FastQCFastQC Report
Mon 15 Aug 2016
SRR2921304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123340
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.23839621129844926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21450.190948421671088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.17394555521925686No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.071.914321
GTATCAA29200.049.113611
GTACATG59000.041.4233061
TACATGG61600.039.5758322
ACATGGG62850.038.3145333
ATGGGGG35100.034.812425
CATGGGG54650.034.683624
TATCAAC43500.032.611832
TCAACGC42850.032.5501144
CAACGCA44350.031.5836075
ATCAACG44450.031.5125523
TGGGGGG27150.030.2940966
AACGCAG45500.030.2586336
GTGGTAT8100.030.2463051
TACCGTA1254.835555E-528.6005127
GTCTTAC3350.028.5396521
TGGTATC8700.026.0346552
ACGCAGA55400.024.6362426
CGCAGAG57900.023.7720137
ACCGCTC3302.910383E-1123.4663648
Produced by FastQC (version 0.11.5)