FastQCFastQC Report
Mon 15 Aug 2016
SRR2921301_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921301_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135773
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.059.506651
GTATCAA21500.054.2479251
TCAACGC25950.043.3362854
GTACATG70300.041.6461941
CAACGCA27200.041.1259735
TACATGG72800.039.4768942
AACGCAG28850.038.9801256
ATCAACG28950.038.2288863
ACATGGG75250.037.7171753
TATCAAC30750.036.95862
CATGGGG64600.034.171864
ATGGGGG38800.032.0510185
ACGCAGA36450.031.0103637
AGTACAT48000.028.387132
GAGTACA48650.028.3772721
CGCAGAG40450.027.9425958
TGGGGGG30300.027.0996536
TATCTAA3350.026.6424222
GTGGTAT7050.026.1660421
GTGTAGC5900.025.2146821
Produced by FastQC (version 0.11.5)