FastQCFastQC Report
Mon 15 Aug 2016
SRR2921297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1397258
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.056.0303881
GTATCAA25000.048.5297851
GTACATG63150.037.667091
TCAACGC32750.035.6694644
TACATGG66450.035.249212
ACATGGG65300.035.1398053
CAACGCA33600.034.767115
TATCAAC35900.034.033862
ATCAACG35400.032.830933
AACGCAG35550.032.5130926
CATGGGG60650.032.4291044
ATGGGGG36250.028.6083665
ACGCAGA44750.025.8232827
GTGGTAT8500.025.3125511
CGCAGAG48900.023.3822448
TGGTATC9850.021.1779352
CTACACT6850.020.8820194
TACCGTA2004.867965E-520.8483077
ATGGGGA24050.020.8168985
TGGGGGG25800.020.7835126
Produced by FastQC (version 0.11.5)