##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1648453
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.187006848238923	31.0	30.0	34.0	19.0	34.0
2	29.964927116514694	33.0	31.0	34.0	26.0	34.0
3	30.201492550894688	33.0	31.0	34.0	26.0	34.0
4	32.37743811925484	37.0	35.0	37.0	19.0	37.0
5	32.01217141162047	37.0	33.0	37.0	19.0	37.0
6	32.15058603430004	37.0	35.0	37.0	17.0	37.0
7	32.358557993464174	37.0	35.0	37.0	17.0	37.0
8	32.61732970245436	37.0	35.0	37.0	17.0	37.0
9	34.36609536334976	39.0	37.0	39.0	17.0	39.0
10-11	34.56194019483722	39.0	37.0	39.0	22.5	39.0
12-13	34.580792415677	39.0	37.0	39.0	24.0	39.0
14-15	35.979902975699034	40.5	38.0	41.0	25.0	41.0
16-17	35.99985774541343	41.0	38.0	41.0	25.0	41.0
18-19	36.03120198149416	41.0	38.0	41.0	25.0	41.0
20-21	36.02489273276217	41.0	38.0	41.0	25.0	41.0
22-23	35.97727323739288	41.0	38.0	41.0	25.0	41.0
24-25	35.93071595004528	41.0	38.0	41.0	25.0	41.0
26-27	35.836219473651965	40.5	38.0	41.0	24.0	41.0
28-29	35.759874864494165	40.0	37.5	41.0	23.5	41.0
30-31	35.66180655438766	40.0	37.0	41.0	23.0	41.0
32-33	35.53931898573997	40.0	37.0	41.0	22.0	41.0
34-35	35.46292827275027	40.0	37.0	41.0	21.0	41.0
36-37	35.37966960538153	40.0	36.5	41.0	19.0	41.0
38-39	35.28271809993976	40.0	36.0	41.0	18.0	41.0
40-41	35.1596848681764	40.0	36.0	41.0	16.0	41.0
42-43	35.02407196322855	40.0	35.5	41.0	15.0	41.0
44-45	34.872601463311355	40.0	35.0	41.0	10.5	41.0
46-47	34.66335072944148	40.0	35.0	41.0	7.5	41.0
48-49	34.457207757819	40.0	35.0	41.0	2.0	41.0
50-51	34.27628995185183	40.0	35.0	41.0	2.0	41.0
52-53	34.09081757259685	39.0	35.0	41.0	2.0	41.0
54-55	33.88813178173718	39.0	35.0	41.0	2.0	41.0
56-57	33.64706819060052	39.0	35.0	41.0	2.0	41.0
58-59	33.58811139899045	39.0	35.0	41.0	2.0	41.0
60-61	33.35063298741305	38.0	34.5	41.0	2.0	41.0
62-63	33.07080426314854	37.0	34.0	40.5	2.0	41.0
64-65	32.77045205413803	37.0	34.0	40.0	2.0	41.0
66-67	32.45447034280019	36.0	34.0	39.0	2.0	41.0
68-69	32.11887751728439	36.0	34.0	39.0	2.0	41.0
70-71	31.791775683019168	35.0	33.5	38.5	2.0	40.5
72-73	31.4529513428651	35.0	33.0	37.0	2.0	39.5
74-75	31.125264717890047	35.0	33.0	37.0	2.0	39.0
76-77	30.821707079304048	35.0	33.0	36.5	2.0	39.0
78-79	30.563226249095365	35.0	33.0	36.0	2.0	38.0
80-81	30.33386029204351	35.0	33.0	36.0	2.0	37.0
82-83	30.1308529876193	35.0	32.0	35.0	2.0	37.0
84-85	29.954275311458684	35.0	32.0	35.0	2.0	36.0
86-87	29.807796764602934	35.0	32.0	35.0	2.0	36.0
88-89	29.68389878267685	35.0	32.0	35.0	2.0	36.0
90-91	29.579765392158585	35.0	32.0	35.0	2.0	35.5
92-93	29.508042692148337	35.0	32.0	35.0	2.0	35.0
94-95	29.435890801860893	35.0	32.0	35.0	2.0	35.0
96-97	29.372769196331348	35.0	32.0	35.0	2.0	35.0
98-99	29.31579911589836	35.0	32.0	35.0	2.0	35.0
100-101	29.262818230183086	35.0	31.5	35.0	2.0	35.0
102-103	28.594399415694593	34.0	30.0	35.0	2.0	35.0
104-105	29.134136672383136	35.0	31.0	35.0	2.0	35.0
106-107	29.194313092335662	35.0	31.0	35.0	2.0	35.0
108-109	29.263805216163277	35.0	32.0	35.0	2.0	35.0
110-111	29.364901213440724	35.0	32.0	35.0	2.0	35.0
112-113	29.333178440634946	35.0	32.0	35.0	2.0	35.0
114-115	29.274800980070403	35.0	32.0	35.0	2.0	35.0
116-117	29.21312739884	35.0	32.0	35.0	2.0	35.0
118-119	29.139516868239497	35.0	32.0	35.0	2.0	35.0
120-121	29.04863620618847	35.0	32.0	35.0	2.0	35.0
122-123	28.93828698786074	35.0	32.0	35.0	2.0	35.0
124-125	28.42144028370842	34.5	30.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	112065.0
3	6896.0
4	5219.0
5	4980.0
6	5095.0
7	5354.0
8	5612.0
9	5547.0
10	5892.0
11	5941.0
12	6471.0
13	7517.0
14	8974.0
15	7782.0
16	5160.0
17	4537.0
18	4073.0
19	3868.0
20	3611.0
21	3684.0
22	4214.0
23	5209.0
24	6792.0
25	9021.0
26	10683.0
27	12279.0
28	14724.0
29	17176.0
30	20896.0
31	26126.0
32	33319.0
33	44977.0
34	68023.0
35	116764.0
36	263642.0
37	566220.0
38	209773.0
39	337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02541172002064	18.148854683041325	11.851133143096446	26.974600453841585
2	15.948346722654513	19.580297406113488	37.91773256501702	26.55362330621498
3	19.04767682184448	23.007753329940254	28.654259478432202	29.29031036978306
4	13.360398071288543	15.723051402575134	31.72405131338406	39.19249921275226
5	14.122221135142329	37.5303462403477	32.43582396841013	15.911608656099846
6	34.39960671736452	33.929913150084666	16.3013831653183	15.369096967232515
7	30.141195458930746	31.36019707900757	18.769227035259426	19.72938042680226
8	27.740140106375037	35.25834501695363	18.014352804281213	18.987162072390113
9	27.424439132703206	16.01216981933339	17.972627006564178	38.590764041399225
10-11	26.7305058654918	25.379509073123387	25.74352596816634	22.146459093218475
12-13	26.280731548324994	22.62969675413669	26.491192504858656	24.598379192679662
14-15	23.7513596080689	23.85551787039121	23.80622923431848	28.586893287221415
16-17	24.01936846243114	26.91714595441908	24.54258629151089	24.520899291638887
18-19	24.12634755131023	26.05457965741213	26.12722352411625	23.691849267161395
20-21	24.775865614609575	25.09082758198141	26.106173485079648	24.027133318329366
22-23	24.613986568012557	24.641102900719645	25.922516444205566	24.822394087062232
24-25	24.322501157145517	25.0318328760359	25.884086473803013	24.761579493015574
26-27	24.005567644330775	25.185401100304343	26.08118035515723	24.72785090020765
28-29	24.06805046913682	25.55844782957112	25.93631726230593	24.43718443898613
30-31	24.701401859804314	25.24812657685721	25.848295341147125	24.20217622219135
32-33	24.236450781429618	25.379097857203085	25.924093680559896	24.460357680807398
34-35	24.372730068737173	25.46633116018473	26.06501368252537	24.095925088552722
36-37	24.453472437491396	25.367268584545634	26.014481456250195	24.164777521712782
38-39	24.280825719629252	25.633093573186493	25.997404839567768	24.088675867616484
40-41	24.24624784570746	25.59223708531575	25.92676284977491	24.234752219201884
42-43	24.165566139889947	25.562815561013874	25.785933842214487	24.485684456881696
44-45	24.042450709847355	25.640888760553075	25.99767782278294	24.318982706816634
46-47	24.123708713563566	25.56548473022889	25.594966917467467	24.71583963874008
48-49	24.108967619944274	25.771071422721786	25.491415284512208	24.62854567282173
50-51	24.05452263425163	25.949450788102542	25.605067296428835	24.390959281216993
52-53	24.220587423481287	25.859032680943887	25.52935995142112	24.3910199441537
54-55	23.952881883802572	25.577283671417987	25.762790931861566	24.70704351291787
56-57	23.98630716192697	25.78232439748055	25.458566304286506	24.77280213630598
58-59	24.057434455213464	25.706465395131072	25.73643288586329	24.499667263792173
60-61	24.375156586205367	25.587323387442652	25.780292189100933	24.257227837251047
62-63	24.149581456068205	25.66509327229833	25.79636786732773	24.388957404305735
64-65	24.04202606929042	25.405334577327952	25.73109454743326	24.821544805948363
66-67	24.01008703311529	25.690875020397915	25.62026336207341	24.67877458441339
68-69	23.83471048310143	25.657662062551978	25.80810614558013	24.69952130876646
70-71	23.905504130236046	25.505155439675864	25.843442306210733	24.745898123877357
72-73	23.965560437573895	25.848810976109114	25.448860234413722	24.73676835190327
74-75	23.848298980923328	25.903346956206818	25.55932744215334	24.689026620716515
76-77	23.857216432618948	25.535881217116895	25.593177360834673	25.01372498942948
78-79	23.72005753272917	25.530815861902038	25.77371026046845	24.97541634490034
80-81	23.92054853853886	25.800735598770487	25.442126648439476	24.836589214251177
82-83	23.92255041545012	25.76412551646908	25.534091660484105	24.779232407596698
84-85	23.900529769426242	25.81356580988357	25.334844244876862	24.95106017581332
86-87	23.593271995015932	26.07041268389211	25.434058477857725	24.902256843234234
88-89	23.58866161182636	25.727303113889207	25.53670016676241	25.14733510752202
90-91	23.79582261871614	25.57649645211954	25.60039770717808	25.02728322198624
92-93	23.777386769907306	25.763347491107535	25.57495799833358	24.884307740651575
94-95	23.75980616966706	25.688373091846167	25.46743854234154	25.08438219614523
96-97	23.58594981035186	25.57961354547233	25.578946250942174	25.255490393233632
98-99	23.466614900944975	25.638561014041954	25.477076227552807	25.417747857460256
100-101	23.50455245008502	25.739678352977002	25.51862261162435	25.237146585313624
102-103	23.34106583566532	25.652505712932065	25.663758687690823	25.342669763711793
104-105	23.39545725460697	25.749665216316515	25.464397669935895	25.39047985914062
106-107	23.41094761081767	25.61808024624329	25.548803088475182	25.422169054463854
108-109	23.352987191273726	25.607898102850168	25.567678417224943	25.471436288651162
110-111	23.21065026012346	25.967576775245565	25.500986382342667	25.320786582288314
112-113	23.23952654674127	26.00183264010279	25.50648597478285	25.25215483837309
114-115	23.246070471719314	25.958833412144816	25.43173171621679	25.363364399919075
116-117	23.07821567273826	25.871428900743854	25.44730114859971	25.60305427791818
118-119	23.130894556034185	25.729883059773158	25.557963879986584	25.581258504206073
120-121	23.033803290244347	25.822242605018413	25.617626023007077	25.52632808173016
122-123	22.951273658632612	25.98781682222467	25.4122961950546	25.64861332408812
124-125	22.801489761755196	25.884049363903035	25.326829451761245	25.987631422580527
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	43.0
2	64.0
3	72.0
4	61.0
5	89.0
6	171.0
7	402.0
8	582.5
9	675.0
10	667.5
11	550.0
12	549.5
13	618.5
14	686.5
15	773.5
16	871.0
17	947.0
18	995.5
19	1064.0
20	1132.5
21	1227.0
22	1396.5
23	1546.5
24	1771.0
25	2166.0
26	2797.5
27	3627.5
28	4504.0
29	5693.0
30	7401.0
31	9378.5
32	11832.0
33	14468.5
34	17149.5
35	19902.0
36	23278.5
37	26897.5
38	30148.5
39	33807.5
40	37376.5
41	40253.5
42	43444.0
43	47655.5
44	52015.0
45	56446.0
46	60385.5
47	64149.0
48	68553.5
49	71983.0
50	74003.5
51	75352.0
52	75180.0
53	73914.5
54	73134.5
55	71226.0
56	66606.0
57	60534.0
58	54985.5
59	49439.5
60	42483.5
61	35284.0
62	28834.5
63	22573.5
64	16826.5
65	12706.0
66	9722.5
67	7466.0
68	5841.0
69	4431.5
70	3370.0
71	2647.0
72	2063.0
73	1594.0
74	1212.0
75	943.0
76	730.5
77	538.5
78	373.0
79	251.0
80	212.5
81	174.5
82	152.0
83	147.0
84	138.5
85	126.5
86	116.0
87	110.0
88	104.0
89	93.5
90	78.5
91	66.0
92	58.5
93	61.0
94	61.0
95	61.5
96	56.0
97	39.0
98	24.0
99	11.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33904515324367757
2	0.0
3	0.0
4	0.018562858631698934
5	0.17361732484941944
6	0.047499079439935504
7	0.023233904757976116
8	0.008128833518456396
9	0.006854911847653527
10-11	0.002456848936548388
12-13	0.05332272136360576
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.4265174681959388E-4
92-93	3.943090885818401E-4
94-95	6.066293670489847E-5
96-97	3.3364615187694163E-4
98-99	3.0331468352449235E-5
100-101	0.0
102-103	0.0
104-105	3.0331468352449235E-5
106-107	1.2132587340979694E-4
108-109	3.943090885818401E-4
110-111	3.033146835244923E-4
112-113	6.976237721063324E-4
114-115	2.7298321517204313E-4
116-117	6.672923037538833E-4
118-119	2.4265174681959388E-4
120-121	2.4265174681959388E-4
122-123	0.0019715454429092
124-125	9.09944050573477E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1648453.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.05475362196758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.29008808430255	55.017062475605826
2	9.15359751806305	12.092772576206174
3	2.8957633987148714	5.738368135488669
4	1.4324711086469755	3.784861046090508
5	0.8392216759422878	2.771729051929127
6	0.5346489295648816	2.1189661989994173
7	0.3743347015468011	1.73086105379787
8	0.26546914401202576	1.4028399121559192
9	0.18962728381516555	1.1273205161172313
>10	0.9761474892746697	11.138985914188797
>50	0.0379749830862518	1.6612941548432931
>100	0.010104632031264906	1.0090234501126318
>500	2.7552549962159387E-4	0.11653918745871991
>1k	2.7552549962159387E-4	0.2893763270057751
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1923	0.11665482728351977	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.066293670489847E-5	0.0	0.0	0.0	0.0
7	6.066293670489847E-5	0.0	0.0	0.0	0.0
8	6.066293670489847E-5	0.0	0.0	0.0	0.0
9	6.066293670489847E-5	0.0	0.0	0.0	6.066293670489847E-5
10-11	6.066293670489847E-5	0.0	0.0	0.0	6.066293670489847E-5
12-13	6.066293670489847E-5	0.0	0.0	0.0	6.066293670489847E-5
14-15	6.066293670489847E-5	0.0	0.0	0.0	9.09944050573477E-5
16-17	1.2132587340979694E-4	0.0	0.0	0.0	1.2132587340979694E-4
18-19	1.2132587340979694E-4	0.0	0.0	0.0	1.2132587340979694E-4
20-21	1.2132587340979694E-4	0.0	0.0	0.0	1.2132587340979694E-4
22-23	1.2132587340979694E-4	0.0	0.0	3.0331468352449235E-5	1.516573417622462E-4
24-25	1.2132587340979694E-4	0.0	0.0	1.819888101146954E-4	1.819888101146954E-4
26-27	1.2132587340979694E-4	0.0	0.0	4.549720252867385E-4	1.819888101146954E-4
28-29	1.2132587340979694E-4	0.0	0.0	0.0022445286580812436	1.819888101146954E-4
30-31	1.819888101146954E-4	0.0	0.0	0.009038777569029871	1.819888101146954E-4
32-33	1.819888101146954E-4	0.0	0.0	0.023537219441500605	1.819888101146954E-4
34-35	2.4265174681959388E-4	0.0	0.0	0.04131145989603586	1.819888101146954E-4
36-37	2.4265174681959388E-4	0.0	0.0	0.06760884295760934	1.819888101146954E-4
38-39	2.4265174681959388E-4	0.0	0.0	0.11207477556229993	1.819888101146954E-4
40-41	2.4265174681959388E-4	0.0	0.0	0.16785434586245407	1.819888101146954E-4
42-43	2.4265174681959388E-4	0.0	0.0	0.2164150266947253	1.819888101146954E-4
44-45	2.4265174681959388E-4	0.0	0.0	0.2692221130963394	1.819888101146954E-4
46-47	2.4265174681959388E-4	0.0	0.0	0.32339411557381376	2.4265174681959388E-4
48-49	2.4265174681959388E-4	0.0	0.0	0.38739351379748166	3.033146835244924E-4
50-51	2.4265174681959388E-4	0.0	0.0	0.45673125045118057	3.033146835244924E-4
52-53	2.4265174681959388E-4	0.0	0.0	0.528192189889551	3.033146835244924E-4
54-55	2.4265174681959388E-4	0.0	0.0	0.6077516313780253	3.033146835244924E-4
56-57	2.4265174681959388E-4	0.0	0.0	0.6887366518790647	3.3364615187694163E-4
58-59	2.4265174681959388E-4	0.0	0.0	0.7801556974933468	3.639776202293908E-4
60-61	2.4265174681959388E-4	0.0	0.0	0.8755784969301521	4.8530349363918777E-4
62-63	2.4265174681959388E-4	0.0	0.0	0.9804647144929215	4.8530349363918777E-4
64-65	2.4265174681959388E-4	0.0	0.0	1.0824997740305609	4.8530349363918777E-4
66-67	2.4265174681959388E-4	0.0	0.0	1.1951508474915573	4.8530349363918777E-4
68-69	2.4265174681959388E-4	0.0	0.0	1.3190852271796647	4.8530349363918777E-4
70-71	2.4265174681959388E-4	0.0	0.0	1.456092469727678	4.8530349363918777E-4
72-73	2.4265174681959388E-4	0.0	0.0	1.582969001845973	7.279552404587816E-4
74-75	2.4265174681959388E-4	0.0	0.0	1.7114834332553006	7.279552404587816E-4
76-77	2.4265174681959388E-4	0.0	0.0	1.860926577827818	7.279552404587816E-4
78-79	2.4265174681959388E-4	0.0	0.0	2.03308799219632	7.279552404587816E-4
80-81	2.4265174681959388E-4	0.0	0.0	2.2057650415268135	7.582867088112309E-4
82-83	2.4265174681959388E-4	0.0	0.0	2.3830828055152313	9.09944050573477E-4
84-85	2.4265174681959388E-4	0.0	0.0	2.5666185205159016	9.09944050573477E-4
86-87	2.4265174681959388E-4	0.0	0.0	2.773145488527729	9.706069872783755E-4
88-89	2.4265174681959388E-4	0.0	0.0	3.004271277373392	9.706069872783755E-4
90-91	2.4265174681959388E-4	0.0	0.0	3.244648164066552	9.706069872783755E-4
92-93	2.4265174681959388E-4	0.0	0.0	3.486966264734269	9.706069872783755E-4
94-95	2.4265174681959388E-4	0.0	0.0	3.7370795527685656	9.706069872783755E-4
96-97	2.4265174681959388E-4	0.0	0.0	4.012853263029034	0.001031269923983274
98-99	2.4265174681959388E-4	0.0	0.0	4.322749875186007	0.001031269923983274
100-101	2.4265174681959388E-4	0.0	0.0	4.653150559949237	0.001031269923983274
102-103	2.4265174681959388E-4	0.0	0.0	4.996957753724249	0.001031269923983274
104-105	2.7298321517204313E-4	0.0	0.0	5.35511173202997	0.001031269923983274
106-107	3.033146835244924E-4	0.0	0.0	5.727794483676513	0.0010616013923357233
108-109	3.033146835244924E-4	0.0	0.0	6.119980369473682	0.0010919328606881725
110-111	3.033146835244924E-4	0.0	0.0	6.558330750103279	0.0011222643290406218
112-113	3.033146835244924E-4	0.0	0.0	6.9997446090364726	0.001152595797393071
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1815	0.0	65.83291	1
GTATCAA	3170	0.0	50.131657	1
GTACATG	7740	0.0	41.141106	1
TACATGG	8110	0.0	39.236214	2
ACATGGG	8255	0.0	38.11391	3
CATGGGG	7480	0.0	36.008377	4
TCAACGC	4270	0.0	35.585983	4
TATCAAC	4455	0.0	35.579563	2
CAACGCA	4405	0.0	34.49538	5
ATCAACG	4560	0.0	33.453518	3
AACGCAG	4560	0.0	33.062492	6
ATGGGGG	4730	0.0	31.873228	5
TATACCG	215	3.972673E-9	27.715853	5
TGGGGGG	3235	0.0	26.157362	6
ACGCAGA	5760	0.0	25.649395	7
GTGGTAT	995	0.0	24.61787	1
GTATCGC	125	0.0016193738	23.836357	6
CGCAGAG	6155	0.0	23.51654	8
ATGGGGA	2750	0.0	22.752193	5
AGAGTAC	6105	0.0	22.547218	5
>>END_MODULE