##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1600419
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.12762470328083	31.0	30.0	34.0	16.0	34.0
2	29.896034725906155	33.0	31.0	34.0	26.0	34.0
3	30.127461620988004	33.0	31.0	34.0	26.0	34.0
4	32.3007387440414	37.0	35.0	37.0	19.0	37.0
5	31.936893401040603	37.0	33.0	37.0	19.0	37.0
6	32.079270491040155	37.0	35.0	37.0	17.0	37.0
7	32.28419057759249	37.0	35.0	37.0	17.0	37.0
8	32.54319150172549	37.0	35.0	37.0	17.0	37.0
9	34.2719862735946	39.0	37.0	39.0	17.0	39.0
10-11	34.467295439506785	39.0	37.0	39.0	20.0	39.0
12-13	34.47072110491065	39.0	37.0	39.0	22.5	39.0
14-15	35.84638928930487	40.0	38.0	41.0	24.5	41.0
16-17	35.86572297629559	40.0	38.0	41.0	25.0	41.0
18-19	35.909409660845064	40.5	38.0	41.0	25.0	41.0
20-21	35.912384819225466	41.0	38.0	41.0	25.0	41.0
22-23	35.86352292743338	40.5	38.0	41.0	24.0	41.0
24-25	35.81602724036643	41.0	38.0	41.0	24.0	41.0
26-27	35.7142704504258	40.0	37.0	41.0	23.0	41.0
28-29	35.63437980928744	40.0	37.0	41.0	22.5	41.0
30-31	35.52474633205429	40.0	37.0	41.0	21.5	41.0
32-33	35.379298483709576	40.0	36.0	41.0	19.0	41.0
34-35	35.29493026513681	40.0	36.0	41.0	18.0	41.0
36-37	35.21866242527739	40.0	36.0	41.0	17.0	41.0
38-39	35.124132805221635	40.0	35.5	41.0	16.0	41.0
40-41	34.984219445032835	40.0	35.0	41.0	14.0	41.0
42-43	34.83467766878549	40.0	35.0	41.0	9.0	41.0
44-45	34.667259323964544	40.0	35.0	41.0	7.0	41.0
46-47	34.41709795997173	40.0	35.0	41.0	2.0	41.0
48-49	34.17633975852574	40.0	35.0	41.0	2.0	41.0
50-51	33.98258112406813	40.0	35.0	41.0	2.0	41.0
52-53	33.800321665763775	39.0	35.0	41.0	2.0	41.0
54-55	33.60383187152864	39.0	35.0	41.0	2.0	41.0
56-57	33.36609412909994	39.0	34.0	41.0	2.0	41.0
58-59	33.30053473496628	38.5	34.0	41.0	2.0	41.0
60-61	33.060469789473885	38.0	34.0	41.0	2.0	41.0
62-63	32.77612144069772	37.0	34.0	40.0	2.0	41.0
64-65	32.477995449941545	37.0	34.0	40.0	2.0	41.0
66-67	32.15825480702241	36.0	34.0	39.0	2.0	41.0
68-69	31.82452813919355	36.0	33.0	39.0	2.0	41.0
70-71	31.492918729407737	35.0	33.0	38.5	2.0	40.5
72-73	31.149426181518713	35.0	33.0	37.0	2.0	39.5
74-75	30.819758138337523	35.0	33.0	37.0	2.0	39.0
76-77	30.520868909954203	35.0	33.0	36.0	2.0	39.0
78-79	30.26135999385161	35.0	32.0	36.0	2.0	37.5
80-81	30.03451096244171	35.0	32.0	35.5	2.0	37.0
82-83	29.829399363541672	35.0	32.0	35.0	2.0	37.0
84-85	29.65612442741557	35.0	32.0	35.0	2.0	36.0
86-87	29.513753273361537	35.0	32.0	35.0	2.0	36.0
88-89	29.394355790577343	35.0	32.0	35.0	2.0	36.0
90-91	29.290736050996646	35.0	32.0	35.0	2.0	35.5
92-93	29.21762300997426	35.0	31.0	35.0	2.0	35.0
94-95	29.14662379039489	35.0	31.0	35.0	2.0	35.0
96-97	29.083163846467706	35.0	31.0	35.0	2.0	35.0
98-99	29.0244620315055	35.0	31.0	35.0	2.0	35.0
100-101	28.970603948091096	35.0	31.0	35.0	2.0	35.0
102-103	28.305902079392958	34.0	29.5	35.0	2.0	35.0
104-105	28.841166282079882	35.0	31.0	35.0	2.0	35.0
106-107	28.892969278670147	35.0	31.0	35.0	2.0	35.0
108-109	28.965681174742365	35.0	31.5	35.0	2.0	35.0
110-111	29.07261473401653	35.0	32.0	35.0	2.0	35.0
112-113	29.03692814194283	35.0	32.0	35.0	2.0	35.0
114-115	28.979387897794265	35.0	32.0	35.0	2.0	35.0
116-117	28.921309357112108	35.0	32.0	35.0	2.0	35.0
118-119	28.847982934469037	35.0	32.0	35.0	2.0	35.0
120-121	28.756796188998006	35.0	32.0	35.0	2.0	35.0
122-123	28.646664717177188	35.0	31.5	35.0	2.0	35.0
124-125	28.132427195628146	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110308.0
3	7349.0
4	5295.0
5	5017.0
6	5264.0
7	5433.0
8	5868.0
9	5647.0
10	5882.0
11	6028.0
12	6805.0
13	8700.0
14	10790.0
15	9600.0
16	6181.0
17	5252.0
18	4674.0
19	4031.0
20	3777.0
21	3796.0
22	4350.0
23	5292.0
24	7010.0
25	9173.0
26	10611.0
27	12096.0
28	14245.0
29	16722.0
30	20274.0
31	25268.0
32	32498.0
33	43394.0
34	67259.0
35	114234.0
36	250013.0
37	546951.0
38	194975.0
39	357.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80500459266365	18.20889252540072	12.497562596594845	26.488540285340783
2	15.782366992643801	19.548693185971924	38.505541361355995	26.16339846002828
3	19.152796861321942	23.313082386550022	28.677677533195993	28.856443218932043
4	13.503502521352912	15.913989649610377	31.59312382158233	38.98938400745438
5	14.00388723877724	37.87510132798342	31.83385653522458	16.287154898014755
6	33.83298929630153	33.847742671136025	16.583981085673347	15.7352869468891
7	29.8030595733697	32.0738337540554	18.45637604865418	19.66673062392072
8	27.95350060833142	35.471689624121794	17.890317875820404	18.684491891726378
9	27.80898700876705	16.57322643737776	17.930588448488106	37.68719810536709
10-11	26.952089118388788	25.6021664746299	25.241410208024558	22.20433419895675
12-13	26.294775011331488	22.835935668401557	26.343226895484595	24.526062424782356
14-15	24.01330526568355	23.923110135533257	23.973378221578223	28.090206377204968
16-17	23.919892228222732	27.004396973542555	24.610117725420654	24.465593072814055
18-19	23.91511222998477	25.9943489798609	26.65370756033264	23.43683122982169
20-21	24.77532446190654	25.02288463208697	26.50462160221792	23.69716930378857
22-23	24.6140541945578	24.33700174766733	26.459352207140757	24.589591850634115
24-25	24.21784545172233	24.67078933704236	26.419019019394298	24.69234619184101
26-27	23.783990317535594	24.951372109428842	26.639086389251815	24.62555118378375
28-29	23.862782183915588	25.625945455533834	26.379779295297045	24.131493065253537
30-31	24.87061200848028	25.03197600128466	26.322950427356833	23.774461562878223
32-33	24.359339647929698	25.10954943674125	26.396087524579503	24.135023390749545
34-35	24.30001143450559	25.446961077067943	26.506339902238103	23.74668758618837
36-37	24.380865260909797	25.29931224260647	26.629026523679112	23.690795972804622
38-39	24.30647849094518	25.438150884237192	26.531489566169856	23.723881058647766
40-41	24.22474989362161	25.298250020775807	26.55501465553708	23.9219854300655
42-43	24.023615065804645	25.449960291648626	26.152932450814443	24.37349219173229
44-45	23.731160402369632	25.571615933077524	26.553108904605605	24.14411475994724
46-47	23.7408141243012	25.437619773321863	26.063549607946417	24.75801649443052
48-49	23.90708308261774	25.79327663568103	25.767064749918617	24.532575531782612
50-51	23.93548189567857	25.899311367835548	25.948517232049856	24.216689504436026
52-53	24.082193475583583	25.786278468326106	25.82223780147574	24.309290254614574
54-55	23.68901518914734	25.4025352110916	26.262934893924655	24.645514705836412
56-57	23.882402045964213	25.673807921550544	25.774656511825967	24.66913352065928
58-59	23.831852783552307	25.81511466684662	26.07767090993046	24.275361639670614
60-61	24.419074004994943	25.577426911327596	26.191266162173783	23.812232921503682
62-63	24.14605175269726	25.64297224664291	26.145309447088543	24.065666553571283
64-65	23.964692996021668	25.241577361928346	26.078733131761123	24.714996510288866
66-67	23.85715865657681	25.585331091420432	25.954859321215256	24.6026509307875
68-69	23.55351942210134	25.569116587593626	26.175582769262302	24.70178122104274
70-71	23.719694654962233	25.5760210294929	26.20707452235946	24.49720979318541
72-73	23.71104067122422	26.036806611268677	25.660780083215705	24.591372634291396
74-75	23.593665158936503	25.983164408820443	25.81295898136676	24.610211450876303
76-77	23.58335535881541	25.421498994950696	25.909558684319546	25.08558696191435
78-79	23.458106908253402	25.475078713761835	26.223413993460465	24.8434003845243
80-81	23.789051492140494	25.784029057390594	25.656093810433394	24.770825640035515
82-83	23.862219831181708	25.809584864963487	25.834297143435563	24.49389816041924
84-85	23.8396007545524	25.91321397708975	25.557244696545094	24.68994057181276
86-87	23.409338429498774	26.247032808283333	25.596703113372186	24.746925648845707
88-89	23.19017707237917	25.877004709391727	25.861914911032674	25.070903307196428
90-91	23.675673277652013	25.469705707949863	25.985258841914327	24.869362172483797
92-93	23.66903355127667	25.770674624597607	25.79204406854218	24.76824775558355
94-95	23.708719755938684	25.696263631208733	25.541210340426794	25.05380627242579
96-97	23.265450982744486	25.652486603483094	25.69891203287145	25.383150380900965
98-99	23.13379281731622	25.715273848683957	25.612300782576558	25.53863255142327
100-101	23.215857847226257	25.852917267290625	25.715578232950246	25.215646652532868
102-103	23.04952640527262	25.760222791656435	25.72941835856735	25.460832444503595
104-105	23.32295480121143	25.86678863472628	25.55930665656931	25.250949907492977
106-107	23.45509843525205	25.553488386624117	25.662616161095464	25.32879701702837
108-109	23.26595085277828	25.572632365584948	25.707503549077238	25.453913232559533
110-111	23.05357577454105	26.088693842317824	25.649026768361093	25.20870361478004
112-113	23.067509617853936	26.186743513689315	25.601580595960154	25.144166272496594
114-115	23.0915442662756	26.21751081124482	25.506196197616	25.18474872486358
116-117	22.816569363566806	25.986972725452173	25.63434466698096	25.562113244000056
118-119	22.89232209240532	25.718773831686935	25.75367099751846	25.63523307838928
120-121	22.79494119187099	25.81734192318062	25.926126105298213	25.461590779650173
122-123	22.718638484422264	26.033105618432472	25.586746505389613	25.66150939175565
124-125	22.502642280931244	25.99429585985898	25.407823525938404	26.09523833327137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	55.0
2	72.0
3	87.0
4	76.5
5	94.0
6	224.0
7	623.5
8	922.0
9	1061.0
10	1063.0
11	897.0
12	932.5
13	1049.0
14	1140.5
15	1225.5
16	1317.5
17	1409.5
18	1490.5
19	1616.0
20	1730.0
21	1833.5
22	1942.5
23	2087.5
24	2371.0
25	2752.5
26	3207.0
27	3872.5
28	4657.0
29	5701.5
30	7246.5
31	8990.0
32	11228.0
33	13803.5
34	16315.0
35	18816.5
36	21860.5
37	25410.5
38	28370.5
39	31493.5
40	35137.0
41	37999.0
42	40816.5
43	44761.5
44	49160.0
45	53478.0
46	57488.0
47	62060.0
48	67385.5
49	72034.5
50	75506.5
51	77438.0
52	76603.5
53	74610.0
54	72531.0
55	69757.0
56	65902.0
57	59713.5
58	53258.5
59	46981.5
60	39848.5
61	33048.0
62	26801.0
63	20477.5
64	14774.5
65	10753.5
66	8020.0
67	5974.5
68	4399.5
69	3364.5
70	2575.0
71	1897.5
72	1405.5
73	1040.0
74	792.5
75	602.0
76	438.0
77	307.5
78	250.5
79	204.0
80	167.5
81	146.5
82	141.5
83	143.5
84	126.5
85	107.5
86	100.0
87	97.0
88	103.5
89	88.5
90	75.0
91	76.5
92	71.5
93	63.0
94	56.0
95	53.0
96	47.0
97	37.0
98	29.0
99	15.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3426602658428824
2	0.0
3	0.0
4	0.017870320209895034
5	0.1802028093892912
6	0.04904965512156503
7	0.024493585742233752
8	0.008747709193654911
9	0.006810716443631324
10-11	0.0019369927500235875
12-13	0.05586037156519637
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	1.2496727419507018E-4
92-93	1.8745091129260524E-4
94-95	9.372545564630262E-5
96-97	1.8745091129260524E-4
98-99	3.1241818548767546E-5
100-101	0.0
102-103	0.0
104-105	0.0
106-107	9.372545564630262E-5
108-109	1.8745091129260524E-4
110-111	2.1869272984137282E-4
112-113	6.248363709753508E-4
114-115	1.2496727419507018E-4
116-117	4.6862727823151313E-4
118-119	1.562090927438377E-4
120-121	1.562090927438377E-4
122-123	0.001624574564535912
124-125	1.562090927438377E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1600419.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.07968418156737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.80088974981658	52.230539753712094
2	9.319710349744966	11.757687710511947
3	2.978984325622317	5.637401713262857
4	1.4444104263442554	3.6445181408943483
5	0.8228580601329027	2.5952813279720344
6	0.5329164416390061	2.016972050025191
7	0.37225206458818005	1.6437079869111175
8	0.27557153619581687	1.3906372378129193
9	0.21330643943738062	1.210977255024414
>10	1.1657892685678373	13.073756126242634
>50	0.05558998778369686	2.339801284867107
>100	0.017028410206204693	1.7241343920106276
>500	9.899141725669492E-5	0.03247060615363799
>1k	5.939485035401696E-4	0.7021144145990491
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3142	0.19632358776045522	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2458	0.15358477998574124	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2355	0.1471489653646951	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.248363709753508E-5	1.2496727419507016E-4
2	0.0	0.0	0.0	6.248363709753508E-5	1.2496727419507016E-4
3	0.0	0.0	0.0	6.248363709753508E-5	1.2496727419507016E-4
4	0.0	0.0	0.0	6.248363709753508E-5	1.2496727419507016E-4
5	0.0	0.0	0.0	6.248363709753508E-5	1.2496727419507016E-4
6	0.0	0.0	0.0	6.248363709753508E-5	1.8745091129260524E-4
7	0.0	0.0	0.0	6.248363709753508E-5	1.8745091129260524E-4
8	0.0	0.0	0.0	6.248363709753508E-5	1.8745091129260524E-4
9	0.0	0.0	0.0	6.248363709753508E-5	1.8745091129260524E-4
10-11	0.0	0.0	0.0	6.248363709753508E-5	2.499345483901403E-4
12-13	0.0	0.0	0.0	6.248363709753508E-5	4.998690967802806E-4
14-15	0.0	0.0	0.0	6.248363709753508E-5	5.311109153290482E-4
16-17	0.0	0.0	0.0	6.248363709753508E-5	5.623527338778157E-4
18-19	0.0	0.0	0.0	6.248363709753508E-5	5.935945524265832E-4
20-21	0.0	0.0	0.0	6.248363709753508E-5	6.87320008072886E-4
22-23	0.0	0.0	0.0	1.2496727419507016E-4	7.49803645170421E-4
24-25	0.0	0.0	0.0	1.8745091129260524E-4	8.12287282267956E-4
26-27	0.0	0.0	0.0	5.311109153290482E-4	8.12287282267956E-4
28-29	0.0	0.0	0.0	0.0019369927500235877	8.12287282267956E-4
30-31	0.0	0.0	0.0	0.007091892810570231	8.435291008167237E-4
32-33	0.0	0.0	0.0	0.020338423875247667	0.0010622218306580965
34-35	0.0	0.0	0.0	0.03770887498836242	0.0011871891048531667
36-37	0.0	0.0	0.0	0.05998429161363368	0.0011871891048531667
38-39	0.0	0.0	0.0	0.10091107391251916	0.0011871891048531667
40-41	0.0	0.0	0.0	0.1551468709131796	0.0011871891048531667
42-43	0.0	0.0	0.0	0.20210332419197724	0.0011871891048531667
44-45	0.0	0.0	0.0	0.2502157247570792	0.0011871891048531667
46-47	0.0	0.0	0.0	0.30163975808835064	0.0011871891048531667
48-49	0.0	0.0	0.0	0.3599994751374484	0.0012184309234019343
50-51	0.0	0.0	0.0	0.42904389413022465	0.0012496727419507017
52-53	0.0	0.0	0.0	0.5006501422439998	0.001280914560499469
54-55	0.0	0.0	0.0	0.5798793940836744	0.001374640016145772
56-57	0.0	0.0	0.0	0.6562031567983135	0.001374640016145772
58-59	0.0	0.0	0.0	0.7491788087994455	0.001374640016145772
60-61	0.0	0.0	0.0	0.8460909299377226	0.001374640016145772
62-63	0.0	0.0	0.0	0.9616231749310649	0.0014371236532433069
64-65	0.0	0.0	0.0	1.065502221605717	0.0014996072903408419
66-67	0.0	0.0	0.0	1.1785663629337066	0.0015308491088896095
68-69	0.0	0.0	0.0	1.306189191705422	0.001562090927438377
70-71	0.0	0.0	0.0	1.441559991477232	0.001562090927438377
72-73	0.0	0.0	0.0	1.5702450420796055	0.0016558163830846797
74-75	0.0	0.0	0.0	1.7015544054400755	0.0016870582016334473
76-77	0.0	0.0	0.0	1.8583883345548884	0.0016870582016334473
78-79	0.0	0.0	0.0	2.0375289221135215	0.0016870582016334473
80-81	0.0	0.0	0.0	2.2052974877204035	0.00178078365727975
82-83	0.0	0.0	0.0	2.3811576843314155	0.0018745091129260525
84-85	0.0	0.0	0.0	2.5845419230838926	0.0018745091129260525
86-87	0.0	0.0	0.0	2.8069836711511176	0.0018745091129260525
88-89	0.0	0.0	0.0	3.0579179577348183	0.0019369927500235875
90-91	0.0	0.0	0.0	3.3133198243709927	0.0019369927500235875
92-93	0.0	0.0	0.0	3.543634510712507	0.0019369927500235875
94-95	0.0	0.0	0.0	3.784071546263822	0.0019994763871211225
96-97	0.0	0.0	0.0	4.067809742323729	0.002124443661316193
98-99	0.0	0.0	0.0	4.395348967989008	0.0021869272984137277
100-101	0.0	0.0	0.0	4.731510935573747	0.0021869272984137277
102-103	0.0	0.0	0.0	5.073171463223069	0.002218169116962495
104-105	0.0	0.0	0.0	5.433389631090359	0.002249410935511263
106-107	0.0	0.0	0.0	5.835221901264607	0.002249410935511263
108-109	0.0	0.0	0.0	6.258048673503627	0.002249410935511263
110-111	0.0	0.0	0.0	6.714272949771279	0.002311894572608798
112-113	0.0	0.0	0.0	7.153595402204048	0.002311894572608798
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2320	0.0	72.12111	1
GTATCAA	4145	0.0	52.04452	1
GTACATG	8270	0.0	41.042633	1
TACATGG	8595	0.0	39.246956	2
ACATGGG	8845	0.0	37.59861	3
TCAACGC	5620	0.0	36.586273	4
TATCAAC	5780	0.0	36.50151	2
CAACGCA	5785	0.0	35.33672	5
CATGGGG	8155	0.0	34.71403	4
ATCAACG	6005	0.0	34.538353	3
AACGCAG	6010	0.0	33.60346	6
ATGGGGG	5245	0.0	33.179726	5
GTGGTAT	1135	0.0	33.169357	1
TGGTATC	1145	0.0	32.792194	2
ACGCAGA	7280	0.0	27.326168	7
CGCAGAG	7495	0.0	26.537317	8
TGGGGGG	4100	0.0	26.445166	6
GTAAGTA	770	0.0	24.768211	2
AGAGTAC	7745	0.0	24.140686	10-11
GCAGAGT	8405	0.0	23.520977	9
>>END_MODULE