##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921299_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1458853
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59710745359539	33.0	31.0	34.0	27.0	34.0
2	30.61961074899253	33.0	31.0	34.0	27.0	34.0
3	30.77131006345396	33.0	31.0	34.0	27.0	34.0
4	34.22124984491241	37.0	35.0	37.0	31.0	37.0
5	34.21441502330941	37.0	35.0	37.0	31.0	37.0
6	34.28100363778941	37.0	35.0	37.0	31.0	37.0
7	34.161539922116894	37.0	35.0	37.0	30.0	37.0
8	34.154428170624456	37.0	35.0	37.0	30.0	37.0
9	35.760858702007674	39.0	37.0	39.0	30.0	39.0
10-11	35.80190567521197	39.0	37.0	39.0	30.5	39.0
12-13	35.75794716808342	39.0	37.0	39.0	30.0	39.0
14-15	37.090237330286186	41.0	38.0	41.0	31.0	41.0
16-17	37.07495511885022	41.0	38.0	41.0	31.0	41.0
18-19	37.05994401080849	41.0	38.0	41.0	30.5	41.0
20-21	37.09771066721596	41.0	38.0	41.0	31.0	41.0
22-23	37.043264125994874	41.0	38.0	41.0	30.0	41.0
24-25	37.02408433200603	41.0	38.0	41.0	30.0	41.0
26-27	36.99310245788986	41.0	38.0	41.0	30.0	41.0
28-29	36.93371162139023	40.0	38.0	41.0	30.0	41.0
30-31	36.86007431865993	40.0	38.0	41.0	30.0	41.0
32-33	36.79036236001845	40.0	38.0	41.0	30.0	41.0
34-35	36.68423857647069	40.0	38.0	41.0	30.0	41.0
36-37	36.573070419020965	40.0	37.5	41.0	30.0	41.0
38-39	36.47403371004481	40.0	37.0	41.0	29.0	41.0
40-41	36.37078273136498	40.0	37.0	41.0	29.0	41.0
42-43	36.17007779399295	40.0	36.5	41.0	28.0	41.0
44-45	35.915690957210906	40.0	36.0	41.0	27.5	41.0
46-47	35.69652014287937	40.0	35.5	41.0	27.0	41.0
48-49	35.48541696798787	40.0	35.0	41.0	26.0	41.0
50-51	35.308036519100966	39.0	35.0	41.0	26.0	41.0
52-53	35.11457083064572	39.0	35.0	41.0	26.0	41.0
54-55	34.898879462152806	39.0	35.0	41.0	26.0	41.0
56-57	34.64864794465241	38.5	35.0	40.0	25.0	41.0
58-59	34.369988614342915	38.0	34.0	40.0	24.0	41.0
60-61	34.07280514212192	37.5	34.0	40.0	23.0	41.0
62-63	33.740343955148326	37.0	34.0	40.0	23.0	41.0
64-65	33.380399190322805	36.5	34.0	39.0	22.0	41.0
66-67	33.02914618539359	36.0	33.0	39.0	22.0	41.0
68-69	32.67378378767429	35.0	33.0	38.5	20.5	40.0
70-71	32.615296743400464	35.0	33.0	38.0	21.5	40.0
72-73	32.325709992713456	35.0	33.0	37.0	20.5	39.5
74-75	32.001589262249176	35.0	33.0	37.0	20.0	39.0
76-77	31.71372304132082	35.0	33.0	36.0	20.0	39.0
78-79	31.438103770564958	35.0	33.0	36.0	18.5	37.5
80-81	31.17948038630349	35.0	33.0	35.5	18.0	37.0
82-83	30.973336244296032	35.0	32.5	35.0	17.0	37.0
84-85	30.772788965029378	35.0	32.0	35.0	14.5	36.0
86-87	30.61393334352399	35.0	32.0	35.0	12.0	36.0
88-89	30.48674712256821	35.0	32.0	35.0	10.0	36.0
90-91	30.378850028069998	35.0	32.0	35.0	7.5	35.0
92-93	30.26906240724734	35.0	32.0	35.0	7.0	35.0
94-95	30.15656169607219	35.0	32.0	35.0	7.0	35.0
96-97	30.072140921669284	35.0	32.0	35.0	3.5	35.0
98-99	29.99528944999942	35.0	31.5	35.0	2.0	35.0
100-101	29.909869260302443	35.0	31.0	35.0	2.0	35.0
102-103	29.069497749259178	33.5	29.5	34.5	2.0	35.0
104-105	29.676855721584012	34.0	31.0	35.0	2.0	35.0
106-107	29.764099261543144	35.0	31.0	35.0	2.0	35.0
108-109	29.702437120121083	35.0	31.0	35.0	2.0	35.0
110-111	29.628372426831216	35.0	31.0	35.0	2.0	35.0
112-113	29.542933043973587	34.0	31.0	35.0	2.0	35.0
114-115	29.421154838767166	34.0	31.0	35.0	2.0	35.0
116-117	29.297807935412273	34.0	31.0	35.0	2.0	35.0
118-119	29.30244582559038	34.5	31.0	35.0	2.0	35.0
120-121	29.47785691910014	35.0	32.0	35.0	2.0	35.0
122-123	29.354031557668936	35.0	32.0	35.0	2.0	35.0
124-125	28.787731868803778	34.5	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49880.0
3	7243.0
4	4415.0
5	3791.0
6	3981.0
7	4532.0
8	4726.0
9	4916.0
10	5501.0
11	6052.0
12	5996.0
13	6065.0
14	5969.0
15	5290.0
16	4303.0
17	3873.0
18	3767.0
19	3639.0
20	3689.0
21	3909.0
22	4456.0
23	6036.0
24	8389.0
25	10750.0
26	11381.0
27	12795.0
28	15273.0
29	18296.0
30	22014.0
31	27734.0
32	36157.0
33	50131.0
34	79494.0
35	132986.0
36	286167.0
37	473448.0
38	121758.0
39	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.62969018985975	17.748352361814696	12.380065034831274	25.241892413494284
2	16.408636044854344	18.84892954368925	39.293825843555496	25.448608567900916
3	20.17698168620714	23.06239468232281	28.192231068869024	28.56839256260103
4	13.660935075868567	14.244423405073627	34.359928179723454	37.734713339334355
5	14.605231857858653	37.91036918058815	32.47408510355333	15.010313857999872
6	35.4897190673794	33.21960357316234	16.503934373174403	14.786742986283862
7	31.07061422211375	31.438555165090104	18.91464620219407	18.576184410602075
8	27.958216617100828	35.54597145611127	18.23378347065058	18.26202845613732
9	28.090298962782185	15.797314065167168	17.807446305297145	38.3049406667535
10-11	27.17004090908312	25.21387721105282	25.933410912630745	21.682670967233317
12-13	26.54066984497928	22.422802410709785	26.543549041107724	24.492978703203207
14-15	24.11007529064327	23.910554743214554	23.568651726447424	28.410718239694756
16-17	24.73024520172185	26.918548635039517	24.239800627716956	24.111405535521673
18-19	24.635364306644625	26.48167044330062	25.45640944081939	23.42655580923537
20-21	25.386321259151607	25.340151909841236	25.341522937289206	23.93200389371795
22-23	25.383236972822086	24.757190706066392	25.30059818009567	24.558974141015852
24-25	24.924687567801755	25.004927141712823	25.22573164198412	24.844653648501307
26-27	24.54089063053225	25.228727792590426	25.43934964633009	24.79103193054723
28-29	24.622493443870933	25.75281088356843	25.254473094935083	24.37022257762556
30-31	25.32896980548871	25.352962810499474	25.11382110198501	24.204246282026805
32-33	24.886312805598067	25.326391162099583	25.189421529528506	24.597874502773838
34-35	24.854804809127966	25.548800802892384	25.376081594487243	24.220312793492404
36-37	24.882226643102857	25.48350144714526	25.345642180137023	24.288629729614858
38-39	24.923221831323847	25.613916865695792	25.17703537554122	24.28582592743915
40-41	24.884875078963614	25.381193309438306	25.28450037241005	24.449431239188026
42-43	24.61799910675939	25.386096912150162	25.176977151755274	24.818926829335172
44-45	24.466548870337775	25.36938746480249	25.504436214316193	24.659627450543535
46-47	24.463694375804863	25.287300769551653	25.427219252125084	24.821785602518396
48-49	24.416947819824784	25.532477174408193	25.34419624816064	24.706378757606387
50-51	24.442889846859188	25.60823249680978	25.417659093658944	24.531218562672088
52-53	24.665702153154736	25.50452728383878	25.28728895268708	24.542481610319403
54-55	24.25535561268209	25.247060839760067	25.663410454155954	24.834173093401883
56-57	24.471960911002608	25.46796940473599	25.222069310646816	24.838000373614584
58-59	24.546748317208653	25.528042409289682	25.42356847053307	24.5016408029686
60-61	24.944824922168777	25.510097407705352	25.393526238452996	24.15155143167288
62-63	24.697276309403353	25.560237627128867	25.377890476543026	24.36459558692475
64-65	24.552188953794257	25.235597572098257	25.399089176958583	24.813124297148907
66-67	24.546567858784382	25.481931778415063	25.266410769743842	24.705089593056716
68-69	24.366945320295144	25.392179075030825	25.45966880332725	24.781206801346777
70-71	24.36058398413735	25.35741522410109	25.564301493207473	24.717699298554095
72-73	24.4836620662566	25.847058815464674	25.091120475080942	24.57815864319779
74-75	24.368043647326694	25.718312552890815	25.31972701912244	24.59391678066005
76-77	24.33954904659797	25.31930879038317	25.329420006909903	25.011722156108956
78-79	24.204740742568724	25.361341265875193	25.611443918670197	24.822474072885882
80-81	24.48718963778093	25.637587619134777	25.17926555362817	24.695957189456124
82-83	24.59840131728001	25.611245414352634	25.326449421033637	24.463903847333718
84-85	24.6318227827188	25.762516311540395	24.989228470566218	24.616432435174588
86-87	24.23160016987385	25.959165150221473	25.214441745368145	24.594792934536528
88-89	24.141862076860697	25.518193764742737	25.43260790433578	24.907336254060787
90-91	24.502987115813717	25.334341035053626	25.483028452149593	24.679643396983064
92-93	24.608331039664417	25.496400796448683	25.380820449083807	24.514447714803094
94-95	24.679020275759097	25.46044969038285	25.201904112941538	24.658625920916517
96-97	24.323713822288582	25.410022060708766	25.373277064129617	24.892987052873032
98-99	24.144664523950627	25.514716291211492	25.30871666345042	25.031902521387455
100-101	24.25391526103454	25.576517688513523	25.377746852538017	24.791820197913918
102-103	24.021197462638632	25.536560344594157	25.480348199960307	24.961893992806903
104-105	24.233788900093163	25.592640095861647	25.28906221615623	24.88450878788897
106-107	24.30254494999767	25.434452661626672	25.462902027240098	24.80010036113556
108-109	24.17871618437176	25.446593372693854	25.44169187396636	24.932998568968028
110-111	24.102732497418113	25.907361965503327	25.364351841219758	24.6255536958588
112-113	24.094146890207664	25.856393396322773	25.404332300723283	24.64512741274628
114-115	24.154970200015836	26.057000588493562	25.246785135413504	24.541244076077103
116-117	24.00969423935832	25.951763927243164	25.300545755102892	24.73799607829562
118-119	24.07456291962925	25.734285754497645	25.37711065425347	24.814040671619633
120-121	23.946609354674507	25.81956662947557	25.578355810808727	24.655468205041192
122-123	23.938781987347298	26.02454451169062	25.297038220642865	24.739635280319217
124-125	23.9052513387459	25.940172522051945	25.237656529762248	24.916919609439905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	299.0
1	273.5
2	223.0
3	183.5
4	152.5
5	149.5
6	155.5
7	171.5
8	212.0
9	239.0
10	236.0
11	238.5
12	268.5
13	302.0
14	338.5
15	366.5
16	392.5
17	446.0
18	466.0
19	477.0
20	518.5
21	597.0
22	708.0
23	831.0
24	1014.0
25	1224.0
26	1637.5
27	2277.0
28	2967.0
29	3810.5
30	5161.5
31	6846.5
32	8810.0
33	10903.5
34	13235.5
35	15718.0
36	18789.5
37	22136.5
38	25044.0
39	28353.0
40	31387.5
41	34194.5
42	37415.5
43	41505.5
44	45893.5
45	49878.5
46	53760.0
47	57613.0
48	62056.0
49	65768.5
50	67480.5
51	68914.5
52	69313.0
53	68734.5
54	68230.0
55	66393.0
56	62634.5
57	57360.5
58	51994.0
59	46121.0
60	39523.5
61	33359.0
62	27243.0
63	20613.5
64	14825.5
65	10862.5
66	7978.5
67	5707.5
68	4101.0
69	3137.0
70	2461.0
71	1774.5
72	1255.0
73	983.5
74	726.0
75	525.0
76	370.0
77	220.0
78	133.0
79	91.5
80	66.0
81	44.5
82	39.0
83	31.0
84	18.0
85	16.0
86	10.5
87	5.5
88	11.0
89	11.0
90	7.5
91	5.0
92	5.5
93	5.0
94	4.5
95	4.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01713675058419183
2	0.012064272411271048
3	0.01172153739958721
4	0.014531964495394671
5	0.009322392317800354
6	0.029132475993126107
7	0.013366665455669625
8	0.012818289436975487
9	0.009802221334157726
10-11	0.008945383804948134
12-13	0.007711537762886322
14-15	0.0072659822476973356
16-17	0.005106751674089164
18-19	0.007026067739518649
20-21	0.00637487121731936
22-23	0.006511965221992894
24-25	0.006614785725498045
26-27	0.005312392681099466
28-29	0.0070946147418554165
30-31	0.006477691720824511
32-33	0.00928811881663197
34-35	0.008705469296769448
36-37	0.00815709327807531
38-39	0.007197435245360567
40-41	0.007642990760549555
42-43	0.008191366779243694
44-45	0.00825991378158046
46-47	0.010042135842336411
48-49	0.008808289800274599
50-51	0.0066833327278348125
52-53	0.005929315702130372
54-55	0.007060341240687033
56-57	0.008945383804948134
58-59	0.007951452271065008
60-61	0.00599786270446714
62-63	0.006649059226666429
64-65	0.004284187646047957
66-67	0.004284187646047957
68-69	0.007711537762886322
70-71	0.005518033688109768
72-73	0.004798290163573712
74-75	0.004764016662405328
76-77	0.0052781191799310834
78-79	0.0035644441215119
80-81	0.0036672646250170506
82-83	0.006134956709140674
84-85	0.010042135842336411
86-87	0.008465554788590762
88-89	0.005757948196288454
90-91	0.006032136205635524
92-93	0.008739742797937832
94-95	0.007951452271065008
96-97	0.010350597352851864
98-99	0.007711537762886322
100-101	0.00945948632247389
102-103	0.0064091447184877436
104-105	0.005757948196288454
106-107	0.008294187282748845
108-109	0.007917178769896624
110-111	0.009082477809621669
112-113	0.006169230210309058
114-115	0.0029817946016493777
116-117	0.0033245296133332146
118-119	0.1565956268383449
120-121	0.011687263898418827
122-123	0.0023305980794500886
124-125	0.001713675058419183
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1458853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.005886002379185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60531202732327	49.17398476725087
2	10.326643808923592	12.59972109988735
3	3.439762575829181	6.295372907288558
4	1.665599899130614	4.0644559028774605
5	0.9502045200206208	2.898403431366171
6	0.6119445109506216	2.239933024490118
7	0.44061521815513705	1.8816085238780005
8	0.3224059466290374	1.5734928341232162
9	0.24877483160706781	1.3659056115553672
>10	1.3148259379558054	14.04649016082149
>50	0.05922591677307333	2.3896067951566504
>100	0.01446062519452685	1.3381587979908138
>500	1.1209075373716592E-4	0.05347469072128857
>1k	1.1209075373716592E-4	0.07939145259260362
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.854700233676731E-5	0.0
6	0.0	0.0	0.0	6.854700233676731E-5	0.0
7	0.0	0.0	0.0	6.854700233676731E-5	0.0
8	0.0	0.0	0.0	6.854700233676731E-5	0.0
9	0.0	0.0	0.0	6.854700233676731E-5	0.0
10-11	0.0	0.0	0.0	1.0282050350515098E-4	0.0
12-13	0.0	0.0	0.0	1.3709400467353463E-4	6.854700233676731E-5
14-15	0.0	0.0	0.0	1.3709400467353463E-4	1.0282050350515098E-4
16-17	0.0	0.0	0.0	2.0564100701030193E-4	1.3709400467353463E-4
18-19	0.0	0.0	0.0	2.3991450817868558E-4	1.3709400467353463E-4
20-21	0.0	0.0	0.0	3.4273501168383656E-4	1.3709400467353463E-4
22-23	0.0	0.0	0.0	5.483760186941384E-4	1.3709400467353463E-4
24-25	0.0	0.0	0.0	8.225640280412077E-4	2.7418800934706926E-4
26-27	0.0	0.0	0.0	9.93931533883126E-4	2.7418800934706926E-4
28-29	0.0	0.0	0.0	0.0027076065923023088	2.7418800934706926E-4
30-31	0.0	0.0	0.0	0.01031632385168348	2.7418800934706926E-4
32-33	0.0	0.0	0.0	0.026116407890308342	2.7418800934706926E-4
34-35	0.0	0.0	0.0	0.04602931206913925	2.7418800934706926E-4
36-37	0.0	0.0	0.0	0.07553879657511758	2.7418800934706926E-4
38-39	0.0	0.0	0.0	0.12681195432301953	2.7418800934706926E-4
40-41	0.0	0.0	0.0	0.1887441709342888	2.7418800934706926E-4
42-43	0.0	0.0	0.0	0.24389023431421808	2.7418800934706926E-4
44-45	0.0	0.0	0.0	0.30561680991847706	3.0846151051545294E-4
46-47	0.0	0.0	0.0	0.3663494539888529	3.770085128522202E-4
48-49	0.0	0.0	0.0	0.43897500296465786	6.169230210309058E-4
50-51	0.0	0.0	0.0	0.5166044831110468	6.169230210309058E-4
52-53	0.0	0.0	0.0	0.5980725953882948	6.169230210309058E-4
54-55	0.0	0.0	0.0	0.684030536318601	6.169230210309058E-4
56-57	0.0	0.0	0.0	0.7676921526706255	6.169230210309058E-4
58-59	0.0	0.0	0.0	0.8664683830379072	6.169230210309058E-4
60-61	0.0	0.0	0.0	0.9730932451727488	6.169230210309058E-4
62-63	0.0	0.0	0.0	1.0873268245669716	6.854700233676731E-4
64-65	0.0	0.0	0.0	1.1994697203899227	6.854700233676731E-4
66-67	0.0	0.0	0.0	1.3203180855096437	6.854700233676731E-4
68-69	0.0	0.0	0.0	1.4555613211200855	6.854700233676731E-4
70-71	0.0	0.0	0.0	1.6052337007224167	7.882905268728241E-4
72-73	0.0	0.0	0.0	1.7472630895641987	0.0011310255385566606
74-75	0.0	0.0	0.0	1.8916916234877674	0.0011652990397250443
76-77	0.0	0.0	0.0	2.057404001636902	0.0011652990397250443
78-79	0.0	0.0	0.0	2.2461824460723596	0.0011652990397250443
80-81	0.0	0.0	0.0	2.431156531878126	0.001268119543230195
82-83	0.0	0.0	0.0	2.631793607717844	0.0013709400467353462
84-85	0.0	0.0	0.0	2.831059743510827	0.0013709400467353462
86-87	0.0	0.0	0.0	3.048422287920716	0.0014394870490721135
88-89	0.0	0.0	0.0	3.2937177357828373	0.0015080340514088807
90-91	0.0	0.0	0.0	3.557726515282897	0.0015080340514088807
92-93	0.0	0.0	0.0	3.8146749535422693	0.0016451280560824154
94-95	6.854700233676731E-5	0.0	0.0	4.07738133999793	0.0017822220607559502
96-97	6.854700233676731E-5	0.0	0.0	4.369391569952558	0.0019535895665978684
98-99	6.854700233676731E-5	0.0	0.0	4.696429318101275	0.0021249570724397866
100-101	6.854700233676731E-5	0.0	0.0	5.043756978941675	0.0021249570724397866
102-103	6.854700233676731E-5	0.0	0.0	5.417646603187572	0.002227777575944938
104-105	6.854700233676731E-5	0.0	0.0	5.810866482092439	0.0023305980794500886
106-107	6.854700233676731E-5	0.0	0.0	6.213168838806926	0.0023305980794500886
108-109	6.854700233676731E-5	0.0	0.0	6.633841792147667	0.002399145081786856
110-111	6.854700233676731E-5	0.0	0.0	7.100304143049368	0.0025705125876287743
112-113	6.854700233676731E-5	0.0	0.0	7.563716152347084	0.002673333091133925
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2435	0.0	50.33867	1
GGTATCA	1455	0.0	50.300884	1
TCAACGC	2975	0.0	40.80155	4
CAACGCA	3020	0.0	40.193584	5
GTACATG	7735	0.0	38.309147	1
AACGCAG	3185	0.0	38.110035	6
TACATGG	8025	0.0	36.70233	2
ATCAACG	3380	0.0	36.264694	3
ACATGGG	8100	0.0	35.921734	3
TATCAAC	3485	0.0	35.00133	2
CATGGGG	7305	0.0	31.93003	4
ATGGGGG	4565	0.0	31.021988	5
ACGCAGA	4110	0.0	29.528908	7
GTGGTAT	785	0.0	28.045652	1
CGCAGAG	4380	0.0	27.844461	8
TGGGGGG	3310	0.0	25.705534	6
GAGTACA	5385	0.0	23.756708	1
GCAGAGT	5150	0.0	22.872677	9
AGTACAT	5405	0.0	22.567926	2
AGAGTAC	4500	0.0	22.478634	5
>>END_MODULE