FastQCFastQC Report
Mon 15 Aug 2016
SRR2921302_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921302_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949460
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.10321656520548522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.053.672531
GTATCAA19700.047.71721
GTACATG48950.041.9324761
TCAACGC21950.041.7417144
CAACGCA22800.040.1855555
AACGCAG23450.039.071676
TACATGG52500.038.6437452
ACATGGG52400.038.3768653
CATGGGG45950.037.80784
ATCAACG24800.037.1846853
TATCAAC25350.036.377922
ATGGGGG30000.035.300665
ACGCAGA30100.030.0426547
TGGGGGG22400.029.7477476
CGCAGAG31300.028.8908588
GGGTTAG2603.6379788E-1127.4594611
TACCGTA900.009534107526.441057
GAGTACA33750.025.2084621
AGTACAT33300.025.0131232
ATGGGGC11600.024.1059345
Produced by FastQC (version 0.11.5)