##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921302_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	949460
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.084034082531122	31.0	31.0	34.0	26.0	34.0
2	30.142534703936974	33.0	31.0	34.0	26.0	34.0
3	30.141693172961475	33.0	31.0	34.0	26.0	34.0
4	33.473807216733725	37.0	35.0	37.0	28.0	37.0
5	33.56347713437112	37.0	35.0	37.0	28.0	37.0
6	33.61691487793061	37.0	35.0	37.0	28.0	37.0
7	33.51462199566069	37.0	35.0	37.0	28.0	37.0
8	33.539349735639206	37.0	35.0	37.0	28.0	37.0
9	35.05644892886483	39.0	37.0	39.0	27.0	39.0
10-11	35.08149000484486	39.0	37.0	39.0	27.0	39.0
12-13	35.03986107892908	39.0	37.0	39.0	27.0	39.0
14-15	36.327073810376426	40.0	38.0	41.0	27.0	41.0
16-17	36.332296252606746	40.0	38.0	41.0	27.0	41.0
18-19	36.32640922208414	40.0	38.0	41.0	27.0	41.0
20-21	36.34892149221663	40.0	38.0	41.0	27.0	41.0
22-23	36.32629705306174	40.0	38.0	41.0	27.0	41.0
24-25	36.32958576453984	40.5	38.0	41.0	27.0	41.0
26-27	36.311653992795904	40.0	38.0	41.0	27.0	41.0
28-29	36.24301603016451	40.0	37.0	41.0	27.0	41.0
30-31	36.16405377793693	40.0	37.0	41.0	27.0	41.0
32-33	36.06655783287342	40.0	37.0	41.0	27.0	41.0
34-35	35.9506077138584	40.0	37.0	41.0	26.5	41.0
36-37	35.841839045352096	40.0	36.5	41.0	26.0	41.0
38-39	35.73758030880711	40.0	36.0	41.0	25.0	41.0
40-41	35.62273766140754	40.0	36.0	41.0	25.0	41.0
42-43	35.44327038527163	40.0	35.5	41.0	24.0	41.0
44-45	35.227615170728626	40.0	35.0	41.0	23.5	41.0
46-47	35.00598761401217	40.0	35.0	41.0	23.0	41.0
48-49	34.79383175699871	40.0	35.0	41.0	21.5	41.0
50-51	34.61983074589767	39.0	35.0	41.0	20.0	41.0
52-53	34.43231257767573	39.0	35.0	41.0	19.5	41.0
54-55	34.23004444631685	39.0	34.5	41.0	18.0	41.0
56-57	33.994232511111576	38.5	34.0	40.5	16.5	41.0
58-59	33.73864828428791	38.0	34.0	40.0	13.5	41.0
60-61	33.459083057738084	37.5	34.0	40.0	11.5	41.0
62-63	33.132164598824595	37.0	33.0	40.0	8.0	41.0
64-65	32.78995428980684	36.5	33.0	39.5	7.0	41.0
66-67	32.45918890737893	36.0	33.0	39.0	4.0	41.0
68-69	32.100533461125266	35.5	33.0	39.0	2.0	40.5
70-71	32.0330872285299	35.0	33.0	39.0	2.0	40.0
72-73	31.73892633707581	35.0	33.0	37.5	2.0	40.0
74-75	31.41589061150549	35.0	33.0	37.0	2.0	39.0
76-77	31.110285846691802	35.0	32.0	36.5	2.0	39.0
78-79	30.82689160154193	35.0	32.0	36.0	2.0	38.5
80-81	30.554370378952246	35.0	32.0	36.0	2.0	37.0
82-83	30.330675857856043	35.0	32.0	35.0	2.0	37.0
84-85	30.11487424430729	35.0	32.0	35.0	2.0	36.5
86-87	29.942613696206266	35.0	32.0	35.0	2.0	36.0
88-89	29.801168032355235	35.0	31.0	35.0	2.0	36.0
90-91	29.675852589893204	35.0	31.0	35.0	2.0	35.5
92-93	29.568000231710656	35.0	31.0	35.0	2.0	35.0
94-95	29.448381185094686	35.0	31.0	35.0	2.0	35.0
96-97	29.357471615444567	35.0	31.0	35.0	2.0	35.0
98-99	29.281703810587068	35.0	31.0	35.0	2.0	35.0
100-101	29.19702357129316	35.0	31.0	35.0	2.0	35.0
102-103	28.40448886735618	33.5	29.0	35.0	2.0	35.0
104-105	28.968107134581764	34.0	30.5	35.0	2.0	35.0
106-107	29.040550418132412	35.0	31.0	35.0	2.0	35.0
108-109	28.982880268784363	35.0	31.0	35.0	2.0	35.0
110-111	28.908469551113264	35.0	30.0	35.0	2.0	35.0
112-113	28.822250542413585	34.0	30.0	35.0	2.0	35.0
114-115	28.70447412213258	34.0	30.0	35.0	2.0	35.0
116-117	28.576302319213028	34.0	30.0	35.0	2.0	35.0
118-119	28.57397941988077	34.5	30.0	35.0	2.0	35.0
120-121	28.73050418132412	35.0	31.0	35.0	2.0	35.0
122-123	28.602983274703515	35.0	31.0	35.0	2.0	35.0
124-125	28.062090030122384	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43607.0
3	5778.0
4	3471.0
5	3001.0
6	3093.0
7	3384.0
8	3486.0
9	3612.0
10	4156.0
11	4891.0
12	4973.0
13	5045.0
14	5259.0
15	4548.0
16	3379.0
17	2970.0
18	2720.0
19	2664.0
20	2527.0
21	2587.0
22	2995.0
23	4212.0
24	6376.0
25	7834.0
26	7574.0
27	8289.0
28	9681.0
29	11645.0
30	13773.0
31	17297.0
32	22637.0
33	30975.0
34	48251.0
35	78698.0
36	166258.0
37	303273.0
38	94412.0
39	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31974040283699	17.891494621922917	11.375230823844932	25.41353415139516
2	16.383891562673476	20.40689278141363	38.724757489669145	24.48445816624374
3	19.757242640436598	25.57695163918626	28.295989963585345	26.3698157567918
4	13.46965561628915	15.311712922631907	35.43835107060956	35.78028039046938
5	13.5321207218367	39.24197034621365	32.950442194498166	14.275466737451492
6	33.037147853937086	35.28076866347795	17.325480941444194	14.356602541140775
7	28.814798351291255	33.334948475218276	20.250297835674765	17.599955337815704
8	27.604103026498183	35.636984540969166	19.248895031116092	17.51001740141655
9	28.533870660244304	16.222294081153077	17.87630175554645	37.36753350305617
10-11	26.453832991624672	26.279037556002745	26.587970977321884	20.679158475050706
12-13	25.916337074272832	23.694261142505045	27.030508581690636	23.358893201531487
14-15	23.632320932116226	24.671651168436803	24.286780361898632	27.409247537548335
16-17	23.914126519348656	27.7057571991321	24.76996482062733	23.610151460891913
18-19	23.952389403740533	27.126674920911448	26.173241184924752	22.747694490423267
20-21	24.776599038986152	26.09662381216203	25.90497928195708	23.221797866894736
22-23	24.534529157428523	25.57527530110647	26.034610786642297	23.855584754822708
24-25	24.12994603487747	25.51306891283477	26.166738377907535	24.190246674380234
26-27	23.81276137682026	25.841053401578545	26.072984597430327	24.273200624170872
28-29	23.961142101183054	26.51531270012471	25.85379520711854	23.669749991573696
30-31	24.74759470432071	25.939696581738975	25.886927272621946	23.425781441318367
32-33	24.333537849004237	25.826221366708886	26.003755013047748	23.836485771239126
34-35	24.093674244638567	26.158459610921252	26.223290269134132	23.52457587530605
36-37	24.1405116062532	26.322938017825958	26.084946102915307	23.451604273005533
38-39	24.30276870185107	26.32663013825603	25.831421595579513	23.539179564313386
40-41	24.27035419249441	25.997657465407066	26.036313499382242	23.695674842716286
42-43	23.982909187812503	26.090295018216786	25.8029026784313	24.12389311553941
44-45	23.544975774173164	26.270855277017063	26.258795028439014	23.92537392037076
46-47	23.60843539106007	26.029275917676888	26.05576686282957	24.306521828433475
48-49	23.721783167911152	26.1847883217522	25.974969164378354	24.118459345958296
50-51	23.890300580622224	26.272247310836967	25.97585348834147	23.861598620199334
52-53	23.861086944157133	26.318286859052005	25.92657154834456	23.894054648446307
54-55	23.59034747387718	25.927853049711423	26.388876454259204	24.092923022152192
56-57	23.80177206283534	26.203178421785502	25.84842880044491	24.14662071493424
58-59	23.831224197560253	26.399421108982867	25.98252688667545	23.78682780678143
60-61	24.285348646638873	26.271047193321355	26.05649379511994	23.387110364919835
62-63	24.041076015064082	26.28825992550114	26.02478064019726	23.645883419237514
64-65	23.835534485537067	25.982671653534183	26.03249117625684	24.149302684671913
66-67	23.806632333587345	26.286608361170373	25.852713933546056	24.054045371696223
68-69	23.655161953554217	26.166463733189527	26.147030570629276	24.031343742626984
70-71	23.71754259848802	26.044601031685215	26.205173171971236	24.03268319785553
72-73	23.709372649885932	26.713726878037374	25.692895256271708	23.884005215804986
74-75	23.631551146564206	26.58070924092357	25.838522008355596	23.94921760415663
76-77	23.598342357258982	26.064320288007263	25.92660470255277	24.41073265218099
78-79	23.503049197939816	26.109455146772277	26.225472125380488	24.162023529907415
80-81	23.867057076351074	26.42854133532751	25.68672782616939	24.01767376215203
82-83	23.941452306370035	26.47348785672004	25.822086044654913	23.762973792255014
84-85	23.96168473008622	26.58685372079759	25.53554321659303	23.915918332523162
86-87	23.5139252932493	26.839954055458914	25.650525513071077	23.995595138220708
88-89	23.497144301637583	26.330460519213094	25.939325955125113	24.233069224024202
90-91	23.943917132259656	26.13858090732571	25.96368401012622	23.953817950288414
92-93	23.995557168279362	26.33036108804085	25.827040287256775	23.847041456423014
94-95	23.98305883641676	26.283239950137112	25.676174566451458	24.057526646994663
96-97	23.504882082179083	26.313580298928784	25.915008584459493	24.266529034432637
98-99	23.48131037998576	26.29502298283976	25.788178688945905	24.435487948228573
100-101	23.46189212731871	26.55295940475902	25.810488130887286	24.174660337034982
102-103	23.29352991733866	26.563698104940297	25.861950925621645	24.280821052099398
104-105	23.59456950091925	26.563975414347034	25.660946129487037	24.180508955246676
106-107	23.57648476434891	26.422698931906048	25.89647267252053	24.104343631224516
108-109	23.431861606098973	26.488720315649992	25.931213832649046	24.14820424560199
110-111	23.327206656835898	26.958816094375393	25.785759427006532	23.928217821782177
112-113	23.320109393736878	26.962143510553084	25.781731793647563	23.936015302062483
114-115	23.510233185929955	27.111373021126767	25.62454216215846	23.75385163078482
116-117	23.290727135132972	26.939594904951015	25.7071205242295	24.062557435686514
118-119	23.258056009704127	26.72032065819313	25.87004904804599	24.15157428405675
120-121	23.239955127901283	26.828880356870116	26.035244610871477	23.895919904357125
122-123	23.204662545283345	26.983264318857934	25.74168837686696	24.07038475899176
124-125	23.17661321070692	26.833287341406848	25.74664908440958	24.24345036347665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	633.0
1	537.0
2	387.0
3	294.5
4	240.0
5	229.0
6	239.5
7	327.0
8	450.5
9	492.0
10	488.5
11	474.5
12	506.5
13	553.5
14	579.0
15	641.0
16	688.5
17	700.5
18	754.5
19	823.0
20	872.5
21	935.0
22	1058.5
23	1324.5
24	1637.5
25	1975.5
26	2465.5
27	3185.0
28	3847.0
29	4562.5
30	5767.5
31	6957.5
32	8325.0
33	9868.5
34	11451.0
35	13146.5
36	15114.0
37	17127.0
38	18834.0
39	20593.5
40	22403.0
41	24075.0
42	25676.0
43	28049.5
44	30535.5
45	32819.5
46	35141.0
47	37439.5
48	40347.0
49	42509.5
50	42953.0
51	42958.5
52	42197.0
53	41351.5
54	40499.0
55	38389.5
56	35593.5
57	31900.5
58	28625.5
59	25601.5
60	21753.5
61	18263.5
62	15120.0
63	11647.5
64	8452.0
65	6264.0
66	4741.5
67	3679.5
68	2841.5
69	2157.0
70	1685.5
71	1295.0
72	962.5
73	750.5
74	596.0
75	411.5
76	291.5
77	206.0
78	126.5
79	84.0
80	63.5
81	46.5
82	34.0
83	27.0
84	24.5
85	20.5
86	15.5
87	15.0
88	10.5
89	6.0
90	5.5
91	3.5
92	3.0
93	4.0
94	4.0
95	2.5
96	2.0
97	1.0
98	1.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015061192677943251
2	0.012533440060666064
3	0.012322794009226297
4	0.014850546626503485
5	0.01095359467486782
6	0.029490447201567207
7	0.011690855854907
8	0.011796178880626883
9	0.007267288774671919
10-11	0.0067933351589324455
12-13	0.005792766414593559
14-15	0.005318812798854085
16-17	0.004212921028795315
18-19	0.005213489773134203
20-21	0.004844859183114612
22-23	0.00473953615739473
24-25	0.005318812798854085
26-27	0.004265582541655257
28-29	0.00547679733743391
30-31	0.004686874644534788
32-33	0.006582689107492681
34-35	0.0066880121332125635
36-37	0.006108735491753207
38-39	0.006530027594632738
40-41	0.006424704568912856
42-43	0.006424704568912856
44-45	0.006319381543192973
46-47	0.008320519031870748
48-49	0.007477934826111684
50-51	0.005002843721694437
52-53	0.004844859183114612
54-55	0.005318812798854085
56-57	0.006108735491753207
58-59	0.006161397004613148
60-61	0.004844859183114612
62-63	0.005213489773134203
64-65	0.003475659848756135
66-67	0.003738967413055842
68-69	0.00547679733743391
70-71	0.004476228593095022
72-73	0.00400227497735555
74-75	0.003844290438775725
76-77	0.004160259515935374
78-79	0.0031596907715964865
80-81	0.0031596907715964865
82-83	0.004844859183114612
84-85	0.007741242390411391
86-87	0.005740104901733617
88-89	0.004476228593095022
90-91	0.004581551618814905
92-93	0.006898658184652329
94-95	0.0059507509531733825
96-97	0.007372611800391802
98-99	0.0058980894403134415
100-101	0.006635350620352622
102-103	0.004634213131674847
104-105	0.004686874644534788
106-107	0.006161397004613148
108-109	0.005687443388873675
110-111	0.006319381543192973
112-113	0.004792197670254671
114-115	0.0025804141301371307
116-117	0.0030017062330166618
118-119	0.14850546626503486
120-121	0.010005687443388873
122-123	0.0022644450529774817
124-125	0.001895814462957892
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	949460.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.71096660228136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.63048737752597	55.12359683772367
2	9.775644567641402	13.042853965353975
3	3.0494088304280695	6.1028703193016645
4	1.450388557208642	3.8702729040110704
5	0.7893428115075741	2.6328910968116324
6	0.5117885664748824	2.0485145979321198
7	0.36581136419032584	1.708254078946507
8	0.2552963871359489	1.3624855004727503
9	0.19123636054601403	1.1481806215374284
>10	0.9437838501732538	10.62246025831668
>50	0.02875699622797083	1.2445583796184074
>100	0.0072646908796711995	0.7096734909621092
>500	7.896400603176773E-4	0.383387949012018
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	980	0.10321656520548522	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0532302571988288E-4
2	0.0	0.0	0.0	0.0	1.0532302571988288E-4
3	0.0	0.0	0.0	0.0	1.0532302571988288E-4
4	0.0	0.0	0.0	0.0	1.0532302571988288E-4
5	0.0	0.0	0.0	0.0	1.0532302571988288E-4
6	0.0	0.0	0.0	0.0	1.0532302571988288E-4
7	0.0	0.0	0.0	0.0	1.0532302571988288E-4
8	0.0	0.0	0.0	0.0	1.0532302571988288E-4
9	0.0	0.0	0.0	0.0	2.1064605143976577E-4
10-11	0.0	0.0	0.0	0.0	2.1064605143976577E-4
12-13	0.0	0.0	0.0	0.0	2.1064605143976577E-4
14-15	0.0	0.0	0.0	0.0	4.212921028795316E-4
16-17	0.0	0.0	0.0	0.0	5.266151285994145E-4
18-19	0.0	0.0	0.0	0.0	5.266151285994145E-4
20-21	0.0	0.0	0.0	1.0532302571988288E-4	5.266151285994145E-4
22-23	0.0	0.0	0.0	2.1064605143976577E-4	5.266151285994145E-4
24-25	0.0	0.0	0.0	3.1596907715964866E-4	5.266151285994145E-4
26-27	0.0	0.0	0.0	5.792766414593559E-4	5.792766414593559E-4
28-29	0.0	0.0	0.0	0.0024224295915573064	6.319381543192973E-4
30-31	0.0	0.0	0.0	0.011269563752027468	6.319381543192973E-4
32-33	0.0	0.0	0.0	0.027331325174309605	6.319381543192973E-4
34-35	0.0	0.0	0.0	0.04650011585532829	6.319381543192973E-4
36-37	0.0	0.0	0.0	0.07188296505382007	6.319381543192973E-4
38-39	0.0	0.0	0.0	0.11769848124196913	6.845996671792388E-4
40-41	0.0	0.0	0.0	0.1873170012428117	7.372611800391802E-4
42-43	0.0	0.0	0.0	0.24529732690160722	7.372611800391802E-4
44-45	0.0	0.0	0.0	0.30575274366481997	7.372611800391802E-4
46-47	0.0	0.0	0.0	0.3669980831209319	7.372611800391802E-4
48-49	0.0	0.0	0.0	0.4349314347102563	0.001053230257198829
50-51	0.0	0.0	0.0	0.515240241821667	0.001053230257198829
52-53	0.0	0.0	0.0	0.5957070334716575	0.001053230257198829
54-55	0.0	0.0	0.0	0.6874433888736755	0.0011058917700587703
56-57	0.0	0.0	0.0	0.7781791755313547	0.0012112147957786532
58-59	0.0	0.0	0.0	0.8801845259410612	0.0012638763086385946
60-61	0.0	0.0	0.0	0.994617993385714	0.0012638763086385946
62-63	0.0	0.0	0.0	1.1252712067912287	0.0013691993343584775
64-65	0.0	0.0	0.0	1.2423377498788786	0.0013691993343584775
66-67	0.0	0.0	0.0	1.3617740610452258	0.0013691993343584775
68-69	0.0	0.0	0.0	1.5047500684599666	0.0013691993343584775
70-71	0.0	0.0	0.0	1.6667895435300064	0.0013691993343584775
72-73	0.0	0.0	0.0	1.8225096370568532	0.0016851684115181261
74-75	0.0	0.0	0.0	1.9782297305837004	0.0017378299243780676
76-77	0.0	0.0	0.0	2.150538200661429	0.001790491437238009
78-79	0.0	0.0	0.0	2.3517051797864053	0.001790491437238009
80-81	0.0	0.0	0.0	2.5510290059612832	0.0018958144629578919
82-83	0.0	0.0	0.0	2.763202241273987	0.002106460514397658
84-85	0.0	0.0	0.0	2.9755861226381315	0.002106460514397658
86-87	0.0	0.0	0.0	3.2098245318391507	0.002106460514397658
88-89	0.0	0.0	0.0	3.4753438796789755	0.002106460514397658
90-91	0.0	0.0	0.0	3.7492364080635308	0.002106460514397658
92-93	0.0	0.0	0.0	4.0289217028626805	0.002106460514397658
94-95	0.0	0.0	0.0	4.313925810460683	0.002106460514397658
96-97	0.0	0.0	0.0	4.626313904745856	0.002106460514397658
98-99	0.0	0.0	0.0	4.969666968592674	0.0022117835401175407
100-101	0.0	0.0	0.0	5.3357171444821265	0.0022117835401175407
102-103	0.0	0.0	0.0	5.7290459840330294	0.0022117835401175407
104-105	1.0532302571988288E-4	0.0	0.0	6.138120615929054	0.0022117835401175407
106-107	1.0532302571988288E-4	0.0	0.0	6.560149979988625	0.0023171065658374236
108-109	1.0532302571988288E-4	0.0	0.0	6.996713921597539	0.0023171065658374236
110-111	1.0532302571988288E-4	0.0	0.0	7.467191877488256	0.0023171065658374236
112-113	1.0532302571988288E-4	0.0	0.0	7.966528342426221	0.0023171065658374236
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1175	0.0	53.67253	1
GTATCAA	1970	0.0	47.7172	1
GTACATG	4895	0.0	41.932476	1
TCAACGC	2195	0.0	41.741714	4
CAACGCA	2280	0.0	40.185555	5
AACGCAG	2345	0.0	39.07167	6
TACATGG	5250	0.0	38.643745	2
ACATGGG	5240	0.0	38.376865	3
CATGGGG	4595	0.0	37.8078	4
ATCAACG	2480	0.0	37.184685	3
TATCAAC	2535	0.0	36.37792	2
ATGGGGG	3000	0.0	35.30066	5
ACGCAGA	3010	0.0	30.042654	7
TGGGGGG	2240	0.0	29.747747	6
CGCAGAG	3130	0.0	28.890858	8
GGGTTAG	260	3.6379788E-11	27.459461	1
TACCGTA	90	0.0095341075	26.44105	7
GAGTACA	3375	0.0	25.208462	1
AGTACAT	3330	0.0	25.013123	2
ATGGGGC	1160	0.0	24.105934	5
>>END_MODULE