35.0
96-97	28.94199818899991	34.0	30.0	35.0	2.0	35.0
98-99	28.851990477139204	34.0	30.0	35.0	2.0	35.0
100-101	28.74639450924472	34.0	30.0	35.0	2.0	35.0
102-103	27.911464705085685	33.5	28.0	34.5	2.0	35.0
104-105	28.49868875982311	34.0	29.0	35.0	2.0	35.0
106-107	28.567651150790944	34.0	29.0	35.0	2.0	35.0
108-109	28.493185804356024	34.0	29.0	35.0	2.0	35.0
110-111	28.399586872599837	34.0	29.0	35.0	2.0	35.0
112-113	28.300982506724093	34.0	29.0	35.0	2.0	35.0
114-115	28.152643354984	34.0	29.0	35.0	2.0	35.0
116-117	28.01042276442784	34.0	29.0	35.0	2.0	35.0
118-119	28.00275007862964	34.0	29.0	35.0	2.0	35.0
120-121	28.171185958817098	34.0	29.5	35.0	2.0	35.0
122-123	28.010218495258158	34.0	29.0	35.0	2.0	35.0
124-125	27.39990642793104	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37080.0
3	5964.0
4	4038.0
5	3450.0
6	3629.0
7	4007.0
8	3990.0
9	3978.0
10	4523.0
11	4792.0
12	4889.0
13	4752.0
14	4962.0
15	4314.0
16	3589.0
17	3275.0
18	3037.0
19	2811.0
20	2810.0
21	2838.0
22	3198.0
23	4389.0
24	6229.0
25	7593.0
26	7788.0
27	8672.0
28	10349.0
29	12477.0
30	15130.0
31	18790.0
32	24373.0
33	33803.0
34	53898.0
35	85968.0
36	166924.0
37	261101.0
38	60000.0
39	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.65712049955612	17.90874682208015	12.493940822122688	24.940191856241036
2	16.488791275710213	20.149509359447098	38.73440931713313	24.62729004770956
3	20.17196448321102	24.940782963404097	27.805477072148516	27.08177548123637
4	13.803251748564879	15.493941592111979	33.85118615835147	36.85162050097167
5	14.315090037398514	39.374557144951986	31.20956936299394	15.100783454655561
6	33.469182108268484	33.83854895594245	17.437944354251805	15.254324581537256
7	29.720955693446427	33.43371032543415	18.82191740951238	18.023416571607044
8	27.747726972513213	35.314596855262835	18.743045465027507	18.194630707196445
9	28.859463834160003	16.94354799892091	17.69600254325606	36.50098562366303
10-11	26.63492477485066	26.6213802741698	25.426050104578096	21.317644846401446
12-13	26.263856994861573	24.245984150961558	25.849357458557044	23.640801395619825
14-15	24.225923929742553	25.316959406025376	23.368945617808492	27.088171046423575
16-17	24.247791969097364	28.039152147474805	24.072895661620137	23.640160221807694
18-19	24.344294270176004	27.46556715894151	25.575475768984724	22.614662801897758
20-21	25.48936425042689	25.807650396780314	25.512366818767184	23.19061853402561
22-23	25.504125348816125	25.180858291945523	25.282271018235264	24.03274534100309
24-25	24.897048403760547	25.403047523956744	25.33096181808209	24.368942254200615
26-27	24.15886446072574	25.894611594817718	25.65697754789199	24.28954639656455
28-29	24.213377122286698	26.616620549466298	25.242283204384268	23.92771912386274
30-31	25.387573037229398	25.685990284089637	25.190176632563972	23.736260046116993
32-33	24.88634118520832	25.690502136052523	25.159581285571726	24.26357539316743
34-35	24.72824353140689	26.109777188239836	25.478673091883962	23.68330618846931
36-37	24.459682727309364	26.3200106563506	25.499572402738412	23.72073421360162
38-39	24.791896718759883	26.32200782097829	25.286318660930313	23.59977679933152
40-41	24.686186145848723	25.929968075722776	25.322153120116774	24.061692658311728
42-43	24.188754073789674	26.004420373118	25.103779144771416	24.703046408320915
44-45	24.063915823643818	26.049671885712687	25.54243098406302	24.343981306580474
46-47	23.97274496426051	25.917750194634852	25.43780947780771	24.671695363296934
48-49	23.833635752048743	26.300353835493922	25.561620098662008	24.30439031379533
50-51	24.149560683541598	26.019097211533992	25.670252127742334	24.161089977182073
52-53	24.188755065033916	25.978251136134677	25.510085294051805	24.3229085047796
54-55	23.545451492662615	25.681128759640465	25.933879579569496	24.839540168127428
56-57	24.012362365975957	25.935390854602836	25.185578209818814	24.866668569602393
58-59	24.04798044246242	26.25469573432554	25.444611602885274	24.252712220326764
60-61	24.727972822467358	25.925272978615045	25.465168992091876	23.881585206825726
62-63	24.341881394255523	25.844153663614588	25.54909445028991	24.26487049183998
64-65	23.918889498919015	25.493103021775838	25.6949726241985	24.893034855106652
66-67	24.124279082262277	25.92119365228609	25.231687080426575	24.722840185025056
68-69	23.75762561984396	25.974288368760984	25.520501024211693	24.747584987183362
70-71	23.762450490464115	25.735220455602466	25.789004447673104	24.713324606260322
72-73	23.822979786532077	26.328381965536867	25.26742255197761	24.581215695953446
74-75	23.639053072298967	26.20824283113451	25.51224571004187	24.640458386524656
76-77	23.507314834506765	25.582780196778565	25.580373633015817	25.329531335698857
78-79	23.62004392219369	25.70136713984137	25.79908394690832	24.879504991056617
80-81	23.860745856430892	26.053766359507613	25.43814238263269	24.647345401428808
82-83	23.863814440375023	26.050332219963646	25.67154773311364	24.414305606547686
84-85	23.90025074439743	26.072380056865246	25.264848769785296	24.762520428952023
86-87	23.33771006345699	26.46987052266665	25.459248836696474	24.733170577179887
88-89	23.435605002750762	25.851239410402084	25.675280127290645	25.037875459556506
90-91	23.962412516475307	25.417052606246482	25.841730725641032	24.778804151637175
92-93	24.20307124351389	25.51504083219203	25.643713588660233	24.63817433563385
94-95	23.934884434198544	25.638330560112294	25.539602771765868	24.887182233923294
96-97	23.402469050120363	25.557081042537575	25.729859700595348	25.31059020674671
98-99	23.09564226511993	25.63083083015854	25.682937162896934	25.590589741824594
100-101	23.161246991660605	26.046566295404936	25.740191414339286	25.051995298595177
102-103	22.98190353702068	26.14472693000395	25.702809222065888	25.17056031090948
104-105	23.118105952785378	26.092176024204477	25.631234339819684	25.158483683190457
106-107	23.19426705605672	25.935370056740652	25.79942307886044	25.070939808342185
108-109	23.064666069300785	26.009594046788187	25.81068352126479	25.115056362646236
110-111	22.766885933859406	26.720187786595357	25.651295202520387	24.861631077024853
112-113	22.61763379140283	26.853647971148003	25.70979877701377	24.8189194604354
114-115	22.74050095925033	26.961034512864252	25.516282630741234	24.782181897144188
116-117	22.50698873128293	26.69116103883748	25.68405617848618	25.11779405139341
118-119	22.533948826610835	26.374952002174968	25.87627912451967	25.214820046694523
120-121	22.42841207198504	26.513601720315517	26.083802694317686	24.974183513381757
122-123	22.45427916155051	26.69331360370535	25.780295966805873	25.072111267938276
124-125	22.210911060879358	26.38708012910021	25.79133201890052	25.610676791119914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	888.0
1	724.0
2	472.0
3	338.0
4	253.0
5	205.0
6	196.0
7	213.5
8	248.0
9	283.5
10	302.0
11	307.5
12	336.5
13	375.5
14	411.5
15	464.0
16	520.0
17	548.0
18	601.5
19	663.5
20	689.0
21	758.0
22	876.0
23	1029.5
24	1147.5
25	1360.0
26	1747.5
27	2231.0
28	2697.5
29	3307.5
30	4222.5
31	5200.0
32	6618.0
33	8260.0
34	9818.5
35	11502.5
36	13342.0
37	15133.5
38	16473.0
39	17757.5
40	19136.5
41	20459.5
42	22069.5
43	24441.0
44	26870.5
45	29413.5
46	31841.0
47	34325.0
48	37579.5
49	40327.5
50	41791.5
51	42305.5
52	41857.0
53	40932.0
54	40213.5
55	38953.5
56	36243.0
57	32787.0
58	29310.5
59	25764.0
60	22030.5
61	18447.5
62	15538.5
63	12214.0
64	8941.0
65	6646.5
66	5019.5
67	3840.5
68	2937.5
69	2273.0
70	1773.0
71	1301.5
72	973.0
73	806.5
74	620.5
75	447.0
76	302.5
77	194.5
78	126.5
79	83.5
80	51.0
81	28.5
82	23.0
83	22.5
84	15.0
85	8.0
86	9.0
87	8.0
88	9.5
89	10.0
90	7.5
91	4.5
92	1.5
93	1.5
94	1.5
95	1.5
96	2.0
97	2.0
98	1.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017460816696122466
2	0.011976329400545539
3	0.011752472776236277
4	0.013991039019328902
5	0.008730408348061233
6	0.031227999091142107
7	0.014550680580102055
8	0.014214895643638162
9	0.009625834845298283
10-11	0.008674444191983918
12-13	0.006995519509664451
14-15	0.006435877948891295
16-17	0.004756953266571826
18-19	0.006659734573200556
20-21	0.005652379763808875
22-23	0.00559641560773156
24-25	0.005372558983422298
26-27	0.004309240017953301
28-29	0.005932200544195454
30-31	0.005484487295576929
32-33	0.008450587567674656
34-35	0.008282695099442709
36-37	0.007275340290051027
38-39	0.006995519509664451
40-41	0.007275340290051027
42-43	0.007331304446128343
44-45	0.007443232758282976
46-47	0.010017583937839492
48-49	0.008506551723751971
50-51	0.005876236388118138
52-53	0.005484487295576929
54-55	0.006603770417123241
56-57	0.008114802631210762
58-59	0.007275340290051027
60-61	0.004924845734803773
62-63	0.005484487295576929
64-65	0.0034138135207162513
66-67	0.004085383393644039
68-69	0.006603770417123241
70-71	0.004533096642262564
72-73	0.004421168330107933
74-75	0.003861526769334776
76-77	0.0043652041740306164
78-79	0.0034697776767935676
80-81	0.002966100272097727
82-83	0.005036774046958404
84-85	0.009961619781762177
86-87	0.007611125226514921
88-89	0.004533096642262564
90-91	0.005204666515190351
92-93	0.008674444191983918
94-95	0.007107447821819081
96-97	0.010017583937839492
98-99	0.007107447821819081
100-101	0.008842336660215866
102-103	0.00559641560773156
104-105	0.004533096642262564
106-107	0.00738726860220566
108-109	0.006995519509664451
110-111	0.008338659255520025
112-113	0.00470098911049451
114-115	0.0025743511795565176
116-117	0.002966100272097727
118-119	0.1635832282139935
120-121	0.01169650862015896
122-123	0.0020147096187833617
124-125	0.0016229605262421526
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	893429.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.12799286403084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.98626625630095	56.37829481929565
2	8.85080665487257	11.882737719384053
3	2.780142323215661	5.59876122101433
4	1.3060759393885286	3.5069702535662217
5	0.768449133705391	2.5792223981873086
6	0.48791032247926663	1.965146438740512
7	0.3605633312242282	1.6942724907815825
8	0.2454764194641368	1.3182671467261133
9	0.18955134875320326	1.1451781427825205
>10	0.9832679658056223	11.289805823316723
>50	0.0336602393476897	1.5193199643714594
>100	0.007330274085365715	0.8275664970273747
>500	3.331942382655674E-4	0.1747954514060216
>1k	1.665971191327837E-4	0.11966163340015554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1070	0.11976329400545538	No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	898	0.10051162431485881	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.1192831215463121E-4
6	0.0	0.0	0.0	0.0	1.1192831215463121E-4
7	0.0	0.0	0.0	0.0	1.1192831215463121E-4
8	0.0	0.0	0.0	0.0	1.1192831215463121E-4
9	0.0	0.0	0.0	0.0	1.1192831215463121E-4
10-11	0.0	0.0	0.0	0.0	1.1192831215463121E-4
12-13	0.0	0.0	0.0	0.0	2.2385662430926241E-4
14-15	0.0	0.0	0.0	0.0	2.2385662430926241E-4
16-17	0.0	0.0	0.0	5.5964156077315604E-5	3.357849364638936E-4
18-19	0.0	0.0	0.0	1.1192831215463121E-4	5.59641560773156E-4
20-21	0.0	0.0	0.0	1.1192831215463121E-4	5.59641560773156E-4
22-23	0.0	0.0	0.0	3.357849364638936E-4	5.59641560773156E-4
24-25	0.0	5.5964156077315604E-5	0.0	0.001287175589778259	5.59641560773156E-4
26-27	0.0	1.1192831215463121E-4	0.0	0.002686279491711149	5.59641560773156E-4
28-29	0.0	1.1192831215463121E-4	0.0	0.007163411977896397	5.59641560773156E-4
30-31	0.0	1.1192831215463121E-4	0.0	0.02848575544335364	5.59641560773156E-4
32-33	0.0	1.1192831215463121E-4	0.0	0.06592577585907777	5.59641560773156E-4
34-35	0.0	1.678924682319468E-4	0.0	0.11248795371540435	5.59641560773156E-4
36-37	0.0	2.2385662430926241E-4	0.0	0.1785816220427141	5.59641560773156E-4
38-39	0.0	2.2385662430926241E-4	0.0	0.28743190561309295	6.156057168504716E-4
40-41	0.0	2.2385662430926241E-4	0.0	0.4052924183119196	6.715698729277872E-4
42-43	0.0	2.2385662430926241E-4	0.0	0.5148702359113035	6.715698729277872E-4
44-45	0.0	2.2385662430926241E-4	0.0	0.6463300385369178	6.715698729277872E-4
46-47	0.0	2.2385662430926241E-4	0.0	0.7821550453365629	6.715698729277872E-4
48-49	0.0	2.2385662430926241E-4	0.0	0.9285572776348204	6.715698729277872E-4
50-51	0.0	2.2385662430926241E-4	0.0	1.0704264132908154	6.715698729277872E-4
52-53	0.0	2.2385662430926241E-4	0.0	1.2148139359702896	7.275340290051028E-4
54-55	0.0	2.2385662430926241E-4	0.0	1.3776136659992009	7.834981850824185E-4
56-57	0.0	2.2385662430926241E-4	0.0	1.5602806714355588	7.834981850824185E-4
58-59	0.0	2.2385662430926241E-4	0.0	1.7541405080873802	7.834981850824185E-4
60-61	0.0	2.2385662430926241E-4	0.0	1.9460975634325726	7.834981850824185E-4
62-63	0.0	2.2385662430926241E-4	0.0	2.1489676292128417	7.834981850824185E-4
64-65	0.0	2.2385662430926241E-4	0.0	2.356482719947528	7.834981850824185E-4
66-67	0.0	3.357849364638936E-4	0.0	2.594666168212583	7.834981850824185E-4
68-69	0.0	3.357849364638936E-4	0.0	2.8463369780922716	7.834981850824185E-4
70-71	0.0	3.357849364638936E-4	0.0	3.1205613428711176	7.834981850824185E-4
72-73	0.0	3.357849364638936E-4	0.0	3.3841525179952745	7.834981850824185E-4
74-75	0.0	3.357849364638936E-4	0.0	3.6547392126290954	7.834981850824185E-4
76-77	0.0	3.357849364638936E-4	0.0	3.9828010955543194	7.834981850824185E-4
78-79	0.0	3.357849364638936E-4	0.0	4.340859766136985	8.954264972370497E-4
80-81	0.0	3.357849364638936E-4	0.0	4.6799465878094395	9.513906533143653E-4
82-83	0.0	3.357849364638936E-4	0.0	5.025301394962554	0.0013431397458555745
84-85	0.0	3.357849364638936E-4	0.0	5.380058180336658	0.0013431397458555745
86-87	0.0	3.357849364638936E-4	0.0	5.797886569609896	0.0013431397458555745
88-89	0.0	3.357849364638936E-4	0.0	6.26854512222012	0.0013431397458555745
90-91	0.0	3.357849364638936E-4	0.0	6.748437760583101	0.0013431397458555745
92-93	0.0	3.357849364638936E-4	0.0	7.2071759479488575	0.0014550680580102057
94-95	0.0	3.357849364638936E-4	0.0	7.681416206548031	0.001566996370164837
96-97	0.0	3.357849364638936E-4	0.0	8.220630850352965	0.0016229605262421524
98-99	0.0	5.036774046958404E-4	0.0	8.828345621196537	0.0016789246823194681
100-101	0.0	5.59641560773156E-4	0.0	9.445686226885403	0.0016789246823194681
102-103	0.0	5.59641560773156E-4	0.0	10.060956158799412	0.0016789246823194681
104-105	0.0	5.59641560773156E-4	0.0	10.723124053506211	0.0016789246823194681
106-107	0.0	5.59641560773156E-4	0.0	11.45049019004308	0.0016789246823194681
108-109	0.0	5.59641560773156E-4	0.0	12.220109264418326	0.0017908529944740993
110-111	0.0	5.59641560773156E-4	0.0	13.01099471810295	0.0019587454627060462
112-113	0.0	5.59641560773156E-4	0.0	13.794996580590063	0.0020147096187833617
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1130	0.0	66.368484	1
GTATCAA	2010	0.0	53.894585	1
GTACATG	6550	0.0	38.529587	1
TCAACGC	2770	0.0	38.02898	4
CAACGCA	2815	0.0	37.632477	5
TACATGG	6625	0.0	36.83354	2
TATCAAC	2930	0.0	36.766853	2
ATCAACG	2945	0.0	35.973293	3
AACGCAG	2965	0.0	35.32126	6
ACATGGG	6960	0.0	34.889633	3
CATGGGG	5900	0.0	32.17806	4
ATGGGGG	3805	0.0	31.282145	5
ACGCAGA	3690	0.0	28.21387	7
GTAAGTA	750	0.0	27.774885	2
CGCAGAG	3850	0.0	26.885319	8
TGGGGGG	3080	0.0	26.274616	6
ATATGTA	500	0.0	24.998796	1
AGTACAT	4535	0.0	24.673231	2
GCAGAGT	4110	0.0	24.605589	9
GAGTACA	4760	0.0	24.383581	1
>>END_MODULE