FastQCFastQC Report
Mon 15 Aug 2016
SRR2921298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648453
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19230.11665482728351977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.065.832911
GTATCAA31700.050.1316571
GTACATG77400.041.1411061
TACATGG81100.039.2362142
ACATGGG82550.038.113913
CATGGGG74800.036.0083774
TCAACGC42700.035.5859834
TATCAAC44550.035.5795632
CAACGCA44050.034.495385
ATCAACG45600.033.4535183
AACGCAG45600.033.0624926
ATGGGGG47300.031.8732285
TATACCG2153.972673E-927.7158535
TGGGGGG32350.026.1573626
ACGCAGA57600.025.6493957
GTGGTAT9950.024.617871
GTATCGC1250.001619373823.8363576
CGCAGAG61550.023.516548
ATGGGGA27500.022.7521935
AGAGTAC61050.022.5472185
Produced by FastQC (version 0.11.5)