##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921301_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1135773
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.03035553759422	31.0	31.0	34.0	26.0	34.0
2	30.100609012540357	31.0	31.0	34.0	26.0	34.0
3	30.34205250521011	33.0	31.0	34.0	26.0	34.0
4	33.64144683840873	37.0	35.0	37.0	28.0	37.0
5	33.76759792669838	37.0	35.0	37.0	28.0	37.0
6	33.81121315615004	37.0	35.0	37.0	29.0	37.0
7	33.73651513110454	37.0	35.0	37.0	29.0	37.0
8	33.74755078699705	37.0	35.0	37.0	29.0	37.0
9	35.28500941649432	39.0	37.0	39.0	28.0	39.0
10-11	35.28707408962883	39.0	37.0	39.0	28.0	39.0
12-13	35.20200163236844	39.0	37.0	39.0	27.5	39.0
14-15	36.477628452164296	40.0	38.0	41.0	27.0	41.0
16-17	36.46250571196885	40.0	38.0	41.0	27.0	41.0
18-19	36.46488118664557	40.0	38.0	41.0	27.0	41.0
20-21	36.50662060112364	40.0	38.0	41.0	27.0	41.0
22-23	36.4699491007446	40.0	38.0	41.0	27.0	41.0
24-25	36.47272870547196	40.0	38.0	41.0	27.5	41.0
26-27	36.44899817128951	40.0	38.0	41.0	28.0	41.0
28-29	36.37722414602214	40.0	37.0	41.0	28.5	41.0
30-31	36.295795462649664	40.0	37.0	41.0	28.0	41.0
32-33	36.200208140182944	40.0	37.0	41.0	28.0	41.0
34-35	36.08049451783059	40.0	37.0	41.0	27.0	41.0
36-37	35.96755953874586	40.0	36.5	41.0	27.0	41.0
38-39	35.85976026899741	40.0	36.0	41.0	26.5	41.0
40-41	35.74556799642182	40.0	36.0	41.0	26.0	41.0
42-43	35.54930958915206	40.0	35.5	41.0	25.0	41.0
44-45	35.31790727548551	40.0	35.0	41.0	24.0	41.0
46-47	35.090941587799676	40.0	35.0	41.0	24.0	41.0
48-49	34.877401118005096	39.5	35.0	41.0	23.0	41.0
50-51	34.694309074084345	39.0	35.0	41.0	23.0	41.0
52-53	34.50036891174557	39.0	35.0	41.0	22.0	41.0
54-55	34.293003971744355	39.0	34.0	40.5	21.5	41.0
56-57	34.04663960139922	38.0	34.0	40.0	20.0	41.0
58-59	33.77715969652387	38.0	34.0	40.0	19.5	41.0
60-61	33.483843162322046	37.0	34.0	40.0	18.0	41.0
62-63	33.14640645621968	37.0	33.0	40.0	15.5	41.0
64-65	32.80331589146775	36.0	33.0	39.0	12.5	41.0
66-67	32.4612783540373	36.0	33.0	39.0	9.5	41.0
68-69	32.10239149900552	35.0	32.5	38.5	8.0	40.0
70-71	32.05307046390432	35.0	33.0	38.0	7.5	40.0
72-73	31.760686774557946	35.0	33.0	37.0	6.5	39.5
74-75	31.438750084744044	35.0	32.5	37.0	2.0	39.0
76-77	31.13469681001397	35.0	32.0	36.0	2.0	39.0
78-79	30.850770356400442	35.0	32.0	36.0	2.0	37.5
80-81	30.576554029722487	35.0	32.0	35.5	2.0	37.0
82-83	30.35534257285567	35.0	32.0	35.0	2.0	37.0
84-85	30.137960226207174	35.0	32.0	35.0	2.0	36.0
86-87	29.968987641016295	35.0	31.0	35.0	2.0	36.0
88-89	29.833506783485785	35.0	31.0	35.0	2.0	36.0
90-91	29.705210460188788	35.0	31.0	35.0	2.0	35.0
92-93	29.589606814037666	35.0	31.0	35.0	2.0	35.0
94-95	29.46091780663918	35.0	31.0	35.0	2.0	35.0
96-97	29.365509657299476	35.0	31.0	35.0	2.0	35.0
98-99	29.280847933521926	35.0	31.0	35.0	2.0	35.0
100-101	29.184815099496113	34.0	31.0	35.0	2.0	35.0
102-103	28.35461135279673	33.5	29.0	34.5	2.0	35.0
104-105	28.936005258092948	34.0	30.0	35.0	2.0	35.0
106-107	28.997699364221546	34.0	30.0	35.0	2.0	35.0
108-109	28.938802031744018	34.0	30.0	35.0	2.0	35.0
110-111	28.856346734778867	34.0	30.0	35.0	2.0	35.0
112-113	28.75964387249917	34.0	30.0	35.0	2.0	35.0
114-115	28.6220569603257	34.0	30.0	35.0	2.0	35.0
116-117	28.483052511373312	34.0	29.5	35.0	2.0	35.0
118-119	28.482518513822747	34.0	29.5	35.0	2.0	35.0
120-121	28.654016691715686	35.0	30.5	35.0	2.0	35.0
122-123	28.513585020950487	35.0	30.5	35.0	2.0	35.0
124-125	27.92660461201314	34.5	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46393.0
3	6693.0
4	4292.0
5	3504.0
6	3820.0
7	4276.0
8	4191.0
9	4486.0
10	5171.0
11	5476.0
12	5757.0
13	5826.0
14	6057.0
15	5298.0
16	4313.0
17	3883.0
18	3551.0
19	3262.0
20	3246.0
21	3368.0
22	3752.0
23	4981.0
24	7256.0
25	9268.0
26	9567.0
27	10447.0
28	12206.0
29	14662.0
30	18058.0
31	22796.0
32	29239.0
33	39839.0
34	63285.0
35	103737.0
36	211273.0
37	350949.0
38	91528.0
39	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.89894151006534	16.89894151006534	11.177195794219694	25.024921185649625
2	16.811371893607316	19.300448556851542	38.281166071702174	25.60701347783897
3	20.618381496985847	23.74973803364088	27.81343064099426	27.818449828379016
4	13.632252957187378	14.474169684833788	34.33991738455456	37.55365997342428
5	14.145192464327069	38.72788190179322	32.23494604482836	14.891979589051349
6	34.33868109646759	33.51714067294447	16.929915415156223	15.214262815431715
7	30.523008536291123	32.54199020448706	18.530990065356253	18.404011193865564
8	28.477270806035758	34.86596009975062	18.57327021429477	18.083498879918846
9	28.805727015770426	16.09323130839064	17.574383403629575	37.52665827220936
10-11	27.116833761563537	25.691038512541365	25.81158281983865	21.380544906056446
12-13	26.30312390485465	23.10979013713201	26.254695366885798	24.332390591127538
14-15	24.04277350680592	24.33210971530409	23.489591909152306	28.135524868737686
16-17	24.55046217296134	27.394996310716884	24.189854559478462	23.86468695684331
18-19	24.37364731241118	26.788717462855573	25.683718088019567	23.15391713671368
20-21	25.241555836632664	25.662701894540906	25.494525233323106	23.601217035503325
22-23	25.10618926695947	25.015893169723203	25.536537560711025	24.341380002606304
24-25	24.483404126427306	25.114862675485867	25.70308812859689	24.698645069489938
26-27	24.145828197090832	25.444035501708168	25.68000915718663	24.730127144014368
28-29	24.285618687280035	26.313173426503234	25.287333043938425	24.113874842278307
30-31	25.174835642389187	25.684561060729	25.432780125551474	23.707823171330343
32-33	24.645794819211282	25.429292584640077	25.51703764232156	24.40787495382708
34-35	24.597400859218638	25.812792830393693	25.66380759419662	23.92599871619105
36-37	24.528017793603706	25.892123635960196	25.61158908820989	23.96826948222621
38-39	24.763613390068173	26.001272351450766	25.402386649555226	23.832727608925833
40-41	24.696693333262893	25.592535994621773	25.673588006591586	24.037182665523748
42-43	24.39292305633237	25.67888363705525	25.27393086833708	24.6542624382753
44-45	23.882649159518653	25.874436520225398	25.821649158197868	24.42126516205808
46-47	23.89701576779854	25.5994484308368	25.62172604869905	24.88180975266561
48-49	24.02426041911565	25.872365117898838	25.522045440737113	24.5813290222484
50-51	24.067068325251	25.92194733238972	25.650486590957616	24.36049775140166
52-53	24.399869332955305	25.825319997781126	25.529470085259366	24.245340584004204
54-55	23.862805378706796	25.42127083680953	26.043838142186253	24.67208564229742
56-57	24.23269842988964	25.727743928881658	25.324022648895887	24.71553499233282
58-59	24.082311012884208	26.030109382601363	25.683360592766086	24.204219011748336
60-61	24.76895591671847	25.873591414739977	25.54859762228871	23.80885504625284
62-63	24.504416850289356	25.90777114706031	25.58673778239556	24.001074220254775
64-65	24.244576096656225	25.387578897041703	25.632882275069967	24.734962731232113
66-67	24.220610726678636	25.731094301545525	25.503487187524488	24.54480778425135
68-69	24.024628091168843	25.588296153456696	25.708707003200683	24.67836875217378
70-71	24.083948730185426	25.484869910373796	25.941277624619513	24.489903734821265
72-73	24.15767238813724	26.21257666929055	25.201457751104584	24.42829319146763
74-75	23.95401021633213	26.08528167553834	25.39167522576879	24.569032882360737
76-77	23.82015477392775	25.484251854108535	25.659514968905217	25.036078403058497
78-79	23.869107366122527	25.52181837392361	25.939871801644742	24.66920245830912
80-81	24.214167351977306	25.782883024077734	25.406563837786532	24.59638578615843
82-83	24.39781088578471	25.907218432866475	25.45904083831223	24.235929843036583
84-85	24.330757021751527	26.069073914680136	25.167401550189645	24.432767513378696
86-87	23.77062352099499	26.272235980408343	25.313697650101812	24.643442848494853
88-89	23.734368919045046	25.7097272386279	25.64690354471797	24.90900029760909
90-91	24.381468942472363	25.332147589844283	25.76548693994532	24.520896527738035
92-93	24.340000105663382	25.742945648517807	25.5966018656631	24.320452380155714
94-95	24.24161843247044	25.623243636145908	25.466290801683378	24.668847129700275
96-97	23.87549315841078	25.56428417019287	25.556579429928465	25.00364324146788
98-99	23.668657809836436	25.63564545441738	25.50171876981164	25.193977965934543
100-101	23.639597596143265	25.874656041561185	25.62123847051313	24.86450789178242
102-103	23.632715936601745	25.75751105366293	25.658806235221242	24.95096677451408
104-105	23.808528004775837	25.861460205315232	25.47646112543882	24.85355066447011
106-107	23.770390759417655	25.70375966596401	25.718816644741594	24.807032929876744
108-109	23.73454962500853	25.775330910435702	25.630925624673935	24.859193839881836
110-111	23.60974445740289	26.251946518456098	25.53977501614669	24.598534007994317
112-113	23.539231979924498	26.33016718579746	25.527675357980122	24.60292547629792
114-115	23.54144581368402	26.529124418444937	25.427867801660227	24.501561966210815
116-117	23.498418878541784	26.366977784175933	25.40267639420662	24.731926943075667
118-119	23.400920972157856	25.98185659273814	25.674160350398022	24.94306208470598
120-121	23.369675046702103	26.061072217763222	25.871225913140034	24.698026822394645
122-123	23.471248873547168	26.256751563166542	25.49461569853723	24.77738386474906
124-125	23.216887025818405	26.10042002730332	25.52177639720381	25.160916549674468
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	565.0
1	467.5
2	320.0
3	248.0
4	214.5
5	181.5
6	168.0
7	195.0
8	254.0
9	307.0
10	321.5
11	343.0
12	365.5
13	378.0
14	432.5
15	463.0
16	487.5
17	555.0
18	629.0
19	680.5
20	710.0
21	767.0
22	889.0
23	1094.0
24	1281.0
25	1572.5
26	2099.0
27	2745.0
28	3439.5
29	4303.5
30	5504.5
31	6797.5
32	8638.0
33	10747.0
34	12844.5
35	14904.5
36	17104.0
37	19384.5
38	20949.0
39	22814.0
40	24490.0
41	25574.0
42	27136.0
43	30247.0
44	33792.5
45	36947.0
46	39603.5
47	42391.5
48	46762.5
49	50726.0
50	52190.0
51	52671.0
52	52607.5
53	51662.0
54	50961.5
55	49763.5
56	46994.5
57	42871.5
58	38573.5
59	34081.0
60	29359.5
61	24882.0
62	20568.5
63	16074.5
64	11911.0
65	8994.0
66	6787.5
67	4947.0
68	3772.0
69	2950.0
70	2329.0
71	1770.0
72	1357.5
73	1159.5
74	908.0
75	676.5
76	489.5
77	313.0
78	196.5
79	117.5
80	66.5
81	46.0
82	37.5
83	29.0
84	23.0
85	14.5
86	9.5
87	9.5
88	9.5
89	7.5
90	5.0
91	4.0
92	3.0
93	2.5
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016992832194461394
2	0.011533994909194002
3	0.011533994909194002
4	0.013206864399840462
5	0.009508942367885131
6	0.03037578811963306
7	0.013294910162506066
8	0.012414452535850032
9	0.009068713554557116
10-11	0.008012164402569879
12-13	0.006955615250582643
14-15	0.006163203386592215
16-17	0.004490333895945757
18-19	0.006251249149257817
20-21	0.005502860166600192
22-23	0.0057229745732642
24-25	0.006075157623926612
26-27	0.004666425421276963
28-29	0.006163203386592215
30-31	0.005590905929265795
32-33	0.008496416097230697
34-35	0.007924118639904276
36-37	0.007395844063910658
38-39	0.007087683894581047
40-41	0.0072637754199122535
42-43	0.007483889826576261
44-45	0.00743986694524346
46-47	0.009156759317222719
48-49	0.008452393215897896
50-51	0.005766997454597001
52-53	0.005458837285267391
54-55	0.006383317793256222
56-57	0.007792049995905871
58-59	0.007087683894581047
60-61	0.005194699997270582
62-63	0.005766997454597001
64-65	0.003213670337294512
66-67	0.003874013557286535
68-69	0.0068675694879170406
70-71	0.004930562709273772
72-73	0.004490333895945757
74-75	0.0037419449132881305
76-77	0.0045783796586113595
78-79	0.002905510167964901
80-81	0.0030816016932961074
82-83	0.005502860166600192
84-85	0.009288827961221125
86-87	0.007527912707909062
88-89	0.004842516946608169
90-91	0.005106654234604978
92-93	0.008012164402569879
94-95	0.007175729657246651
96-97	0.009552965249217933
98-99	0.006779523725251437
100-101	0.008628484741229101
102-103	0.005590905929265795
104-105	0.004930562709273772
106-107	0.007659981351907467
108-109	0.007087683894581047
110-111	0.00805618728390268
112-113	0.005326768641268986
114-115	0.002201144066640077
116-117	0.0025093042359696873
118-119	0.16398523296468573
120-121	0.010345377113208362
122-123	0.0015408008466480537
124-125	0.0011886177959856414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1135773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.02236015038389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.2234126873888	54.11382712703422
2	9.449642981502695	12.288761784716312
3	2.8525192210929156	5.564325963893882
4	1.3294610995235496	3.457787936765824
5	0.7501190225037505	2.438725461844639
6	0.495341208291855	1.932495266572759
7	0.3487772162922934	1.587482243900409
8	0.2611603427000713	1.3585009488033373
9	0.18997221011358859	1.1117197318113141
>10	1.0320016412560236	12.018290530484496
>50	0.051322944397114295	2.238012935164736
>100	0.015591534430975778	1.5823171892035897
>500	5.423124050998505E-4	0.214307433630718
>1k	1.3557810127496261E-4	0.09344544617382192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.804576266560308E-5	0.0
8	0.0	0.0	0.0	8.804576266560308E-5	0.0
9	0.0	0.0	0.0	8.804576266560308E-5	1.7609152533120615E-4
10-11	0.0	0.0	0.0	1.3206864399840462E-4	1.7609152533120615E-4
12-13	0.0	0.0	0.0	1.7609152533120615E-4	1.7609152533120615E-4
14-15	0.0	0.0	0.0	1.7609152533120615E-4	2.6413728799680924E-4
16-17	0.0	0.0	0.0	2.2011440666400769E-4	3.521830506624123E-4
18-19	0.0	0.0	0.0	2.6413728799680924E-4	3.521830506624123E-4
20-21	0.0	0.0	0.0	4.4022881332801537E-4	3.521830506624123E-4
22-23	0.0	0.0	0.0	5.282745759936185E-4	4.4022881332801537E-4
24-25	0.0	0.0	0.0	0.001056549151987237	6.163203386592215E-4
26-27	0.0	0.0	0.0	0.0014967779653152521	6.163203386592215E-4
28-29	0.0	0.0	0.0	0.004666425421276963	6.163203386592215E-4
30-31	0.0	0.0	0.0	0.018797770329106255	6.163203386592215E-4
32-33	0.0	0.0	0.0	0.041073348283503834	6.163203386592215E-4
34-35	0.0	0.0	0.0	0.06946810674316083	9.685033893216339E-4
36-37	0.0	0.0	0.0	0.10917674570534781	9.685033893216339E-4
38-39	0.0	0.0	0.0	0.1807579507524831	9.685033893216339E-4
40-41	0.0	0.0	0.0	0.26770314138476614	9.685033893216339E-4
42-43	0.0	0.0	0.0	0.34307031422652234	9.685033893216339E-4
44-45	0.0	0.0	0.0	0.41742496079762414	9.685033893216339E-4
46-47	0.0	0.0	0.0	0.5012885497366111	0.0010125262706544354
48-49	4.402288133280154E-5	0.0	0.0	0.5976546369741137	0.0011445949146528399
50-51	8.804576266560308E-5	0.0	0.0	0.703837826748831	0.001232640677318443
52-53	8.804576266560308E-5	0.0	0.0	0.8111656114382011	0.0012766635586512446
54-55	8.804576266560308E-5	0.0	0.0	0.9239962562941715	0.0013206864399840461
56-57	8.804576266560308E-5	0.0	0.0	1.041009074876758	0.0014087322026496492
58-59	8.804576266560308E-5	0.0	0.0	1.1806056315830715	0.0014967779653152523
60-61	8.804576266560308E-5	0.0	0.0	1.32671757472664	0.0014967779653152523
62-63	8.804576266560308E-5	0.0	0.0	1.4951931415872712	0.0014967779653152523
64-65	8.804576266560308E-5	0.0	0.0	1.6520466677760433	0.0014967779653152523
66-67	8.804576266560308E-5	0.0	0.0	1.8150633973514074	0.0014967779653152523
68-69	8.804576266560308E-5	0.0	0.0	2.0077955718264127	0.0014967779653152523
70-71	8.804576266560308E-5	4.402288133280154E-5	0.0	2.209156231042647	0.0014967779653152523
72-73	8.804576266560308E-5	8.804576266560308E-5	0.0	2.3920713029804372	0.0016728694906464584
74-75	8.804576266560308E-5	8.804576266560308E-5	0.0	2.584187157116783	0.00171689237197926
76-77	8.804576266560308E-5	8.804576266560308E-5	0.0	2.8224830137712376	0.0017609152533120615
78-79	8.804576266560308E-5	8.804576266560308E-5	0.0	3.0830984712614224	0.0017609152533120615
80-81	8.804576266560308E-5	8.804576266560308E-5	0.0	3.3389594575676655	0.0018489610159776646
82-83	8.804576266560308E-5	8.804576266560308E-5	0.0	3.6074990336977546	0.002025052541308871
84-85	8.804576266560308E-5	8.804576266560308E-5	0.0	3.8843589343997436	0.002025052541308871
86-87	8.804576266560308E-5	8.804576266560308E-5	0.0	4.182834069836138	0.002025052541308871
88-89	8.804576266560308E-5	8.804576266560308E-5	0.0	4.515030732373459	0.002025052541308871
90-91	1.7609152533120615E-4	1.7609152533120615E-4	0.0	4.883326157603676	0.002025052541308871
92-93	1.7609152533120615E-4	2.6413728799680924E-4	0.0	5.232999904030119	0.002025052541308871
94-95	1.7609152533120615E-4	2.6413728799680924E-4	0.0	5.578447453848613	0.002025052541308871
96-97	1.7609152533120615E-4	3.081601693296108E-4	0.0	5.97077937228654	0.002113098303974474
98-99	1.7609152533120615E-4	3.521830506624123E-4	0.0	6.423114477981075	0.0022451669479728784
100-101	1.7609152533120615E-4	3.521830506624123E-4	0.0	6.8989578023073275	0.002377235591971283
102-103	2.6413728799680924E-4	3.521830506624123E-4	0.0	7.393554874081353	0.002553327117302489
104-105	2.6413728799680924E-4	3.521830506624123E-4	0.0	7.907654082285809	0.002553327117302489
106-107	2.6413728799680924E-4	3.521830506624123E-4	0.0	8.454110108269873	0.0025973499986352904
108-109	2.6413728799680924E-4	3.521830506624123E-4	0.0	9.027420091866949	0.0026413728799680922
110-111	2.6413728799680924E-4	3.521830506624123E-4	0.0	9.630445520363665	0.002685395761300894
112-113	2.6413728799680924E-4	3.521830506624123E-4	0.0	10.223037525984505	0.0027294186426336953
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1190	0.0	59.50665	1
GTATCAA	2150	0.0	54.247925	1
TCAACGC	2595	0.0	43.336285	4
GTACATG	7030	0.0	41.646194	1
CAACGCA	2720	0.0	41.125973	5
TACATGG	7280	0.0	39.476894	2
AACGCAG	2885	0.0	38.980125	6
ATCAACG	2895	0.0	38.228886	3
ACATGGG	7525	0.0	37.717175	3
TATCAAC	3075	0.0	36.9586	2
CATGGGG	6460	0.0	34.17186	4
ATGGGGG	3880	0.0	32.051018	5
ACGCAGA	3645	0.0	31.010363	7
AGTACAT	4800	0.0	28.38713	2
GAGTACA	4865	0.0	28.377272	1
CGCAGAG	4045	0.0	27.942595	8
TGGGGGG	3030	0.0	27.099653	6
TATCTAA	335	0.0	26.642422	2
GTGGTAT	705	0.0	26.166042	1
GTGTAGC	590	0.0	25.214682	1
>>END_MODULE