FastQCFastQC Report
Mon 15 Aug 2016
SRR2921300_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921300_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974642
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.058.4958651
GTATCAA13100.044.060521
GTACATG43250.040.5868261
TACATGG44650.039.4474952
ACATGGG45350.038.1825453
TCAACGC14850.037.2653924
CAACGCA15250.036.287945
AACGCAG16300.033.9468846
CATGGGG43100.033.6869544
ATCAACG17150.032.961633
ATGGGGG26450.032.39565
TATCAAC17450.031.7129552
TGGGGGG17500.029.919156
CCGTATA1004.4428903E-429.7445749
GAGTACA29800.028.354451
ACGCAGA22650.028.1102521
AGTACAT28200.027.8531282
GTATAGG2803.6379788E-1227.6270481
CGCAGAG23750.026.8083042
AGAGTAC24300.026.4464055
Produced by FastQC (version 0.11.5)