FastQCFastQC Report
Mon 15 Aug 2016
SRR2921303_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921303_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360973
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.060.6018141
CGTAGCG909.728865E-846.2760473
GTATCAA26750.044.7083851
GTACATG78550.037.267961
TCAACGC32250.036.8978464
CAACGCA33150.035.8947755
TACATGG81250.035.808512
ACATGGG83800.034.647873
AACGCAG35000.034.3361936
CATGGGG70750.034.0587964
ATCAACG35100.033.9018673
TATCAAC36300.032.7811432
ATGGGGG46650.029.8434495
TGGGGGG33650.026.343296
ACGCAGA45250.026.0315157
GTGGTAT9400.025.3191341
CGCAGAG46950.025.0880268
ATGGGGA24400.024.1396055
GAGTACA55400.023.9503461
AGTACAT54950.023.3876612
Produced by FastQC (version 0.11.5)