##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921303_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1360973 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.183953686076066 33.0 30.0 34.0 18.0 34.0 2 29.97043144867679 33.0 31.0 34.0 26.0 34.0 3 30.205477257814813 33.0 31.0 34.0 26.0 34.0 4 32.33561429947545 37.0 35.0 37.0 19.0 37.0 5 31.97219121907635 37.0 33.0 37.0 19.0 37.0 6 32.11977166336143 37.0 35.0 37.0 17.0 37.0 7 32.33331814811903 37.0 35.0 37.0 17.0 37.0 8 32.598847295280656 37.0 35.0 37.0 17.0 37.0 9 34.34586799297267 39.0 37.0 39.0 17.0 39.0 10-11 34.56283592694345 39.0 37.0 39.0 21.0 39.0 12-13 34.57887077847981 39.0 37.0 39.0 24.0 39.0 14-15 35.96986053360354 40.5 38.0 41.0 25.0 41.0 16-17 35.99905729209911 41.0 38.0 41.0 25.0 41.0 18-19 36.04462138484746 41.0 38.0 41.0 25.0 41.0 20-21 36.041502292844896 41.0 38.0 41.0 25.0 41.0 22-23 35.99182202732898 41.0 38.0 41.0 25.0 41.0 24-25 35.93877799192195 41.0 38.0 41.0 25.0 41.0 26-27 35.838080182340136 41.0 38.0 41.0 24.0 41.0 28-29 35.75931925174122 40.0 37.5 41.0 23.5 41.0 30-31 35.650239203863705 40.0 37.0 41.0 23.0 41.0 32-33 35.50748435126928 40.0 36.5 41.0 21.5 41.0 34-35 35.420164837950495 40.0 36.0 41.0 20.5 41.0 36-37 35.33472633182289 40.0 36.0 41.0 18.5 41.0 38-39 35.23852750936278 40.0 36.0 41.0 17.0 41.0 40-41 35.10001263801706 40.0 35.5 41.0 15.5 41.0 42-43 34.947318572815185 40.0 35.0 41.0 11.5 41.0 44-45 34.77180370220423 40.0 35.0 41.0 8.0 41.0 46-47 34.51843534001042 40.0 35.0 41.0 2.0 41.0 48-49 34.274832050305186 40.0 35.0 41.0 2.0 41.0 50-51 34.08468206202474 40.0 35.0 41.0 2.0 41.0 52-53 33.90338897244839 39.0 35.0 41.0 2.0 41.0 54-55 33.7093046665878 39.0 35.0 41.0 2.0 41.0 56-57 33.47605316196574 39.0 34.5 41.0 2.0 41.0 58-59 33.41374185968421 39.0 35.0 41.0 2.0 41.0 60-61 33.18155136068093 38.0 34.0 41.0 2.0 41.0 62-63 32.89734733899938 37.0 34.0 40.5 2.0 41.0 64-65 32.600671357918195 37.0 34.0 40.0 2.0 41.0 66-67 32.28796566867969 36.0 34.0 39.5 2.0 41.0 68-69 31.961847516445953 36.0 34.0 39.0 2.0 41.0 70-71 31.634392085662242 35.0 33.0 39.0 2.0 41.0 72-73 31.292923886072685 35.0 33.0 37.0 2.0 40.0 74-75 30.961258966930277 35.0 33.0 37.0 2.0 39.0 76-77 30.65824487333694 35.0 33.0 36.5 2.0 39.0 78-79 30.397174301033157 35.0 33.0 36.0 2.0 38.0 80-81 30.159692734536247 35.0 32.5 36.0 2.0 37.0 82-83 29.951345103833802 35.0 32.0 35.0 2.0 37.0 84-85 29.772104589877976 35.0 32.0 35.0 2.0 36.0 86-87 29.625576701374676 35.0 32.0 35.0 2.0 36.0 88-89 29.496030046150807 35.0 32.0 35.0 2.0 36.0 90-91 29.38968664330593 35.0 32.0 35.0 2.0 35.5 92-93 29.317179694233463 35.0 32.0 35.0 2.0 35.0 94-95 29.24150773013131 35.0 32.0 35.0 2.0 35.0 96-97 29.1748837045261 35.0 31.5 35.0 2.0 35.0 98-99 29.11779329935274 35.0 31.0 35.0 2.0 35.0 100-101 29.061244418515283 35.0 31.0 35.0 2.0 35.0 102-103 28.399280882133592 34.0 29.5 35.0 2.0 35.0 104-105 28.931305396947625 35.0 31.0 35.0 2.0 35.0 106-107 28.981504776362208 35.0 31.0 35.0 2.0 35.0 108-109 29.045113679698275 35.0 31.5 35.0 2.0 35.0 110-111 29.148372157272775 35.0 32.0 35.0 2.0 35.0 112-113 29.10240798311208 35.0 32.0 35.0 2.0 35.0 114-115 29.033094337654017 35.0 32.0 35.0 2.0 35.0 116-117 28.968111784730482 35.0 32.0 35.0 2.0 35.0 118-119 28.887285052679225 35.0 32.0 35.0 2.0 35.0 120-121 28.78352178919053 35.0 32.0 35.0 2.0 35.0 122-123 28.662320266456426 35.0 32.0 35.0 2.0 35.0 124-125 28.14322694131331 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92969.0 3 5869.0 4 4358.0 5 4033.0 6 4311.0 7 4459.0 8 4714.0 9 4762.0 10 4754.0 11 5017.0 12 5768.0 13 7815.0 14 9767.0 15 8367.0 16 4978.0 17 4210.0 18 3682.0 19 3355.0 20 3175.0 21 3254.0 22 3514.0 23 4491.0 24 5744.0 25 7319.0 26 8602.0 27 9978.0 28 11502.0 29 13913.0 30 16745.0 31 21090.0 32 26728.0 33 36404.0 34 55769.0 35 95833.0 36 209719.0 37 462865.0 38 180729.0 39 411.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20393718203937 18.21462803214628 12.168325591683256 26.41310919413109 2 16.08033370243201 19.800613237735064 37.94807097569166 26.17098208414127 3 19.305526266869364 23.415233072221124 28.634366736151268 28.64487392475824 4 13.435806083694898 15.979032182205133 31.975334226956857 38.60982750714311 5 14.024759620869688 37.82470412186815 32.09483704712925 16.05569921013291 6 33.69663260827855 34.0457439511994 16.622448837067406 15.635174603454653 7 29.722469654075574 31.993600089369444 18.591343510866913 19.69258674568807 8 27.921017185544656 34.552005249648566 18.345919801771387 19.18105776303539 9 27.98153845701651 16.05472329362627 18.060406368512208 37.90333188084501 10-11 26.790470711074178 25.609302681012625 25.363296425047082 22.236930182866118 12-13 26.400358178801948 23.03085285445788 26.218254547352643 24.350534419387532 14-15 23.959145405529718 24.004003018428726 24.09889836168682 27.93795321435473 16-17 23.767444321084987 27.040580525844376 24.834989378922288 24.35698577414835 18-19 23.811309996598023 26.045814281400148 26.83771096120202 23.30516476079981 20-21 24.51830418384494 24.962545179074088 26.929226369663468 23.5899242674175 22-23 24.408088918736816 24.492697503918155 26.650712394735237 24.448501182609796 24-25 24.04922801554476 24.93774674442476 26.588110124153825 24.424915115876654 26-27 23.55961506951277 25.206304607071555 26.83671902381605 24.397361299599623 28-29 23.649624202684404 25.662228420402165 26.700088833503678 23.98805854340975 30-31 24.411836237750492 25.11747110339441 26.723123823911276 23.747568834943824 32-33 24.052497735076305 25.314719689516252 26.576206875522146 24.0565756998853 34-35 24.096069503215713 25.489704792086247 26.826358788895888 23.58786691580215 36-37 24.050256691352438 25.48463488989128 26.802111430572097 23.66299698818419 38-39 23.92145178486274 25.69532973835631 26.887271092078972 23.495947384701974 40-41 23.95708805391437 25.559066932260965 26.730618461938626 23.75322655188604 42-43 23.745070622268038 25.50219585546517 26.519152106617838 24.23358141564895 44-45 23.553589968353524 25.65113341704795 26.693549394440595 24.101727220157933 46-47 23.579159909858607 25.661089529329384 26.25526002352728 24.504490537284724 48-49 23.641909134126834 26.06253026327488 26.052574150993447 24.242986451604846 50-51 23.78496854823718 25.934937724701374 26.18181991854357 24.098273808517874 52-53 23.855175672111056 25.905436772074097 26.09772567126607 24.141661884548775 54-55 23.404946314144365 25.626481936085433 26.41621104900685 24.55236070076335 56-57 23.601864254470883 25.834090757127438 25.903379420458744 24.660665567942935 58-59 23.5898875289958 25.975496942261163 26.16896147094762 24.265654057795416 60-61 24.088244219393037 25.76616141539913 26.256913252503907 23.888681112703924 62-63 23.750948769740475 25.71880558982434 26.424550670733364 24.105694969701823 64-65 23.588491468971096 25.33507277513955 26.342293344541 24.73414241134835 66-67 23.618212852128586 25.765059262748046 26.065248906480875 24.551478978642486 68-69 23.437165909977644 25.679605693867547 26.268081732701532 24.61514666345328 70-71 23.46310323569975 25.542534642494747 26.393727134924795 24.60063498688071 72-73 23.527321996836086 25.960323974097943 25.884201964329932 24.62815206473604 74-75 23.28517905939354 25.97939121496165 26.081156643078153 24.654273082566664 76-77 23.271034767037992 25.346167778493765 26.19295166031949 25.189845794148745 78-79 23.190430669822252 25.49609727746252 26.348575614652166 24.96489643806306 80-81 23.508585401767707 25.81028425986408 25.90154249937361 24.7795878389946 82-83 23.545838161374252 25.726851304177234 26.078511476715555 24.64879905773296 84-85 23.378090527879685 25.764949047482936 25.85448058117244 25.002479843464933 86-87 22.93660491427824 26.12009937008302 25.953821273456562 24.989474442182175 88-89 22.9089408827361 25.790959850048456 26.054888671560718 25.245210595654726 90-91 23.30626691345089 25.38771893343953 26.25357005612896 25.052444096980615 92-93 23.367140078664423 25.58019237735411 26.133730990594216 24.91893655338725 94-95 23.310381616681592 25.447014746067705 25.972998729585377 25.26960490766533 96-97 22.938761024753273 25.409165438071263 26.11222938907979 25.539844148095682 98-99 22.720472779401206 25.48052018666057 26.038650289168118 25.760356744770103 100-101 22.79060642643168 25.716638022943876 26.10749809143899 25.38525745918545 102-103 22.692367886798635 25.558074994874993 26.083250733115204 25.66630638521117 104-105 22.777674501992323 25.599185288760324 26.03016371375479 25.592976495492564 106-107 22.87664781005942 25.37978343435175 26.112384301525456 25.631184454063376 108-109 22.784651546579614 25.383531316978836 26.052208313035326 25.77960882340623 110-111 22.581699910688798 25.851312830577278 25.976958371281107 25.590028887452824 112-113 22.53209210666986 25.990702923769927 25.989968151286714 25.487236818273495 114-115 22.44381221656287 25.94976237571383 25.932899427761924 25.673525979961383 116-117 22.311362206899794 25.7096052221654 26.04080331058482 25.93822926034999 118-119 22.29427034922809 25.486655503084926 26.186889820738546 26.032184326948443 120-121 22.241146591445972 25.626922797145866 26.25386396350258 25.87806664790558 122-123 22.19605592696591 25.707895236685165 26.0540496056625 26.041999230686425 124-125 21.834937884490685 25.572228682063745 25.976314730826672 26.6165187026189 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 83.0 1 75.0 2 77.0 3 82.0 4 74.0 5 82.0 6 202.5 7 519.0 8 759.5 9 884.0 10 853.5 11 677.0 12 673.0 13 764.0 14 846.5 15 937.5 16 1021.5 17 1126.5 18 1188.0 19 1262.0 20 1419.5 21 1487.0 22 1564.0 23 1735.0 24 1967.5 25 2310.0 26 2812.0 27 3565.0 28 4316.0 29 5340.5 30 6837.0 31 8410.5 32 10684.5 33 13402.5 34 15994.5 35 18781.5 36 22147.0 37 25295.0 38 27274.5 39 29505.0 40 31888.0 41 33914.0 42 35983.0 43 39095.5 44 42422.5 45 45667.0 46 48443.0 47 51159.5 48 55088.5 49 58506.5 50 60817.0 51 61503.5 52 60791.0 53 59840.5 54 58852.0 55 57200.5 56 54139.5 57 49398.5 58 44491.5 59 39350.0 60 33219.5 61 27584.5 62 22738.0 63 17597.5 64 12888.5 65 9617.5 66 7209.5 67 5509.5 68 4274.5 69 3191.5 70 2444.5 71 1907.5 72 1473.0 73 1192.0 74 914.5 75 677.0 76 533.5 77 372.0 78 286.0 79 220.5 80 172.0 81 142.5 82 127.5 83 119.5 84 111.5 85 101.0 86 90.0 87 92.0 88 94.5 89 80.0 90 74.5 91 69.5 92 62.5 93 58.5 94 50.5 95 44.5 96 34.5 97 25.0 98 17.0 99 8.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3433572892335116 2 0.0 3 0.0 4 0.01623838239259706 5 0.1756096557389456 6 0.04864167033438577 7 0.024173881480382048 8 0.008817221208649988 9 0.0066129159064874906 10-11 0.00235125898897333 12-13 0.05573218572300846 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 1.4695368681083313E-4 94-95 0.0 96-97 1.1021526510812485E-4 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 1.4695368681083313E-4 110-111 1.1021526510812485E-4 112-113 5.510763255406242E-4 114-115 7.347684340541656E-5 116-117 3.6738421702708283E-4 118-119 0.0 120-121 0.0 122-123 0.001432798446405623 124-125 3.673842170270828E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1360973.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.84091716232223 #Duplication Level Percentage of deduplicated Percentage of total 1 84.88122485027063 57.584201437036576 2 8.627055733697194 11.705347467689768 3 2.5617567499022926 5.213757823804238 4 1.194365820923096 3.24107490875011 5 0.6390443462831898 2.167667727962412 6 0.4362833493509154 1.7758717537569566 7 0.3125853740005839 1.4844254924608988 8 0.23021044793173565 1.249415034243038 9 0.1622270671611456 0.9905069722289187 >10 0.9026727177937794 10.764169584454955 >50 0.03969277298436034 1.8231532179880887 >100 0.012231338844372053 1.286228643575296 >500 2.1647695222612684E-4 0.14274787123985969 >1k 4.329539044522537E-4 0.5714320648089444 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2773 0.20375128676322013 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2163 0.15893041228591603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1819 0.13365437815445275 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 7.347684340541656E-5 6 0.0 0.0 0.0 0.0 2.204305302162497E-4 7 0.0 0.0 0.0 0.0 2.204305302162497E-4 8 0.0 0.0 0.0 0.0 2.204305302162497E-4 9 0.0 0.0 0.0 7.347684340541656E-5 2.204305302162497E-4 10-11 0.0 0.0 0.0 7.347684340541656E-5 2.204305302162497E-4 12-13 0.0 7.347684340541656E-5 0.0 7.347684340541656E-5 2.9390737362166626E-4 14-15 0.0 7.347684340541656E-5 0.0 1.4695368681083313E-4 2.9390737362166626E-4 16-17 0.0 7.347684340541656E-5 0.0 2.204305302162497E-4 2.9390737362166626E-4 18-19 0.0 7.347684340541656E-5 0.0 2.204305302162497E-4 4.408610604324994E-4 20-21 0.0 7.347684340541656E-5 0.0 2.57168951918958E-4 4.408610604324994E-4 22-23 0.0 7.347684340541656E-5 0.0 3.6738421702708283E-4 5.14337903837916E-4 24-25 0.0 7.347684340541656E-5 0.0 8.449836991622905E-4 6.612915906487491E-4 26-27 0.0 7.347684340541656E-5 0.0 0.0015430137115137479 6.612915906487491E-4 28-29 0.0 7.347684340541656E-5 0.0 0.00554750167710895 6.612915906487491E-4 30-31 0.0 7.347684340541656E-5 0.0 0.020206131936489555 6.612915906487491E-4 32-33 0.0 7.347684340541656E-5 0.0 0.047392563996493685 6.612915906487491E-4 34-35 0.0 7.347684340541656E-5 0.0 0.08008975931190405 7.347684340541657E-4 36-37 0.0 7.347684340541656E-5 0.0 0.13012748967099275 7.347684340541657E-4 38-39 0.0 7.347684340541656E-5 0.0 0.20503713152281494 7.347684340541657E-4 40-41 0.0 7.347684340541656E-5 0.0 0.28949876301734123 7.347684340541657E-4 42-43 0.0 7.347684340541656E-5 0.0 0.37278476501738095 7.347684340541657E-4 44-45 0.0 7.347684340541656E-5 0.0 0.46055285446515104 7.347684340541657E-4 46-47 0.0 7.347684340541656E-5 0.0 0.5566605656394359 8.082452774595822E-4 48-49 0.0 7.347684340541656E-5 0.0 0.657764702165289 8.817221208649988E-4 50-51 0.0 7.347684340541656E-5 0.0 0.7604485908243588 8.817221208649988E-4 52-53 0.0 7.347684340541656E-5 0.0 0.8666961063885911 0.0010654142293785402 54-55 0.0 7.347684340541656E-5 0.0 0.9873083448385824 0.0012491063378920815 56-57 0.0 7.347684340541656E-5 0.0 1.115929559219764 0.0012858447595947898 58-59 0.0 7.347684340541656E-5 0.0 1.250833043712109 0.0013225831812974983 60-61 0.0 7.347684340541656E-5 0.0 1.3894471087964273 0.0013225831812974983 62-63 0.0 7.347684340541656E-5 0.0 1.5365477492940713 0.0013960600247029148 64-65 0.0 7.347684340541656E-5 0.0 1.6768150433550115 0.0013960600247029148 66-67 0.0 7.347684340541656E-5 0.0 1.8429094478729557 0.0013960600247029148 68-69 0.0 7.347684340541656E-5 0.0 2.017784335177847 0.0013960600247029148 70-71 0.0 7.347684340541656E-5 0.0 2.2063626537778487 0.0015430137115137479 72-73 0.0 7.347684340541656E-5 0.0 2.3839929227104433 0.0018369210851354142 74-75 0.0 7.347684340541656E-5 0.0 2.5726814565755527 0.0018369210851354142 76-77 0.0 7.347684340541656E-5 0.0 2.797226690022506 0.0018369210851354142 78-79 0.0 7.347684340541656E-5 0.0 3.038634859031002 0.0018369210851354142 80-81 0.0 7.347684340541656E-5 0.0 3.270637992083605 0.0018369210851354142 82-83 0.0 7.347684340541656E-5 0.0 3.5069027820537215 0.0019103979285408307 84-85 0.0 7.347684340541656E-5 0.0 3.753344114835489 0.0019103979285408307 86-87 0.0 7.347684340541656E-5 0.0 4.029212923401126 0.0020206131936489555 88-89 0.0 7.347684340541656E-5 0.0 4.34696353270785 0.002057351615351664 90-91 0.0 7.347684340541656E-5 0.0 4.669379921570817 0.002057351615351664 92-93 0.0 7.347684340541656E-5 0.0 4.983126042911946 0.002057351615351664 94-95 0.0 7.347684340541656E-5 0.0 5.298672346916508 0.002057351615351664 96-97 0.0 7.347684340541656E-5 0.0 5.660472323844779 0.0021308284587570805 98-99 0.0 7.347684340541656E-5 0.0 6.065513423117137 0.0021308284587570805 100-101 0.0 7.347684340541656E-5 0.0 6.489585024831499 0.0021308284587570805 102-103 0.0 7.347684340541656E-5 0.0 6.913877057076077 0.002204305302162497 104-105 0.0 7.347684340541656E-5 0.0 7.35899977442609 0.002204305302162497 106-107 0.0 7.347684340541656E-5 0.0 7.849384227313841 0.002204305302162497 108-109 0.0 7.347684340541656E-5 0.0 8.368130741756083 0.0022410437238652055 110-111 3.673842170270828E-5 7.347684340541656E-5 0.0 8.918729467814572 0.0022777821455679135 112-113 7.347684340541656E-5 7.347684340541656E-5 0.0 9.470026223885412 0.0022777821455679135 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2210 0.0 70.83076 1 GTATCAA 3865 0.0 51.167164 1 GTACATG 7625 0.0 37.6893 1 TACATGG 7820 0.0 36.64941 2 ACATGGG 7955 0.0 36.102352 3 TCAACGC 5315 0.0 36.097843 4 TATCAAC 5570 0.0 35.30103 2 CATGGGG 6655 0.0 34.7386 4 CAACGCA 5490 0.0 34.62159 5 ATCAACG 5660 0.0 34.108074 3 AACGCAG 5600 0.0 33.519623 6 ATGGGGG 4645 0.0 29.503311 5 GTAAGTA 845 0.0 28.205378 2 ACGCAGA 6840 0.0 27.437916 7 TGTAAGT 885 0.0 27.004091 1 CGCAGAG 7015 0.0 26.494741 8 ATGGGGA 2175 0.0 25.477236 5 GAGTACT 3595 0.0 24.837374 12-13 TGGGGGG 3640 0.0 24.229078 6 GTGGTAT 1065 0.0 24.12302 1 >>END_MODULE