##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921305_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1600419
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.29477218153496	31.0	31.0	34.0	26.0	34.0
2	30.418987777575747	33.0	31.0	34.0	26.0	34.0
3	30.601125080369577	33.0	31.0	34.0	26.0	34.0
4	33.98626172271136	37.0	35.0	37.0	30.0	37.0
5	34.07207175121015	37.0	35.0	37.0	30.0	37.0
6	34.08420419902538	37.0	35.0	37.0	30.0	37.0
7	34.03788132982675	37.0	35.0	37.0	30.0	37.0
8	33.94626782111435	37.0	35.0	37.0	30.0	37.0
9	35.56453528732163	39.0	37.0	39.0	30.0	39.0
10-11	35.57249226608782	39.0	37.0	39.0	30.0	39.0
12-13	35.491129510459444	39.0	37.0	39.0	30.0	39.0
14-15	36.80853576469662	41.0	38.0	41.0	30.0	41.0
16-17	36.793757759686684	41.0	38.0	41.0	30.0	41.0
18-19	36.80558966120747	41.0	38.0	41.0	30.0	41.0
20-21	36.84465443112085	41.0	38.0	41.0	30.0	41.0
22-23	36.81451076249407	41.0	38.0	41.0	30.0	41.0
24-25	36.815239634120815	41.0	38.0	41.0	30.0	41.0
26-27	36.78778401156197	41.0	38.0	41.0	30.0	41.0
28-29	36.7216219627485	40.0	38.0	41.0	30.0	41.0
30-31	36.64350929350377	40.0	38.0	41.0	30.0	41.0
32-33	36.55189640962773	40.0	37.5	41.0	30.0	41.0
34-35	36.4488886972724	40.0	37.0	41.0	29.0	41.0
36-37	36.34564979545981	40.0	37.0	41.0	28.0	41.0
38-39	36.24969898507828	40.0	37.0	41.0	28.0	41.0
40-41	36.14366175357828	40.0	37.0	41.0	27.5	41.0
42-43	35.96834860121006	40.0	36.5	41.0	27.0	41.0
44-45	35.757223577075756	40.0	36.0	41.0	26.5	41.0
46-47	35.544463668576796	40.0	35.0	41.0	26.0	41.0
48-49	35.343469116525114	40.0	35.0	41.0	25.5	41.0
50-51	35.172479831844036	39.5	35.0	41.0	25.0	41.0
52-53	34.99239011783789	39.0	35.0	41.0	24.0	41.0
54-55	34.79027804593672	39.0	35.0	41.0	24.0	41.0
56-57	34.547990869890945	39.0	35.0	40.5	23.0	41.0
58-59	34.28232856520699	38.0	34.0	40.0	23.0	41.0
60-61	33.99787430666594	38.0	34.0	40.0	22.0	41.0
62-63	33.667920713263214	37.0	34.0	40.0	21.5	41.0
64-65	33.31942135153356	36.5	34.0	39.5	20.5	41.0
66-67	32.97219884292801	36.0	33.0	39.0	19.5	41.0
68-69	32.60651616857835	35.5	33.0	39.0	18.5	40.0
70-71	32.53950371746399	35.0	33.0	38.5	18.5	40.0
72-73	32.241998501642385	35.0	33.0	37.5	18.0	39.5
74-75	31.913079949688175	35.0	33.0	37.0	16.5	39.0
76-77	31.610471070388442	35.0	33.0	36.5	13.5	39.0
78-79	31.329787386928047	35.0	33.0	36.0	11.5	38.0
80-81	31.06639729970714	35.0	33.0	36.0	8.0	37.0
82-83	30.851692588003516	35.0	32.5	35.0	7.0	37.0
84-85	30.642464567091494	35.0	32.0	35.0	6.5	36.0
86-87	30.48193004456958	35.0	32.0	35.0	2.0	36.0
88-89	30.353345905041117	35.0	32.0	35.0	2.0	36.0
90-91	30.235960082953277	35.0	32.0	35.0	2.0	35.5
92-93	30.130143418692228	35.0	32.0	35.0	2.0	35.0
94-95	30.02139564701494	35.0	32.0	35.0	2.0	35.0
96-97	29.93296880379451	35.0	32.0	35.0	2.0	35.0
98-99	29.857245508832374	35.0	31.5	35.0	2.0	35.0
100-101	29.76956784442074	35.0	31.0	35.0	2.0	35.0
102-103	28.974886264159572	33.5	29.5	35.0	2.0	35.0
104-105	29.558105096227926	34.5	31.0	35.0	2.0	35.0
106-107	29.630777315190585	35.0	31.0	35.0	2.0	35.0
108-109	29.573492316699564	35.0	31.0	35.0	2.0	35.0
110-111	29.505134280460304	35.0	31.0	35.0	2.0	35.0
112-113	29.421653954370697	35.0	31.0	35.0	2.0	35.0
114-115	29.305574352716384	35.0	31.0	35.0	2.0	35.0
116-117	29.17584457570174	34.0	31.0	35.0	2.0	35.0
118-119	29.176630307438238	34.5	31.0	35.0	2.0	35.0
120-121	29.34327260548644	35.0	31.5	35.0	2.0	35.0
122-123	29.223477726770305	35.0	32.0	35.0	2.0	35.0
124-125	28.687737398768697	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59759.0
3	8739.0
4	5275.0
5	4245.0
6	4664.0
7	5180.0
8	5258.0
9	5491.0
10	6253.0
11	7056.0
12	7455.0
13	7656.0
14	7743.0
15	6652.0
16	4972.0
17	4644.0
18	4306.0
19	4002.0
20	3970.0
21	4247.0
22	4753.0
23	6488.0
24	10015.0
25	13053.0
26	12565.0
27	13223.0
28	16118.0
29	19290.0
30	23267.0
31	29158.0
32	37390.0
33	51334.0
34	81409.0
35	135238.0
36	290440.0
37	533564.0
38	155450.0
39	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89238534649926	18.13227295991541	13.038943476122538	24.93639821746279
2	16.290319456135318	19.073132902275955	39.8800563161654	24.756491325423326
3	20.019122012185598	23.774535228870487	28.243649429776596	27.962693329167315
4	13.58854054702615	14.868180295660085	34.666570013048236	36.876709144265526
5	14.38506222614373	37.983630487436045	32.66572600500409	14.96558128141614
6	34.08280783863703	33.76485463969783	16.98927634551956	15.163061176145574
7	29.7536878087495	32.34804290700254	19.472255117313093	18.426014166934863
8	27.55412268537758	35.692632265700624	18.781882593939407	17.971362454982387
9	28.037521004176153	16.4437156817226	18.120987803986434	37.39777551011481
10-11	26.768643761708788	25.771483239954655	26.075708588546853	21.3841644097897
12-13	26.029789769448442	23.03269138659932	26.70984359636809	24.227675247584145
14-15	23.904321725353313	24.410907433287623	23.882607338587903	27.802163502771158
16-17	24.261909612046395	27.461873869775662	24.496390155628845	23.779826362549098
18-19	24.260939415137734	26.862347691141586	25.939847862200534	22.936865031520153
20-21	25.042616103888314	25.69719820987369	25.745094461531153	23.51509122470684
22-23	24.9722167112147	25.05004428767147	25.801075713953193	24.176663287160636
24-25	24.46953926079644	25.13196492849446	25.787891938571438	24.610603872137663
26-27	24.136172797308586	25.533336790912482	25.839488761513895	24.491001650265034
28-29	24.14204980735175	26.30707743297303	25.565665995553406	23.98520676412181
30-31	25.12406785083614	25.595688150098667	25.596375506612993	23.683868492452202
32-33	24.733955468238374	25.457071691737028	25.604669386163557	24.204303453861037
34-35	24.528181036508244	25.87571299309628	25.772700800351934	23.82340517004354
36-37	24.479521741765797	25.938085629819778	25.753652267971084	23.82874036044334
38-39	24.6422794633403	26.005362097823138	25.553323691430364	23.7990347474062
40-41	24.644192603405354	25.542803117407693	25.778695797798417	24.034308481388535
42-43	24.252299247768864	25.738327544857047	25.511495926404077	24.497877280970016
44-45	23.951451845519735	25.845743444386954	25.972812733548796	24.229991976544515
46-47	23.959643009795858	25.641767503943093	25.714817768023856	24.683771718237196
48-49	23.966483553908073	25.96740338776308	25.667238854981818	24.39887420334702
50-51	24.110010016702837	25.90314354503798	25.856746777377378	24.130099660881807
52-53	24.15988007525915	25.879267929192412	25.682371900418477	24.278480095129964
54-55	23.844193904666092	25.43937237650536	26.141793294789018	24.57464042403953
56-57	24.17399210461772	25.71254497196624	25.563666869232538	24.549796054183506
58-59	24.077511498592923	26.05969676395961	25.86542123702083	23.997370500426637
60-61	24.65181541507052	25.873964631813372	25.825787264116016	23.648432689000096
62-63	24.447317461200214	25.890019002318894	25.749829254073063	23.91283428240783
64-65	24.259308669345643	25.51933530080363	25.738191897713207	24.48316413213752
66-67	24.226993031244582	25.776115649159642	25.63267894829749	24.364212371298287
68-69	23.926752035143025	25.76404369757876	25.874271824184884	24.434932443093324
70-71	23.952319001414697	25.728780812125397	26.031278275170838	24.287621911289072
72-73	23.996222085863504	26.293245119517305	25.454274491768697	24.256258302850494
74-75	23.984318469538614	26.197485056431262	25.609146476342108	24.209049997688012
76-77	23.870440638673788	25.603033091250495	25.730974054072632	24.795552216003085
78-79	23.751507463898974	25.76513556240119	25.991614439160944	24.491742534538886
80-81	24.132052004240272	26.079789698566486	25.46546281968008	24.32269547751316
82-83	24.26392824259148	26.02283957155261	25.734087799659633	23.97914438619628
84-85	24.284284615810027	26.171716701468096	25.32189109396886	24.22210758875302
86-87	23.7432196709449	26.51562088458514	25.54024705845963	24.20091238601033
88-89	23.684633955147756	25.97386123666575	25.758969871155784	24.582534937030704
90-91	24.258241011488536	25.660510349555043	25.783890805328515	24.29735783362791
92-93	24.259734578942847	25.975832467603478	25.5680307444756	24.196402208978075
94-95	24.201097852878718	25.930705105993823	25.459107651632767	24.409089389494692
96-97	23.767800143475966	25.92968802725543	25.62745740088535	24.675054428383245
98-99	23.65656371880797	25.94112322717776	25.572225605250487	24.830087448763784
100-101	23.641592255372252	26.083909507284176	25.734377689953064	24.540120547390508
102-103	23.529420953984193	26.077707073632606	25.734121795466624	24.65875017691658
104-105	23.86142341405043	26.09650746637048	25.55287383794407	24.489195281635027
106-107	23.894912400964067	25.943118271977244	25.749404956436372	24.412564370622313
108-109	23.8566739298473	25.916494876926894	25.703004857186958	24.523826336038855
110-111	23.55137827540763	26.482646658713975	25.635428923506076	24.330546142372324
112-113	23.56815717145372	26.532493220978147	25.62115648441286	24.278193123155276
114-115	23.755565067155715	26.641183215288823	25.445189751216112	24.15806196633935
116-117	23.57746310778466	26.430206986315362	25.552223343142515	24.44010656275746
118-119	23.581083766162042	26.201899533674588	25.707381196224965	24.509635503938405
120-121	23.487212238656554	26.26641862069526	25.956715269762494	24.289653870885697
122-123	23.51452753904279	26.472991139980433	25.656222243119885	24.356259077856887
124-125	23.42171044594914	26.39160132604722	25.564493276818133	24.622194951185502
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	399.0
1	356.0
2	287.0
3	269.5
4	226.5
5	186.5
6	186.5
7	231.0
8	313.5
9	365.5
10	386.5
11	422.5
12	486.0
13	539.5
14	570.0
15	628.0
16	712.0
17	754.5
18	814.0
19	895.5
20	970.0
21	1036.5
22	1181.5
23	1428.5
24	1744.0
25	2197.0
26	2754.0
27	3543.0
28	4488.0
29	5794.5
30	7539.5
31	9403.0
32	12014.5
33	14725.0
34	17347.5
35	20144.0
36	23623.0
37	27145.0
38	29793.5
39	33336.0
40	37057.0
41	39795.5
42	42753.5
43	46814.5
44	51231.5
45	55344.0
46	59350.0
47	63787.0
48	68676.0
49	72786.0
50	75050.0
51	76574.5
52	75539.0
53	73040.5
54	71610.5
55	68771.5
56	64161.0
57	58424.5
58	52528.5
59	46151.0
60	39049.5
61	32554.0
62	26525.5
63	20149.0
64	14311.0
65	10268.0
66	7726.5
67	5787.5
68	4204.0
69	3051.0
70	2322.0
71	1747.5
72	1247.5
73	921.5
74	641.5
75	429.5
76	301.0
77	187.0
78	106.0
79	78.5
80	60.5
81	50.0
82	40.5
83	30.0
84	25.5
85	20.5
86	16.5
87	14.0
88	8.5
89	7.0
90	9.5
91	6.5
92	6.5
93	7.0
94	6.0
95	4.0
96	0.5
97	1.0
98	3.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014246269258238
2	0.010497251032385893
3	0.010559734669483428
4	0.012684178330799623
5	0.007935421911386955
6	0.03111685127457247
7	0.013371498338872507
8	0.013246531064677438
9	0.008872676467849982
10-11	0.007810454637191886
12-13	0.006529540076692417
14-15	0.006092154617009671
16-17	0.0045613055081200615
18-19	0.00584222006861953
20-21	0.0053111091532904815
22-23	0.0054048346089367845
24-25	0.005529801883131855
26-27	0.0043738545968274554
28-29	0.005810978250070762
30-31	0.0053111091532904815
32-33	0.007685487362996815
34-35	0.007685487362996815
36-37	0.00699816735492393
38-39	0.007029409173472697
40-41	0.007185618266216534
42-43	0.007060650992021465
44-45	0.0069356837178263945
46-47	0.009435029201727797
48-49	0.008122872822679561
50-51	0.0057484946129732276
52-53	0.005123658241997877
54-55	0.006029670979912136
56-57	0.0072793437218628375
58-59	0.007154376447667767
60-61	0.0051549000605466445
62-63	0.005498560064583088
64-65	0.003499083677461965
66-67	0.0038427436814984076
68-69	0.0063420891653998114
70-71	0.004686272782315131
72-73	0.004061436411339781
74-75	0.004061436411339781
76-77	0.0044675800524737585
78-79	0.003061698217779219
80-81	0.0030929400363279864
82-83	0.0051549000605466445
84-85	0.009341303746081496
86-87	0.006841958262180091
88-89	0.0047175146008638985
90-91	0.005279867334741714
92-93	0.008216598278325863
94-95	0.007091892810570231
96-97	0.009435029201727797
98-99	0.006904441899277626
100-101	0.008466532826716004
102-103	0.005529801883131855
104-105	0.004779998237961433
106-107	0.007248101903314069
108-109	0.00687320008072886
110-111	0.00762300372589928
112-113	0.004842481875058969
114-115	0.002124443661316193
116-117	0.0029679727621329164
118-119	0.15624033456238648
120-121	0.011122087403361245
122-123	0.001718300020182215
124-125	0.0014683654717920745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1600419.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.1644073068658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9404027967069	49.50671764239561
2	10.305209279293887	12.606240354824486
3	3.2959294772770558	6.047807190086373
4	1.591989364284226	3.89492343621115
5	0.9146442110366924	2.797183553235758
6	0.6008831437042249	2.2051596807213114
7	0.43381144674519856	1.8573674016172832
8	0.31513137194260066	1.5419858870934917
9	0.2378107755317212	1.3090999622925446
>10	1.2890753273543647	13.962813433729673
>50	0.05864482454888449	2.4175137548876804
>100	0.015956620068935674	1.5609968161802557
>500	3.0681690318374173E-4	0.13661847497452034
>1k	2.0454460212249447E-4	0.15557241174983155
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.248363709753508E-5	6.248363709753508E-5
2	0.0	0.0	0.0	6.248363709753508E-5	6.248363709753508E-5
3	0.0	0.0	0.0	6.248363709753508E-5	6.248363709753508E-5
4	0.0	0.0	0.0	6.248363709753508E-5	6.248363709753508E-5
5	0.0	0.0	0.0	6.248363709753508E-5	6.248363709753508E-5
6	0.0	0.0	0.0	6.248363709753508E-5	6.248363709753508E-5
7	0.0	0.0	0.0	6.248363709753508E-5	6.248363709753508E-5
8	0.0	0.0	0.0	6.248363709753508E-5	6.248363709753508E-5
9	0.0	0.0	0.0	1.2496727419507016E-4	6.248363709753508E-5
10-11	0.0	0.0	0.0	1.2496727419507016E-4	6.248363709753508E-5
12-13	0.0	0.0	0.0	1.2496727419507016E-4	1.8745091129260524E-4
14-15	0.0	0.0	0.0	1.2496727419507016E-4	2.1869272984137276E-4
16-17	0.0	0.0	0.0	1.2496727419507016E-4	3.436600040364429E-4
18-19	0.0	0.0	0.0	1.2496727419507016E-4	3.7490182258521047E-4
20-21	0.0	0.0	0.0	1.2496727419507016E-4	4.06143641133978E-4
22-23	0.0	0.0	0.0	1.8745091129260524E-4	4.998690967802806E-4
24-25	0.0	0.0	0.0	2.499345483901403E-4	5.623527338778157E-4
26-27	0.0	0.0	0.0	5.935945524265832E-4	5.935945524265832E-4
28-29	0.0	0.0	0.0	0.00203071820566989	6.248363709753508E-4
30-31	0.0	0.0	0.0	0.0072793437218628375	6.560781895241184E-4
32-33	0.0	0.0	0.0	0.02080705115347918	6.87320008072886E-4
34-35	0.0	0.0	0.0	0.038708613181922986	6.87320008072886E-4
36-37	0.0	0.0	0.0	0.06254612073463262	6.87320008072886E-4
38-39	0.0	0.0	0.0	0.10622218306580963	8.12287282267956E-4
40-41	0.0	0.0	0.0	0.163425952828603	8.12287282267956E-4
42-43	0.0	0.0	0.0	0.21247560795016807	8.435291008167237E-4
44-45	0.0	0.0	0.0	0.26349349764030544	8.747709193654912E-4
46-47	0.0	0.0	0.0	0.31838537283049	9.684963750117938E-4
48-49	0.0	0.0	0.0	0.38011920628285467	9.997381935605613E-4
50-51	0.0	0.0	0.0	0.45269395077164165	9.997381935605613E-4
52-53	0.0	0.0	0.0	0.5277055571072324	0.0010934636492068639
54-55	0.0	0.0	0.0	0.6108400362655029	0.0011871891048531667
56-57	0.0	0.0	0.0	0.6922249735850424	0.0011871891048531667
58-59	0.0	0.0	0.0	0.7900431074612335	0.0011871891048531667
60-61	0.0	0.0	0.0	0.8916415013818256	0.0011871891048531667
62-63	0.0	0.0	0.0	1.0121099537058733	0.0012496727419507017
64-65	0.0	0.0	0.0	1.1215812859007548	0.0012496727419507017
66-67	0.0	0.0	0.0	1.242237189136095	0.001280914560499469
68-69	0.0	0.0	0.0	1.3784202761901727	0.0013433981975970043
70-71	0.0	0.0	0.0	1.5232885888008076	0.001374640016145772
72-73	0.0	0.0	0.0	1.6601589958629583	0.0014371236532433069
74-75	0.0	0.0	0.0	1.8004347611469247	0.0015308491088896095
76-77	0.0	0.0	0.0	1.9669848958304046	0.001562090927438377
78-79	0.0	0.0	0.0	2.1581535835303134	0.001562090927438377
80-81	0.0	0.0	0.0	2.3369192692663607	0.0015933327459871447
82-83	0.0	0.0	0.0	2.523557893276698	0.0016870582016334473
84-85	0.0	0.0	0.0	2.7370644812389755	0.0016870582016334473
86-87	0.0	0.0	0.0	2.970597074891013	0.0017495418387309823
88-89	0.0	0.0	0.0	3.236121290736988	0.0018120254758285173
90-91	0.0	0.0	0.0	3.505738184812852	0.0018120254758285173
92-93	0.0	0.0	0.0	3.7504241076867997	0.0018745091129260525
94-95	0.0	0.0	0.0	4.003389112476171	0.0018745091129260525
96-97	0.0	0.0	0.0	4.30199841416529	0.00203071820566989
98-99	0.0	0.0	0.0	4.646595672758196	0.002249410935511263
100-101	0.0	0.0	0.0	5.000940378738318	0.002249410935511263
102-103	0.0	0.0	0.0	5.363407957541119	0.002249410935511263
104-105	0.0	0.0	0.0	5.741683896529596	0.002249410935511263
106-107	0.0	0.0	0.0	6.165729099692019	0.0022806527540600308
108-109	0.0	0.0	0.0	6.6107688049192115	0.0023743782097063334
110-111	0.0	0.0	0.0	7.09348614331622	0.0023743782097063334
112-113	0.0	0.0	0.0	7.555771332382332	0.0023743782097063334
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1680	0.0	64.832565	1
GTATCAA	3005	0.0	51.89294	1
GTACATG	8885	0.0	39.2547	1
TCAACGC	3955	0.0	38.82254	4
CAACGCA	4080	0.0	37.194366	5
TACATGG	9470	0.0	36.386417	2
TATCAAC	4280	0.0	36.013615	2
AACGCAG	4240	0.0	35.506763	6
ACATGGG	9660	0.0	35.054672	3
ATCAACG	4505	0.0	33.950726	3
CATGGGG	8660	0.0	33.46739	4
ATGGGGG	5700	0.0	31.425978	5
TGGGGGG	4210	0.0	29.964718	6
ACGCAGA	5345	0.0	27.82532	7
CGCAGAG	5610	0.0	26.510933	8
GTATAGG	405	0.0	26.452635	1
GTGGTAT	965	0.0	24.05408	1
ATCGCGT	125	0.0016353207	23.796211	8
GCAGAGT	6115	0.0	23.542534	9
AGAGTAC	5505	0.0	23.071089	10-11
>>END_MODULE