FastQCFastQC Report
Mon 15 Aug 2016
SRR2921298_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921298_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648453
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.059.513261
GTATCAA23850.055.3959921
TCAACGC29150.043.688044
CAACGCA29700.042.8790055
AACGCAG30400.041.8878446
GTACATG75200.040.4405251
TATCAAC32550.039.67312
ATCAACG33000.038.5911063
TACATGG77900.038.5781442
ACATGGG79000.037.513683
CATGGGG73700.033.5095024
ATGGGGG46700.033.131715
ACGCAGA39900.031.4604687
CGCAGAG42400.029.3248678
TGGGGGG34000.026.6018926
GCAGAGT49700.024.297919
AGTACAT50950.022.5424182
GAGTACA52000.022.4319211
AGAGTAC44950.021.43949710-11
CAGAGTA46800.021.2275510-11
Produced by FastQC (version 0.11.5)