##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	974642
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.445821132272158	33.0	30.0	34.0	23.0	34.0
2	30.181211152402625	33.0	31.0	34.0	26.0	34.0
3	30.415144227316286	33.0	31.0	34.0	27.0	34.0
4	32.67455024511564	37.0	35.0	37.0	19.0	37.0
5	32.325668296666876	37.0	35.0	37.0	19.0	37.0
6	32.465593520492654	37.0	35.0	37.0	17.0	37.0
7	32.654085294908285	37.0	35.0	37.0	17.0	37.0
8	32.90288536713994	37.0	35.0	37.0	17.0	37.0
9	34.65829504577065	39.0	37.0	39.0	22.0	39.0
10-11	34.84708487834507	39.0	37.0	39.0	26.5	39.0
12-13	34.84684684222515	39.0	37.0	39.0	26.5	39.0
14-15	36.26805893856411	41.0	38.0	41.0	27.0	41.0
16-17	36.28638823280753	41.0	38.0	41.0	27.0	41.0
18-19	36.32069005850353	41.0	38.0	41.0	27.0	41.0
20-21	36.316318709844225	41.0	38.0	41.0	27.0	41.0
22-23	36.276230143991334	41.0	38.0	41.0	27.0	41.0
24-25	36.23448815052091	41.0	38.0	41.0	27.0	41.0
26-27	36.14148374480065	41.0	38.0	41.0	27.0	41.0
28-29	36.068424611293175	40.5	38.0	41.0	26.0	41.0
30-31	35.97475278102114	40.0	37.5	41.0	25.0	41.0
32-33	35.84093031082182	40.0	37.0	41.0	25.0	41.0
34-35	35.76192335236938	40.0	37.0	41.0	24.5	41.0
36-37	35.68406809885066	40.0	37.0	41.0	24.0	41.0
38-39	35.589254310813615	40.0	37.0	41.0	24.0	41.0
40-41	35.46355020612697	40.0	36.0	41.0	22.5	41.0
42-43	35.3177494916082	40.0	36.0	41.0	21.0	41.0
44-45	35.16501648810538	40.0	36.0	41.0	18.5	41.0
46-47	34.95086657459868	40.0	35.0	41.0	14.5	41.0
48-49	34.728534169469405	40.0	35.0	41.0	9.0	41.0
50-51	34.548604513246914	40.0	35.0	41.0	7.5	41.0
52-53	34.36864766755383	39.5	35.0	41.0	6.5	41.0
54-55	34.17559473119361	39.0	35.0	41.0	2.0	41.0
56-57	33.93310877224663	39.0	35.0	41.0	2.0	41.0
58-59	33.879945662099516	39.0	35.0	41.0	2.0	41.0
60-61	33.64524153484049	38.5	35.0	41.0	2.0	41.0
62-63	33.36101460844084	37.5	34.5	40.5	2.0	41.0
64-65	33.059992797355335	37.0	34.0	40.0	2.0	41.0
66-67	32.7439326439862	36.5	34.0	39.5	2.0	41.0
68-69	32.406028059533654	36.0	34.0	39.0	2.0	41.0
70-71	32.0697799807519	35.5	34.0	39.0	2.0	41.0
72-73	31.714336135729837	35.0	33.5	37.5	2.0	40.0
74-75	31.374288200180168	35.0	33.0	37.0	2.0	39.0
76-77	31.06445546159513	35.0	33.0	36.5	2.0	39.0
78-79	30.797692896468654	35.0	33.0	36.0	2.0	38.0
80-81	30.561041387504336	35.0	33.0	36.0	2.0	37.0
82-83	30.34997517037025	35.0	33.0	35.0	2.0	37.0
84-85	30.16396635892974	35.0	33.0	35.0	2.0	36.0
86-87	30.01263797373805	35.0	32.0	35.0	2.0	36.0
88-89	29.884523240328242	35.0	32.0	35.0	2.0	36.0
90-91	29.776221422840386	35.0	32.0	35.0	2.0	36.0
92-93	29.706132610743225	35.0	32.0	35.0	2.0	35.0
94-95	29.63138978209435	35.0	32.0	35.0	2.0	35.0
96-97	29.571542166251813	35.0	32.0	35.0	2.0	35.0
98-99	29.513595248306558	35.0	32.0	35.0	2.0	35.0
100-101	29.45665382776445	35.0	32.0	35.0	2.0	35.0
102-103	28.789615571666314	34.0	30.0	35.0	2.0	35.0
104-105	29.328428797445625	35.0	31.5	35.0	2.0	35.0
106-107	29.389979602766964	35.0	32.0	35.0	2.0	35.0
108-109	29.461774682396204	35.0	32.0	35.0	2.0	35.0
110-111	29.564229224679423	35.0	33.0	35.0	2.0	35.0
112-113	29.529614463567135	35.0	33.0	35.0	2.0	35.0
114-115	29.467626574680754	35.0	32.5	35.0	2.0	35.0
116-117	29.40799903964738	35.0	32.0	35.0	2.0	35.0
118-119	29.332450274049343	35.0	32.0	35.0	2.0	35.0
120-121	29.24210479334976	35.0	32.0	35.0	2.0	35.0
122-123	29.138906388191764	35.0	32.0	35.0	2.0	35.0
124-125	28.62264759778462	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59918.0
3	3993.0
4	2944.0
5	2758.0
6	2777.0
7	3051.0
8	3192.0
9	3115.0
10	3405.0
11	3347.0
12	3751.0
13	4891.0
14	5755.0
15	5300.0
16	3404.0
17	3111.0
18	2584.0
19	2340.0
20	2079.0
21	2196.0
22	2489.0
23	3046.0
24	4006.0
25	5199.0
26	6179.0
27	7076.0
28	8426.0
29	9890.0
30	11981.0
31	14982.0
32	19298.0
33	26041.0
34	39739.0
35	66705.0
36	151539.0
37	342538.0
38	131287.0
39	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32061409785331	17.43828701620799	11.012435769799787	27.228663116138907
2	16.198460562955425	19.30996201682259	37.20032586324004	27.291251556981948
3	19.977796975710056	22.258634452445104	28.198764264211885	29.564804307632958
4	13.359083343424	15.163089098181834	31.458658714538153	40.019168843856015
5	13.733977916450845	37.660118063948566	32.72194542419982	15.883958595400768
6	34.979409083817146	33.56093515195809	15.976539083735025	15.483116680489747
7	30.46358023677772	31.522470895387595	18.150666655719938	19.863282212114743
8	28.508498750711865	35.04655971186849	17.624556849023403	18.820384688396242
9	27.55464210332978	15.89338466542646	17.540266517814544	39.01170671342922
10-11	27.14721416350517	25.30443994695355	25.32885978345351	22.219486106087775
12-13	26.303045920046074	22.35893903283479	26.356173327782763	24.981841719336376
14-15	23.572193687528344	23.71342503196045	23.739178077694167	28.975203202817035
16-17	23.905546857205003	27.04972697667451	24.457749614730332	24.58697655139015
18-19	23.878972997264636	26.001188128564127	26.517275060996752	23.60256381317448
20-21	24.407269541021215	25.158878849875133	26.441862755760575	23.991988853343074
22-23	24.22089341522323	24.66613382144418	26.368297282489365	24.744675480843224
24-25	23.9029305119213	25.01359473529768	26.413134258527748	24.670340494253274
26-27	23.74907914906191	25.210384941342568	26.445505118802597	24.595030790792926
28-29	23.81361566605995	25.833331623303735	26.17463643060734	24.178416280028976
30-31	24.504536024509513	25.38414104871327	26.237377416528325	23.87394551024889
32-33	24.01071367743233	25.239729049230387	26.464794252658923	24.284763020678362
34-35	24.069555795871715	25.474994921212097	26.564677081430926	23.890772201485262
36-37	24.146968835736608	25.504287728212	26.556674142916066	23.792069293135327
38-39	24.020614748800075	25.709388678099245	26.501063980415374	23.768932592685314
40-41	23.902314901266312	25.62746116009776	26.530254185639446	23.939969752996486
42-43	23.856451907469616	25.676145702729826	26.229990088668455	24.237412301132107
44-45	23.598613644804963	25.820147295109386	26.47613174888831	24.105107311197344
46-47	23.605385362009844	25.72575366134437	26.066545459768818	24.602315516876967
48-49	23.76826568114241	25.907256202790357	25.93136762011077	24.393110495956464
50-51	23.748309635743176	26.014423757646398	26.006010412028214	24.231256194582215
52-53	23.940841868091052	26.140931747246682	25.89330236127727	24.024924023384997
54-55	23.60435934425153	25.60160551258821	26.32263949224433	24.471395650915927
56-57	23.718144713648705	25.86724151021606	25.882016165935802	24.53259761019944
58-59	23.65684015258936	25.969176374504688	26.13995703037628	24.234026442529668
60-61	24.160101863043046	25.709696483426736	26.171558377332392	23.958643276197826
62-63	23.90949702557452	25.833331623303735	26.240609372467016	24.016561978654728
64-65	23.747232317096945	25.476739151401233	26.183614085992602	24.592414445509224
66-67	23.631651416622717	25.85487799622836	25.970920604693827	24.542549982455096
68-69	23.425986156968403	25.82697031320218	26.207366397097605	24.539677132731814
70-71	23.615081229825925	25.6584981972868	26.2672345332953	24.459186039591973
72-73	23.571834581312935	26.07393278762869	25.854724093564613	24.499508537493767
74-75	23.474722000488384	25.96609832122974	25.971690118012564	24.58748956026931
76-77	23.52058499428508	25.610788371525135	26.112870161556756	24.755756472633028
78-79	23.388074800798652	25.536350783159357	26.311712403118275	24.76386201292372
80-81	23.578965404733225	25.771975761356476	25.90746140634202	24.741597427568276
82-83	23.64411753238625	25.94434674475346	25.90156180423171	24.50997391862858
84-85	23.69454630520745	25.956761559629076	25.803115400321346	24.545576734842125
86-87	23.273879024298154	26.164786660127515	25.93167542543826	24.62965889013607
88-89	23.207341772671402	25.81122094061204	26.030429634676118	24.951007652040442
90-91	23.56781514376869	25.568710860488274	26.123172848189352	24.740301147553684
92-93	23.33957326654604	25.993024065895337	26.02857578184277	24.638826885715854
94-95	23.452813346466385	25.7647952064352	25.82974226381023	24.952649183288187
96-97	23.23157237002084	25.737017987209597	25.928525184248087	25.10288445852148
98-99	23.125477419133087	25.67549196294227	25.923942290575557	25.27508832734908
100-101	23.12192579429165	25.745709706743604	26.07157294678456	25.060791552180184
102-103	22.961815723106536	25.755662078999265	26.03125044888277	25.251271749011433
104-105	23.190732182038214	25.76860312227624	25.946514692838342	25.094150002847197
106-107	23.12767951637503	25.64399452310316	26.051375918993635	25.176950041528173
108-109	23.039769185742582	25.64910097251642	26.058022657678016	25.253107184062983
110-111	22.935473892061296	26.01672520986029	25.902580090115645	25.145220807962765
112-113	22.974762282193545	25.969891215406832	25.970865941778055	25.084480560621568
114-115	22.966692744738427	25.984378807505355	25.82575572969437	25.223172718061843
116-117	22.683939466538483	25.729388811908464	26.01477784749052	25.571893874062535
118-119	22.80226094201129	25.651062240544693	26.051056905230457	25.495619912213563
120-121	22.623931593238737	25.69768032418107	26.226593292603784	25.451794789976407
122-123	22.660130112048627	25.87312100133231	25.92319172109378	25.543557165525282
124-125	22.489124189444308	25.70980054173849	25.879709431174586	25.921365837642618
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	35.5
2	47.5
3	52.0
4	48.0
5	64.0
6	144.0
7	328.5
8	473.5
9	568.0
10	556.5
11	511.0
12	541.5
13	568.0
14	651.5
15	704.5
16	741.0
17	789.0
18	836.0
19	895.5
20	937.0
21	987.0
22	1071.5
23	1251.5
24	1470.5
25	1718.0
26	2083.0
27	2516.5
28	3022.0
29	3763.0
30	4701.0
31	5739.5
32	6877.5
33	8213.5
34	9833.0
35	11491.0
36	13416.5
37	15626.5
38	17646.0
39	19438.5
40	21522.0
41	23186.0
42	24999.0
43	28218.5
44	31225.0
45	34384.5
46	36783.0
47	38823.5
48	41751.0
49	44016.0
50	45379.0
51	45921.0
52	45561.0
53	44416.0
54	43532.5
55	41836.0
56	38720.5
57	35167.0
58	31924.0
59	28599.0
60	24173.0
61	19932.0
62	16062.0
63	12086.0
64	8858.0
65	6587.0
66	4923.0
67	3735.5
68	2845.5
69	2087.5
70	1596.0
71	1262.0
72	931.0
73	691.0
74	521.5
75	350.5
76	253.5
77	192.0
78	143.0
79	113.0
80	93.5
81	88.0
82	80.0
83	69.0
84	56.0
85	59.0
86	56.0
87	56.5
88	58.0
89	41.0
90	37.0
91	41.0
92	39.0
93	43.5
94	41.5
95	30.0
96	22.5
97	20.0
98	17.0
99	8.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3417665152948467
2	0.0
3	0.0
4	0.014774655719741197
5	0.16518885908877312
6	0.04370835650423437
7	0.023188001337927158
8	0.00892635449734364
9	0.007489929635702135
10-11	0.0025137435078726343
12-13	0.05832910956022827
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.591062154103763E-4
92-93	5.643097670734485E-4
94-95	2.052035516630722E-4
96-97	5.130088791576805E-4
98-99	5.130088791576805E-5
100-101	0.0
102-103	0.0
104-105	5.130088791576805E-5
106-107	2.5650443957884024E-4
108-109	5.643097670734485E-4
110-111	5.643097670734485E-4
112-113	9.747168703995929E-4
114-115	4.617079912419124E-4
116-117	7.695133187365207E-4
118-119	4.104071033261444E-4
120-121	1.5390266374730414E-4
122-123	0.00210333640454649
124-125	2.052035516630722E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	974642.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.67365393636369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17656257220277	59.345623441446826
2	8.558352490245854	11.925833793416121
3	2.5358460281366497	5.300449758008859
4	1.1834973642701754	3.2983434317103497
5	0.6780249874974688	2.3620239169552977
6	0.41717257296175075	1.7439562488167668
7	0.28475187683957787	1.3887792607255562
8	0.2119779950895477	1.1815425177594676
9	0.15511710441443674	0.9726817907324032
>10	0.753782076404065	8.958149806616474
>50	0.032843992194634	1.5643580411347808
>100	0.011040494031234707	1.323078097374215
>500	5.888261210387615E-4	0.2301539940351574
>1k	4.416195907790711E-4	0.4050259012677525
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1632	0.1674460981570669	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1275	0.1308172641852085	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1042	0.10691105041646062	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.0260177583153609E-4	0.0
14-15	0.0	0.0	0.0	1.0260177583153609E-4	0.0
16-17	0.0	0.0	0.0	1.0260177583153609E-4	0.0
18-19	0.0	0.0	0.0	1.0260177583153609E-4	0.0
20-21	0.0	0.0	0.0	1.0260177583153609E-4	0.0
22-23	0.0	0.0	0.0	3.078053274946083E-4	1.5390266374730414E-4
24-25	0.0	0.0	0.0	3.591062154103763E-4	3.078053274946083E-4
26-27	0.0	0.0	0.0	5.643097670734485E-4	3.078053274946083E-4
28-29	0.0	0.0	0.0	0.0024624426199568662	3.078053274946083E-4
30-31	0.0	0.0	0.0	0.010106274919406305	3.078053274946083E-4
32-33	0.0	0.0	0.0	0.02046905427839145	3.078053274946083E-4
34-35	0.0	0.0	0.0	0.035602816213543026	6.156106549892166E-4
36-37	0.0	0.0	0.0	0.06166366727475319	6.156106549892166E-4
38-39	0.0	0.0	0.0	0.10511551933940871	6.156106549892166E-4
40-41	0.0	0.0	0.0	0.16277771735673202	6.156106549892166E-4
42-43	0.0	0.0	0.0	0.20920502092050208	6.156106549892166E-4
44-45	0.0	0.0	0.0	0.26014680262085976	6.156106549892166E-4
46-47	0.0	0.0	0.0	0.3155517615698893	6.156106549892166E-4
48-49	0.0	0.0	0.0	0.37234184449264446	7.182124308207526E-4
50-51	0.0	0.0	0.0	0.4391356005589745	7.182124308207526E-4
52-53	0.0	0.0	0.0	0.5079813921419352	7.695133187365207E-4
54-55	0.0	0.0	0.0	0.5810851574219047	8.208142066522887E-4
56-57	0.0	0.0	0.0	0.659319011493451	8.208142066522887E-4
58-59	0.0	0.0	0.0	0.7482747511393928	8.208142066522887E-4
60-61	0.0	0.0	0.0	0.851492137625918	8.208142066522887E-4
62-63	0.0	0.0	0.0	0.9630202679547978	8.208142066522887E-4
64-65	0.0	0.0	0.0	1.0569008928406531	8.208142066522887E-4
66-67	0.0	0.0	0.0	1.1619138104042306	8.208142066522887E-4
68-69	0.0	0.0	0.0	1.2883704991165987	8.208142066522887E-4
70-71	0.0	0.0	0.0	1.4276011089199931	8.208142066522887E-4
72-73	0.0	0.0	0.0	1.5511849479090785	8.208142066522887E-4
74-75	0.0	0.0	0.0	1.6768721233027102	8.721150945680567E-4
76-77	0.0	0.0	0.0	1.8254394947067745	9.234159824838248E-4
78-79	0.0	0.0	0.0	2.002889266007416	9.234159824838248E-4
80-81	0.0	0.0	0.0	2.17315691299985	9.747168703995929E-4
82-83	0.0	0.0	0.0	2.3574810032812046	0.0012312213099784331
84-85	0.0	0.0	0.0	2.5393426509426025	0.0012312213099784331
86-87	0.0	0.0	0.0	2.7324904939454693	0.0012825221978942012
88-89	0.0	0.0	0.0	2.971603932520864	0.0013338230858099692
90-91	0.0	0.0	0.0	3.2266206463501472	0.0013338230858099692
92-93	0.0	0.0	0.0	3.4721979968029286	0.0013338230858099692
94-95	0.0	0.0	0.0	3.7113114353783234	0.0013338230858099692
96-97	0.0	0.0	0.0	3.978589061419475	0.0013851239737257372
98-99	0.0	0.0	0.0	4.283059831199559	0.0014364248616415053
100-101	0.0	0.0	0.0	4.611026407645063	0.0014364248616415053
102-103	0.0	0.0	0.0	4.963566109402222	0.0014364248616415053
104-105	0.0	0.0	0.0	5.317388333357274	0.0014364248616415053
106-107	0.0	0.0	0.0	5.678700486948028	0.0014364248616415053
108-109	0.0	0.0	0.0	6.057249738878481	0.0014364248616415053
110-111	0.0	0.0	0.0	6.4800716570802415	0.0014364248616415053
112-113	0.0	0.0	0.0	6.907715858746084	0.0014364248616415053
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1195	0.0	62.481422	1
GTATCAA	1895	0.0	52.0096	1
GTACATG	4205	0.0	39.774143	1
TACATGG	4270	0.0	39.345097	2
ACATGGG	4300	0.0	38.932045	3
TCAACGC	2435	0.0	37.678284	4
CATGGGG	3880	0.0	36.697422	4
CAACGCA	2475	0.0	36.34721	5
ATCAACG	2580	0.0	35.79162	3
AACGCAG	2565	0.0	35.543705	6
TATCAAC	2770	0.0	35.272274	2
ATGGGGG	2390	0.0	32.65448	5
GTGGTAT	690	0.0	28.567593	1
ACGCAGA	3380	0.0	27.145365	7
CGCAGAG	3495	0.0	26.760826	8
GGGTTAG	205	7.479139E-8	26.223913	1
ATGGGGC	1115	0.0	25.646952	5
GCGGTTA	310	3.2014214E-10	23.122158	1
GCAGAGT	3960	0.0	22.866278	9
GTGTACG	210	2.6965936E-6	22.755138	1
>>END_MODULE