##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921307_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893429
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.94613226120934	31.0	30.0	34.0	16.0	34.0
2	29.75247501480252	33.0	31.0	34.0	25.0	34.0
3	29.996051169147183	33.0	31.0	34.0	26.0	34.0
4	32.03052061215833	37.0	33.0	37.0	19.0	37.0
5	31.715801703325056	37.0	33.0	37.0	19.0	37.0
6	31.89336253916092	37.0	33.0	37.0	17.0	37.0
7	32.12783892172741	37.0	35.0	37.0	17.0	37.0
8	32.38247135474671	37.0	35.0	37.0	17.0	37.0
9	34.101093651538065	39.0	35.0	39.0	17.0	39.0
10-11	34.31490023269896	39.0	37.0	39.0	17.5	39.0
12-13	34.35589845415808	39.0	37.0	39.0	19.5	39.0
14-15	35.689755425445114	40.0	37.0	41.0	22.0	41.0
16-17	35.72944464529358	40.0	37.5	41.0	23.0	41.0
18-19	35.77655751044571	40.5	38.0	41.0	23.0	41.0
20-21	35.77198020212015	41.0	38.0	41.0	23.0	41.0
22-23	35.729604702779966	40.5	37.5	41.0	22.5	41.0
24-25	35.66161776705256	41.0	37.0	41.0	22.0	41.0
26-27	35.547315455397126	40.0	37.0	41.0	20.0	41.0
28-29	35.45818302293747	40.0	37.0	41.0	18.5	41.0
30-31	35.32103614277128	40.0	36.5	41.0	18.0	41.0
32-33	35.14293581247083	40.0	35.5	41.0	16.0	41.0
34-35	35.04050293867783	40.0	35.0	41.0	15.5	41.0
36-37	34.94829023906768	40.0	35.0	41.0	14.0	41.0
38-39	34.83346466255293	40.0	35.0	41.0	9.0	41.0
40-41	34.664626959724835	40.0	35.0	41.0	7.5	41.0
42-43	34.47921099494196	40.0	35.0	41.0	2.0	41.0
44-45	34.266904253163936	40.0	35.0	41.0	2.0	41.0
46-47	33.95515592173524	40.0	35.0	41.0	2.0	41.0
48-49	33.65861976721149	40.0	35.0	41.0	2.0	41.0
50-51	33.455805665587306	39.0	34.5	41.0	2.0	41.0
52-53	33.26884061296421	39.0	34.0	41.0	2.0	41.0
54-55	33.07317984976982	39.0	34.0	41.0	2.0	41.0
56-57	32.83689806352827	38.0	34.0	41.0	2.0	41.0
58-59	32.7644194446341	38.0	34.0	41.0	2.0	41.0
60-61	32.52257761948627	37.0	34.0	40.5	2.0	41.0
62-63	32.22986549574729	37.0	33.5	40.0	2.0	41.0
64-65	31.9403847423802	36.0	33.0	39.5	2.0	41.0
66-67	31.63053079763473	36.0	33.0	39.0	2.0	41.0
68-69	31.295755454546473	35.0	33.0	39.0	2.0	41.0
70-71	30.973706360550196	35.0	33.0	37.5	2.0	40.5
72-73	30.63723530353279	35.0	32.0	37.0	2.0	39.0
74-75	30.3118423512109	35.0	32.0	37.0	2.0	39.0
76-77	30.02568195122388	35.0	32.0	36.0	2.0	39.0
78-79	29.77802545025962	35.0	32.0	36.0	2.0	37.0
80-81	29.55807904153548	35.0	32.0	35.0	2.0	37.0
82-83	29.36392651234737	35.0	31.0	35.0	2.0	36.5
84-85	29.204565779709412	35.0	31.0	35.0	2.0	36.0
86-87	29.067488854738315	35.0	31.0	35.0	2.0	36.0
88-89	28.9475951642492	35.0	31.0	35.0	2.0	36.0
90-91	28.845509268223886	35.0	31.0	35.0	2.0	35.0
92-93	28.780271291843	35.0	31.0	35.0	2.0	35.0
94-95	28.706643169183003	35.0	31.0	35.0	2.0	35.0
96-97	28.631874497022146	35.0	31.0	35.0	2.0	35.0
98-99	28.58201770929755	35.0	31.0	35.0	2.0	35.0
100-101	28.52931570387798	35.0	30.5	35.0	2.0	35.0
102-103	27.866721921943434	34.0	29.0	35.0	2.0	35.0
104-105	28.39211901561288	35.0	30.0	35.0	2.0	35.0
106-107	28.43687299158635	35.0	30.0	35.0	2.0	35.0
108-109	28.50116573337109	35.0	30.5	35.0	2.0	35.0
110-111	28.60208309781751	35.0	31.0	35.0	2.0	35.0
112-113	28.556554577923933	35.0	31.0	35.0	2.0	35.0
114-115	28.479043662115288	35.0	31.0	35.0	2.0	35.0
116-117	28.401127565816644	35.0	31.0	35.0	2.0	35.0
118-119	28.30323898149713	35.0	31.0	35.0	2.0	35.0
120-121	28.188303715236465	35.0	31.0	35.0	2.0	35.0
122-123	28.052728868214487	35.0	31.0	35.0	2.0	35.0
124-125	27.527851121913436	34.5	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64588.0
3	3732.0
4	2948.0
5	2934.0
6	3090.0
7	3202.0
8	3456.0
9	3461.0
10	3689.0
11	3855.0
12	4614.0
13	6298.0
14	8028.0
15	6666.0
16	3677.0
17	3158.0
18	2778.0
19	2384.0
20	2333.0
21	2331.0
22	2557.0
23	3092.0
24	4119.0
25	5203.0
26	5996.0
27	6956.0
28	8030.0
29	9669.0
30	11880.0
31	14489.0
32	18818.0
33	25628.0
34	39455.0
35	67553.0
36	139640.0
37	294427.0
38	98573.0
39	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.898011906099065	17.844659103672917	12.30821071548916	25.949118274738854
2	16.419995321396552	20.08508790289995	38.0724153793978	25.422501396305698
3	19.4373587604611	24.26337179563233	28.591191913403303	27.70807753050326
4	14.294174477950964	16.203922938363903	30.672626864101005	38.829275719584125
5	14.33700759147333	38.54461252088496	31.168100113255363	15.950279774386347
6	33.43072183581619	34.01740450868825	16.517485696880247	16.034387958615298
7	29.959248105148845	32.92484410161104	18.10201408403399	19.013893709206123
8	28.645355846371352	34.65151917111902	17.541072973720237	19.1620520087894
9	29.35129247862846	15.998907496521564	17.554605584976287	37.095194439873694
10-11	27.411165808154415	25.768295713259143	24.44845904325693	22.372079435329514
12-13	27.282450202817266	23.535426386627275	25.668759421315634	23.51336398923982
14-15	25.178329783340363	24.320679091455506	23.8016115438384	26.69937958136573
16-17	24.21826468583402	27.282526087691355	24.464506972574206	24.034702253900424
18-19	24.19447991950116	25.990537580490447	27.03555626692216	22.779426233086234
20-21	25.24459134413591	24.52382897801616	27.232941845406856	22.99863783244108
22-23	25.052410432166404	23.92926578385076	27.0080778662882	24.010245917694633
24-25	24.48711649162944	24.541065938087975	26.85154612173995	24.12027144854264
26-27	23.79808580200553	24.876906838707942	27.381582643948203	23.94342471533832
28-29	23.84940493312843	25.419591260189673	27.042719678900056	23.688284127781838
30-31	24.902930171283895	24.700004141347552	26.902417539614227	23.494648147754326
32-33	24.34351246713505	24.771246512033972	26.96767174560038	23.917569275230598
34-35	24.426171525661246	25.04703787318298	27.288626180703783	23.23816442045199
36-37	24.269415924488683	25.06774461093159	27.32393956318857	23.33889990139116
38-39	24.27641144399835	25.27318902789142	27.025538682984322	23.424860845125913
40-41	24.252738605977644	25.01765669124239	26.994198755581028	23.735405947198938
42-43	24.003977932213978	25.011724490698196	26.56584910496525	24.418448472122574
44-45	23.750180484403348	25.228809452122103	26.944894334076913	24.076115729397635
46-47	23.84442412323755	25.17301318851302	26.333206108151852	24.649356580097578
48-49	23.95489736733417	25.726442727961597	25.98107963811338	24.337580266590855
50-51	24.090946230758124	25.512155974341553	26.17980835634393	24.217089438556393
52-53	24.31122114907844	25.44091360365513	25.988522870871666	24.259342376394766
54-55	23.561133565174178	25.060525234797616	26.517160289177987	24.86118091085022
56-57	23.82416509873756	25.35327373523805	25.789570296016805	25.032990870007577
58-59	23.996086986207075	25.47846555238301	26.196093925762426	24.32935353564749
60-61	24.648013440351725	25.168088342778216	26.215121738828717	23.968776478041345
62-63	24.123685262063354	25.132047426264425	26.30930941350684	24.434957898165383
64-65	23.791817816524873	24.764922562397235	26.42498732411865	25.018272296959243
66-67	23.90318648711873	25.390433934873393	25.986004483848184	24.720375094159692
68-69	23.650116573337108	25.27083853333617	26.180647818685088	24.898397074641636
70-71	23.743017072425452	25.12577944078377	26.274611636738904	24.856591850051878
72-73	23.76187699302351	25.757782655364892	25.725771158088666	24.754569193522933
74-75	23.518041164994642	25.616025448021052	25.958638011526375	24.907295375457927
76-77	23.4347665007516	24.970926620917837	25.999323952994587	25.59498292533598
78-79	23.47181477207478	25.153817482978504	26.106271455258334	25.26809628968838
80-81	23.779505702187862	25.488818921257312	25.72006281416878	25.01161256238604
82-83	23.695279647291503	25.49704565220068	25.950523208895167	24.857151491612655
84-85	23.65425792088683	25.582726775155052	25.555808016081862	25.20720728787626
86-87	23.134462839240726	25.857622709806822	25.65654349702103	25.351370953931422
88-89	23.054434096050162	25.500179644941007	25.907598701183865	25.537787557824963
90-91	23.522123240063262	25.011892409788377	25.971791763624786	25.494192586523578
92-93	23.73035020827142	25.22611006737551	25.758331276642544	25.285208447710527
94-95	23.528364904614584	25.242492959701284	25.62534417994511	25.603797955739015
96-97	23.09862433969033	25.11462903174355	25.753910090992477	26.032836537573644
98-99	22.7698131411747	25.131949562835747	25.745876698583043	26.35236059740651
100-101	22.92017608562068	25.459997380877496	25.81324313403751	25.806583399464312
102-103	22.820224102866597	25.437275933510108	25.668240005641184	26.07425995798211
104-105	22.931101929253433	25.326201257291437	25.648890761823694	26.09380605163144
106-107	22.97739717134453	25.126702991175563	25.797378188281538	26.098521649198368
108-109	22.88360920972146	25.153860058840944	25.76812504232306	26.194405689114536
110-111	22.667182284822694	25.846404738612932	25.608780134001023	25.877632842563347
112-113	22.471196406175583	25.992490666499517	25.649315580607812	25.886997346717088
114-115	22.4651943948383	25.99676638201352	25.49722892705779	26.040810296090388
116-117	22.27644560502382	25.709923681210533	25.544716475445295	26.46891423832035
118-119	22.244178875274645	25.45143587556678	25.839436238215324	26.464949010943258
120-121	22.262087445308907	25.614347898597202	25.899038197860598	26.2245264582333
122-123	22.12172106582465	25.874527023661546	25.50621969831371	26.49753221220009
124-125	21.780222793679396	25.700294651776446	25.41247051383576	27.107012040708394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50.0
1	42.0
2	63.0
3	75.5
4	57.5
5	87.0
6	210.5
7	591.0
8	892.5
9	964.0
10	862.5
11	685.5
12	666.0
13	675.0
14	721.0
15	799.0
16	847.5
17	915.5
18	1005.0
19	1074.0
20	1138.0
21	1165.5
22	1223.5
23	1337.0
24	1469.0
25	1666.0
26	2010.0
27	2463.5
28	2843.0
29	3311.5
30	4032.0
31	4946.0
32	6306.0
33	7850.0
34	9279.0
35	10717.5
36	12496.5
37	14317.0
38	15374.0
39	16771.5
40	18505.5
41	19808.0
42	21111.0
43	23308.0
44	25943.0
45	28268.0
46	30671.5
47	33416.0
48	36758.5
49	39838.0
50	41985.5
51	43057.5
52	42396.0
53	41420.5
54	40738.5
55	39201.0
56	36592.5
57	33238.0
58	29953.5
59	26410.0
60	22470.0
61	18780.0
62	15766.0
63	12526.0
64	9194.0
65	6855.0
66	5243.0
67	4039.5
68	3102.0
69	2354.0
70	1811.5
71	1379.0
72	1090.0
73	940.0
74	711.0
75	498.5
76	362.5
77	240.5
78	184.0
79	143.5
80	115.5
81	102.0
82	90.0
83	80.5
84	83.0
85	84.0
86	81.0
87	71.0
88	63.5
89	59.0
90	48.5
91	43.5
92	42.5
93	40.0
94	37.0
95	30.5
96	25.0
97	20.5
98	17.0
99	8.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.350223688731841
2	0.0
3	0.0
4	0.019027813066287303
5	0.18345050362144052
6	0.049472313972347
7	0.02451230036186423
8	0.008954264972370497
9	0.007611125226514921
10-11	0.0025743511795565176
12-13	0.056747654262398015
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.238566243092624E-4
92-93	3.9174909254120917E-4
94-95	1.119283121546312E-4
96-97	3.9174909254120917E-4
98-99	5.59641560773156E-5
100-101	0.0
102-103	0.0
104-105	5.59641560773156E-5
106-107	1.119283121546312E-4
108-109	3.9174909254120917E-4
110-111	3.357849364638936E-4
112-113	8.39462341159734E-4
114-115	2.79820780386578E-4
116-117	6.156057168504716E-4
118-119	2.238566243092624E-4
120-121	2.238566243092624E-4
122-123	0.0019587454627060462
124-125	1.678924682319468E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	893429.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.8492179030395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.29096751009692	57.86925439753629
2	8.022931742842143	10.886992880826181
3	2.5682614707307563	5.227635964787753
4	1.2076781115522692	3.2776006138976466
5	0.7314981376313765	2.4815788267909418
6	0.45928543588696885	1.869729457151246
7	0.3257886844308203	1.547315520820391
8	0.2355806372949242	1.278716959484818
9	0.19488500456939073	1.1900515626957096
>10	0.9188739128869311	10.784759901363714
>50	0.03388778074264087	1.5878773360203866
>100	0.009374755083480422	1.0260809944306584
>500	4.934081256850518E-4	0.2451658329670454
>1k	4.934081256850518E-4	0.7272397512272347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2547	0.2850814110578457	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2092	0.2341540290274885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1878	0.21020137022639737	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1192831215463121E-4	0.0
5	0.0	0.0	0.0	1.1192831215463121E-4	0.0
6	0.0	0.0	0.0	1.1192831215463121E-4	0.0
7	0.0	0.0	0.0	1.1192831215463121E-4	0.0
8	0.0	0.0	0.0	1.1192831215463121E-4	0.0
9	0.0	0.0	0.0	1.1192831215463121E-4	1.1192831215463121E-4
10-11	0.0	0.0	0.0	1.1192831215463121E-4	1.1192831215463121E-4
12-13	0.0	0.0	0.0	1.1192831215463121E-4	2.2385662430926241E-4
14-15	0.0	1.1192831215463121E-4	0.0	1.1192831215463121E-4	2.2385662430926241E-4
16-17	0.0	1.1192831215463121E-4	0.0	1.678924682319468E-4	2.2385662430926241E-4
18-19	0.0	1.1192831215463121E-4	0.0	2.2385662430926241E-4	2.2385662430926241E-4
20-21	0.0	1.1192831215463121E-4	0.0	3.357849364638936E-4	2.2385662430926241E-4
22-23	0.0	1.1192831215463121E-4	0.0	6.715698729277872E-4	2.2385662430926241E-4
24-25	0.0	1.1192831215463121E-4	0.0	0.0015110322140875214	2.2385662430926241E-4
26-27	0.0	1.1192831215463121E-4	0.0	0.0029101361160204114	2.2385662430926241E-4
28-29	0.0	1.1192831215463121E-4	0.0	0.007555161070437606	2.2385662430926241E-4
30-31	0.0	1.1192831215463121E-4	0.0	0.027646293102193905	2.79820780386578E-4
32-33	0.0	1.1192831215463121E-4	0.0	0.06284774727482542	3.357849364638936E-4
34-35	0.0	1.1192831215463121E-4	0.0	0.10661171732728622	3.357849364638936E-4
36-37	0.0	1.1192831215463121E-4	0.0	0.16778053991979217	3.357849364638936E-4
38-39	0.0	1.1192831215463121E-4	0.0	0.27036283800951166	3.357849364638936E-4
40-41	0.0	1.1192831215463121E-4	0.0	0.3802204763892822	3.357849364638936E-4
42-43	0.0	1.1192831215463121E-4	0.0	0.4825229536986151	5.036774046958404E-4
44-45	0.0	1.1192831215463121E-4	0.0	0.6054762046004776	6.156057168504716E-4
46-47	0.0	1.1192831215463121E-4	0.0	0.7330185163006797	6.715698729277872E-4
48-49	0.0	1.1192831215463121E-4	0.0	0.8685077381638608	6.715698729277872E-4
50-51	0.0	1.1192831215463121E-4	0.0	1.0010308597549442	6.715698729277872E-4
52-53	0.0	1.1192831215463121E-4	0.0	1.1372476156471303	7.834981850824185E-4
54-55	0.0	1.1192831215463121E-4	0.0	1.2907572957672069	7.834981850824185E-4
56-57	0.0	1.1192831215463121E-4	0.0	1.4627351473927979	7.834981850824185E-4
58-59	0.0	1.1192831215463121E-4	0.0	1.6478645756965578	7.834981850824185E-4
60-61	0.0	1.1192831215463121E-4	0.0	1.8298040471039108	7.834981850824185E-4
62-63	0.0	1.1192831215463121E-4	0.0	2.021928994917335	8.954264972370497E-4
64-65	0.0	1.1192831215463121E-4	0.0	2.2180833619683265	0.0010073548093916809
66-67	0.0	1.1192831215463121E-4	0.0	2.4411564880925063	0.0010073548093916809
68-69	0.0	1.1192831215463121E-4	0.0	2.678892223108943	0.0010073548093916809
70-71	0.0	1.678924682319468E-4	0.0	2.9378943374347597	0.0012312114337009433
72-73	0.0	2.2385662430926241E-4	0.0	3.1855357280768812	0.0013431397458555745
74-75	0.0	2.2385662430926241E-4	0.0	3.441068064725904	0.0013431397458555745
76-77	0.0	2.2385662430926241E-4	0.0	3.749150743931527	0.00139910390193289
78-79	0.0	2.2385662430926241E-4	0.0	4.086950390014204	0.0014550680580102057
80-81	0.0	2.79820780386578E-4	0.0	4.405610294718439	0.0014550680580102057
82-83	0.0	3.357849364638936E-4	0.0	4.730034507498637	0.0014550680580102057
84-85	0.0	3.357849364638936E-4	0.0	5.064476304216676	0.0014550680580102057
86-87	0.0	3.357849364638936E-4	0.0	5.45633732507004	0.0016229605262421524
88-89	0.0	3.357849364638936E-4	0.0	5.895711914433043	0.0016789246823194681
90-91	0.0	3.357849364638936E-4	0.0	6.345048123577811	0.0016789246823194681
92-93	0.0	3.357849364638936E-4	0.0	6.779441903049934	0.0017908529944740993
94-95	0.0	3.357849364638936E-4	0.0	7.229785467004094	0.0019027813066287305
96-97	0.0	3.357849364638936E-4	0.0	7.7437602764181594	0.0019027813066287305
98-99	0.0	3.357849364638936E-4	0.0	8.321925972852906	0.0019027813066287305
100-101	0.0	3.357849364638936E-4	0.0	8.910053289069417	0.0019027813066287305
102-103	0.0	3.357849364638936E-4	0.0	9.492864010458582	0.0021826020870153087
104-105	0.0	3.357849364638936E-4	0.0	10.122572694640535	0.002238566243092624
106-107	0.0	3.357849364638936E-4	0.0	10.811883205044833	0.002238566243092624
108-109	0.0	3.357849364638936E-4	0.0	11.550498136953244	0.0022945303991699396
110-111	0.0	3.357849364638936E-4	0.0	12.30797298945971	0.0024064587113245706
112-113	0.0	3.357849364638936E-4	0.0	13.061754207665075	0.0024624228674018866
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1935	0.0	75.692635	1
GTATCAA	3675	0.0	55.796284	1
GTAAGTA	465	0.0	41.017853	2
TATCAAC	4920	0.0	40.584072	2
TCAACGC	4910	0.0	39.574192	4
ATCAACG	5045	0.0	39.10594	3
CAACGCA	4975	0.0	38.817524	5
GTACATG	6605	0.0	38.55718	1
TACATGG	6700	0.0	38.164394	2
AACGCAG	5150	0.0	37.473267	6
ACATGGG	7150	0.0	35.762444	3
ATGGGGG	3680	0.0	35.4709	5
GTGGTAT	760	0.0	35.39708	1
TGGTATC	780	0.0	35.15112	2
CATGGGG	6045	0.0	34.510216	4
TGTAAGT	560	0.0	30.958397	1
ACGCAGA	6280	0.0	30.62532	7
TGGGGGG	2990	0.0	30.280024	6
CGCAGAG	6465	0.0	29.656857	8
TAAGTAC	820	0.0	29.075155	3
>>END_MODULE