##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1263258
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.026825874049482	31.0	30.0	34.0	16.0	34.0
2	29.81485096472771	33.0	31.0	34.0	25.0	34.0
3	30.04692469788436	33.0	31.0	34.0	26.0	34.0
4	32.16375118938491	37.0	33.0	37.0	19.0	37.0
5	31.797925681056444	37.0	33.0	37.0	19.0	37.0
6	31.94941492553382	37.0	33.0	37.0	17.0	37.0
7	32.16952119044566	37.0	35.0	37.0	17.0	37.0
8	32.435420159619014	37.0	35.0	37.0	17.0	37.0
9	34.16075496850208	39.0	37.0	39.0	17.0	39.0
10-11	34.37795010995379	39.0	37.0	39.0	18.0	39.0
12-13	34.38329699871285	39.0	37.0	39.0	20.0	39.0
14-15	35.74543838234153	40.0	37.5	41.0	22.5	41.0
16-17	35.77075031387096	40.0	38.0	41.0	23.0	41.0
18-19	35.829137832493444	40.5	38.0	41.0	23.5	41.0
20-21	35.8364304837175	41.0	38.0	41.0	23.5	41.0
22-23	35.78302650764927	40.5	38.0	41.0	23.0	41.0
24-25	35.73169138845746	41.0	37.5	41.0	23.0	41.0
26-27	35.621399983218	40.0	37.0	41.0	21.5	41.0
28-29	35.539797491882105	40.0	37.0	41.0	20.0	41.0
30-31	35.419095703332175	40.0	36.5	41.0	18.5	41.0
32-33	35.25708723000369	40.0	36.0	41.0	17.5	41.0
34-35	35.166663500250934	40.0	36.0	41.0	16.0	41.0
36-37	35.08202204141988	40.0	35.0	41.0	15.5	41.0
38-39	34.98243984997522	40.0	35.0	41.0	14.0	41.0
40-41	34.84199268874609	40.0	35.0	41.0	9.0	41.0
42-43	34.68036814332464	40.0	35.0	41.0	7.5	41.0
44-45	34.49456999282807	40.0	35.0	41.0	2.0	41.0
46-47	34.201017923496224	40.0	35.0	41.0	2.0	41.0
48-49	33.927928815808016	40.0	35.0	41.0	2.0	41.0
50-51	33.73044500806645	40.0	35.0	41.0	2.0	41.0
52-53	33.55434835955917	39.0	35.0	41.0	2.0	41.0
54-55	33.353698927693316	39.0	34.0	41.0	2.0	41.0
56-57	33.12343203051158	39.0	34.0	41.0	2.0	41.0
58-59	33.06317276439175	38.5	34.0	41.0	2.0	41.0
60-61	32.82619662808389	38.0	34.0	41.0	2.0	41.0
62-63	32.5404592727693	37.0	34.0	40.0	2.0	41.0
64-65	32.25032772402787	37.0	34.0	40.0	2.0	41.0
66-67	31.934879889935388	36.0	33.0	39.0	2.0	41.0
68-69	31.596549952582926	36.0	33.0	39.0	2.0	41.0
70-71	31.265746981218406	35.0	33.0	38.5	2.0	40.5
72-73	30.9221560441335	35.0	33.0	37.0	2.0	39.5
74-75	30.585966603813315	35.0	32.5	37.0	2.0	39.0
76-77	30.2837706153454	35.0	32.0	36.5	2.0	39.0
78-79	30.025193190939618	35.0	32.0	36.0	2.0	37.5
80-81	29.795687420938556	35.0	32.0	35.5	2.0	37.0
82-83	29.586384966491405	35.0	32.0	35.0	2.0	37.0
84-85	29.407657818117915	35.0	32.0	35.0	2.0	36.0
86-87	29.26557718217498	35.0	31.0	35.0	2.0	36.0
88-89	29.138243731684263	35.0	31.0	35.0	2.0	36.0
90-91	29.02862558558901	35.0	31.0	35.0	2.0	35.5
92-93	28.956810881071007	35.0	31.0	35.0	2.0	35.0
94-95	28.884550107737297	35.0	31.0	35.0	2.0	35.0
96-97	28.81416385251469	35.0	31.0	35.0	2.0	35.0
98-99	28.760150341418775	35.0	31.0	35.0	2.0	35.0
100-101	28.70715562458342	35.0	31.0	35.0	2.0	35.0
102-103	28.042429574956184	34.0	29.5	35.0	2.0	35.0
104-105	28.573042086414652	35.0	30.5	35.0	2.0	35.0
106-107	28.62516405991492	35.0	31.0	35.0	2.0	35.0
108-109	28.697280365531032	35.0	31.0	35.0	2.0	35.0
110-111	28.797738862528476	35.0	31.5	35.0	2.0	35.0
112-113	28.757972243199728	35.0	31.0	35.0	2.0	35.0
114-115	28.697161229139255	35.0	31.0	35.0	2.0	35.0
116-117	28.636659732216224	35.0	31.0	35.0	2.0	35.0
118-119	28.566011456092106	35.0	31.0	35.0	2.0	35.0
120-121	28.469660196096125	35.0	31.0	35.0	2.0	35.0
122-123	28.359528299048968	35.0	31.0	35.0	2.0	35.0
124-125	27.844618043186742	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90005.0
3	5339.0
4	4175.0
5	3891.0
6	4242.0
7	4440.0
8	4584.0
9	4654.0
10	4770.0
11	5032.0
12	5958.0
13	7877.0
14	10515.0
15	9303.0
16	5242.0
17	4548.0
18	3964.0
19	3430.0
20	3183.0
21	3128.0
22	3479.0
23	4170.0
24	5644.0
25	7460.0
26	8465.0
27	9342.0
28	11274.0
29	13284.0
30	15757.0
31	19605.0
32	25765.0
33	34150.0
34	52851.0
35	90111.0
36	191325.0
37	427299.0
38	154748.0
39	249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8222280823267	18.646861278618314	12.877050065767317	25.653860573287666
2	15.921609045816451	20.000031664157284	38.54145392310993	25.53690536691634
3	18.961605626087465	23.928920299732912	29.133795313388084	27.97567876079154
4	13.218813983648689	16.446558956404424	32.19194025738351	38.14268680256339
5	13.699503560722611	38.35429586512078	31.927865628320827	16.01833494583578
6	33.000542507652526	34.734903833588746	16.66599348198835	15.598560176770377
7	29.017770894498973	32.713679883195404	18.618899667765668	19.649649554539955
8	27.866990089886112	35.279308777868735	18.00595968504236	18.847741447202797
9	27.83594685142846	16.43729773206584	18.11346954026992	37.61328587623577
10-11	26.70458278678337	26.021847221441597	25.255793297641677	22.01777669413336
12-13	26.198378756634888	23.294967840864853	26.409261577466864	24.097391825033394
14-15	23.8496807461342	24.271051519167106	24.3500535915862	27.52921414311249
16-17	23.419523169455488	27.31666848735571	24.979220396783557	24.28458794640525
18-19	23.595615464141133	26.103416720891538	27.329571631448207	22.971396183519122
20-21	24.38266767358687	25.052958303054485	27.394403993483518	23.169970029875135
22-23	24.18741856374549	24.346808015464774	27.31449157654256	24.151281844247176
24-25	23.934580267847107	24.82545133298186	27.09719629719345	24.142772101977584
26-27	23.38247610543531	25.02976430784527	27.482984473480478	24.104775113238944
28-29	23.42775585034886	25.68343125473973	27.126881444645512	23.761931450265898
30-31	24.51142205313562	25.041915428202316	27.111524328363647	23.33513819029842
32-33	24.029018616941276	25.17557775212981	27.05710155803486	23.738302072894058
34-35	23.99359434098181	25.45810119548026	27.389179407531955	23.159125056005976
36-37	24.041921761033773	25.306271561312098	27.444314621399585	23.20749205625454
38-39	23.906359587669343	25.562474173921718	27.173467336046954	23.357698902361985
40-41	23.85296590245223	25.47140014153878	27.171884128182842	23.503749827826145
42-43	23.5821977774928	25.619390496636473	26.89407072822812	23.904340997642603
44-45	23.429259897819765	25.600708643839976	27.308000424299706	23.662031034040552
46-47	23.534978602945717	25.570746435011692	26.591282224217068	24.302992737825527
48-49	23.55765805559909	26.0712380210535	26.25374230758879	24.117361615758618
50-51	23.640499407088654	26.083468301803748	26.378894889246695	23.897137401860903
52-53	23.779821699130345	26.054970560249767	26.20054652335469	23.9646612172652
54-55	23.304146896358464	25.57502109624479	26.787006296417676	24.33382571097907
56-57	23.49318191533321	25.802686387103822	26.17739210834208	24.526739589220888
58-59	23.648296705819398	25.89193973044303	26.513902939858685	23.94586062387889
60-61	24.306277894143555	25.659643556581475	26.63220814750431	23.401870401770662
62-63	23.841804287010255	25.682520910217864	26.66355566321369	23.81211913955819
64-65	23.462348942179663	25.370074838235734	26.707648002229156	24.459928217355444
66-67	23.553066752793175	25.78978324301133	26.414833707761993	24.242316296433508
68-69	23.1982698704461	25.74470139908079	26.623737985431323	24.433290745041788
70-71	23.413863201341297	25.60850594257072	26.756687865819966	24.22094299026802
72-73	23.48787816898844	26.121386130149187	26.185743529825263	24.204992171037112
74-75	23.381565760913446	26.137020307807273	26.327559374252925	24.153854557026357
76-77	23.298724409423887	25.419114701826544	26.44008587319455	24.842075015555018
78-79	23.166130750804665	25.57003399147284	26.682197935813583	24.581637321908907
80-81	23.71479143611202	25.875038986493653	25.999122902843286	24.41104667455104
82-83	23.537670056314706	25.949766397679653	26.33282354040109	24.179740005604554
84-85	23.515703047200176	26.072346266558377	26.04305692107234	24.368893765169112
86-87	22.969971296441425	26.452395314338006	26.173513249075008	24.40412014014556
88-89	22.922158418945298	25.973870737410724	26.321266122993087	24.782704720650887
90-91	23.382885296927853	25.508152365361493	26.460574405918685	24.64838793179197
92-93	23.49044132198694	25.718226795962796	26.26309123293093	24.528240649119333
94-95	23.50481612594755	25.80593323918509	25.879829583235704	24.809421051631656
96-97	23.04123370622058	25.759221943707917	26.14778304065583	25.05176130941567
98-99	22.732894916515438	25.67315056510648	26.11431161105317	25.479642907324912
100-101	22.924533230741464	25.874959826100447	26.214993295114695	24.98551364804339
102-103	22.831480188528392	25.85572385055151	26.154316853722676	25.158479107197422
104-105	23.151732722742594	25.908019544708814	25.909167370864612	25.031080361683976
106-107	23.127406235243967	25.607587386389678	26.14983758221324	25.11516879615311
108-109	23.068602045755807	25.5493867205172	26.123263852469364	25.258747381257624
110-111	22.807590418527443	26.18300962477355	26.00557054994239	25.003829406756616
112-113	22.767180739143445	26.242552915267492	26.038752547009846	24.951513798579217
114-115	22.914360283759805	26.283008569552464	25.829812986566807	24.972818160120926
116-117	22.621831922748253	25.991997603001472	25.96927836332814	25.416892110922134
118-119	22.597263809095963	25.64008383122139	26.315522826988268	25.447129532694373
120-121	22.6193392875166	25.713918183743218	26.502197691286216	25.16454483745396
122-123	22.522917260378076	26.02818941768785	26.12698299610525	25.321910325828824
124-125	22.234421723203702	25.909642333411835	25.896818344636785	25.95911759874768
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	69.5
2	88.5
3	101.5
4	86.5
5	114.0
6	251.0
7	781.0
8	1183.0
9	1324.0
10	1263.5
11	980.0
12	978.0
13	1063.5
14	1155.5
15	1263.0
16	1381.0
17	1483.5
18	1541.0
19	1564.5
20	1665.0
21	1766.5
22	1879.5
23	2066.0
24	2317.0
25	2662.0
26	3267.0
27	4001.0
28	4578.5
29	5477.0
30	6824.5
31	8398.5
32	10435.0
33	12690.0
34	14898.5
35	17139.0
36	19784.0
37	22483.5
38	24429.0
39	26505.5
40	28940.0
41	30726.0
42	32829.0
43	36357.0
44	39305.5
45	42098.0
46	45095.0
47	47875.5
48	51834.5
49	55656.5
50	58364.5
51	59524.0
52	58537.5
53	56711.0
54	55254.5
55	53090.5
56	49636.5
57	45020.0
58	40268.5
59	35210.0
60	29252.5
61	23903.0
62	19307.0
63	14943.0
64	10861.0
65	7876.0
66	5949.0
67	4436.0
68	3254.5
69	2455.0
70	1898.5
71	1430.5
72	1069.5
73	817.0
74	623.5
75	453.0
76	330.5
77	269.5
78	226.0
79	164.0
80	138.0
81	123.0
82	114.5
83	114.0
84	103.0
85	98.5
86	90.5
87	65.5
88	57.5
89	66.5
90	64.0
91	63.5
92	62.0
93	52.0
94	56.0
95	54.5
96	40.0
97	34.0
98	24.0
99	7.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3383315205603289
2	0.0
3	0.0
4	0.01899849436932123
5	0.1803273757221407
6	0.04773371710291959
7	0.023273155602418508
8	0.008232680893372535
9	0.00649115224285142
10-11	0.002453972189370659
12-13	0.05608513858610038
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.9580196602752565E-4
92-93	6.33283145644041E-4
94-95	1.5832078641101025E-4
96-97	5.937029490412885E-4
98-99	3.958019660275256E-5
100-101	0.0
102-103	0.0
104-105	3.958019660275256E-5
106-107	2.77061376219268E-4
108-109	6.728633422467936E-4
110-111	5.937029490412885E-4
112-113	9.499247184660615E-4
114-115	5.145425558357834E-4
116-117	9.10344521863309E-4
118-119	4.353821626302782E-4
120-121	3.958019660275256E-5
122-123	0.0014248870776990923
124-125	3.958019660275256E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1263258.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.36504911292089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.38728609871357	53.672267651368834
2	9.163171118909972	11.79575918197477
3	2.916052167228167	5.63075522878441
4	1.3283707765358046	3.4200260108758367
5	0.8194873939442947	2.637317317932203
6	0.4886594651144472	1.8871554282951022
7	0.34380066254331326	1.5490122570758569
8	0.259668803434983	1.3370876228948863
9	0.1887036925723108	1.0931330196185676
>10	1.0365450988146319	11.889058667410774
>50	0.04956303886371643	2.1528991073288877
>100	0.017830889427716646	1.9208247471455702
>500	1.2297055672010578E-4	0.04408658298962187
>1k	7.378233403206347E-4	0.9706171763047271
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3495	0.27666557425324045	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2734	0.21642451502385104	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2677	0.21191237261113724	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.916039320550512E-5
7	0.0	0.0	0.0	0.0	7.916039320550512E-5
8	0.0	0.0	0.0	0.0	7.916039320550512E-5
9	0.0	0.0	0.0	0.0	1.5832078641101025E-4
10-11	0.0	0.0	0.0	0.0	1.5832078641101025E-4
12-13	7.916039320550512E-5	0.0	0.0	0.0	2.3748117961651537E-4
14-15	7.916039320550512E-5	0.0	0.0	0.0	3.166415728220205E-4
16-17	7.916039320550512E-5	0.0	0.0	0.0	3.166415728220205E-4
18-19	7.916039320550512E-5	0.0	0.0	0.0	3.166415728220205E-4
20-21	1.5832078641101025E-4	0.0	0.0	0.0	3.9580196602752565E-4
22-23	1.5832078641101025E-4	0.0	0.0	1.9790098301376282E-4	5.145425558357833E-4
24-25	1.5832078641101025E-4	0.0	0.0	5.937029490412885E-4	6.33283145644041E-4
26-27	1.5832078641101025E-4	0.0	0.0	8.311841286578038E-4	6.33283145644041E-4
28-29	1.5832078641101025E-4	0.0	0.0	0.002216491009754144	6.33283145644041E-4
30-31	1.5832078641101025E-4	0.0	0.0	0.007757718534139503	6.33283145644041E-4
32-33	1.5832078641101025E-4	0.0	0.0	0.01959219731836252	6.33283145644041E-4
34-35	1.9790098301376282E-4	0.0	0.0	0.03597839871190209	6.33283145644041E-4
36-37	2.374811796165154E-4	0.0	0.0	0.05913281372451233	7.124435388495461E-4
38-39	2.374811796165154E-4	0.0	0.0	0.1019585864486906	7.124435388495461E-4
40-41	3.166415728220205E-4	0.0	0.0	0.1572917012993387	7.124435388495461E-4
42-43	3.166415728220205E-4	0.0	0.0	0.20364011152116196	7.124435388495461E-4
44-45	3.166415728220205E-4	0.0	0.0	0.25814204224315224	7.520237354522988E-4
46-47	3.166415728220205E-4	0.0	0.0	0.3115753076568682	7.916039320550513E-4
48-49	3.166415728220205E-4	0.0	0.0	0.3736370559299842	7.916039320550513E-4
50-51	3.166415728220205E-4	0.0	0.0	0.4440898058828838	7.916039320550513E-4
52-53	3.9580196602752565E-4	0.0	0.0	0.5188963774620861	9.895049150688141E-4
54-55	3.9580196602752565E-4	0.0	0.0	0.6045875031070453	0.0013853068810963398
56-57	3.9580196602752565E-4	0.0	0.0	0.6894870248199496	0.0015040474709045975
58-59	3.9580196602752565E-4	0.0	0.0	0.7857460629578439	0.0015040474709045975
60-61	3.9580196602752565E-4	0.0	0.0	0.8824009030617657	0.0015040474709045975
62-63	3.9580196602752565E-4	0.0	0.0	0.9926317506004316	0.0015832078641101026
64-65	3.9580196602752565E-4	0.0	0.0	1.0991024794618358	0.0015832078641101026
66-67	3.9580196602752565E-4	0.0	0.0	1.2198616592968339	0.0015832078641101026
68-69	3.9580196602752565E-4	0.0	0.0	1.3574820028846046	0.0015832078641101026
70-71	3.9580196602752565E-4	0.0	0.0	1.5055910985721048	0.0015832078641101026
72-73	4.353821626302782E-4	0.0	0.0	1.6430531213734645	0.0017811088471238654
74-75	4.749623592330308E-4	0.0	0.0	1.7836419797064416	0.001820689043726618
76-77	4.749623592330308E-4	0.0	0.0	1.9425564690664932	0.0018602692403293705
78-79	4.749623592330308E-4	0.0	0.0	2.129454157424691	0.0019790098301376282
80-81	4.749623592330308E-4	7.916039320550512E-5	0.0	2.315758142833847	0.0020185900267403805
82-83	5.145425558357834E-4	7.916039320550512E-5	0.0	2.507524195374183	0.0021373306165486383
84-85	5.54122752438536E-4	7.916039320550512E-5	0.0	2.7091852970652077	0.0021373306165486383
86-87	5.54122752438536E-4	7.916039320550512E-5	0.0	2.9246598873705922	0.002176910813151391
88-89	5.54122752438536E-4	7.916039320550512E-5	0.0	3.1723131775140154	0.002216491009754144
90-91	5.54122752438536E-4	7.916039320550512E-5	0.0	3.430257318774154	0.002216491009754144
92-93	5.54122752438536E-4	7.916039320550512E-5	0.0	3.68630161059736	0.002216491009754144
94-95	5.54122752438536E-4	7.916039320550512E-5	0.0	3.94642266267065	0.002295651402959649
96-97	5.54122752438536E-4	7.916039320550512E-5	0.0	4.240266042249485	0.0023352315995624016
98-99	5.54122752438536E-4	7.916039320550512E-5	0.0	4.585801158591515	0.002374811796165154
100-101	5.54122752438536E-4	7.916039320550512E-5	0.0	4.949147363404784	0.002374811796165154
102-103	5.54122752438536E-4	7.916039320550512E-5	0.0	5.327375722140687	0.0025727127791789167
104-105	5.54122752438536E-4	7.916039320550512E-5	0.0	5.723454749544432	0.0026122929757816694
106-107	5.54122752438536E-4	7.916039320550512E-5	0.0	6.15840153001208	0.0026914533689871745
108-109	5.54122752438536E-4	7.916039320550512E-5	0.0	6.60344126061343	0.0026914533689871745
110-111	5.54122752438536E-4	7.916039320550512E-5	0.0	7.076305869426514	0.0027706137621926795
112-113	5.54122752438536E-4	7.916039320550512E-5	0.0	7.547270628802668	0.0027706137621926795
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2420	0.0	72.08923	1
GTATCAA	4305	0.0	51.21015	1
GTACATG	7505	0.0	36.698906	1
TCAACGC	5805	0.0	36.43962	4
TATCAAC	6050	0.0	36.047363	2
TACATGG	7755	0.0	35.037315	2
CAACGCA	5990	0.0	35.015762	5
ATCAACG	6095	0.0	34.608067	3
ACATGGG	7920	0.0	34.382607	3
AACGCAG	6220	0.0	33.718292	6
GTGGTAT	1110	0.0	33.37118	1
CATGGGG	6865	0.0	32.462265	4
ATGGGGG	4475	0.0	32.090153	5
TGGTATC	1190	0.0	30.043606	2
ACGCAGA	7365	0.0	28.148222	7
CGCAGAG	7610	0.0	27.16157	8
TGGGGGG	3540	0.0	26.761301	6
CTATAGC	395	0.0	25.644815	3
TAAGTAC	1105	0.0	24.330616	1
GAGTACT	4230	0.0	24.205029	12-13
>>END_MODULE