FastQCFastQC Report
Mon 15 Aug 2016
SRR2921305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600419
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.19632358776045522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.15358477998574124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23550.1471489653646951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.072.121111
GTATCAA41450.052.044521
GTACATG82700.041.0426331
TACATGG85950.039.2469562
ACATGGG88450.037.598613
TCAACGC56200.036.5862734
TATCAAC57800.036.501512
CAACGCA57850.035.336725
CATGGGG81550.034.714034
ATCAACG60050.034.5383533
AACGCAG60100.033.603466
ATGGGGG52450.033.1797265
GTGGTAT11350.033.1693571
TGGTATC11450.032.7921942
ACGCAGA72800.027.3261687
CGCAGAG74950.026.5373178
TGGGGGG41000.026.4451666
GTAAGTA7700.024.7682112
AGAGTAC77450.024.14068610-11
GCAGAGT84050.023.5209779
Produced by FastQC (version 0.11.5)