FastQCFastQC Report
Mon 15 Aug 2016
SRR2921302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949460
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.22602321319486868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.21348977313420262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.1739936384892465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.070.2903061
GTATCAA23400.054.3848721
TCAACGC31050.040.1162954
GTACATG44050.039.062581
TACATGG45800.037.8672372
ATCAACG33200.037.3388863
CAACGCA32650.037.237725
TATCAAC33950.037.2162132
ACATGGG47000.036.9004143
AACGCAG33050.036.604786
CATGGGG42900.034.870054
ATGGGGG29200.031.8403535
ACCGTAT1403.0615356E-629.7898738
ACGCAGA41000.028.4835267
GTGGTAT6600.028.0628721
CGCAGAG43250.027.1380588
GTTAGGC2109.65174E-825.5422613
TATACCG1401.04391656E-425.542265
TGGGGGG23300.025.3216786
TACCGTA1451.3272327E-424.6549877
Produced by FastQC (version 0.11.5)