FastQCFastQC Report
Mon 15 Aug 2016
SRR2921306_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921306_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263258
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.056.7289541
GTATCAA26600.049.8865241
TCAACGC32150.040.5327384
GTACATG76000.039.4618071
CAACGCA33100.039.3662955
TACATGG78600.037.8521272
AACGCAG34750.037.6668366
ACATGGG80050.036.5718233
TATCAAC35950.036.4138532
ATCAACG35650.036.3864563
CATGGGG69100.035.306014
ATGGGGG46250.033.3193475
TGGGGGG33750.032.612886
ACGCAGA43750.029.9146757
CGCAGAG45450.028.7957558
GTATAGG4500.027.7694191
CCCTATA2256.546543E-926.446022
GCAGAGT51400.024.7679659
GTAAGTA11300.023.169522
ATATAGG4700.022.788592
Produced by FastQC (version 0.11.5)