FastQCFastQC Report
Mon 15 Aug 2016
SRR2921299_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921299_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458853
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24350.050.338671
GGTATCA14550.050.3008841
TCAACGC29750.040.801554
CAACGCA30200.040.1935845
GTACATG77350.038.3091471
AACGCAG31850.038.1100356
TACATGG80250.036.702332
ATCAACG33800.036.2646943
ACATGGG81000.035.9217343
TATCAAC34850.035.001332
CATGGGG73050.031.930034
ATGGGGG45650.031.0219885
ACGCAGA41100.029.5289087
GTGGTAT7850.028.0456521
CGCAGAG43800.027.8444618
TGGGGGG33100.025.7055346
GAGTACA53850.023.7567081
GCAGAGT51500.022.8726779
AGTACAT54050.022.5679262
AGAGTAC45000.022.4786345
Produced by FastQC (version 0.11.5)