##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921299_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1458853
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.32609796874668	33.0	30.0	34.0	20.0	34.0
2	30.083248963397956	33.0	31.0	34.0	26.0	34.0
3	30.32282759126519	33.0	31.0	34.0	27.0	34.0
4	32.54132184668366	37.0	35.0	37.0	19.0	37.0
5	32.17584225415446	37.0	33.0	37.0	19.0	37.0
6	32.315069441540714	37.0	35.0	37.0	17.0	37.0
7	32.52009009817987	37.0	35.0	37.0	17.0	37.0
8	32.775722433994375	37.0	35.0	37.0	17.0	37.0
9	34.53459258746426	39.0	37.0	39.0	18.0	39.0
10-11	34.722338714044525	39.0	37.0	39.0	25.5	39.0
12-13	34.731272102124066	39.0	37.0	39.0	25.5	39.0
14-15	36.13688150896629	40.5	38.0	41.0	27.0	41.0
16-17	36.15590672946486	41.0	38.0	41.0	27.0	41.0
18-19	36.19055963829118	41.0	38.0	41.0	27.0	41.0
20-21	36.186474579686916	41.0	38.0	41.0	27.0	41.0
22-23	36.136333818417626	41.0	38.0	41.0	26.5	41.0
24-25	36.08943533035885	41.0	38.0	41.0	26.0	41.0
26-27	35.996881796863704	41.0	38.0	41.0	25.0	41.0
28-29	35.91899286631347	40.0	38.0	41.0	25.0	41.0
30-31	35.826263509757325	40.0	37.0	41.0	24.5	41.0
32-33	35.70255879105023	40.0	37.0	41.0	24.0	41.0
34-35	35.62137789071277	40.0	37.0	41.0	24.0	41.0
36-37	35.542794579028865	40.0	37.0	41.0	23.0	41.0
38-39	35.45055464806941	40.0	36.5	41.0	22.0	41.0
40-41	35.3219008357936	40.0	36.0	41.0	20.5	41.0
42-43	35.181375710918104	40.0	36.0	41.0	18.0	41.0
44-45	35.03210193213435	40.0	35.0	41.0	15.5	41.0
46-47	34.8145964672246	40.0	35.0	41.0	11.0	41.0
48-49	34.59810995350457	40.0	35.0	41.0	7.5	41.0
50-51	34.41196919771903	40.0	35.0	41.0	6.0	41.0
52-53	34.226309299154885	39.0	35.0	41.0	2.0	41.0
54-55	34.01726356253852	39.0	35.0	41.0	2.0	41.0
56-57	33.766879185222905	39.0	35.0	41.0	2.0	41.0
58-59	33.69254852956398	38.5	35.0	41.0	2.0	41.0
60-61	33.44378768799872	38.0	34.5	41.0	2.0	41.0
62-63	33.14809785495866	37.0	34.0	40.0	2.0	41.0
64-65	32.84187954509467	37.0	34.0	40.0	2.0	41.0
66-67	32.51734170612118	36.0	34.0	39.0	2.0	41.0
68-69	32.179132510266626	35.5	34.0	39.0	2.0	41.0
70-71	31.84501111489643	35.0	34.0	38.5	2.0	40.5
72-73	31.50020289912692	35.0	33.0	37.0	2.0	39.5
74-75	31.17323198430548	35.0	33.0	37.0	2.0	39.0
76-77	30.886380259011702	35.0	33.0	36.0	2.0	39.0
78-79	30.634651674980276	35.0	33.0	36.0	2.0	37.0
80-81	30.414765229944347	35.0	33.0	35.5	2.0	37.0
82-83	30.221390366267197	35.0	33.0	35.0	2.0	36.5
84-85	30.058341724628868	35.0	32.5	35.0	2.0	36.0
86-87	29.92237257626368	35.0	32.0	35.0	2.0	36.0
88-89	29.808604431015326	35.0	32.0	35.0	2.0	36.0
90-91	29.712774350808477	35.0	32.0	35.0	2.0	35.0
92-93	29.644824392862063	35.0	32.0	35.0	2.0	35.0
94-95	29.5793986782767	35.0	32.0	35.0	2.0	35.0
96-97	29.511786657051807	35.0	32.0	35.0	2.0	35.0
98-99	29.45980849338487	35.0	32.0	35.0	2.0	35.0
100-101	29.406468300781505	35.0	32.0	35.0	2.0	35.0
102-103	28.74196714816366	34.0	30.0	35.0	2.0	35.0
104-105	29.28779219016584	35.0	31.5	35.0	2.0	35.0
106-107	29.34813514452793	35.0	32.0	35.0	2.0	35.0
108-109	29.41728159040013	35.0	32.0	35.0	2.0	35.0
110-111	29.52623670787941	35.0	32.5	35.0	2.0	35.0
112-113	29.489968488943028	35.0	32.5	35.0	2.0	35.0
114-115	29.428540092798933	35.0	32.0	35.0	2.0	35.0
116-117	29.371444209937533	35.0	32.0	35.0	2.0	35.0
118-119	29.299959625815625	35.0	32.0	35.0	2.0	35.0
120-121	29.207390326509937	35.0	32.0	35.0	2.0	35.0
122-123	29.09900894744022	35.0	32.0	35.0	2.0	35.0
124-125	28.57939833554169	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94018.0
3	6254.0
4	4532.0
5	4202.0
6	4367.0
7	4639.0
8	4876.0
9	4807.0
10	4963.0
11	4922.0
12	5652.0
13	6688.0
14	8069.0
15	6884.0
16	4500.0
17	4067.0
18	3628.0
19	3298.0
20	3153.0
21	3342.0
22	3669.0
23	4577.0
24	6093.0
25	7843.0
26	9100.0
27	10805.0
28	12635.0
29	15359.0
30	18658.0
31	22774.0
32	29271.0
33	39847.0
34	61257.0
35	105125.0
36	241619.0
37	513289.0
38	169829.0
39	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32367214882717	17.9525396030615	11.971844548010631	26.751943700100693
2	16.02539803530582	19.16087501619423	38.13187483591561	26.681852112584338
3	19.280695176278897	22.702767173937332	28.324032647566273	29.692505002217494
4	13.4283210862116	15.248112197255676	31.420620414185365	39.90294630234736
5	14.290197209404527	37.5439463854098	32.06403812348934	16.101818281696332
6	35.03930257980186	33.31447412997351	16.13936334072614	15.506859949498484
7	30.63695258833765	31.25127269162022	18.272183572010864	19.839591148031264
8	28.225643359984755	35.00204633000825	17.809172357200783	18.963137952806214
9	27.812133888058266	15.932643286231071	17.684839186668505	38.57038363904216
10-11	27.174905137143707	25.1273206739847	25.27816212662799	22.41961206224361
12-13	26.57319731306472	22.273284691958537	26.29614312031813	24.85737487465861
14-15	24.077134570789518	23.57471931716218	23.565122736835033	28.78302337521327
16-17	24.361775997992943	26.60370852992042	24.279896603701676	24.754618868384956
18-19	24.40252719088215	25.74628835119097	26.0050190115111	23.846165446415778
20-21	25.1374538764358	24.813637837396914	25.951312435180242	24.09759585098704
22-23	25.0295951682589	24.170015758955838	25.788204843119907	25.012184229665362
24-25	24.67421323464393	24.589489139755685	25.666019811454614	25.07027781414577
26-27	24.251518144734256	24.766203311779872	26.00923465215481	24.973043891331066
28-29	24.34570172594497	25.20476703273051	25.801674329079077	24.64785691224544
30-31	24.99206568447952	24.95878611484502	25.63544784841242	24.413700352263046
32-33	24.589523413256853	25.05622567866673	25.69446681742437	24.65978409065204
34-35	24.680245370849565	25.129639518169412	25.944697649454746	24.24541746152628
36-37	24.72778271697011	24.993847906540275	25.929137479924297	24.349231896565314
38-39	24.626573068019873	25.243427542048448	25.803250910132824	24.32674847979886
40-41	24.61714785519857	25.216317202624257	25.761231597700384	24.405303344476792
42-43	24.422817103573834	25.218990535715385	25.57008828168431	24.788104079026468
44-45	24.284009423841884	25.239006260397723	25.869947143406495	24.607037172353895
46-47	24.32174454862827	25.216831305141778	25.482416665695585	24.979007480534364
48-49	24.36273565602566	25.464800086095035	25.28482993145985	24.887634326419455
50-51	24.25813293045975	25.610325372055993	25.443824703379985	24.68771699410427
52-53	24.57941273041218	25.455169232266716	25.375997444567755	24.589420592753346
54-55	24.121758669310754	25.196370024944255	25.697105877014337	24.984765428730654
56-57	24.22211148073178	25.40811171516253	25.36081428355016	25.008962520555535
58-59	24.342342922830472	25.391009238079505	25.57101366621586	24.695634172874172
60-61	24.674281781646265	25.3303108675103	25.631643489782725	24.36376386106071
62-63	24.420383684990878	25.37414667550466	25.600283236213656	24.605186403290805
64-65	24.230302847511023	25.13255276576872	25.588493151811733	25.04865123490852
66-67	24.205283191658104	25.376168812073598	25.459042137898745	24.959505858369553
68-69	24.005468679846427	25.272560018041574	25.635379301410083	25.086592000701923
70-71	24.086251322100306	25.186190795097247	25.675410750774752	25.052147132027695
72-73	24.192979004738653	25.561725547399224	25.215494638596216	25.02980080926591
74-75	24.01962363582897	25.536774438548644	25.44882863455057	24.99477329107182
76-77	24.02126876388505	25.046114995822062	25.483102135718948	25.449514104573932
78-79	23.891577835463888	25.156338575579586	25.687646390691864	25.264437198264666
80-81	24.15575798246979	25.443173506857786	25.22937540656941	25.171693104103017
82-83	24.23064558252271	25.425111371742048	25.34576821653724	24.998474829198006
84-85	24.0881020911634	25.530193926324312	25.170630625566798	25.21107335694549
86-87	23.779263572135097	25.72959715612197	25.35169753223937	25.13944173950357
88-89	23.703930416566987	25.38597103340775	25.41143624477586	25.498662305249397
90-91	23.978827151121482	25.07637011220143	25.57635314677765	25.368449589899438
92-93	24.07399410218762	25.264318509287477	25.47924866641167	25.182438722113233
94-95	24.11067058207412	25.215820384795716	25.28803470125825	25.38547433187191
96-97	23.746672456629263	25.086703775894176	25.48420961355427	25.682414153922295
98-99	23.574479402654003	25.20658352829243	25.34275214843442	25.876184920619146
100-101	23.648544438678883	25.239794550924593	25.50010179229847	25.61155921809805
102-103	23.470287959102116	25.108938323463708	25.56700366657915	25.85377005085502
104-105	23.608135980801357	25.316498646539436	25.324038816796484	25.751326555862725
106-107	23.660683531314554	25.079848826181983	25.494181891838814	25.765285750664653
108-109	23.586570197656364	25.012595580750524	25.47275413083638	25.928080090756726
110-111	23.42184498379714	25.43689453489827	25.497627407936747	25.643633073367845
112-113	23.440112308486405	25.418911888152152	25.49218933686308	25.648786466498358
114-115	23.401504679889655	25.509914154896823	25.368192790409694	25.720388374803825
116-117	23.183567234685878	25.34137533104179	25.45026310260573	26.0247943316666
118-119	23.238259490276064	25.13640038701757	25.513581430833977	26.11175869187239
120-121	23.17591035684933	25.159903238918847	25.707525991345243	25.956660412886578
122-123	23.07886088049418	25.28796222448355	25.430645206067748	26.20253168895453
124-125	22.850870804279392	25.228527215739767	25.3288115145294	26.591790465451442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	35.5
2	52.5
3	68.0
4	59.5
5	71.5
6	166.0
7	398.5
8	622.0
9	714.5
10	633.5
11	525.0
12	555.0
13	607.5
14	650.5
15	725.5
16	818.0
17	850.5
18	877.5
19	962.5
20	1042.5
21	1097.0
22	1170.5
23	1289.5
24	1439.0
25	1655.5
26	2025.0
27	2529.5
28	3102.0
29	3917.0
30	5103.5
31	6532.0
32	8282.0
33	10182.5
34	12226.0
35	14460.0
36	17333.5
37	20531.0
38	23563.5
39	26790.5
40	29927.0
41	32982.0
42	36160.5
43	40086.5
44	44410.0
45	48481.0
46	52492.5
47	56550.0
48	61159.0
49	65103.5
50	67442.0
51	69276.5
52	69958.5
53	69668.0
54	68928.0
55	67306.5
56	64105.5
57	58508.0
58	53159.5
59	47465.0
60	40536.0
61	33898.5
62	27411.5
63	20886.5
64	15000.5
65	10935.5
66	8115.0
67	6060.5
68	4522.0
69	3329.5
70	2537.0
71	1939.0
72	1456.5
73	1159.0
74	872.5
75	626.5
76	457.0
77	320.0
78	236.5
79	185.5
80	154.5
81	134.0
82	120.5
83	106.5
84	103.0
85	102.0
86	93.5
87	87.5
88	76.0
89	77.0
90	74.5
91	65.0
92	63.0
93	56.5
94	55.0
95	48.5
96	41.0
97	32.5
98	16.0
99	5.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.337593986508579
2	0.0
3	0.0
4	0.01912461365195808
5	0.1736295569190316
6	0.046543414586665
7	0.022620510771133213
8	0.009870768336494492
9	0.007677264261717939
10-11	0.002501965585292007
12-13	0.0572367469512007
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.3991450817868558E-4
92-93	4.7982901635737116E-4
94-95	6.854700233676731E-5
96-97	4.455555151889875E-4
98-99	0.0
100-101	0.0
102-103	0.0
104-105	0.0
106-107	1.7136750584191828E-4
108-109	5.483760186941385E-4
110-111	3.7700851285222024E-4
112-113	9.596580327147423E-4
114-115	3.084615105154529E-4
116-117	7.88290526872824E-4
118-119	3.084615105154529E-4
120-121	1.3709400467353463E-4
122-123	0.001679401557250799
124-125	3.427350116838366E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1458853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.608263895278306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.25565540612067	51.49883780545479
2	9.381469160323478	11.747149938298946
3	3.1723823700720155	5.958520578065915
4	1.5335148431486851	3.840428079487168
5	0.8821633401355089	2.761535759897205
6	0.5720785003285132	2.1490105030429563
7	0.40159933847977336	1.760040615459759
8	0.29380830241118244	1.4715862185586435
9	0.21434365303396186	1.207771559408543
>10	1.2224479187548805	13.486521820884597
>50	0.05573004777904734	2.333625099219831
>100	0.014149061028031524	1.3004612807618008
>500	2.193527947587937E-4	0.08597044335426285
>1k	4.387055895175874E-4	0.3985402981055444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1814	0.12434426223889589	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1574	0.10789298167807175	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.854700233676731E-5	0.0
5	0.0	0.0	0.0	1.3709400467353463E-4	0.0
6	0.0	0.0	0.0	1.3709400467353463E-4	6.854700233676731E-5
7	0.0	0.0	0.0	1.3709400467353463E-4	6.854700233676731E-5
8	0.0	0.0	0.0	1.3709400467353463E-4	1.3709400467353463E-4
9	0.0	0.0	0.0	1.3709400467353463E-4	1.3709400467353463E-4
10-11	0.0	0.0	0.0	1.3709400467353463E-4	1.3709400467353463E-4
12-13	0.0	0.0	0.0	1.3709400467353463E-4	1.3709400467353463E-4
14-15	0.0	0.0	0.0	1.3709400467353463E-4	1.7136750584191828E-4
16-17	0.0	0.0	0.0	2.0564100701030193E-4	2.0564100701030193E-4
18-19	0.0	0.0	0.0	3.770085128522202E-4	2.7418800934706926E-4
20-21	0.0	0.0	0.0	4.7982901635737116E-4	3.770085128522202E-4
22-23	0.0	0.0	0.0	6.854700233676731E-4	5.141025175257548E-4
24-25	0.0	0.0	0.0	9.596580327147423E-4	6.169230210309058E-4
26-27	0.0	0.0	0.0	0.0011310255385566606	6.169230210309058E-4
28-29	0.0	0.0	0.0	0.002776153594639076	6.169230210309058E-4
30-31	0.0	0.0	0.0	0.009802221334157726	6.169230210309058E-4
32-33	0.0	0.0	0.0	0.024882561848246533	6.169230210309058E-4
34-35	0.0	0.0	0.0	0.043253158474500175	6.169230210309058E-4
36-37	0.0	0.0	0.0	0.07080905341388063	6.854700233676731E-4
38-39	0.0	0.0	0.0	0.11916896356246998	6.854700233676731E-4
40-41	0.0	0.0	0.0	0.1778109240615744	6.854700233676731E-4
42-43	0.0	0.0	0.0	0.22952963732466536	6.854700233676731E-4
44-45	0.0	0.0	0.0	0.2872804867933918	7.197435245360567E-4
46-47	0.0	0.0	0.0	0.3428378321873417	8.225640280412077E-4
48-49	0.0	0.0	0.0	0.4110078260112568	8.911110303779751E-4
50-51	0.0	0.0	0.0	0.4834620074812198	8.911110303779751E-4
52-53	0.0	0.0	0.0	0.5586237955434852	9.93931533883126E-4
54-55	0.0	0.0	0.0	0.6402290018254067	0.0011652990397250443
56-57	0.0	0.0	0.0	0.7202233535524141	0.0011652990397250443
58-59	0.0	0.0	0.0	0.8133101827257441	0.001199572540893428
60-61	0.0	0.0	0.0	0.9129775241234037	0.0013023930443985788
62-63	0.0	0.0	0.0	1.0218644373353587	0.0013023930443985788
64-65	0.0	0.0	0.0	1.127940923451506	0.0013366665455669625
66-67	0.0	0.0	0.0	1.2415918533258663	0.00140521354790373
68-69	0.0	0.0	0.0	1.3681981666418754	0.0014394870490721135
70-71	0.0	0.0	0.0	1.5078969574042071	0.0014394870490721135
72-73	0.0	0.0	0.0	1.6403983129211785	0.001679401557250799
74-75	0.0	0.0	0.0	1.776395565557325	0.0017822220607559502
76-77	0.0	0.0	0.0	1.9332653804050168	0.0018507690630927174
78-79	0.0	0.0	0.0	2.1098767319256977	0.0018507690630927174
80-81	0.0	0.0	0.0	2.2834034683412243	0.0018507690630927174
82-83	0.0	0.0	0.0	2.4717363572614923	0.0019193160654294847
84-85	0.0	0.0	0.0	2.6583555711233413	0.0019193160654294847
86-87	0.0	0.0	0.0	2.863551022618454	0.0020564100701030196
88-89	0.0	0.0	0.0	3.0958568135377584	0.002193504074776554
90-91	0.0	0.0	0.0	3.346019098565791	0.002193504074776554
92-93	0.0	0.0	0.0	3.588812580842621	0.002262051077113321
94-95	0.0	0.0	0.0	3.835376148247973	0.002399145081786856
96-97	0.0	0.0	0.0	4.107027918508582	0.0024334185829552393
98-99	0.0	0.0	0.0	4.418608317630357	0.002467692084123623
100-101	0.0	0.0	0.0	4.745954527289589	0.002467692084123623
102-103	0.0	0.0	0.0	5.10027398236834	0.002673333091133925
104-105	0.0	0.0	0.0	5.47347813659087	0.0028104270958074595
106-107	0.0	0.0	0.0	5.853399897042403	0.0028447005969758432
108-109	0.0	0.0	0.0	6.253474476180945	0.002878974098144227
110-111	0.0	0.0	0.0	6.695876829262441	0.0029132475993126107
112-113	0.0	0.0	0.0	7.137490891817064	0.0029475211004809944
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1740	0.0	66.96913	1
GTATCAA	3295	0.0	56.22062	1
GTACATG	7505	0.0	40.209625	1
TCAACGC	4360	0.0	39.644314	4
TATCAAC	4650	0.0	38.966377	2
TACATGG	7855	0.0	38.546673	2
CAACGCA	4525	0.0	38.066998	5
ACATGGG	8095	0.0	37.33021	3
AACGCAG	4660	0.0	36.575462	6
ATCAACG	4840	0.0	36.32838	3
CATGGGG	7175	0.0	34.723522	4
ATGGGGG	4365	0.0	33.454247	5
GTGGTAT	915	0.0	29.388725	1
ACGCAGA	5865	0.0	29.054827	7
TGGGGGG	3215	0.0	27.619452	6
CGCAGAG	6195	0.0	27.212036	8
TGGTATC	920	0.0	27.210144	2
GTAAGTA	715	0.0	24.174713	2
AGAGTAC	6185	0.0	23.932482	10-11
GCAGAGT	6875	0.0	23.133406	9
>>END_MODULE