FastQCFastQC Report
Mon 15 Aug 2016
SRR2921304_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921304_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123340
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18800.049.697981
GGTATCA11900.048.0088351
GTACATG60000.041.8560371
TACATGG61050.040.45382
ACATGGG62200.039.418833
TCAACGC24250.038.2833374
CAACGCA25200.037.7847335
AACGCAG26850.035.906056
ATCAACG26450.035.324083
CATGGGG54200.035.135624
TATCAAC27000.034.824932
ATGGGGG34950.032.5224385
ACGCAGA32800.028.6592047
CGCAGAG35450.026.515668
TGGGGGG30100.025.7024066
AGTACAT39600.025.6979142
GAGTACA40850.025.4942871
GTATAGG3103.3651304E-1023.0364971
AGAGTAC36400.022.47209510-11
GCAGAGT41200.022.2374539
Produced by FastQC (version 0.11.5)