FastQCFastQC Report
Mon 15 Aug 2016
SRR2921301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135773
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25730.22654174733859672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.17265774058724762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.1459798744995699No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.076.608681
GTATCAA29250.053.1285061
GTACATG65350.045.9132771
TACATGG66850.044.2214242
ACATGGG68750.042.739233
CATGGGG59550.039.533734
TCAACGC39950.037.893914
ATGGGGG35550.037.2190975
CAACGCA41500.036.6222085
ATCAACG41750.036.5456733
TATCAAC43900.035.4346772
AACGCAG43150.035.3646286
TGGGGGG27500.029.262476
ACGCAGA54350.027.6297427
CATGGGT10600.027.5513364
GAGTACA42200.027.1937391
CGCAGAG56950.026.6798888
AGTACAT42050.026.6467532
TGGTATC6900.025.9134122
GTGGTAT7100.025.254751
Produced by FastQC (version 0.11.5)