FastQCFastQC Report
Mon 15 Aug 2016
SRR2921307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893429
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.2850814110578457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.2341540290274885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.21020137022639737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.075.6926351
GTATCAA36750.055.7962841
GTAAGTA4650.041.0178532
TATCAAC49200.040.5840722
TCAACGC49100.039.5741924
ATCAACG50450.039.105943
CAACGCA49750.038.8175245
GTACATG66050.038.557181
TACATGG67000.038.1643942
AACGCAG51500.037.4732676
ACATGGG71500.035.7624443
ATGGGGG36800.035.47095
GTGGTAT7600.035.397081
TGGTATC7800.035.151122
CATGGGG60450.034.5102164
TGTAAGT5600.030.9583971
ACGCAGA62800.030.625327
TGGGGGG29900.030.2800246
CGCAGAG64650.029.6568578
TAAGTAC8200.029.0751553
Produced by FastQC (version 0.11.5)