FastQCFastQC Report
Mon 15 Aug 2016
SRR2921306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263258
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34950.27666557425324045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27340.21642451502385104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.21191237261113724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.072.089231
GTATCAA43050.051.210151
GTACATG75050.036.6989061
TCAACGC58050.036.439624
TATCAAC60500.036.0473632
TACATGG77550.035.0373152
CAACGCA59900.035.0157625
ATCAACG60950.034.6080673
ACATGGG79200.034.3826073
AACGCAG62200.033.7182926
GTGGTAT11100.033.371181
CATGGGG68650.032.4622654
ATGGGGG44750.032.0901535
TGGTATC11900.030.0436062
ACGCAGA73650.028.1482227
CGCAGAG76100.027.161578
TGGGGGG35400.026.7613016
CTATAGC3950.025.6448153
TAAGTAC11050.024.3306161
GAGTACT42300.024.20502912-13
Produced by FastQC (version 0.11.5)