##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921298_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1648453 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.377235505046247 31.0 31.0 34.0 27.0 34.0 2 30.4631063184695 31.0 31.0 34.0 26.0 34.0 3 30.699276230502175 33.0 31.0 34.0 27.0 34.0 4 34.05829647554404 37.0 35.0 37.0 30.0 37.0 5 34.139705529972645 37.0 35.0 37.0 30.0 37.0 6 34.206650113773335 37.0 35.0 37.0 31.0 37.0 7 34.13041257469882 37.0 35.0 37.0 30.0 37.0 8 34.13660262076019 37.0 35.0 37.0 30.0 37.0 9 35.75389774534063 39.0 37.0 39.0 30.0 39.0 10-11 35.74850723678503 39.0 37.0 39.0 30.0 39.0 12-13 35.67416025813293 39.0 37.0 39.0 30.0 39.0 14-15 37.00234704902111 41.0 38.0 41.0 30.0 41.0 16-17 36.98351424032108 41.0 38.0 41.0 30.0 41.0 18-19 36.97996576183853 41.0 38.0 41.0 30.0 41.0 20-21 37.008285950524524 41.0 38.0 41.0 30.0 41.0 22-23 36.955820093141874 41.0 38.0 41.0 30.0 41.0 24-25 36.94265562924754 41.0 38.0 41.0 30.0 41.0 26-27 36.90370729405085 41.0 38.0 41.0 30.0 41.0 28-29 36.83330492285798 40.0 38.0 41.0 30.0 41.0 30-31 36.75282067489944 40.0 38.0 41.0 30.0 41.0 32-33 36.66947556284589 40.0 38.0 41.0 30.0 41.0 34-35 36.564154088712264 40.0 38.0 41.0 29.5 41.0 36-37 36.4552507714809 40.0 37.0 41.0 29.0 41.0 38-39 36.35486756370973 40.0 37.0 41.0 28.5 41.0 40-41 36.252876484801206 40.0 37.0 41.0 28.0 41.0 42-43 36.06915665778763 40.0 36.5 41.0 27.5 41.0 44-45 35.839083977523174 40.0 36.0 41.0 27.0 41.0 46-47 35.626338755184406 40.0 35.5 41.0 26.0 41.0 48-49 35.424838924737315 40.0 35.0 41.0 26.0 41.0 50-51 35.255746751651394 39.5 35.0 41.0 26.0 41.0 52-53 35.06410525504822 39.0 35.0 41.0 25.0 41.0 54-55 34.85443625022976 39.0 35.0 41.0 24.0 41.0 56-57 34.61051118836873 38.5 35.0 40.5 24.0 41.0 58-59 34.34787252047829 38.0 34.0 40.0 23.0 41.0 60-61 34.061222552296 38.0 34.0 40.0 22.5 41.0 62-63 33.74195594293559 37.0 34.0 40.0 22.0 41.0 64-65 33.39116583851647 36.5 34.0 39.5 21.5 41.0 66-67 33.0465497044805 36.0 33.0 39.0 20.5 41.0 68-69 32.69598799601808 35.5 33.0 39.0 20.0 40.0 70-71 32.635366916739514 35.0 33.0 38.5 20.0 40.0 72-73 32.345008319921774 35.0 33.0 37.5 19.5 40.0 74-75 32.02380170984553 35.0 33.0 37.0 18.5 39.0 76-77 31.726462628901157 35.0 33.0 36.5 18.0 39.0 78-79 31.443069653790552 35.0 33.0 36.0 17.5 38.0 80-81 31.171743446734602 35.0 33.0 36.0 14.5 37.0 82-83 30.948006403579598 35.0 32.5 35.0 12.0 37.0 84-85 30.737688002023717 35.0 32.0 35.0 9.5 36.0 86-87 30.57626817385755 35.0 32.0 35.0 7.5 36.0 88-89 30.4393076417708 35.0 32.0 35.0 7.0 36.0 90-91 30.31475510675767 35.0 32.0 35.0 6.0 35.5 92-93 30.208758150823833 35.0 32.0 35.0 2.0 35.0 94-95 30.092794881018747 35.0 32.0 35.0 2.0 35.0 96-97 30.007671434975702 35.0 32.0 35.0 2.0 35.0 98-99 29.927926971530276 35.0 31.5 35.0 2.0 35.0 100-101 29.841236298517458 35.0 31.0 35.0 2.0 35.0 102-103 29.018823405944847 33.5 29.5 35.0 2.0 35.0 104-105 29.61434083956291 34.0 31.0 35.0 2.0 35.0 106-107 29.69447657895008 35.0 31.0 35.0 2.0 35.0 108-109 29.638736136244106 35.0 31.0 35.0 2.0 35.0 110-111 29.567287329393075 35.0 31.0 35.0 2.0 35.0 112-113 29.484360184973426 35.0 31.0 35.0 2.0 35.0 114-115 29.363531747644608 34.0 31.0 35.0 2.0 35.0 116-117 29.235710693601817 34.0 31.0 35.0 2.0 35.0 118-119 29.235229029884383 34.5 31.0 35.0 2.0 35.0 120-121 29.40701221084253 35.0 31.5 35.0 2.0 35.0 122-123 29.284647787956345 35.0 32.0 35.0 2.0 35.0 124-125 28.72773897708943 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58535.0 3 8706.0 4 5349.0 5 4513.0 6 4487.0 7 5106.0 8 5448.0 9 5866.0 10 6414.0 11 6900.0 12 7108.0 13 7069.0 14 7127.0 15 6149.0 16 4987.0 17 4512.0 18 4485.0 19 4346.0 20 4286.0 21 4478.0 22 5063.0 23 6681.0 24 9649.0 25 11759.0 26 12753.0 27 14273.0 28 17423.0 29 20559.0 30 25035.0 31 31203.0 32 40638.0 33 55373.0 34 85539.0 35 143244.0 36 304874.0 37 539718.0 38 158717.0 39 81.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.28138759269922 17.753109894570283 12.103152091939908 24.862350420790595 2 16.230737496056495 18.9053437522751 39.130851554347565 25.733067197320842 3 19.735954415401306 23.578454093853214 28.283794704284965 28.40179678646052 4 13.536496357007778 14.648778419070604 35.11566629232639 36.69905893159523 5 14.535128861742008 37.59404289344829 33.10679923825332 14.764029006556385 6 34.567934197232326 33.96524783747129 16.65561256223623 14.811205403060157 7 30.159345532998188 31.602580203635146 19.59087318488426 18.647201078482407 8 27.498537837844395 35.395426890678806 18.828765643921653 18.277269627555143 9 27.65838562448773 15.673817253259866 18.286140351622244 38.38165677063016 10-11 26.67338867462994 25.368967255691715 26.536825137397724 21.42081893228062 12-13 26.216407071850554 22.643474821304853 26.948144130633402 24.19197397621119 14-15 23.70861614173706 24.11338503762555 23.878149516970932 28.29984930366646 16-17 24.321028697336043 27.170408935825318 24.621263595199153 23.887298771639482 18-19 24.300520853713543 26.61989872912422 25.835872991895236 23.243707425267 20-21 25.039607537307525 25.59843553640117 25.681942754634075 23.68001417165723 22-23 24.99421395124795 25.075203467200613 25.59760101167129 24.33298156988015 24-25 24.52142308325808 25.356803665239884 25.60405083748875 24.51772241401328 26-27 24.222400849321303 25.57376203837112 25.73455676044589 24.469280351861684 28-29 24.32498346071841 26.023835372192227 25.578510854955734 24.072670312133628 30-31 24.87686992847733 25.62282416112757 25.502977659289527 23.99732825110557 32-33 24.506565027672156 25.586241728307808 25.546959247839084 24.360233996180956 34-35 24.499383917797466 25.765097654034204 25.67197247620151 24.063545951966823 36-37 24.56286857346353 25.765690942628954 25.625913234079434 24.045527249828083 38-39 24.56672147960349 25.859239043555206 25.539097518797014 24.034941958044293 40-41 24.531373610106726 25.674714013230375 25.618899879150263 24.17501249751263 42-43 24.296856728671827 25.697334363466553 25.534442273561037 24.471366634300583 44-45 24.178841886700305 25.67851352355645 25.772093227832453 24.370551361910785 46-47 24.210327705394878 25.61086626206664 25.657975562012737 24.520830470525745 48-49 24.120200289325677 25.793271979787853 25.656404978294507 24.430122752591966 50-51 24.20016640797496 25.865671818357477 25.6544313630803 24.27973041058726 52-53 24.346758620857013 25.85844571291292 25.556205360853262 24.23859030537681 54-55 24.083780750447417 25.51839718506385 25.818545818545818 24.579276245942914 56-57 24.234367402332 25.706648531101088 25.520732694645144 24.538251371921767 58-59 24.262528104134233 25.728584134040794 25.719908707822565 24.288979054002404 60-61 24.503522585741123 25.749819669575206 25.732529788644854 24.01412795603881 62-63 24.40473211314678 25.790295716121747 25.657981945024567 24.14699022570691 64-65 24.283741801863997 25.552704967808044 25.640639095167234 24.52291413516073 66-67 24.290774656132708 25.79540070481193 25.515217033905348 24.398607605150016 68-69 24.13235594776087 25.7042375282329 25.735026023103202 24.428380500903028 70-71 24.113746237952498 25.59733215398718 25.873119582909432 24.41580202515089 72-73 24.176554164154098 25.995797080524007 25.460907631623332 24.366741123698567 74-75 24.05886112406147 25.985379053542296 25.57719251256606 24.378567309830178 76-77 24.154356819790152 25.608151783377025 25.595047972989164 24.64244342384366 78-79 23.932305245178735 25.63947635266815 25.80224029429759 24.625978107855527 80-81 24.21015051266395 25.943923181243385 25.425693225459 24.42023308063367 82-83 24.351339726358155 25.86293398464776 25.586143552466424 24.19958273652766 84-85 24.315164171009283 25.96398766848076 25.373499246039778 24.34734891447018 86-87 24.013185434505385 26.11430693051287 25.58907964041268 24.283427994569063 88-89 24.025864315174097 25.713984984574168 25.659264310260156 24.600886389991576 90-91 24.2332186238809 25.613523683280583 25.691904262854003 24.461353429984513 92-93 24.319403657855553 25.77422222869348 25.603623820803723 24.30275029264725 94-95 24.26050310813922 25.798541375273874 25.53033108894529 24.410624427641615 96-97 24.062747034673883 25.66570062142776 25.70340611332518 24.56814623057318 98-99 23.949380496828017 25.72055957003938 25.50883102181168 24.821228911320926 100-101 23.957017911326993 25.856655389490296 25.63740111489646 24.548925584286252 102-103 23.85680589966779 25.74379809313962 25.767124251682272 24.632271755510313 104-105 23.995735180814986 25.838373666106516 25.57074564569848 24.595145507380018 106-107 23.991707981159134 25.74639089697949 25.73134538430365 24.530555737557723 108-109 23.933986840077704 25.749603540050646 25.688633035214863 24.62777658465679 110-111 23.81516012628585 26.074546023718636 25.64908355396148 24.461210296034032 112-113 23.849606927610797 26.10587914476233 25.68018343074831 24.364330496878566 114-115 23.84907553563607 26.224449683149984 25.520500311208227 24.405974470005713 116-117 23.776943905959804 26.091337577647195 25.61311670730705 24.518601809085954 118-119 23.80921712576502 25.942373369480354 25.690227241381614 24.55818226337301 120-121 23.74942212418051 26.00959910888819 25.872122299757688 24.368856467173618 122-123 23.77123170747243 26.204472285262426 25.583695650195732 24.44060035706941 124-125 23.703487335160535 26.167449839907498 25.5443387676214 24.584724057310567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 338.0 1 302.5 2 227.5 3 172.0 4 143.5 5 125.0 6 127.0 7 162.5 8 208.5 9 249.0 10 259.0 11 250.5 12 298.5 13 367.5 14 375.0 15 394.5 16 444.5 17 492.0 18 561.5 19 652.5 20 706.5 21 753.0 22 906.5 23 1098.5 24 1314.0 25 1715.0 26 2428.5 27 3429.0 28 4434.5 29 5832.5 30 7813.0 31 9911.5 32 12505.0 33 15460.5 34 18512.5 35 21667.5 36 25296.0 37 28812.5 38 32128.0 39 35752.5 40 39001.5 41 41707.5 42 44670.5 43 48852.0 44 53644.0 45 58109.0 46 61368.0 47 64786.5 48 69297.0 49 72611.5 50 73882.5 51 74481.0 52 73917.5 53 72401.0 54 71798.0 55 69513.5 56 64946.5 57 59408.5 58 53908.0 59 48343.0 60 41424.5 61 34742.5 62 28358.5 63 21955.5 64 16477.0 65 12606.5 66 9670.0 67 7329.0 68 5628.5 69 4306.5 70 3308.5 71 2441.5 72 1849.5 73 1454.5 74 1124.0 75 818.0 76 580.5 77 405.5 78 262.0 79 166.0 80 104.5 81 73.0 82 57.0 83 42.0 84 28.0 85 20.0 86 20.5 87 18.5 88 13.0 89 13.5 90 13.0 91 6.5 92 3.5 93 4.0 94 6.5 95 5.0 96 2.0 97 2.0 98 1.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014923082429405023 2 0.010494688049947435 3 0.01073733979676703 4 0.013345846075077663 5 0.008250159391866192 6 0.030210142479039435 7 0.012799879644733577 8 0.012678553771323781 9 0.00909944050573477 10-11 0.008250159391866192 12-13 0.006976237721063324 14-15 0.00636960835401434 16-17 0.004671046126277182 18-19 0.006157288075547195 20-21 0.005611321645203109 22-23 0.005550658708498211 24-25 0.006217951012252093 26-27 0.004731709062982081 28-29 0.006339276885661891 30-31 0.005732647518612906 32-33 0.008219827923513744 34-35 0.008128833518456396 36-37 0.007370546809645164 38-39 0.007249220936235368 40-41 0.007582867088112309 42-43 0.007431209746350063 44-45 0.007613198556464759 46-47 0.009615075467726407 48-49 0.00834115379692354 50-51 0.005732647518612906 52-53 0.005823641923670253 54-55 0.006248282480604542 56-57 0.007673861493169657 58-59 0.007218889467882918 60-61 0.005459664303440862 62-63 0.0059752992654325 64-65 0.0036397762022939086 66-67 0.004246405569342893 68-69 0.006672923037538831 70-71 0.004974360809801674 72-73 0.004549720252867385 74-75 0.003912759417465952 76-77 0.004701377594629632 78-79 0.003245467113712068 80-81 0.003245467113712068 82-83 0.005702316050260456 84-85 0.00949374959431661 86-87 0.007218889467882918 88-89 0.004944029341449226 90-91 0.00518668108826882 92-93 0.00834115379692354 94-95 0.007249220936235368 96-97 0.009220766379144566 98-99 0.007036900657768222 100-101 0.008401816733628437 102-103 0.005399001366735964 104-105 0.005035023746506573 106-107 0.007340215341292715 108-109 0.006976237721063324 110-111 0.007825518834931903 112-113 0.005611321645203109 114-115 0.0023658545314910405 116-117 0.003124141240302271 118-119 0.15663170257204784 120-121 0.010616013923357233 122-123 0.002062539847966548 124-125 0.0018805510378518526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1648453.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.2253586405475 #Duplication Level Percentage of deduplicated Percentage of total 1 81.41502119089643 52.28908934713098 2 10.327557134855923 13.265821217337336 3 3.222849118256326 6.209659213931545 4 1.5637157431826019 4.017208176710915 5 0.8700287321635117 2.793895367539119 6 0.5678194390197514 2.1881044268470813 7 0.40062363722603483 1.801113774650588 8 0.2934727302451355 1.5078713080971584 9 0.22419595352878322 1.2959158969031062 >10 1.0670726405790414 11.925335957465219 >50 0.037907313539527025 1.6375018266301717 >100 0.009452847822615905 0.9432656501413906 >500 2.8351868433994335E-4 0.12521783661529637 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2132587340979694E-4 0.0 0.0 0.0 6.066293670489847E-5 7 1.2132587340979694E-4 0.0 0.0 6.066293670489847E-5 6.066293670489847E-5 8 1.2132587340979694E-4 0.0 0.0 6.066293670489847E-5 6.066293670489847E-5 9 1.2132587340979694E-4 0.0 0.0 6.066293670489847E-5 6.066293670489847E-5 10-11 1.2132587340979694E-4 0.0 0.0 1.2132587340979694E-4 6.066293670489847E-5 12-13 1.2132587340979694E-4 0.0 0.0 1.2132587340979694E-4 6.066293670489847E-5 14-15 1.2132587340979694E-4 0.0 0.0 1.2132587340979694E-4 1.516573417622462E-4 16-17 1.2132587340979694E-4 0.0 0.0 1.516573417622462E-4 2.4265174681959388E-4 18-19 1.2132587340979694E-4 0.0 0.0 1.819888101146954E-4 2.4265174681959388E-4 20-21 1.2132587340979694E-4 0.0 0.0 1.819888101146954E-4 2.4265174681959388E-4 22-23 1.2132587340979694E-4 0.0 0.0 2.4265174681959388E-4 2.4265174681959388E-4 24-25 1.2132587340979694E-4 0.0 0.0 4.549720252867385E-4 2.4265174681959388E-4 26-27 1.2132587340979694E-4 0.0 0.0 6.976237721063325E-4 3.033146835244924E-4 28-29 1.2132587340979694E-4 0.0 0.0 0.002578174809958185 3.033146835244924E-4 30-31 1.2132587340979694E-4 0.0 0.0 0.009766732809488653 3.3364615187694163E-4 32-33 1.2132587340979694E-4 0.0 0.0 0.025539096352762258 3.639776202293908E-4 34-35 1.2132587340979694E-4 0.0 0.0 0.044617589946452825 4.8530349363918777E-4 36-37 1.2132587340979694E-4 0.0 0.0 0.07288651845093551 4.8530349363918777E-4 38-39 1.2132587340979694E-4 0.0 0.0 0.12059791816933815 4.8530349363918777E-4 40-41 1.2132587340979694E-4 0.0 0.0 0.1791983150262701 4.8530349363918777E-4 42-43 1.2132587340979694E-4 0.0 0.0 0.23155042940259746 4.8530349363918777E-4 44-45 1.2132587340979694E-4 0.0 0.0 0.2878456346647432 4.8530349363918777E-4 46-47 1.2132587340979694E-4 0.0 0.0 0.3472346496988389 4.8530349363918777E-4 48-49 1.2132587340979694E-4 0.0 0.0 0.4166330492892427 4.8530349363918777E-4 50-51 1.2132587340979694E-4 0.0 0.0 0.4907328264742762 4.8530349363918777E-4 52-53 1.2132587340979694E-4 0.0 0.0 0.5659245365199979 6.066293670489848E-4 54-55 1.2132587340979694E-4 0.0 0.0 0.64979104651452 7.279552404587816E-4 56-57 1.2132587340979694E-4 0.0 0.0 0.7355987704835989 7.279552404587816E-4 58-59 1.2132587340979694E-4 0.0 0.0 0.8339030594138868 7.582867088112309E-4 60-61 1.2132587340979694E-4 0.0 0.0 0.934118230850379 8.189496455161294E-4 62-63 1.819888101146954E-4 0.0 0.0 1.0454043882355153 8.492811138685786E-4 64-65 1.819888101146954E-4 0.0 0.0 1.152656460329776 8.492811138685786E-4 66-67 1.819888101146954E-4 0.0 0.0 1.2716771421447866 8.492811138685786E-4 68-69 1.819888101146954E-4 0.0 0.0 1.403497703604531 8.492811138685786E-4 70-71 1.819888101146954E-4 0.0 0.0 1.5492100775696973 9.09944050573477E-4 72-73 1.819888101146954E-4 0.0 0.0 1.6835481509026948 0.0010009384556308248 74-75 1.819888101146954E-4 0.0 0.0 1.8206463878557653 0.001031269923983274 76-77 1.819888101146954E-4 0.0 0.0 1.9794012932124847 0.001031269923983274 78-79 1.819888101146954E-4 6.066293670489847E-5 0.0 2.161875406820819 0.001031269923983274 80-81 1.819888101146954E-4 6.066293670489847E-5 0.0 2.3445921721759735 0.001031269923983274 82-83 1.819888101146954E-4 6.066293670489847E-5 0.0 2.5344671640623058 0.001031269923983274 84-85 1.819888101146954E-4 6.066293670489847E-5 0.0 2.7314093880747583 0.001031269923983274 86-87 1.819888101146954E-4 6.066293670489847E-5 0.0 2.950402589579442 0.001031269923983274 88-89 1.819888101146954E-4 6.066293670489847E-5 0.0 3.197634388120256 0.0010616013923357233 90-91 1.819888101146954E-4 6.066293670489847E-5 0.0 3.454026290103509 0.0010919328606881725 92-93 1.819888101146954E-4 6.066293670489847E-5 0.0 3.7120560913777947 0.0010919328606881725 94-95 1.819888101146954E-4 6.066293670489847E-5 0.0 3.9776384282718404 0.001152595797393071 96-97 1.819888101146954E-4 6.066293670489847E-5 0.0 4.269093507670525 0.001152595797393071 98-99 1.819888101146954E-4 6.066293670489847E-5 0.0 4.598341596636361 0.001152595797393071 100-101 1.819888101146954E-4 6.066293670489847E-5 0.0 4.946273870107307 0.001152595797393071 102-103 2.1232027846714463E-4 6.066293670489847E-5 0.0 5.312950991020065 0.001152595797393071 104-105 2.4265174681959388E-4 6.066293670489847E-5 0.0 5.693732244716713 0.001152595797393071 106-107 2.4265174681959388E-4 6.066293670489847E-5 0.0 6.090043210209815 0.0012132587340979695 108-109 2.4265174681959388E-4 9.09944050573477E-5 0.0 6.502854494486649 0.0012739216708028678 110-111 2.4265174681959388E-4 1.2132587340979694E-4 0.0 6.969959107114367 0.001425579012565114 112-113 2.4265174681959388E-4 1.2132587340979694E-4 0.0 7.436730073590208 0.0015165734176224618 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1280 0.0 59.51326 1 GTATCAA 2385 0.0 55.395992 1 TCAACGC 2915 0.0 43.68804 4 CAACGCA 2970 0.0 42.879005 5 AACGCAG 3040 0.0 41.887844 6 GTACATG 7520 0.0 40.440525 1 TATCAAC 3255 0.0 39.6731 2 ATCAACG 3300 0.0 38.591106 3 TACATGG 7790 0.0 38.578144 2 ACATGGG 7900 0.0 37.51368 3 CATGGGG 7370 0.0 33.509502 4 ATGGGGG 4670 0.0 33.13171 5 ACGCAGA 3990 0.0 31.460468 7 CGCAGAG 4240 0.0 29.324867 8 TGGGGGG 3400 0.0 26.601892 6 GCAGAGT 4970 0.0 24.29791 9 AGTACAT 5095 0.0 22.542418 2 GAGTACA 5200 0.0 22.431921 1 AGAGTAC 4495 0.0 21.439497 10-11 CAGAGTA 4680 0.0 21.22755 10-11 >>END_MODULE