FastQCFastQC Report
Mon 15 Aug 2016
SRR2921299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458853
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.12434426223889589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.10789298167807175No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.066.969131
GTATCAA32950.056.220621
GTACATG75050.040.2096251
TCAACGC43600.039.6443144
TATCAAC46500.038.9663772
TACATGG78550.038.5466732
CAACGCA45250.038.0669985
ACATGGG80950.037.330213
AACGCAG46600.036.5754626
ATCAACG48400.036.328383
CATGGGG71750.034.7235224
ATGGGGG43650.033.4542475
GTGGTAT9150.029.3887251
ACGCAGA58650.029.0548277
TGGGGGG32150.027.6194526
CGCAGAG61950.027.2120368
TGGTATC9200.027.2101442
GTAAGTA7150.024.1747132
AGAGTAC61850.023.93248210-11
GCAGAGT68750.023.1334069
Produced by FastQC (version 0.11.5)