##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921303_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1360973
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.49112730377458	31.0	31.0	34.0	27.0	34.0
2	30.636523281505216	31.0	31.0	34.0	27.0	34.0
3	30.877011520434277	33.0	31.0	34.0	27.0	34.0
4	34.2483407091838	37.0	35.0	37.0	31.0	37.0
5	34.43049421259643	37.0	35.0	37.0	32.0	37.0
6	34.42347129590374	37.0	35.0	37.0	31.0	37.0
7	34.31672119873061	37.0	35.0	37.0	31.0	37.0
8	34.39427159833443	37.0	35.0	37.0	31.0	37.0
9	35.99179998427596	39.0	37.0	39.0	31.0	39.0
10-11	36.02797961458457	39.0	37.0	39.0	31.5	39.0
12-13	35.96721830631468	39.0	37.0	39.0	31.0	39.0
14-15	37.31409734065261	41.0	38.0	41.0	31.0	41.0
16-17	37.31913858687865	41.0	38.0	41.0	31.0	41.0
18-19	37.31646145808918	41.0	38.0	41.0	31.0	41.0
20-21	37.353244700666366	41.0	38.0	41.0	31.0	41.0
22-23	37.32340575455942	41.0	38.0	41.0	31.0	41.0
24-25	37.324255881637626	41.0	38.0	41.0	31.0	41.0
26-27	37.30745025801394	41.0	38.0	41.0	31.0	41.0
28-29	37.251630634847274	41.0	38.0	41.0	30.0	41.0
30-31	37.17896901701944	41.0	38.0	41.0	30.0	41.0
32-33	37.10219490026621	41.0	38.0	41.0	30.0	41.0
34-35	36.99931152197729	40.0	38.0	41.0	30.0	41.0
36-37	36.89333954457583	40.0	38.0	41.0	30.0	41.0
38-39	36.796482369598806	40.0	38.0	41.0	30.0	41.0
40-41	36.69724674920076	40.0	37.5	41.0	30.0	41.0
42-43	36.510646427225225	40.0	37.0	41.0	29.5	41.0
44-45	36.2826213304746	40.0	36.5	41.0	29.0	41.0
46-47	36.0781672377042	40.0	36.0	41.0	28.0	41.0
48-49	35.88230589438585	40.0	36.0	41.0	28.0	41.0
50-51	35.714973405056526	40.0	35.0	41.0	28.0	41.0
52-53	35.534176651557374	39.0	35.0	41.0	27.0	41.0
54-55	35.33246287766179	39.0	35.0	41.0	27.0	41.0
56-57	35.09586964620165	39.0	35.0	41.0	26.5	41.0
58-59	34.83120238241317	38.5	35.0	40.0	26.0	41.0
60-61	34.55063509709598	38.0	34.5	40.0	26.0	41.0
62-63	34.21658989561145	37.0	34.0	40.0	25.5	41.0
64-65	33.868038160933395	37.0	34.0	40.0	24.5	41.0
66-67	33.52201843827908	36.0	34.0	39.0	23.5	41.0
68-69	33.15958766265018	36.0	33.5	39.0	23.0	40.5
70-71	33.095847970532844	35.5	34.0	39.0	24.0	40.0
72-73	32.79907463263415	35.0	34.0	37.5	23.5	40.0
74-75	32.46577522111019	35.0	33.5	37.0	23.0	39.0
76-77	32.162149800179726	35.0	33.0	37.0	22.0	39.0
78-79	31.86734490691586	35.0	33.0	36.0	21.5	38.5
80-81	31.587935616650732	35.0	33.0	36.0	20.0	37.0
82-83	31.357779324057127	35.0	33.0	35.0	20.0	37.0
84-85	31.136010413138248	35.0	33.0	35.0	19.5	36.5
86-87	30.965710928872213	35.0	33.0	35.0	18.0	36.0
88-89	30.816224127884976	35.0	33.0	35.0	18.0	36.0
90-91	30.692162519021316	35.0	33.0	35.0	17.0	36.0
92-93	30.575928765669858	35.0	32.0	35.0	15.0	35.0
94-95	30.449692242241397	35.0	32.0	35.0	10.5	35.0
96-97	30.355880315039315	35.0	32.0	35.0	8.5	35.0
98-99	30.273189475470858	35.0	32.0	35.0	7.0	35.0
100-101	30.179060863073698	35.0	32.0	35.0	6.0	35.0
102-103	29.3630865564563	34.0	30.5	35.0	2.0	35.0
104-105	29.93974751887069	34.5	31.5	35.0	2.0	35.0
106-107	30.009784543852085	35.0	32.0	35.0	2.0	35.0
108-109	29.94576490496138	35.0	32.0	35.0	2.0	35.0
110-111	29.868714515276938	35.0	31.5	35.0	2.0	35.0
112-113	29.780405636261705	35.0	31.0	35.0	2.0	35.0
114-115	29.651567665192474	35.0	31.0	35.0	2.0	35.0
116-117	29.514697940370603	35.0	31.0	35.0	2.0	35.0
118-119	29.505903864367625	34.5	31.5	35.0	2.0	35.0
120-121	29.66663225501167	35.0	32.0	35.0	2.0	35.0
122-123	29.526669522466648	35.0	32.0	35.0	2.0	35.0
124-125	28.960865131049623	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	39720.0
3	5954.0
4	3853.0
5	3344.0
6	3759.0
7	4257.0
8	4071.0
9	4426.0
10	4988.0
11	5865.0
12	6006.0
13	6006.0
14	5979.0
15	5061.0
16	3951.0
17	3562.0
18	3371.0
19	3398.0
20	3277.0
21	3485.0
22	3900.0
23	5437.0
24	8200.0
25	10075.0
26	10093.0
27	10920.0
28	13042.0
29	15959.0
30	19123.0
31	24034.0
32	31485.0
33	43458.0
34	70277.0
35	116815.0
36	249361.0
37	460243.0
38	144075.0
39	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.370849246774014	18.35565318167154	12.448456619783105	24.825040951771342
2	16.620738813812157	19.636104436263164	39.15316387792744	24.58999287199724
3	20.137944819132972	23.92804706525557	28.244811637569313	27.689196478042145
4	13.752724472264394	15.153283255463274	34.89823002996736	36.19576224230497
5	14.463887860136737	38.28883245448507	32.73327689892951	14.514002786448687
6	33.99273397575591	34.42666688226115	16.958221463068295	14.622377678914638
7	30.243379431318555	32.26903057546423	19.242032426585304	18.245557566631906
8	27.449500590825448	35.46165219487246	18.736956278916644	18.35189093538545
9	28.02591907563723	16.42832315955237	18.260852951572215	37.284904813238185
10-11	26.664055576538985	26.0943798856901	26.045513799341887	21.196050738429026
12-13	26.251024616891566	23.450252025443934	26.481574899081433	23.817148458583066
14-15	23.896590783420635	24.84248295869341	23.849452323103097	27.411473934782855
16-17	24.17319663988055	27.757615526150264	24.527850605185964	23.54133722878322
18-19	24.137041654128875	27.126675976781296	25.99902783885933	22.7372545302305
20-21	25.101688631999025	25.821435827685395	25.892638995895712	23.184236544419864
22-23	24.984219897111526	25.395678272543503	25.68681106716115	23.933290763183827
24-25	24.43524698127869	25.600263064592276	25.785400282537253	24.17908967159178
26-27	23.950725418278797	26.058331058498226	25.90865119484872	24.082292328374255
28-29	24.060782706490393	26.513973857366608	25.72411758416702	23.70112585197598
30-31	24.85582072059329	25.80516095136096	25.761219199900065	23.577799128145685
32-33	24.51481084401713	25.829603464277394	25.660923488879465	23.994662202826007
34-35	24.395859970900755	26.202879061769764	25.886167570506885	23.515093396822596
36-37	24.311551271523495	26.22143595904818	25.86331880216112	23.603693967267205
38-39	24.347228003860767	26.35487825981845	25.8145986812108	23.483295055109988
40-41	24.405629476677646	25.929321078940166	25.87086593343609	23.7941835109461
42-43	24.06242640258982	25.931198982413044	25.753444588429502	24.252930026567633
44-45	23.84274848818527	26.026838087113006	26.07452836046498	24.05588506423674
46-47	23.885166725759962	25.97956780450346	25.831350799229153	24.303914670507424
48-49	23.750005970504606	26.28282261943122	25.90949155917615	24.057679850888025
50-51	23.959955661417535	26.13173056044512	26.00049305914542	23.907820718991925
52-53	24.02905451579481	26.10493867983452	25.880783898772126	23.98522290559854
54-55	23.687913609222228	25.80965635622675	26.19741844651205	24.30501158803897
56-57	23.838587956924457	26.08726802438155	25.67943918022728	24.394704838466712
58-59	23.80033500640583	26.241788797529814	25.991470894159946	23.96640530190441
60-61	24.37509690342577	26.191186509426544	25.909202133014325	23.524514454133357
62-63	24.11339961120931	26.110443449912978	25.981189467306788	23.79496747157092
64-65	23.906677904154613	25.847202005112713	25.928654198149637	24.317465892583037
66-67	24.006495629778495	26.146967297737184	25.6570763052799	24.18946076720442
68-69	23.78226369715672	26.104445263361708	25.983640678372122	24.129650361109448
70-71	23.779300462928944	26.023513851127927	26.10742890734073	24.089756778602396
72-73	23.867786752589723	26.46781651214355	25.632932436627765	24.03146429863896
74-75	23.76248609390454	26.447997448773396	25.776867605153598	24.012648852168468
76-77	23.680913569215754	25.927471739373242	25.883273177241012	24.50834151416999
78-79	23.675103927284884	26.06090331777409	25.97445492136796	24.28953783357306
80-81	23.96173787298079	26.43155993557337	25.605065073244138	24.001637118201696
82-83	24.10522676528844	26.28280988280092	25.816644187496486	23.79531916441416
84-85	24.000465886490506	26.346579132914698	25.557327736816752	24.095627243778043
86-87	23.614910766576358	26.6312187286212	25.632555374543763	24.12131513025868
88-89	23.609568524347317	26.22835117508677	25.852129418814542	24.309950881751373
90-91	24.048898666760724	25.912119662340103	25.94632506819037	24.092656602708804
92-93	24.13388186043128	26.085373439447878	25.828880561616895	23.95186413850395
94-95	24.08083514314572	26.04391974683814	25.734411517350868	24.140833592665274
96-97	23.699961200549073	26.01532578311695	25.86064236606106	24.42407065027292
98-99	23.5102199178174	26.025886343462133	25.77123344443857	24.6926602942819
100-101	23.53568376398108	26.392964139509147	25.795913898374334	24.27543819813544
102-103	23.510777833705024	26.308017885288727	25.90732569375301	24.273878587253243
104-105	23.659040466220713	26.327971809842087	25.701989234334178	24.310998489603026
106-107	23.6288750700383	26.14893128438766	25.891043364043025	24.331150281531013
108-109	23.532798431595012	26.24216220029349	25.828640879788196	24.396398488323303
110-111	23.40983141567624	26.811204956858226	25.70418602327054	24.07477760419499
112-113	23.343326793495432	26.92682729684251	25.79154082989808	23.93830507976398
114-115	23.437589551942697	26.95577768927699	25.634009384676204	23.972623374104113
116-117	23.306672642972085	26.878347894454528	25.67218740126237	24.142792061311017
118-119	23.280204645289103	26.622101661786274	25.801069883922967	24.296623809001655
120-121	23.238824418146653	26.74563352792132	25.936197480350142	24.07934457358189
122-123	23.25319437142552	26.947088739948644	25.73940160116272	24.06031528746312
124-125	23.18839461569068	26.81279939924516	25.644538447097236	24.35426753796692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	495.0
1	446.0
2	356.0
3	267.5
4	200.0
5	171.5
6	167.0
7	216.0
8	267.5
9	293.0
10	303.0
11	294.5
12	314.5
13	358.5
14	392.5
15	430.0
16	474.0
17	546.0
18	624.5
19	651.0
20	702.5
21	793.5
22	929.0
23	1174.5
24	1455.0
25	1774.5
26	2388.0
27	3340.0
28	4184.0
29	5416.5
30	7199.0
31	9058.5
32	11690.0
33	14737.5
34	17521.0
35	20361.0
36	23940.0
37	27235.5
38	29258.5
39	31557.0
40	33878.5
41	35584.0
42	37567.5
43	40588.0
44	43930.5
45	47111.0
46	49938.5
47	52507.5
48	55893.5
49	59156.5
50	61001.0
51	61300.5
52	59972.0
53	58771.0
54	57943.5
55	55822.5
56	52735.5
57	48202.5
58	42959.5
59	37862.0
60	32196.5
61	26851.0
62	21920.5
63	16652.0
64	12282.0
65	9232.5
66	6846.5
67	5111.0
68	3843.0
69	2923.5
70	2257.5
71	1729.5
72	1287.5
73	957.0
74	722.0
75	529.5
76	369.0
77	240.0
78	150.5
79	101.5
80	74.0
81	51.5
82	42.0
83	29.0
84	20.5
85	20.0
86	15.0
87	9.5
88	10.5
89	8.0
90	5.0
91	3.5
92	3.5
93	3.0
94	3.0
95	3.5
96	2.0
97	0.5
98	1.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016605766609624144
2	0.010507188606974568
3	0.010213281233352902
4	0.011682818101461235
5	0.007421161183947073
6	0.028949876301734127
7	0.012931924439353317
8	0.012123679161893733
9	0.008964174895460822
10-11	0.008155929618001238
12-13	0.007237469075433532
14-15	0.006502700641379366
16-17	0.005069902194973743
18-19	0.0064292237979739495
20-21	0.0057679322073252
22-23	0.006025101159244158
24-25	0.0066496543281901995
26-27	0.005033163773271034
28-29	0.006796608015001032
30-31	0.005914885894136033
32-33	0.008229406461406654
34-35	0.008302883304812072
36-37	0.007751806979271448
38-39	0.007604853292460616
40-41	0.007384422762244365
42-43	0.007825283822676865
44-45	0.007972237509487697
46-47	0.009588728064406863
48-49	0.00856005225673103
50-51	0.00598836273754145
52-53	0.006098578002649575
54-55	0.006392485376271241
56-57	0.00793549908778499
58-59	0.007604853292460616
60-61	0.006025101159244158
62-63	0.006355746954568533
64-65	0.003820795857081662
66-67	0.004261656917514161
68-69	0.007163992232028116
70-71	0.005363809568595409
72-73	0.004812733243054785
74-75	0.004335133760919577
76-77	0.005069902194973743
78-79	0.0033431963749464538
80-81	0.0033064579532437453
82-83	0.0061353164243522825
84-85	0.009294820690785195
86-87	0.007641591714163324
88-89	0.005400547990298117
90-91	0.005657716942217075
92-93	0.00856005225673103
94-95	0.007678330135866032
96-97	0.009478512799298736
98-99	0.007421161183947073
100-101	0.008523313835028322
102-103	0.005731193785622492
104-105	0.005327071146892701
106-107	0.00811919119629853
108-109	0.007347684340541657
110-111	0.008155929618001238
112-113	0.006098578002649575
114-115	0.0022777821455679135
116-117	0.0031595042664329127
118-119	0.15679958382715894
120-121	0.010948049667407068
122-123	0.001800182663432706
124-125	0.0013593216030002063
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1360973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.95971508669929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.2185212856436	54.89069953937473
2	9.502539679766503	12.535696197549065
3	2.8866635689969833	5.712105196865866
4	1.2936198785259971	3.413071944722613
5	0.7663664775200055	2.5274657254608446
6	0.49347485915774436	1.9529676667496325
7	0.34470559038259063	1.5915677771299703
8	0.24888309897393202	1.3133006638572269
9	0.19269533647877796	1.143911654340826
>10	0.9969481239873098	11.536689975298327
>50	0.04354980902636033	1.9135658991724158
>100	0.011586667891235821	1.224479744237578
>500	2.2281182451456698E-4	0.08986973629080763
>1k	2.2281182451456698E-4	0.1546082789500076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.347684340541656E-5	0.0
2	0.0	0.0	0.0	7.347684340541656E-5	0.0
3	0.0	0.0	0.0	7.347684340541656E-5	0.0
4	0.0	0.0	0.0	1.4695368681083313E-4	0.0
5	0.0	0.0	0.0	1.4695368681083313E-4	0.0
6	0.0	0.0	0.0	1.4695368681083313E-4	0.0
7	0.0	0.0	0.0	1.4695368681083313E-4	0.0
8	0.0	0.0	0.0	1.4695368681083313E-4	0.0
9	0.0	0.0	0.0	1.4695368681083313E-4	0.0
10-11	0.0	0.0	0.0	2.204305302162497E-4	0.0
12-13	0.0	0.0	0.0	2.204305302162497E-4	1.4695368681083313E-4
14-15	0.0	0.0	0.0	2.9390737362166626E-4	2.204305302162497E-4
16-17	0.0	0.0	0.0	3.6738421702708283E-4	2.204305302162497E-4
18-19	0.0	0.0	0.0	3.6738421702708283E-4	3.6738421702708283E-4
20-21	0.0	0.0	0.0	4.041226387297911E-4	5.510763255406242E-4
22-23	0.0	0.0	0.0	5.14337903837916E-4	6.612915906487491E-4
24-25	3.673842170270828E-5	0.0	0.0	9.184605425677071E-4	7.347684340541657E-4
26-27	7.347684340541656E-5	0.0	0.0	0.0016164905549191644	8.817221208649988E-4
28-29	7.347684340541656E-5	0.0	0.0	0.005731193785622492	8.817221208649988E-4
30-31	7.347684340541656E-5	0.0	0.0	0.021418499852678928	8.817221208649988E-4
32-33	7.347684340541656E-5	0.0	0.0	0.050515329841223894	8.817221208649988E-4
34-35	7.347684340541656E-5	0.0	0.0	0.08593116836263467	9.551989642704153E-4
36-37	7.347684340541656E-5	0.0	0.0	0.13975295615710231	0.001028675807675832
38-39	7.347684340541656E-5	0.0	0.0	0.21896099334814137	0.001028675807675832
40-41	7.347684340541656E-5	0.0	0.0	0.3095946796887227	0.001028675807675832
42-43	7.347684340541656E-5	0.0	0.0	0.39773015335351986	0.001028675807675832
44-45	7.347684340541656E-5	0.0	0.0	0.49152334396053415	0.0011021526510812485
46-47	7.347684340541656E-5	0.0	0.0	0.5956400310660094	0.0012123679161893733
48-49	7.347684340541656E-5	0.0	0.0	0.7045694514145395	0.0013225831812974983
50-51	7.347684340541656E-5	0.0	0.0	0.8159970844388537	0.0013960600247029148
52-53	7.347684340541656E-5	0.0	0.0	0.9296290227653303	0.0014695368681083313
54-55	7.347684340541656E-5	0.0	0.0	1.0588013134720526	0.0015430137115137479
56-57	7.347684340541656E-5	0.0	0.0	1.1956151958929384	0.0015430137115137479
58-59	7.347684340541656E-5	0.0	0.0	1.3386746100032845	0.0015430137115137479
60-61	7.347684340541656E-5	0.0	0.0	1.4852976510187932	0.0015430137115137479
62-63	7.347684340541656E-5	0.0	0.0	1.6407746516646546	0.0015430137115137479
64-65	7.347684340541656E-5	0.0	0.0	1.7904102432597855	0.0015430137115137479
66-67	7.347684340541656E-5	0.0	0.0	1.9687018037830288	0.0015797521332164561
68-69	7.347684340541656E-5	0.0	0.0	2.1552595091893814	0.0016164905549191644
70-71	7.347684340541656E-5	0.0	0.0	2.356181937481493	0.0018369210851354142
72-73	7.347684340541656E-5	0.0	0.0	2.545862408732576	0.0020940900370543724
74-75	7.347684340541656E-5	0.0	0.0	2.746895052289796	0.0021308284587570805
76-77	7.347684340541656E-5	0.0	0.0	2.986980638116994	0.0021675668804597885
78-79	7.347684340541656E-5	0.0	0.0	3.2509461980509533	0.0022410437238652055
80-81	7.347684340541656E-5	0.0	0.0	3.5003633429906396	0.0022777821455679135
82-83	7.347684340541656E-5	0.0	0.0	3.7531604227269755	0.00235125898897333
84-85	7.347684340541656E-5	0.0	0.0	4.016317737383475	0.00235125898897333
86-87	7.347684340541656E-5	0.0	0.0	4.311364002077925	0.0023879974106760385
88-89	7.347684340541656E-5	0.0	0.0	4.651929171262031	0.0024247358323787466
90-91	7.347684340541656E-5	0.0	0.0	4.994698645748299	0.002498212675784163
92-93	7.347684340541656E-5	0.0	0.0	5.3295693595684845	0.0026451663625949966
94-95	7.347684340541656E-5	0.0	0.0	5.669546714005348	0.0027921200494058296
96-97	7.347684340541656E-5	0.0	0.0	6.056696201908488	0.0027921200494058296
98-99	7.347684340541656E-5	0.0	0.0	6.4865357358301745	0.0027921200494058296
100-101	7.347684340541656E-5	0.0	0.0	6.937573339074325	0.0028288584711085377
102-103	7.347684340541656E-5	0.0	0.0	7.392101092380231	0.0029390737362166627
104-105	7.347684340541656E-5	0.0	0.0	7.8652184870677075	0.003012550579622079
106-107	7.347684340541656E-5	0.0	0.0	8.382385249376732	0.0030492890013247877
108-109	7.347684340541656E-5	0.0	0.0	8.930302070650924	0.0031595042664329122
110-111	7.347684340541656E-5	0.0	0.0	9.509850673011147	0.0032329811098383288
112-113	7.347684340541656E-5	0.0	0.0	10.090207520648828	0.0032329811098383288
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1620	0.0	60.601814	1
CGTAGCG	90	9.728865E-8	46.276047	3
GTATCAA	2675	0.0	44.708385	1
GTACATG	7855	0.0	37.26796	1
TCAACGC	3225	0.0	36.897846	4
CAACGCA	3315	0.0	35.894775	5
TACATGG	8125	0.0	35.80851	2
ACATGGG	8380	0.0	34.64787	3
AACGCAG	3500	0.0	34.336193	6
CATGGGG	7075	0.0	34.058796	4
ATCAACG	3510	0.0	33.901867	3
TATCAAC	3630	0.0	32.781143	2
ATGGGGG	4665	0.0	29.843449	5
TGGGGGG	3365	0.0	26.34329	6
ACGCAGA	4525	0.0	26.031515	7
GTGGTAT	940	0.0	25.319134	1
CGCAGAG	4695	0.0	25.088026	8
ATGGGGA	2440	0.0	24.139605	5
GAGTACA	5540	0.0	23.950346	1
AGTACAT	5495	0.0	23.387661	2
>>END_MODULE