FastQCFastQC Report
Mon 15 Aug 2016
SRR2921300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974642
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16320.1674460981570669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.1308172641852085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.10691105041646062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.062.4814221
GTATCAA18950.052.00961
GTACATG42050.039.7741431
TACATGG42700.039.3450972
ACATGGG43000.038.9320453
TCAACGC24350.037.6782844
CATGGGG38800.036.6974224
CAACGCA24750.036.347215
ATCAACG25800.035.791623
AACGCAG25650.035.5437056
TATCAAC27700.035.2722742
ATGGGGG23900.032.654485
GTGGTAT6900.028.5675931
ACGCAGA33800.027.1453657
CGCAGAG34950.026.7608268
GGGTTAG2057.479139E-826.2239131
ATGGGGC11150.025.6469525
GCGGTTA3103.2014214E-1023.1221581
GCAGAGT39600.022.8662789
GTGTACG2102.6965936E-622.7551381
Produced by FastQC (version 0.11.5)