FastQCFastQC Report
Mon 15 Aug 2016
SRR2921303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360973
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27730.20375128676322013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.15893041228591603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.13365437815445275No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.070.830761
GTATCAA38650.051.1671641
GTACATG76250.037.68931
TACATGG78200.036.649412
ACATGGG79550.036.1023523
TCAACGC53150.036.0978434
TATCAAC55700.035.301032
CATGGGG66550.034.73864
CAACGCA54900.034.621595
ATCAACG56600.034.1080743
AACGCAG56000.033.5196236
ATGGGGG46450.029.5033115
GTAAGTA8450.028.2053782
ACGCAGA68400.027.4379167
TGTAAGT8850.027.0040911
CGCAGAG70150.026.4947418
ATGGGGA21750.025.4772365
GAGTACT35950.024.83737412-13
TGGGGGG36400.024.2290786
GTGGTAT10650.024.123021
Produced by FastQC (version 0.11.5)