##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921304_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1123340
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.11756369398401	31.0	31.0	34.0	26.0	34.0
2	30.070695426139903	31.0	31.0	34.0	26.0	34.0
3	30.18534459736144	33.0	31.0	34.0	26.0	34.0
4	33.59229885876048	37.0	35.0	37.0	28.0	37.0
5	33.61154859615076	37.0	35.0	37.0	28.0	37.0
6	33.77061085690886	37.0	35.0	37.0	29.0	37.0
7	33.6201506222515	37.0	35.0	37.0	28.0	37.0
8	33.64553474460092	37.0	35.0	37.0	28.0	37.0
9	35.20985632132747	39.0	37.0	39.0	28.0	39.0
10-11	35.23024062171738	39.0	37.0	39.0	28.0	39.0
12-13	35.149926558299356	39.0	37.0	39.0	27.0	39.0
14-15	36.42749078640483	40.0	38.0	41.0	27.0	41.0
16-17	36.4152651913045	40.0	38.0	41.0	27.0	41.0
18-19	36.40611346520198	40.0	38.0	41.0	27.0	41.0
20-21	36.46928267488027	40.0	38.0	41.0	27.0	41.0
22-23	36.426891235066854	40.0	38.0	41.0	27.0	41.0
24-25	36.447009364929585	40.0	38.0	41.0	27.0	41.0
26-27	36.43835659729022	40.0	38.0	41.0	28.0	41.0
28-29	36.38369772286218	40.0	37.5	41.0	28.5	41.0
30-31	36.30051275660085	40.0	37.0	41.0	28.0	41.0
32-33	36.223163067281504	40.0	37.0	41.0	28.0	41.0
34-35	36.110461213880036	40.0	37.0	41.0	27.0	41.0
36-37	35.9970485338366	40.0	37.0	41.0	27.0	41.0
38-39	35.889662969359236	40.0	36.0	41.0	26.5	41.0
40-41	35.79059768191287	40.0	36.0	41.0	26.0	41.0
42-43	35.57251900582192	40.0	35.5	41.0	25.5	41.0
44-45	35.28486255274449	40.0	35.0	41.0	24.0	41.0
46-47	35.04391546637706	40.0	35.0	41.0	23.5	41.0
48-49	34.82481795360265	39.0	35.0	41.0	23.0	41.0
50-51	34.6406791354354	39.0	35.0	41.0	22.5	41.0
52-53	34.45883036302455	39.0	34.5	41.0	22.0	41.0
54-55	34.257840457920125	39.0	34.0	40.5	20.5	41.0
56-57	34.01631384976944	38.0	34.0	40.0	19.5	41.0
58-59	33.75575115281215	38.0	34.0	40.0	18.0	41.0
60-61	33.477271796606544	37.5	33.5	40.0	16.0	41.0
62-63	33.14678592411914	37.0	33.0	40.0	13.0	41.0
64-65	32.80445991418449	36.5	33.0	39.5	10.0	41.0
66-67	32.46378968077341	36.0	33.0	39.0	8.0	41.0
68-69	32.103511848594366	35.5	32.0	39.0	7.0	40.0
70-71	32.05899237986718	35.0	33.0	38.5	6.0	40.0
72-73	31.764164010896078	35.0	33.0	37.5	2.0	39.5
74-75	31.433405291363258	35.0	32.0	37.0	2.0	39.0
76-77	31.131398775081454	35.0	32.0	36.5	2.0	39.0
78-79	30.836548596150763	35.0	32.0	36.0	2.0	38.0
80-81	30.56506979187067	35.0	32.0	36.0	2.0	37.0
82-83	30.33814383890897	35.0	32.0	35.0	2.0	37.0
84-85	30.120498691402425	35.0	31.0	35.0	2.0	36.0
86-87	29.948589474246443	35.0	31.0	35.0	2.0	36.0
88-89	29.80400145993199	35.0	31.0	35.0	2.0	36.0
90-91	29.67915234924422	35.0	31.0	35.0	2.0	35.5
92-93	29.568510424270478	35.0	31.0	35.0	2.0	35.0
94-95	29.444142022896006	35.0	31.0	35.0	2.0	35.0
96-97	29.346833104848045	35.0	31.0	35.0	2.0	35.0
98-99	29.261857051293465	34.0	31.0	35.0	2.0	35.0
100-101	29.173309950682786	34.0	31.0	35.0	2.0	35.0
102-103	28.323362027525057	33.5	29.0	34.5	2.0	35.0
104-105	28.927849983086155	34.0	30.0	35.0	2.0	35.0
106-107	29.005227268680898	34.0	30.0	35.0	2.0	35.0
108-109	28.943118735200386	34.0	30.0	35.0	2.0	35.0
110-111	28.86751028183809	34.0	30.0	35.0	2.0	35.0
112-113	28.779512436127977	34.0	30.0	35.0	2.0	35.0
114-115	28.648873003721043	34.0	30.0	35.0	2.0	35.0
116-117	28.51606904410063	34.0	29.5	35.0	2.0	35.0
118-119	28.5116051240052	34.0	29.5	35.0	2.0	35.0
120-121	28.665582548471523	35.0	30.5	35.0	2.0	35.0
122-123	28.52927297167376	35.0	30.5	35.0	2.0	35.0
124-125	27.95265502875354	34.5	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48312.0
3	6422.0
4	3900.0
5	3276.0
6	3389.0
7	3784.0
8	4073.0
9	4319.0
10	4927.0
11	5546.0
12	5971.0
13	5971.0
14	6265.0
15	5546.0
16	4057.0
17	3605.0
18	3303.0
19	3151.0
20	3105.0
21	3263.0
22	3650.0
23	4841.0
24	7457.0
25	9592.0
26	9580.0
27	10092.0
28	11998.0
29	14425.0
30	17275.0
31	21873.0
32	28535.0
33	38736.0
34	62546.0
35	100085.0
36	206672.0
37	348241.0
38	95482.0
39	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.36889331293838	18.13963178691435	12.297156044496123	25.194318855651137
2	16.346520232613628	19.89261384072448	39.13051219868309	24.630353727978804
3	20.6519716640893	23.983230639249527	28.15349182142002	27.211305875241155
4	13.423912849933938	14.88534666652422	34.61937728585521	37.07136319768664
5	13.93490054893498	38.6252232329653	32.25362469797893	15.186251520120791
6	33.867133029034264	34.038100903102176	17.24024013862645	14.85452592923711
7	29.479365316780715	32.68242869072993	18.99063929501801	18.847566697471347
8	27.957486095642043	35.708581394914034	18.40298644330663	17.93094606613729
9	28.21255858011766	16.530721621583123	17.961037989829496	37.29568180846972
10-11	26.700155574300638	26.12473097219471	26.002764281565	21.17234917193965
12-13	25.83708799940175	23.444517742410767	26.673886664725906	24.044507593461585
14-15	23.785281335951275	24.752898779416785	23.94463686302858	27.51718302160336
16-17	24.195990088058174	27.79020405656056	24.503123187525233	23.510682667856027
18-19	23.991798984309725	27.181399398455675	26.005730562092737	22.821071055141857
20-21	24.840055818549654	25.996443459236655	25.943829802387203	23.21967091982649
22-23	24.716879005557814	25.401835512511095	25.943284105608296	23.938001376322795
24-25	24.19246500982169	25.516133785046375	26.017878898350766	24.27352230678117
26-27	23.760369664605307	25.95646621504627	26.092584407300528	24.190579713047892
28-29	24.02983765824771	26.631107067392456	25.850712890881468	23.488342383478365
30-31	24.94983450282298	25.954346281699518	25.834340797769396	23.26147841770811
32-33	24.382521371991732	25.889967349427888	25.91075510290383	23.81675617567655
34-35	24.195099890007704	26.2469391561441	26.0959501380134	23.462010815834795
36-37	24.190230733106763	26.32867949976297	26.031199157814143	23.449890609316125
38-39	24.274718979338477	26.40870884732676	25.896366105612046	23.420206067722717
40-41	24.192335050642367	26.060097803820813	26.081107909147317	23.666459236389503
42-43	23.98719448465457	26.071384694275444	25.844857762987573	24.096563058082413
44-45	23.621407236938747	26.163995139170186	26.318989730829323	23.895607893061744
46-47	23.561066374655574	25.989788514527106	26.121994756264215	24.327150354553105
48-49	23.581157295258343	26.2586389401535	26.06184515208897	24.098358612499187
50-51	23.75651161351807	26.198063441799764	26.236077065277048	23.809347879405117
52-53	23.88100626198493	26.210822533174987	26.02480579300374	23.883365411836344
54-55	23.572133461470493	25.803420443159315	26.382441351209028	24.24200474416116
56-57	23.806613497189215	26.141239344107593	25.88105996500376	24.17108719369943
58-59	23.743587346736195	26.43773633617994	26.146220735488207	23.67245558159566
60-61	24.278400924430233	26.281496305706515	26.14395347936613	23.296149290497127
62-63	24.123327890948133	26.246570318050143	26.04573027966263	23.584371511339093
64-65	23.829085653469978	25.877826884103484	26.103456825183308	24.18963063724323
66-67	23.876132547254848	26.186384283771662	25.90511418390943	24.032368985064064
68-69	23.557208659685884	26.13618760872236	26.20883239530159	24.097771336290165
70-71	23.638039720855453	26.065910019594245	26.39124926888806	23.904800990662242
72-73	23.71948038536661	26.61720796158441	25.782965100855165	23.880346552193814
74-75	23.616380210176406	26.484149971356572	25.94266240954621	23.956807408920813
76-77	23.519962111314925	26.031689932390595	26.015086925421336	24.433261030873137
78-79	23.499437151224043	26.09472862854963	26.296098961678656	24.10973525854767
80-81	23.84399058491735	26.367661826184186	25.87278243467485	23.91556515422361
82-83	23.884876843256027	26.465524301957476	26.01866491466094	23.63093394012556
84-85	23.907907932860624	26.63616279789236	25.60847116681082	23.8474581024362
86-87	23.451561225121154	26.8577815544547	25.764098254335448	23.926558966088695
88-89	23.362509558982595	26.325282273751455	26.078506955912506	24.233701211353445
90-91	23.930213614532374	26.0357789875232	26.114476735645898	23.919530662298527
92-93	23.941359813471006	26.309606795239205	25.967343181480285	23.781690209809508
94-95	23.902725209354998	26.322353945595523	25.786018785307025	23.988902059742458
96-97	23.535468282464258	26.24147223708494	25.96027751786568	24.262781962585123
98-99	23.330321294078896	26.2630088936765	25.899071461002254	24.507598351242354
100-101	23.305147366490573	26.534247819282193	25.992295598324155	24.16830921590308
102-103	23.19916423629287	26.515823246836952	25.998767898412154	24.28624461845802
104-105	23.575631161047887	26.53524878325842	25.813794693609026	24.07532536208467
106-107	23.53538698758798	26.33962035706591	25.982581151748562	24.142411503597543
108-109	23.401353458494636	26.432902849313678	26.001306007769276	24.164437684422406
110-111	23.204946670874655	26.884591775460816	25.94420439426117	23.966257159403362
112-113	23.21554795483964	27.087357983630174	25.871772638491	23.82532142303919
114-115	23.385810614419956	27.14077973919078	25.740945410066573	23.732464236322695
116-117	23.173814402061062	26.927872184256362	25.819935734255495	24.07837767942708
118-119	23.142776009739542	26.703717457038458	25.97258324725942	24.18092328596259
120-121	23.14407506229818	26.83455792996429	26.09957737839279	23.921789629344737
122-123	23.103759853647464	26.959000814553352	25.89683214414479	24.0404071876544
124-125	23.053712305865304	26.83411999095537	25.803601473491923	24.308566229687408
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	378.0
1	322.5
2	262.0
3	234.0
4	220.0
5	217.0
6	204.0
7	271.0
8	367.0
9	417.0
10	434.5
11	455.5
12	496.5
13	510.0
14	548.0
15	592.0
16	616.5
17	679.0
18	730.5
19	776.5
20	841.0
21	948.5
22	1105.0
23	1284.5
24	1583.0
25	1981.5
26	2579.5
27	3394.5
28	4172.5
29	5142.5
30	6389.5
31	7826.0
32	9684.5
33	11693.0
34	13625.0
35	15729.5
36	18100.5
37	20501.0
38	22619.5
39	24636.0
40	26844.0
41	28603.5
42	30299.0
43	33053.0
44	36338.5
45	39376.0
46	41974.0
47	44955.5
48	48560.0
49	51424.5
50	52644.5
51	52536.0
52	51346.5
53	49620.0
54	48356.5
55	46258.0
56	42810.5
57	38616.5
58	34642.0
59	30372.0
60	25244.0
61	21017.5
62	17059.0
63	12799.5
64	9355.5
65	7005.0
66	5215.5
67	3829.0
68	2766.0
69	2073.5
70	1593.5
71	1149.0
72	867.0
73	689.0
74	484.0
75	317.0
76	248.5
77	161.5
78	93.0
79	68.0
80	47.5
81	41.5
82	35.5
83	31.0
84	26.0
85	20.0
86	15.5
87	12.5
88	11.0
89	9.0
90	9.0
91	9.0
92	9.0
93	5.0
94	1.5
95	1.5
96	2.5
97	3.0
98	1.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016557765235814623
2	0.009258105293143661
3	0.008991044563533748
4	0.011394591130022967
5	0.006587497997044527
6	0.028664518311464028
7	0.011928712589242794
8	0.01166165185963288
9	0.008189862374704008
10-11	0.007344170064272616
12-13	0.006320437267434615
14-15	0.00583082592981644
16-17	0.004317481795360264
18-19	0.005741805686613135
20-21	0.0051186639841900045
22-23	0.005074153862588353
24-25	0.005385724713799918
26-27	0.004584542524970178
28-29	0.0054747449570032225
30-31	0.005163174105791657
32-33	0.007344170064272616
34-35	0.007077109334662702
36-37	0.006453967632239571
38-39	0.006097886659426354
40-41	0.006320437267434615
42-43	0.006587497997044527
44-45	0.006676518240247832
46-47	0.00845692310431392
48-49	0.007388680185874268
50-51	0.00520768422739331
52-53	0.00480709313297844
54-55	0.005741805686613135
56-57	0.006810048605052788
58-59	0.006453967632239571
60-61	0.0047625830113767875
62-63	0.005163174105791657
64-65	0.0036053198497338294
66-67	0.0037833603361404388
68-69	0.005875336051418092
70-71	0.0045400324033685255
72-73	0.004094931187352004
74-75	0.004050421065750352
76-77	0.004406502038563569
78-79	0.003249238876920612
80-81	0.0032047287553189593
82-83	0.0049851336193850486
84-85	0.008412412982712269
86-87	0.006364947389036268
88-89	0.0045400324033685255
90-91	0.004896113376181744
92-93	0.007388680185874268
94-95	0.006097886659426354
96-97	0.008634963590720529
98-99	0.00623141702423131
100-101	0.007655740915484181
102-103	0.0049851336193850486
104-105	0.004718072889775135
106-107	0.006587497997044527
108-109	0.0061869069026296575
110-111	0.007077109334662702
112-113	0.00480709313297844
114-115	0.0025370769312941764
116-117	0.0031602186337173076
118-119	0.1536934498905051
120-121	0.0100592874819734
122-123	0.0022255060800826106
124-125	0.0019584453504726974
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1123340.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.9675502782224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.84186097757022	59.36177173646101
2	8.862255838698264	12.40140661945182
3	2.56367661108414	5.381225165493971
4	1.1612897813999536	3.250104046707486
5	0.6611691514024247	2.3130192921579393
6	0.4240042388110471	1.7799922738314815
7	0.2971800338001829	1.455507126961869
8	0.2074987034844193	1.1614540774969655
9	0.15918447329693874	1.0023972875024323
>10	0.784687891891666	9.358840469398956
>50	0.02955904407198154	1.420904462949172
>100	0.007124372383019894	0.8383270064865233
>500	5.088821057134016E-4	0.27505043510029364
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7804048640660886E-4	0.0
4	0.0	0.0	0.0	1.7804048640660886E-4	0.0
5	0.0	0.0	0.0	1.7804048640660886E-4	0.0
6	0.0	0.0	0.0	1.7804048640660886E-4	0.0
7	0.0	0.0	0.0	1.7804048640660886E-4	0.0
8	0.0	0.0	0.0	1.7804048640660886E-4	0.0
9	0.0	0.0	0.0	1.7804048640660886E-4	0.0
10-11	0.0	0.0	0.0	2.670607296099133E-4	0.0
12-13	0.0	0.0	0.0	2.670607296099133E-4	4.4510121601652215E-5
14-15	0.0	0.0	0.0	2.670607296099133E-4	8.902024320330443E-5
16-17	0.0	0.0	0.0	3.115708512115655E-4	8.902024320330443E-5
18-19	0.0	0.0	0.0	3.560809728132177E-4	8.902024320330443E-5
20-21	0.0	0.0	0.0	4.4510121601652215E-4	8.902024320330443E-5
22-23	0.0	0.0	0.0	4.896113376181743E-4	8.902024320330443E-5
24-25	0.0	0.0	0.0	6.231417024231311E-4	8.902024320330443E-5
26-27	0.0	0.0	0.0	0.001023732796838001	8.902024320330443E-5
28-29	0.0	0.0	0.0	0.0028041376609040892	8.902024320330443E-5
30-31	0.0	0.0	0.0	0.012373813805259317	8.902024320330443E-5
32-33	0.0	0.0	0.0	0.03169120658037638	8.902024320330443E-5
34-35	0.0	0.0	0.0	0.05461391920522727	8.902024320330443E-5
36-37	0.0	0.0	0.0	0.08496982213755408	8.902024320330443E-5
38-39	0.0	0.0	0.0	0.14145316645005074	8.902024320330443E-5
40-41	0.0	0.0	0.0	0.2109779763918315	8.902024320330443E-5
42-43	0.0	0.0	0.0	0.2738262680933644	8.902024320330443E-5
44-45	0.0	0.0	0.0	0.34050243025263943	1.3353036480495665E-4
46-47	0.0	0.0	0.0	0.40873644666797226	1.7804048640660886E-4
48-49	0.0	0.0	0.0	0.4860950380116439	2.2255060800826108E-4
50-51	0.0	0.0	0.0	0.5705307386899781	2.670607296099133E-4
52-53	4.4510121601652215E-5	0.0	0.0	0.6556340911923372	2.670607296099133E-4
54-55	8.902024320330443E-5	0.0	0.0	0.7531112574999554	2.670607296099133E-4
56-57	8.902024320330443E-5	0.0	0.0	0.8533925614684779	2.670607296099133E-4
58-59	8.902024320330443E-5	0.0	0.0	0.9641782541349904	2.670607296099133E-4
60-61	8.902024320330443E-5	8.902024320330443E-5	0.0	1.0824861573521818	2.670607296099133E-4
62-63	8.902024320330443E-5	8.902024320330443E-5	0.0	1.224918546477469	2.670607296099133E-4
64-65	8.902024320330443E-5	8.902024320330443E-5	0.0	1.3492353161108837	2.670607296099133E-4
66-67	8.902024320330443E-5	8.902024320330443E-5	0.0	1.4925579076682038	3.560809728132177E-4
68-69	8.902024320330443E-5	8.902024320330443E-5	0.0	1.6484768636387914	3.560809728132177E-4
70-71	8.902024320330443E-5	8.902024320330443E-5	0.0	1.8088468317695443	3.560809728132177E-4
72-73	8.902024320330443E-5	8.902024320330443E-5	0.0	1.9602257553367637	3.560809728132177E-4
74-75	8.902024320330443E-5	8.902024320330443E-5	0.0	2.120818274075525	3.560809728132177E-4
76-77	8.902024320330443E-5	8.902024320330443E-5	0.0	2.3136361208538823	3.560809728132177E-4
78-79	8.902024320330443E-5	8.902024320330443E-5	0.0	2.5291986397706836	3.560809728132177E-4
80-81	8.902024320330443E-5	8.902024320330443E-5	0.0	2.727046130290028	4.4510121601652215E-4
82-83	8.902024320330443E-5	8.902024320330443E-5	0.0	2.9313475884416116	5.341214592198266E-4
84-85	8.902024320330443E-5	8.902024320330443E-5	0.0	3.1553225203411257	5.341214592198266E-4
86-87	8.902024320330443E-5	8.902024320330443E-5	0.0	3.4013299624334574	5.341214592198266E-4
88-89	8.902024320330443E-5	8.902024320330443E-5	0.0	3.6800523439030037	5.341214592198266E-4
90-91	8.902024320330443E-5	8.902024320330443E-5	0.0	3.968032830665693	5.341214592198266E-4
92-93	8.902024320330443E-5	8.902024320330443E-5	0.0	4.245019317392775	5.341214592198266E-4
94-95	8.902024320330443E-5	8.902024320330443E-5	0.0	4.52289600655189	5.341214592198266E-4
96-97	8.902024320330443E-5	8.902024320330443E-5	0.0	4.840965335517296	5.786315808214788E-4
98-99	8.902024320330443E-5	8.902024320330443E-5	0.0	5.208440899460538	6.231417024231311E-4
100-101	8.902024320330443E-5	8.902024320330443E-5	0.0	5.588245767087436	6.231417024231311E-4
102-103	8.902024320330443E-5	8.902024320330443E-5	0.0	5.988124699556679	6.231417024231311E-4
104-105	8.902024320330443E-5	8.902024320330443E-5	0.0	6.397528798048676	6.231417024231311E-4
106-107	8.902024320330443E-5	8.902024320330443E-5	0.0	6.8538910748304165	6.676518240247833E-4
108-109	8.902024320330443E-5	8.902024320330443E-5	0.0	7.3239179589438645	7.121619456264354E-4
110-111	8.902024320330443E-5	8.902024320330443E-5	0.0	7.830532163013869	7.121619456264354E-4
112-113	8.902024320330443E-5	8.902024320330443E-5	0.0	8.317205832606334	7.121619456264354E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1880	0.0	49.69798	1
GGTATCA	1190	0.0	48.008835	1
GTACATG	6000	0.0	41.856037	1
TACATGG	6105	0.0	40.4538	2
ACATGGG	6220	0.0	39.41883	3
TCAACGC	2425	0.0	38.283337	4
CAACGCA	2520	0.0	37.784733	5
AACGCAG	2685	0.0	35.90605	6
ATCAACG	2645	0.0	35.32408	3
CATGGGG	5420	0.0	35.13562	4
TATCAAC	2700	0.0	34.82493	2
ATGGGGG	3495	0.0	32.522438	5
ACGCAGA	3280	0.0	28.659204	7
CGCAGAG	3545	0.0	26.51566	8
TGGGGGG	3010	0.0	25.702406	6
AGTACAT	3960	0.0	25.697914	2
GAGTACA	4085	0.0	25.494287	1
GTATAGG	310	3.3651304E-10	23.036497	1
AGAGTAC	3640	0.0	22.472095	10-11
GCAGAGT	4120	0.0	22.237453	9
>>END_MODULE