##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921300_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	974642
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.145491370164635	31.0	31.0	34.0	26.0	34.0
2	30.309042704911136	31.0	31.0	34.0	26.0	34.0
3	30.379808175719905	31.0	31.0	34.0	26.0	34.0
4	33.8288109890606	37.0	35.0	37.0	30.0	37.0
5	33.884839766806685	37.0	35.0	37.0	30.0	37.0
6	34.13672815249086	37.0	35.0	37.0	30.0	37.0
7	34.15552171976993	37.0	35.0	37.0	30.0	37.0
8	34.149682652707355	37.0	35.0	37.0	30.0	37.0
9	35.764156480020354	39.0	37.0	39.0	30.0	39.0
10-11	35.76837803008695	39.0	37.0	39.0	30.0	39.0
12-13	35.69981644542304	39.0	37.0	39.0	30.0	39.0
14-15	37.0289829496369	41.0	38.0	41.0	30.0	41.0
16-17	37.01021605881955	41.0	38.0	41.0	30.0	41.0
18-19	37.005795974316726	41.0	38.0	41.0	30.0	41.0
20-21	37.045577247851	41.0	38.0	41.0	30.0	41.0
22-23	37.0055676853655	41.0	38.0	41.0	30.0	41.0
24-25	37.00305394185763	41.0	38.0	41.0	30.0	41.0
26-27	36.98309994849391	41.0	38.0	41.0	30.0	41.0
28-29	36.92556446366974	41.0	38.0	41.0	30.0	41.0
30-31	36.85193948136855	40.0	38.0	41.0	30.0	41.0
32-33	36.781659316959455	40.0	38.0	41.0	30.0	41.0
34-35	36.67151990166646	40.0	38.0	41.0	30.0	41.0
36-37	36.57043458008171	40.0	38.0	41.0	30.0	41.0
38-39	36.479487852975765	40.0	37.0	41.0	29.0	41.0
40-41	36.37835584758301	40.0	37.0	41.0	29.0	41.0
42-43	36.19191713470177	40.0	37.0	41.0	28.0	41.0
44-45	35.95887310417569	40.0	36.0	41.0	27.5	41.0
46-47	35.75811990453931	40.0	36.0	41.0	27.0	41.0
48-49	35.56328323630625	40.0	35.0	41.0	26.5	41.0
50-51	35.38885354827721	40.0	35.0	41.0	26.0	41.0
52-53	35.2111544546613	39.0	35.0	41.0	26.0	41.0
54-55	35.01613310323175	39.0	35.0	41.0	26.0	41.0
56-57	34.77936206319859	39.0	35.0	41.0	25.0	41.0
58-59	34.51788913262511	38.0	34.5	40.0	24.0	41.0
60-61	34.232521274478216	38.0	34.0	40.0	23.5	41.0
62-63	33.91192047951966	37.0	34.0	40.0	23.0	41.0
64-65	33.56254963360906	37.0	34.0	40.0	22.0	41.0
66-67	33.22039579661045	36.0	33.5	39.0	22.0	41.0
68-69	32.86006862006768	36.0	33.0	39.0	21.0	40.5
70-71	32.79591275565798	35.5	33.5	39.0	21.5	40.0
72-73	32.4949540446646	35.0	33.0	37.5	20.5	40.0
74-75	32.167503042142656	35.0	33.0	37.0	20.0	39.0
76-77	31.857993499151483	35.0	33.0	36.5	19.5	39.0
78-79	31.56317755647715	35.0	33.0	36.0	18.0	38.5
80-81	31.288694720728227	35.0	33.0	36.0	17.5	37.0
82-83	31.06801163914545	35.0	33.0	35.0	16.5	37.0
84-85	30.854308043363616	35.0	32.5	35.0	13.5	36.5
86-87	30.67986963418363	35.0	32.0	35.0	10.5	36.0
88-89	30.542253463322943	35.0	32.0	35.0	8.5	36.0
90-91	30.421112059607527	35.0	32.0	35.0	7.0	35.5
92-93	30.31474531161185	35.0	32.0	35.0	7.0	35.0
94-95	30.198965363692515	35.0	32.0	35.0	3.5	35.0
96-97	30.11315898555572	35.0	32.0	35.0	2.0	35.0
98-99	30.033641583268526	35.0	32.0	35.0	2.0	35.0
100-101	29.946309516725115	35.0	32.0	35.0	2.0	35.0
102-103	29.136186415114473	33.5	30.0	35.0	2.0	35.0
104-105	29.72701874124037	34.0	31.0	35.0	2.0	35.0
106-107	29.80743852614601	35.0	31.0	35.0	2.0	35.0
108-109	29.753687507823386	35.0	31.0	35.0	2.0	35.0
110-111	29.680463185456816	35.0	31.0	35.0	2.0	35.0
112-113	29.603551868275737	35.0	31.0	35.0	2.0	35.0
114-115	29.482907057155344	34.5	31.0	35.0	2.0	35.0
116-117	29.362286357452277	34.0	31.0	35.0	2.0	35.0
118-119	29.360093244493875	34.5	31.0	35.0	2.0	35.0
120-121	29.522602658206807	35.0	32.0	35.0	2.0	35.0
122-123	29.405880312976457	35.0	32.0	35.0	2.0	35.0
124-125	28.86067550957172	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34115.0
3	4546.0
4	2935.0
5	2409.0
6	2588.0
7	2894.0
8	3024.0
9	3229.0
10	3547.0
11	4098.0
12	4226.0
13	4345.0
14	4448.0
15	3905.0
16	2945.0
17	2747.0
18	2561.0
19	2501.0
20	2408.0
21	2571.0
22	2893.0
23	3967.0
24	5965.0
25	7506.0
26	7475.0
27	8261.0
28	9831.0
29	11682.0
30	14198.0
31	17709.0
32	23472.0
33	31866.0
34	49640.0
35	82363.0
36	179697.0
37	326277.0
38	95727.0
39	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.915936963497565	17.393941197537565	11.349094853373533	25.341026985591338
2	16.932141526848994	19.063499506948382	38.419546392514235	25.58481257368839
3	21.09492361618467	22.690323348574523	27.86275129495399	28.35200174028682
4	13.610364876318851	14.503828543634725	34.29219658458198	37.59360999546445
5	14.330114500957853	37.96252117585373	32.96589186898521	14.741472454203208
6	35.13126849258552	33.50992480323324	16.50172366548061	14.857083038700628
7	30.52663551305968	31.750409942885057	18.816019275011648	18.90693526904362
8	28.061966344767576	35.51007717574336	18.125371333228667	18.302585146260398
9	27.743175409721683	15.98027425597498	17.642556055836252	38.633994278467085
10-11	27.00995776070694	25.52611853577976	26.049582044939978	21.414341658573324
12-13	26.200660080229554	22.575964891488987	26.688768529164147	24.534606499117313
14-15	23.629864829576093	24.154036925479524	23.622271850208985	28.5938263947354
16-17	24.356014549633436	27.440475274344728	24.36996906407277	23.833541111949067
18-19	24.147585020890965	26.765471216436964	25.905822381652072	23.181121381020002
20-21	24.779035286633352	25.75606152330724	25.750777249920482	23.71412594013893
22-23	24.62539279438737	25.27582182205292	25.798453191734964	24.300332191824747
24-25	24.160552522639133	25.466698885525062	25.939207939383397	24.433540652452404
26-27	24.04332252202705	25.749309714682067	25.811437958458725	24.39592980483215
28-29	24.13600271295379	26.420927503902945	25.584672837165822	23.858396945977436
30-31	24.729321518426246	25.816960602839355	25.730821639503056	23.722896239231346
32-33	24.26938553120929	25.59288755542809	25.936678967774107	24.201047945588506
34-35	24.304823040267934	25.937437151898475	25.90034394585521	23.857395861978386
36-37	24.288692861225204	26.165349345717487	25.79041822161159	23.755539571445723
38-39	24.334685032260182	26.251518609118225	25.72318628819097	23.69061007043063
40-41	24.29720647461714	25.816843239360747	25.958135597568173	23.927814688453942
42-43	24.124963381417615	25.88455360216546	25.731717515405457	24.258765501011467
44-45	23.792180500966317	26.10898169615212	26.001858243041976	24.096979559839582
46-47	23.9038085035647	25.821810137129113	25.765168958992607	24.50921240031358
48-49	23.86834590470249	26.069113628691248	25.792324607082723	24.270215859523546
50-51	23.936810415856495	26.08981613825697	25.927389027807564	24.04598441807897
52-53	24.030640671381825	26.184578698464065	25.83022346901816	23.95455716113595
54-55	23.82169016642477	25.716908077237015	26.122978561633186	24.338423194705026
56-57	24.035014272902828	26.020753683147714	25.62863363890274	24.315598405046718
58-59	23.942350462276767	26.19275922873324	25.96086410689339	23.9040262020966
60-61	24.35481810546902	25.982939483751878	25.93522708791753	23.727015322861575
62-63	24.202340379576754	26.06025843807444	25.97175927612303	23.765641906225778
64-65	24.091413491318786	25.771530913308936	25.900865427976168	24.236190167396106
66-67	23.982046004420265	26.032267530745884	25.754769639298914	24.230916825534937
68-69	23.805165401934474	25.986206372895825	25.921152742128534	24.287475483041167
70-71	23.85611224377766	25.866849033391375	26.18395576409845	24.093082958732516
72-73	23.909757849374103	26.29899445926534	25.65750051303099	24.13374717832957
74-75	23.847336578078323	26.211636243120907	25.81583344577245	24.12519373302832
76-77	23.840914186273558	25.863706252914675	25.956975908417203	24.338403652394565
78-79	23.770162541856465	25.81231168759212	26.119921753356856	24.29760401719456
80-81	23.91319180983954	26.10201942626193	25.717506054995415	24.267282708903114
82-83	24.117411364054956	26.23507001145609	25.75291878999436	23.89459983449459
84-85	24.133955767753772	26.345670689505678	25.550850870725828	23.96952267201472
86-87	23.74566222508614	26.42133313085253	25.74216737911178	24.090837264949545
88-89	23.67269020365927	26.088825751454326	25.88222679506892	24.356257249817485
90-91	24.112321758929113	25.81318560670967	25.958220508458403	24.116272125902814
92-93	23.985587438107096	26.26331810091974	25.81347706903531	23.937617391937852
94-95	23.896806829608654	26.056352480042683	25.774282254920067	24.272558435428596
96-97	23.717650752802037	26.037212729039496	25.94116928785225	24.30396723030622
98-99	23.774920363089926	26.012859905220665	25.75972509169339	24.45249463999602
100-101	23.650258858682296	26.132695921725198	25.93989224952838	24.277152970064126
102-103	23.498484748209634	26.130872402306405	25.992250078366247	24.378392771117717
104-105	23.76969029091148	26.190241660099815	25.860206117021278	24.179861931967427
106-107	23.686002133240848	26.175860517446864	25.89840581546697	24.23973153384532
108-109	23.63013716200097	26.173957432836282	25.953708145324512	24.24219725983824
110-111	23.570292539273733	26.40231076269534	25.85960987922879	24.16778681880214
112-113	23.56447031976589	26.531467656076206	25.834609086095867	24.06945293806203
114-115	23.699167826524587	26.684341995030863	25.636237432877447	23.980252745567103
116-117	23.523925418525526	26.38844996778205	25.832536435012088	24.255088178680335
118-119	23.565544079519988	26.311965710962156	25.8580223380216	24.26446787149626
120-121	23.47687426503937	26.39208982270646	26.036435576917157	24.09460033533701
122-123	23.48973112070916	26.62233825610918	25.757641828426376	24.130288794755288
124-125	23.477053715073712	26.46641166234363	25.794255015267424	24.262279607315232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	162.0
2	140.0
3	131.5
4	120.5
5	111.0
6	109.5
7	140.5
8	178.0
9	228.0
10	243.0
11	222.0
12	246.5
13	299.0
14	312.5
15	312.5
16	368.0
17	419.0
18	456.0
19	498.0
20	573.0
21	640.0
22	756.5
23	979.5
24	1201.5
25	1534.5
26	1924.0
27	2465.0
28	3137.5
29	3882.5
30	4949.5
31	6088.5
32	7394.5
33	9016.5
34	10703.0
35	12418.0
36	14474.0
37	16534.0
38	18265.5
39	20235.5
40	22589.0
41	24331.5
42	26016.0
43	29040.5
44	32221.0
45	35316.0
46	37640.5
47	39664.0
48	42753.5
49	44895.5
50	45680.0
51	45667.0
52	44866.0
53	43521.0
54	42600.0
55	40928.5
56	37694.5
57	34402.5
58	31312.0
59	28026.0
60	23546.0
61	19220.0
62	15515.5
63	11726.5
64	8629.5
65	6386.5
66	4776.0
67	3585.5
68	2715.0
69	2036.0
70	1515.5
71	1133.0
72	811.5
73	592.5
74	431.0
75	278.0
76	183.0
77	121.5
78	81.5
79	55.0
80	36.5
81	28.5
82	22.0
83	15.0
84	11.5
85	11.5
86	10.0
87	8.5
88	8.0
89	4.0
90	2.0
91	1.5
92	3.0
93	2.0
94	0.0
95	2.0
96	2.5
97	1.0
98	1.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01631368235721424
2	0.010157575807322074
3	0.009234159824838249
4	0.01231221309978433
5	0.006669115429049846
6	0.027189470595357063
7	0.011901805996458186
8	0.011594000668963579
9	0.008002938514859816
10-11	0.007746434075280975
12-13	0.006515212765302541
14-15	0.005899602110313326
16-17	0.004822283464082196
18-19	0.005950902998229093
20-21	0.005335292343239877
22-23	0.0055917967828187165
24-25	0.005899602110313326
26-27	0.004617079912419124
28-29	0.006002203886144862
30-31	0.005540495894902949
32-33	0.0070282216444602225
34-35	0.007182124308207526
36-37	0.006463911877386774
38-39	0.006771717204881383
40-41	0.006874318980712918
42-43	0.0070282216444602225
44-45	0.006720416316965613
46-47	0.008823752721512104
48-49	0.007489929635702135
50-51	0.005232690567408341
52-53	0.005643097670734485
54-55	0.005899602110313326
56-57	0.006976920756544454
58-59	0.006515212765302541
60-61	0.005386593231155644
62-63	0.0057970003344817896
64-65	0.0038475665936826037
66-67	0.004104071033261444
68-69	0.006310009213639469
70-71	0.0050274870157452686
72-73	0.004309274584924516
74-75	0.0041553719211772115
76-77	0.004976186127829501
78-79	0.003231955938693387
80-81	0.003437159490356459
82-83	0.005283991455324109
84-85	0.008362044730270192
86-87	0.0065665136532183106
88-89	0.004976186127829501
90-91	0.0050274870157452686
92-93	0.007746434075280975
94-95	0.006361310101555237
96-97	0.008515947394017497
98-99	0.006412610989471006
100-101	0.007746434075280975
102-103	0.005283991455324109
104-105	0.005130088791576805
106-107	0.0070282216444602225
108-109	0.006617814541134078
110-111	0.007387327859870598
112-113	0.005540495894902949
114-115	0.0026163452837041704
116-117	0.0030780532749460827
118-119	0.15421046907479877
120-121	0.01026017758315361
122-123	0.002308539956209562
124-125	0.0018468319649676496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	974642.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.91121661994904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.08087801565765	57.941155985324954
2	9.221617786624229	12.70945801760875
3	2.7097207726830925	5.601904654378208
4	1.3020331703292403	3.5889875938766913
5	0.709916084895939	2.4460590554125097
6	0.4677843713335349	1.9341354086635145
7	0.305697365469857	1.4746184160428732
8	0.22233765751705523	1.2257246783943867
9	0.1609148046433943	0.9979951464123955
>10	0.7749653102367128	9.020193704318295
>50	0.03161470529540284	1.4782366835410246
>100	0.012221861320262076	1.468549547278434
>500	2.980939936911984E-4	0.11298110874797945
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0260177583153609E-4	0.0
5	0.0	0.0	0.0	1.0260177583153609E-4	0.0
6	0.0	0.0	0.0	1.0260177583153609E-4	0.0
7	0.0	0.0	0.0	1.0260177583153609E-4	0.0
8	0.0	0.0	0.0	1.0260177583153609E-4	0.0
9	0.0	0.0	0.0	1.0260177583153609E-4	2.0520355166307218E-4
10-11	0.0	0.0	0.0	1.0260177583153609E-4	2.0520355166307218E-4
12-13	0.0	0.0	0.0	1.0260177583153609E-4	2.0520355166307218E-4
14-15	0.0	0.0	0.0	1.0260177583153609E-4	2.0520355166307218E-4
16-17	0.0	0.0	0.0	1.0260177583153609E-4	2.0520355166307218E-4
18-19	0.0	0.0	0.0	1.0260177583153609E-4	2.0520355166307218E-4
20-21	0.0	0.0	0.0	1.0260177583153609E-4	3.078053274946083E-4
22-23	0.0	0.0	0.0	3.078053274946083E-4	3.078053274946083E-4
24-25	0.0	0.0	0.0	3.591062154103763E-4	3.591062154103763E-4
26-27	0.0	0.0	0.0	5.643097670734485E-4	4.1040710332614436E-4
28-29	0.0	0.0	0.0	0.0025137435078726343	4.1040710332614436E-4
30-31	0.0	0.0	0.0	0.010721885574395521	4.1040710332614436E-4
32-33	0.0	0.0	0.0	0.021802877364201417	5.130088791576805E-4
34-35	0.0	0.0	0.0	0.03739834729059491	6.156106549892166E-4
36-37	0.0	0.0	0.0	0.06540863209260425	6.156106549892166E-4
38-39	0.0	0.0	0.0	0.1112716258893009	6.156106549892166E-4
40-41	0.0	0.0	0.0	0.17298659405196987	6.156106549892166E-4
42-43	0.0	0.0	0.0	0.2218763402356968	6.156106549892166E-4
44-45	0.0	0.0	0.0	0.2747162547889379	6.156106549892166E-4
46-47	0.0	0.0	0.0	0.3327888599095873	6.156106549892166E-4
48-49	0.0	0.0	0.0	0.39306740321061473	7.182124308207526E-4
50-51	0.0	0.0	0.0	0.46381132764645894	8.208142066522887E-4
52-53	0.0	0.0	0.0	0.5362994822714392	9.747168703995929E-4
54-55	0.0	0.0	0.0	0.6131995132571755	0.001128619534146897
56-57	0.0	0.0	0.0	0.6938958099486785	0.0012312213099784331
58-59	0.0	0.0	0.0	0.7868017179641346	0.0013338230858099692
60-61	0.0	0.0	0.0	0.896636918991794	0.0013338230858099692
62-63	0.0	0.0	0.0	1.013141235448503	0.0013338230858099692
64-65	0.0	0.0	0.0	1.1119980464621881	0.0013338230858099692
66-67	0.0	0.0	0.0	1.2232183714635734	0.0013338230858099692
68-69	0.0	0.0	0.0	1.3577806004666328	0.0013338230858099692
70-71	0.0	0.0	0.0	1.5060914674311183	0.0014364248616415053
72-73	0.0	0.0	0.0	1.6368061298404952	0.0015390266374730413
74-75	0.0	0.0	0.0	1.7685981108961033	0.0015390266374730413
76-77	0.0	0.0	0.0	1.9235267924017228	0.0015390266374730413
78-79	0.0	0.0	0.0	2.1110315377338553	0.0015390266374730413
80-81	0.0	0.0	0.0	2.2929444862831687	0.0015903275253888094
82-83	0.0	0.0	0.0	2.4879391612510027	0.0016416284133045774
84-85	0.0	0.0	0.0	2.681856517572606	0.0016416284133045774
86-87	0.0	5.1300887915768044E-5	0.0	2.887829582554415	0.0016929293012203455
88-89	0.0	1.0260177583153609E-4	0.0	3.139870844884583	0.0017442301891361135
90-91	0.0	1.0260177583153609E-4	0.0	3.4104317277523437	0.0017442301891361135
92-93	0.0	1.0260177583153609E-4	0.0	3.6690395037357306	0.0017442301891361135
94-95	0.0	1.0260177583153609E-4	0.0	3.9202599518592467	0.0017442301891361135
96-97	0.0	1.0260177583153609E-4	0.0	4.1987724723539515	0.0017442301891361135
98-99	0.0	1.0260177583153609E-4	0.0	4.515606756121735	0.0017442301891361135
100-101	0.0	1.0260177583153609E-4	0.0	4.862657262871906	0.0017442301891361135
102-103	0.0	1.0260177583153609E-4	0.0	5.235306912692045	0.0017442301891361135
104-105	0.0	1.0260177583153609E-4	0.0	5.609239084710078	0.0017442301891361135
106-107	0.0	1.0260177583153609E-4	0.0	5.989224761502172	0.0017442301891361135
108-109	0.0	1.0260177583153609E-4	0.0	6.387576156168111	0.0018981328528834178
110-111	0.0	1.0260177583153609E-4	0.0	6.835586810336514	0.002052035516630722
112-113	0.0	1.0260177583153609E-4	0.0	7.282161039643274	0.002052035516630722
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	885	0.0	58.495865	1
GTATCAA	1310	0.0	44.06052	1
GTACATG	4325	0.0	40.586826	1
TACATGG	4465	0.0	39.447495	2
ACATGGG	4535	0.0	38.182545	3
TCAACGC	1485	0.0	37.265392	4
CAACGCA	1525	0.0	36.28794	5
AACGCAG	1630	0.0	33.946884	6
CATGGGG	4310	0.0	33.686954	4
ATCAACG	1715	0.0	32.96163	3
ATGGGGG	2645	0.0	32.3956	5
TATCAAC	1745	0.0	31.712955	2
TGGGGGG	1750	0.0	29.91915	6
CCGTATA	100	4.4428903E-4	29.744574	9
GAGTACA	2980	0.0	28.35445	1
ACGCAGA	2265	0.0	28.110252	1
AGTACAT	2820	0.0	27.853128	2
GTATAGG	280	3.6379788E-12	27.627048	1
CGCAGAG	2375	0.0	26.808304	2
AGAGTAC	2430	0.0	26.446405	5
>>END_MODULE