FastQCFastQC Report
Mon 15 Aug 2016
SRR2921305_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921305_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600419
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.064.8325651
GTATCAA30050.051.892941
GTACATG88850.039.25471
TCAACGC39550.038.822544
CAACGCA40800.037.1943665
TACATGG94700.036.3864172
TATCAAC42800.036.0136152
AACGCAG42400.035.5067636
ACATGGG96600.035.0546723
ATCAACG45050.033.9507263
CATGGGG86600.033.467394
ATGGGGG57000.031.4259785
TGGGGGG42100.029.9647186
ACGCAGA53450.027.825327
CGCAGAG56100.026.5109338
GTATAGG4050.026.4526351
GTGGTAT9650.024.054081
ATCGCGT1250.001635320723.7962118
GCAGAGT61150.023.5425349
AGAGTAC55050.023.07108910-11
Produced by FastQC (version 0.11.5)