FastQCFastQC Report
Mon 15 Aug 2016
SRR2921297_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921297_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397258
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG1600.052.0721632
GGTATCA12700.050.6150631
GTACGGT1251.2732926E-1147.6088374
GTATCAA18600.045.7596661
GTACATG62400.036.341271
ACATGGG64050.035.678753
TACATGG64000.035.5206572
TACGGTC1555.113179E-934.553565
TCAACGC23950.034.2902874
CAACGCA24150.034.0050935
CATGGGG59650.032.8233574
TATCAAC26850.031.4732532
AACGCAG26300.031.4503566
ATCAACG27000.030.8575783
ATGGGGG35050.027.8443985
GAGTACA43250.027.5234991
AGTACAT44400.026.6727452
AGTACGG2757.4578566E-1125.9684563
ACGCAGA32350.025.3782987
GTATAGG4300.024.9151671
Produced by FastQC (version 0.11.5)